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The map label for this gene is insF1 [H]

Identifier: 146282663

GI number: 146282663

Start: 2527620

End: 2528465

Strand: Direct

Name: insF1 [H]

Synonym: PST_2315

Alternate gene names: 146282663

Gene position: 2527620-2528465 (Clockwise)

Preceding gene: 146282662

Following gene: 146282666

Centisome position: 55.34

GC content: 59.93

Gene sequence:

>846_bases
GTGGAGTACCCGGTACGGCGTCTCTGCCAGACCCTCCAGGTACATCCCAGCGGTTACTACGCCTGGCTGGCCGAGCCGAA
ATCCGCGCGTGCCAAGGAAGATCAGCGTCTGCTCGGATTGATCAAGCATGCCTGGTTGGAAAGCGGCGGGGTCTACGGCT
ACCGCAAGACCCACGACGACCTGCGTGAGCTGGGCGAGTCCTGTGGCCGGCACCGTGTGGCTCGCCTGATGAGGAGAGAG
GGGCTGCGCTCGCAGACCGGTTATCGCCGGCGCCCCGGGTACTACGGTGGCAAGCCGCCGGTGGCCTCGCCCAACCGTCT
GGAGCGGCAGTTCAATGTCAGTGAACCGAACAAGGTCTGGGTCACCGACATCACTTACATCCGCACGTATGAGGGCTGGT
TGTACTTGGCGGTGGTGCTGGATCTGTTTTCTCGCCAAGTGATCGGCTGGTCGATGAAGCCTCGGATGTGCAGCGATCTG
GCCATCGATGCGTTGCTGATGGCGGTGTGGCGGCGCAAGCCCAAGCAGGAAGTGATGATCCATTCCGACCAGGGCAGCCA
GTTCAGCAGCTCGGACTGGCAGAGTTTCCTCAAGGCCAACAACCTGATCAGCAGCATGAGTCGACGCGGTAACTGTCACG
ACAACGCGGTCGCGGAAAGCTTTTTCCAGTTGCTGAAGCGGGAACGCATCCGACGGAAAACCTACGGAACCCGCGAAGAA
GCCCGCAGTGATGTGTTCGATTACATCGAGATGTTCTATAACCCCAAGCGACGGCACAGCAGCGCTATGCAGCTATCGCC
AGTGGAGTTTGAAAAGCGCTATTTCCAGAGCTTGGAGAGTGTCTAG

Upstream 100 bases:

>100_bases
AACTCAAGCGGGTGACCGAAGAGCGAGACATCCTAAAAAAGGCCGCCGCGTACTTTGCCAAGGAGTCCGGCTGAAGTACG
CCGTTATCAGCAAACTGTCG

Downstream 100 bases:

>100_bases
GAAAGCTGGGGCGATTCAGACGACTGATTCGCGACAGGGGCCGTTGTTGTCCGAACGGCCCTTCTCTTCGGTTCAGAGGG
TCATCGCTGCCAGCCAGCCG

Product: IS3 family transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MEYPVRRLCQTLQVHPSGYYAWLAEPKSARAKEDQRLLGLIKHAWLESGGVYGYRKTHDDLRELGESCGRHRVARLMRRE
GLRSQTGYRRRPGYYGGKPPVASPNRLERQFNVSEPNKVWVTDITYIRTYEGWLYLAVVLDLFSRQVIGWSMKPRMCSDL
AIDALLMAVWRRKPKQEVMIHSDQGSQFSSSDWQSFLKANNLISSMSRRGNCHDNAVAESFFQLLKRERIRRKTYGTREE
ARSDVFDYIEMFYNPKRRHSSAMQLSPVEFEKRYFQSLESV

Sequences:

>Translated_281_residues
MEYPVRRLCQTLQVHPSGYYAWLAEPKSARAKEDQRLLGLIKHAWLESGGVYGYRKTHDDLRELGESCGRHRVARLMRRE
GLRSQTGYRRRPGYYGGKPPVASPNRLERQFNVSEPNKVWVTDITYIRTYEGWLYLAVVLDLFSRQVIGWSMKPRMCSDL
AIDALLMAVWRRKPKQEVMIHSDQGSQFSSSDWQSFLKANNLISSMSRRGNCHDNAVAESFFQLLKRERIRRKTYGTREE
ARSDVFDYIEMFYNPKRRHSSAMQLSPVEFEKRYFQSLESV
>Mature_281_residues
MEYPVRRLCQTLQVHPSGYYAWLAEPKSARAKEDQRLLGLIKHAWLESGGVYGYRKTHDDLRELGESCGRHRVARLMRRE
GLRSQTGYRRRPGYYGGKPPVASPNRLERQFNVSEPNKVWVTDITYIRTYEGWLYLAVVLDLFSRQVIGWSMKPRMCSDL
AIDALLMAVWRRKPKQEVMIHSDQGSQFSSSDWQSFLKANNLISSMSRRGNCHDNAVAESFFQLLKRERIRRKTYGTREE
ARSDVFDYIEMFYNPKRRHSSAMQLSPVEFEKRYFQSLESV

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=242, Percent_Identity=36.7768595041322, Blast_Score=148, Evalue=5e-36,
Organism=Escherichia coli, GI1788405, Length=274, Percent_Identity=44.8905109489051, Blast_Score=224, Evalue=5e-60,
Organism=Escherichia coli, GI1787263, Length=274, Percent_Identity=44.8905109489051, Blast_Score=224, Evalue=5e-60,
Organism=Escherichia coli, GI1786753, Length=274, Percent_Identity=44.8905109489051, Blast_Score=224, Evalue=5e-60,
Organism=Escherichia coli, GI1786570, Length=274, Percent_Identity=44.8905109489051, Blast_Score=224, Evalue=5e-60,
Organism=Escherichia coli, GI1786490, Length=274, Percent_Identity=44.8905109489051, Blast_Score=224, Evalue=5e-60,
Organism=Escherichia coli, GI1789981, Length=269, Percent_Identity=33.457249070632, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 33034; Mature: 33034

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYPVRRLCQTLQVHPSGYYAWLAEPKSARAKEDQRLLGLIKHAWLESGGVYGYRKTHDD
CCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LRELGESCGRHRVARLMRREGLRSQTGYRRRPGYYGGKPPVASPNRLERQFNVSEPNKVW
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEE
VTDITYIRTYEGWLYLAVVLDLFSRQVIGWSMKPRMCSDLAIDALLMAVWRRKPKQEVMI
EEEHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHEEE
HSDQGSQFSSSDWQSFLKANNLISSMSRRGNCHDNAVAESFFQLLKRERIRRKTYGTREE
ECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHH
ARSDVFDYIEMFYNPKRRHSSAMQLSPVEFEKRYFQSLESV
HHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MEYPVRRLCQTLQVHPSGYYAWLAEPKSARAKEDQRLLGLIKHAWLESGGVYGYRKTHDD
CCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LRELGESCGRHRVARLMRREGLRSQTGYRRRPGYYGGKPPVASPNRLERQFNVSEPNKVW
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEE
VTDITYIRTYEGWLYLAVVLDLFSRQVIGWSMKPRMCSDLAIDALLMAVWRRKPKQEVMI
EEEHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHEEE
HSDQGSQFSSSDWQSFLKANNLISSMSRRGNCHDNAVAESFFQLLKRERIRRKTYGTREE
ECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHH
ARSDVFDYIEMFYNPKRRHSSAMQLSPVEFEKRYFQSLESV
HHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]