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The map label for this gene is cspD [H]

Identifier: 146282648

GI number: 146282648

Start: 2510703

End: 2510984

Strand: Direct

Name: cspD [H]

Synonym: PST_2300

Alternate gene names: 146282648

Gene position: 2510703-2510984 (Clockwise)

Preceding gene: 146282645

Following gene: 146282650

Centisome position: 54.97

GC content: 58.87

Gene sequence:

>282_bases
TTGCAGAGGGATAACAGCATGCTAAGCGGTAAGGTCAAGTGGTTCAACAACGCCAAAGGCTACGGCTTCATCGTAGCAGA
CGGCGGCGATGAGGACCTGTTCGCCCACTACTCGGCCATCCAGATGGAAGGCTACAGAACTCTGAAAGCTGGGCAAGCGG
TCATGTTCAATATCCTGCAGGGTCCGAAAGGCCTCCACGCAACCGACATCCGGCCTCAGCAAGCCGTGTCCGAAGCGACG
ACTCCTGCCGCTGCCCAGGGTGCTTCCACGGTCGACGCCTGA

Upstream 100 bases:

>100_bases
AAACAGTAAGTGCCTACAAGGGCAAACGGGTTGCGCAACATGAGGCCTCGGGCTGCATAAGCGCGGAATGAAATGGATGT
TACTCCAGTAATGGACTCCT

Downstream 100 bases:

>100_bases
CGTCCTGCGGGAGCGAACAAAAAAGGCCGATCAATCGATCGGCCTTTTTACCCACTTGCTTACATGTGGCTGATCATGGC
GTCGCCGAACTGCGAGCATG

Product: cold-shock protein CspD

Products: NA

Alternate protein names: CSP-D [H]

Number of amino acids: Translated: 93; Mature: 93

Protein sequence:

>93_residues
MQRDNSMLSGKVKWFNNAKGYGFIVADGGDEDLFAHYSAIQMEGYRTLKAGQAVMFNILQGPKGLHATDIRPQQAVSEAT
TPAAAQGASTVDA

Sequences:

>Translated_93_residues
MQRDNSMLSGKVKWFNNAKGYGFIVADGGDEDLFAHYSAIQMEGYRTLKAGQAVMFNILQGPKGLHATDIRPQQAVSEAT
TPAAAQGASTVDA
>Mature_93_residues
MQRDNSMLSGKVKWFNNAKGYGFIVADGGDEDLFAHYSAIQMEGYRTLKAGQAVMFNILQGPKGLHATDIRPQQAVSEAT
TPAAAQGASTVDA

Specific function: Inhibits DNA replication at both initiation and elongation steps, most probably by binding to the opened, single- stranded regions at replication forks. Plays a regulatory role in chromosomal replication in nutrient-depleted cells [H]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=66, Percent_Identity=69.6969696969697, Blast_Score=103, Evalue=2e-24,
Organism=Escherichia coli, GI1786841, Length=65, Percent_Identity=49.2307692307692, Blast_Score=74, Evalue=2e-15,
Organism=Escherichia coli, GI1789979, Length=63, Percent_Identity=50.7936507936508, Blast_Score=70, Evalue=2e-14,
Organism=Escherichia coli, GI1788126, Length=63, Percent_Identity=49.2063492063492, Blast_Score=69, Evalue=5e-14,
Organism=Escherichia coli, GI2367114, Length=63, Percent_Identity=47.6190476190476, Blast_Score=69, Evalue=8e-14,
Organism=Escherichia coli, GI1787839, Length=63, Percent_Identity=50.7936507936508, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI1787834, Length=63, Percent_Identity=47.6190476190476, Blast_Score=67, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012751
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 9934; Mature: 9934

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRDNSMLSGKVKWFNNAKGYGFIVADGGDEDLFAHYSAIQMEGYRTLKAGQAVMFNILQ
CCCCCCCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHEEEECCCEEECCCHHHHHHHHC
GPKGLHATDIRPQQAVSEATTPAAAQGASTVDA
CCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQRDNSMLSGKVKWFNNAKGYGFIVADGGDEDLFAHYSAIQMEGYRTLKAGQAVMFNILQ
CCCCCCCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHEEEECCCEEECCCHHHHHHHHC
GPKGLHATDIRPQQAVSEATTPAAAQGASTVDA
CCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]