Click here to switch to the map view.

The map label for this gene is amsH [H]

Identifier: 146282842

GI number: 146282842

Start: 2728993

End: 2730129

Strand: Direct

Name: amsH [H]

Synonym: PST_2501

Alternate gene names: 146282842

Gene position: 2728993-2730129 (Clockwise)

Preceding gene: 146282841

Following gene: 146282845

Centisome position: 59.75

GC content: 62.88

Gene sequence:

>1137_bases
TTGAACAACAACCACTCCGTCAAGGATGAACAAACCATGAAACCATTCATTGCACTCGCTTTTACTTCCGCTCTGACACT
GCAGGGCTGTGCTTTCGCCCCCGGCCAGTATCTTGACCCCGACGAGTTCAAGGAAGGCGCCGAAGCCGAACACGGCACGG
TGCAGCTGGTCCGTATCACGCCGGAAATGCTCCGGGGTGAGCTGGCTTCCGACCAGGGCACTTCGAGCAAGCAGGAACTG
CTCAGCTACAAGCCGAGCGCCTACCGCATCGGTGCCAACGACCTGCTCTACATCACCGTCTGGGATCACCCTGAACTGAC
CGCGCCGTCCGGCCCGCAGCAGCAGCTGGATGCCAACGGACGCCTGGTTCGCCCTGACGGCACGCTGTTCTACCCGTACA
TCGGCAACGTCGAAGCGGCCGGCAAGACCATCGAAGAGCTGCGTGCCACCATCGCCCGCCGTCTGACCAACTACATCGAC
AGTCCGCAGGTGGACGTTTCCATCCTGCGCTTCGGTAGCCAGCGCGTGGTCGTCTCCGGTGCGTTCAATACTGCCGGCGA
AGTACCGATCACCACCGCCCCGATGACCATCACCCAGGCCATCGGCCAGGCTGGCGTCGATACCGATACCGCCGACCTCA
CCGGTCTGACCCTCAAGCGTGATGGCCGCGAGTACATGCTGGACGTCGACTCCCTCAACCGCCCGGATTCCTGGCTGCAC
CAGGTCTACCTGAAGGACGGCGACCAGCTGCACCTTCCGTACAACGACCAGCGCAAGGTCTACGTGCTGGGTGAGGTAAT
GTCGCCGCAAGCCATGACCTTCAAGGCCACCAGCATGAACCTGATGGACGCGGTCGGCACCGCCGGCGGCATTCGCCAGG
AAACTGCCGATGGCGAGGCGCTGTACGTGATCCGCGGTGCGGAAAACATGGCCGAAGGTCAGGCCAAGGTGTTCCAGCTG
AACGCCAAGTCGCCGACCGCCTTCGCTCTGGCCAAGCACTTCCCGCTCCAGCCGCAGGATGTGGTGTTCGTGGGGCCGGC
CAACATCACCCGCTGGAACCGCTTCATCAGCCAGCTGCTGCCGTCCGCCAGCATCGTGGGCACCGGTGCCGCTGCCGAGT
ACAACCTGTCTCGCTAA

Upstream 100 bases:

>100_bases
GTCGCTGAGGAGTGTGGAGTGATGACTCCGGTCCTCTTGAAAAGGCGAAAGAACAATTCAAACGCCTCAGGCAAAAAAGT
GAATCAAGCCGTCTCACTCT

Downstream 100 bases:

>100_bases
GCGCGCCATGGCCTGATCGCAGGATCGGGTAGAAAAGCCGCAGAGTCATCACCGATGCTCTGCGGCTTTTTCGTATGTTG
GCACCCGCCATTGCGCCGTG

Product: capsular polysaccharide biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MNNNHSVKDEQTMKPFIALAFTSALTLQGCAFAPGQYLDPDEFKEGAEAEHGTVQLVRITPEMLRGELASDQGTSSKQEL
LSYKPSAYRIGANDLLYITVWDHPELTAPSGPQQQLDANGRLVRPDGTLFYPYIGNVEAAGKTIEELRATIARRLTNYID
SPQVDVSILRFGSQRVVVSGAFNTAGEVPITTAPMTITQAIGQAGVDTDTADLTGLTLKRDGREYMLDVDSLNRPDSWLH
QVYLKDGDQLHLPYNDQRKVYVLGEVMSPQAMTFKATSMNLMDAVGTAGGIRQETADGEALYVIRGAENMAEGQAKVFQL
NAKSPTAFALAKHFPLQPQDVVFVGPANITRWNRFISQLLPSASIVGTGAAAEYNLSR

Sequences:

>Translated_378_residues
MNNNHSVKDEQTMKPFIALAFTSALTLQGCAFAPGQYLDPDEFKEGAEAEHGTVQLVRITPEMLRGELASDQGTSSKQEL
LSYKPSAYRIGANDLLYITVWDHPELTAPSGPQQQLDANGRLVRPDGTLFYPYIGNVEAAGKTIEELRATIARRLTNYID
SPQVDVSILRFGSQRVVVSGAFNTAGEVPITTAPMTITQAIGQAGVDTDTADLTGLTLKRDGREYMLDVDSLNRPDSWLH
QVYLKDGDQLHLPYNDQRKVYVLGEVMSPQAMTFKATSMNLMDAVGTAGGIRQETADGEALYVIRGAENMAEGQAKVFQL
NAKSPTAFALAKHFPLQPQDVVFVGPANITRWNRFISQLLPSASIVGTGAAAEYNLSR
>Mature_378_residues
MNNNHSVKDEQTMKPFIALAFTSALTLQGCAFAPGQYLDPDEFKEGAEAEHGTVQLVRITPEMLRGELASDQGTSSKQEL
LSYKPSAYRIGANDLLYITVWDHPELTAPSGPQQQLDANGRLVRPDGTLFYPYIGNVEAAGKTIEELRATIARRLTNYID
SPQVDVSILRFGSQRVVVSGAFNTAGEVPITTAPMTITQAIGQAGVDTDTADLTGLTLKRDGREYMLDVDSLNRPDSWLH
QVYLKDGDQLHLPYNDQRKVYVLGEVMSPQAMTFKATSMNLMDAVGTAGGIRQETADGEALYVIRGAENMAEGQAKVFQL
NAKSPTAFALAKHFPLQPQDVVFVGPANITRWNRFISQLLPSASIVGTGAAAEYNLSR

Specific function: Involved in the biosynthesis of amylovoran which functions as a virulence factor. Might be involved in the translocation of polysaccharides across the outer membrane [H]

COG id: COG1596

COG function: function code M; Periplasmic protein involved in polysaccharide export

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BexD/CtrA/VexA family [H]

Homologues:

Organism=Escherichia coli, GI1787218, Length=352, Percent_Identity=37.7840909090909, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1788376, Length=372, Percent_Identity=37.3655913978495, Blast_Score=234, Evalue=9e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003715
- InterPro:   IPR019554 [H]

Pfam domain/function: PF02563 Poly_export; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 41215; Mature: 41215

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNHSVKDEQTMKPFIALAFTSALTLQGCAFAPGQYLDPDEFKEGAEAEHGTVQLVRIT
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEEC
PEMLRGELASDQGTSSKQELLSYKPSAYRIGANDLLYITVWDHPELTAPSGPQQQLDANG
HHHHHCHHCCCCCCCHHHHHHHCCCCEEEECCCCEEEEEEECCCCCCCCCCCHHHHCCCC
RLVRPDGTLFYPYIGNVEAAGKTIEELRATIARRLTNYIDSPQVDVSILRFGSQRVVVSG
EEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEE
AFNTAGEVPITTAPMTITQAIGQAGVDTDTADLTGLTLKRDGREYMLDVDSLNRPDSWLH
CCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCHHHHH
QVYLKDGDQLHLPYNDQRKVYVLGEVMSPQAMTFKATSMNLMDAVGTAGGIRQETADGEA
HHHCCCCCEEECCCCCCCEEEEEEECCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCE
LYVIRGAENMAEGQAKVFQLNAKSPTAFALAKHFPLQPQDVVFVGPANITRWNRFISQLL
EEEEECCCCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHC
PSASIVGTGAAAEYNLSR
CCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MNNNHSVKDEQTMKPFIALAFTSALTLQGCAFAPGQYLDPDEFKEGAEAEHGTVQLVRIT
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEEC
PEMLRGELASDQGTSSKQELLSYKPSAYRIGANDLLYITVWDHPELTAPSGPQQQLDANG
HHHHHCHHCCCCCCCHHHHHHHCCCCEEEECCCCEEEEEEECCCCCCCCCCCHHHHCCCC
RLVRPDGTLFYPYIGNVEAAGKTIEELRATIARRLTNYIDSPQVDVSILRFGSQRVVVSG
EEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEE
AFNTAGEVPITTAPMTITQAIGQAGVDTDTADLTGLTLKRDGREYMLDVDSLNRPDSWLH
CCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCHHHHH
QVYLKDGDQLHLPYNDQRKVYVLGEVMSPQAMTFKATSMNLMDAVGTAGGIRQETADGEA
HHHCCCCCEEECCCCCCCEEEEEEECCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCE
LYVIRGAENMAEGQAKVFQLNAKSPTAFALAKHFPLQPQDVVFVGPANITRWNRFISQLL
EEEEECCCCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHC
PSASIVGTGAAAEYNLSR
CCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7596293 [H]