Click here to switch to the map view.

The map label for this gene is wecG [H]

Identifier: 146282841

GI number: 146282841

Start: 2727817

End: 2728584

Strand: Direct

Name: wecG [H]

Synonym: PST_2500

Alternate gene names: 146282841

Gene position: 2727817-2728584 (Clockwise)

Preceding gene: 146282840

Following gene: 146282842

Centisome position: 59.72

GC content: 58.59

Gene sequence:

>768_bases
GTGTCTAACGAGACTAACCACGCGTTCGGTGTGAATTTCTACACCGGCAGCAAGGCGTCGCTGCTGAACTGCATTCGCGA
GGGGGTCAAGCAGCCCTACTCGTTCGTCGTGACACCCAACGTCGATCACCTCGCGCAGCTGCAGGACAACCCTGCCCTGC
GCGAGGCATACGCCAAGGCCCGGCTGCGCCTGTGCGACAGCCGGATCCTGCAGCCGCTGCTGCAGCGTCTCGGCGTGCAG
ATCGAGGAAGTCATTCCCGGCAGCGACCTGACCATGGACCTGCTCGACTGGGCCAATCGCGAGCATCTGCGCATCGTGCT
GATCGGCGCTACCGAACAGGAATGCAGCAAGTTGCGCGCGCTTTATCCGGGCATCACGGTCTACCACCACAATCCACCGA
TGGGCTTCATCAATCGACCGGATGAAGTGCAAAAGGCATTGCAGTTCGTTCGTGAACACCCCTCGGAACTGGTGTTCTAC
GCGGTGGGTGCACCGCGCCAGGAAATATTGGCCAGTTCGATCGAAAGTCACGAACGGACCGGAATGGGCTTTTGTATCGG
CGCGTCCATTTCGTTCGCCACCGGCAGTATTAAGCGCGCTCCGGTATGGATGCAGAACTGCAAACTGGAATGGCTGCATC
GCATGCTCTCCGAGCCAAAACGCCTGGTCAAACGCTATGTCCATGATGCGCTGTTCATTGTTCCGGCATTTTGGCGGGAG
AAGAACTACCGCATGGACAAAGCCAAGCCAGCAAAACAGGACAGCTGA

Upstream 100 bases:

>100_bases
TCGGCCTGTTTCCTGTCGGCGATCACCGTCATCAAGGAGTTCAACCACCCCAGCCTTGCCTGGTCCGGCATCAAGGGCGC
CTTCAAGGGAGTAACGATGA

Downstream 100 bases:

>100_bases
CGCATTCATCGAAAAAATGGCTGGAATTGCACGGAACCGCGGTGAACCCAGCCAGATCGAGACGGTCTATCCCTTCTGTG
TGAGCGTGACAGGATGGCAT

Product: N-acetyl-mannosamine transferase

Products: C55-PP-GlcNAc-ManNAcA; UDP [C]

Alternate protein names: UDP-ManNAcA transferase [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSNETNHAFGVNFYTGSKASLLNCIREGVKQPYSFVVTPNVDHLAQLQDNPALREAYAKARLRLCDSRILQPLLQRLGVQ
IEEVIPGSDLTMDLLDWANREHLRIVLIGATEQECSKLRALYPGITVYHHNPPMGFINRPDEVQKALQFVREHPSELVFY
AVGAPRQEILASSIESHERTGMGFCIGASISFATGSIKRAPVWMQNCKLEWLHRMLSEPKRLVKRYVHDALFIVPAFWRE
KNYRMDKAKPAKQDS

Sequences:

>Translated_255_residues
MSNETNHAFGVNFYTGSKASLLNCIREGVKQPYSFVVTPNVDHLAQLQDNPALREAYAKARLRLCDSRILQPLLQRLGVQ
IEEVIPGSDLTMDLLDWANREHLRIVLIGATEQECSKLRALYPGITVYHHNPPMGFINRPDEVQKALQFVREHPSELVFY
AVGAPRQEILASSIESHERTGMGFCIGASISFATGSIKRAPVWMQNCKLEWLHRMLSEPKRLVKRYVHDALFIVPAFWRE
KNYRMDKAKPAKQDS
>Mature_254_residues
SNETNHAFGVNFYTGSKASLLNCIREGVKQPYSFVVTPNVDHLAQLQDNPALREAYAKARLRLCDSRILQPLLQRLGVQI
EEVIPGSDLTMDLLDWANREHLRIVLIGATEQECSKLRALYPGITVYHHNPPMGFINRPDEVQKALQFVREHPSELVFYA
VGAPRQEILASSIESHERTGMGFCIGASISFATGSIKRAPVWMQNCKLEWLHRMLSEPKRLVKRYVHDALFIVPAFWREK
NYRMDKAKPAKQDS

Specific function: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II), the second lipid-linked intermediate involved in ECA synthesis [H]

COG id: COG1922

COG function: function code M; Teichoic acid biosynthesis proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 26 family [H]

Homologues:

Organism=Escherichia coli, GI2367289, Length=192, Percent_Identity=25, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023085
- InterPro:   IPR004629 [H]

Pfam domain/function: PF03808 Glyco_tran_WecB [H]

EC number: 2.4.1.- [C]

Molecular weight: Translated: 29086; Mature: 28955

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNETNHAFGVNFYTGSKASLLNCIREGVKQPYSFVVTPNVDHLAQLQDNPALREAYAKA
CCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCHHHHHHHHHH
RLRLCDSRILQPLLQRLGVQIEEVIPGSDLTMDLLDWANREHLRIVLIGATEQECSKLRA
HHHHHHHHHHHHHHHHHCCEEEHHCCCCCCHHHHHHCCCCCCEEEEEEECCHHHHHHHHH
LYPGITVYHHNPPMGFINRPDEVQKALQFVREHPSELVFYAVGAPRQEILASSIESHERT
HCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHC
GMGFCIGASISFATGSIKRAPVWMQNCKLEWLHRMLSEPKRLVKRYVHDALFIVPAFWRE
CCCEEEECCEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KNYRMDKAKPAKQDS
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNETNHAFGVNFYTGSKASLLNCIREGVKQPYSFVVTPNVDHLAQLQDNPALREAYAKA
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCHHHHHHHHHH
RLRLCDSRILQPLLQRLGVQIEEVIPGSDLTMDLLDWANREHLRIVLIGATEQECSKLRA
HHHHHHHHHHHHHHHHHCCEEEHHCCCCCCHHHHHHCCCCCCEEEEEEECCHHHHHHHHH
LYPGITVYHHNPPMGFINRPDEVQKALQFVREHPSELVFYAVGAPRQEILASSIESHERT
HCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHC
GMGFCIGASISFATGSIKRAPVWMQNCKLEWLHRMLSEPKRLVKRYVHDALFIVPAFWRE
CCCEEEECCEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KNYRMDKAKPAKQDS
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosaminuronic-acid; undecaprenyl-N-acetyl-alpha-D-glucosaminyl-pyrophosphate [C]

Specific reaction: UDP-N-acetyl-D-mannosaminuronic-acid + undecaprenyl-N-acetyl-alpha-D-glucosaminyl-pyrophosphate = C55-PP-GlcNAc-ManNAcA + UDP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA