Click here to switch to the map view.

The map label for this gene is gloB [H]

Identifier: 146282602

GI number: 146282602

Start: 2456932

End: 2457810

Strand: Direct

Name: gloB [H]

Synonym: PST_2254

Alternate gene names: 146282602

Gene position: 2456932-2457810 (Clockwise)

Preceding gene: 146282594

Following gene: 146282603

Centisome position: 53.79

GC content: 65.64

Gene sequence:

>879_bases
ATGGGACTGCCAGAGCCCGCTCCCGTTTCCGGCGCGGGTTGCAGCCTGGCGGGAACAGCCCCTAGGATGCGCCTTTGTGA
TCCGCTTGGCGACCCATCCATGTTCAAGATCGAAGCCCTGCCCGCCTTCACCGACAACTATATCTGGCTGCTCCAGGACG
ACGCTGCCCGGCGTTGCGTGGCGGTCGATCCCGGTGACGCAGCGCCCGTGCTGAACTGGCTGTCGGCACATCCCGGCTGG
CAGCTGAGCGACATTCTGGTGACGCACCACCATCACGACCATGTCGGTGGCGTCGAGCGGCTCAAGGCACAGACCGGCGC
CCGCGTCTACGGGCCTGCCGCCGAGAAGATCCCCGCGCGTGACGAGGCGCTGAGCGACGGCCAACGGATCGAGGTGCTCG
GCAAGCGCCTGCAGATCATCGCCGTCCCAGGGCACACCCTCGGACATATCGCCTACTTTCACGCTGACCCAGACCAGCCA
TGGCTGCTCAGTGGCGACACGCTGTTCGCAGCCGGTTGTGGACGCCTTTTCGAAGGCACGCCCGAGCAGATGTTCGAGTC
GCTCACCCGCCTGGCCAGCGCCCCCGACCACACGCTGGTCTATTGCACGCATGAGTACACGCTGAGCAATCTGCGTTTCG
CCCAGGCGGTTGAGCCACACAACGCCGACATCGCCGCGCGCATCGGCGAAGTAACCCAGCTGCGCGAGGCAGGTCGATTC
AGCCTGCCCAGCCGACTGGACATCGAGCGCAAGACCAACCCGTTTCTGCGCAGCGAGGTCGAAGCGGTACGGCAGGCGGC
GAGCCAGCATTGCGGCAGTCCGCTGGCGACCAACGTCGCGGTTTTCGCGGCTTTGCGTGCCTGGAAGGATCGTTTCTGA

Upstream 100 bases:

>100_bases
CAATGGTCGCTGGCGTGGTTGGACGGTCGCTCGTTCATCGCTCGGCTCCGTTCGCCCGAGGATTCGCCGGGCGCTGGGTT
CGATCAGGTTCAACGCTTCC

Downstream 100 bases:

>100_bases
CAAGGTCGACAGGTTCCCGAAACGGGGCCTGCAAACTTGACCCCACCTGGGTGCATTCCTAGAATCCCCCGAATTTTGTT
TCCGGGGAAGATTCCAGCCA

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MGLPEPAPVSGAGCSLAGTAPRMRLCDPLGDPSMFKIEALPAFTDNYIWLLQDDAARRCVAVDPGDAAPVLNWLSAHPGW
QLSDILVTHHHHDHVGGVERLKAQTGARVYGPAAEKIPARDEALSDGQRIEVLGKRLQIIAVPGHTLGHIAYFHADPDQP
WLLSGDTLFAAGCGRLFEGTPEQMFESLTRLASAPDHTLVYCTHEYTLSNLRFAQAVEPHNADIAARIGEVTQLREAGRF
SLPSRLDIERKTNPFLRSEVEAVRQAASQHCGSPLATNVAVFAALRAWKDRF

Sequences:

>Translated_292_residues
MGLPEPAPVSGAGCSLAGTAPRMRLCDPLGDPSMFKIEALPAFTDNYIWLLQDDAARRCVAVDPGDAAPVLNWLSAHPGW
QLSDILVTHHHHDHVGGVERLKAQTGARVYGPAAEKIPARDEALSDGQRIEVLGKRLQIIAVPGHTLGHIAYFHADPDQP
WLLSGDTLFAAGCGRLFEGTPEQMFESLTRLASAPDHTLVYCTHEYTLSNLRFAQAVEPHNADIAARIGEVTQLREAGRF
SLPSRLDIERKTNPFLRSEVEAVRQAASQHCGSPLATNVAVFAALRAWKDRF
>Mature_291_residues
GLPEPAPVSGAGCSLAGTAPRMRLCDPLGDPSMFKIEALPAFTDNYIWLLQDDAARRCVAVDPGDAAPVLNWLSAHPGWQ
LSDILVTHHHHDHVGGVERLKAQTGARVYGPAAEKIPARDEALSDGQRIEVLGKRLQIIAVPGHTLGHIAYFHADPDQPW
LLSGDTLFAAGCGRLFEGTPEQMFESLTRLASAPDHTLVYCTHEYTLSNLRFAQAVEPHNADIAARIGEVTQLREAGRFS
LPSRLDIERKTNPFLRSEVEAVRQAASQHCGSPLATNVAVFAALRAWKDRF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538322, Length=267, Percent_Identity=38.2022471910112, Blast_Score=178, Evalue=4e-45,
Organism=Homo sapiens, GI94538320, Length=263, Percent_Identity=38.4030418250951, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI14150041, Length=235, Percent_Identity=38.7234042553192, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI116642887, Length=242, Percent_Identity=37.603305785124, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI46361987, Length=198, Percent_Identity=38.8888888888889, Blast_Score=137, Evalue=8e-33,
Organism=Homo sapiens, GI21703352, Length=242, Percent_Identity=37.603305785124, Blast_Score=137, Evalue=1e-32,
Organism=Escherichia coli, GI1786406, Length=258, Percent_Identity=46.8992248062016, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1787158, Length=213, Percent_Identity=29.1079812206573, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17536925, Length=266, Percent_Identity=33.4586466165414, Blast_Score=137, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6320478, Length=271, Percent_Identity=30.9963099630996, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324614, Length=271, Percent_Identity=28.7822878228782, Blast_Score=89, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24667711, Length=264, Percent_Identity=36.7424242424242, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI21356335, Length=265, Percent_Identity=37.3584905660377, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24667703, Length=265, Percent_Identity=37.3584905660377, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI221330176, Length=220, Percent_Identity=27.7272727272727, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 31817; Mature: 31686

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLPEPAPVSGAGCSLAGTAPRMRLCDPLGDPSMFKIEALPAFTDNYIWLLQDDAARRCV
CCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCEEE
AVDPGDAAPVLNWLSAHPGWQLSDILVTHHHHDHVGGVERLKAQTGARVYGPAAEKIPAR
EECCCCHHHHHHHHHCCCCCCHHHEEEECCCCCHHHHHHHHHHHCCCEEECCCHHCCCCC
DEALSDGQRIEVLGKRLQIIAVPGHTLGHIAYFHADPDQPWLLSGDTLFAAGCGRLFEGT
HHHHCCCCHHHEECCEEEEEEECCCCEEEEEEEECCCCCCEEEECCEEEECCCCHHHCCC
PEQMFESLTRLASAPDHTLVYCTHEYTLSNLRFAQAVEPHNADIAARIGEVTQLREAGRF
HHHHHHHHHHHHCCCCCEEEEEECCHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
SLPSRLDIERKTNPFLRSEVEAVRQAASQHCGSPLATNVAVFAALRAWKDRF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GLPEPAPVSGAGCSLAGTAPRMRLCDPLGDPSMFKIEALPAFTDNYIWLLQDDAARRCV
CCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCEEE
AVDPGDAAPVLNWLSAHPGWQLSDILVTHHHHDHVGGVERLKAQTGARVYGPAAEKIPAR
EECCCCHHHHHHHHHCCCCCCHHHEEEECCCCCHHHHHHHHHHHCCCEEECCCHHCCCCC
DEALSDGQRIEVLGKRLQIIAVPGHTLGHIAYFHADPDQPWLLSGDTLFAAGCGRLFEGT
HHHHCCCCHHHEECCEEEEEEECCCCEEEEEEEECCCCCCEEEECCEEEECCCCHHHCCC
PEQMFESLTRLASAPDHTLVYCTHEYTLSNLRFAQAVEPHNADIAARIGEVTQLREAGRF
HHHHHHHHHHHHCCCCCEEEEEECCHHHHHCHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
SLPSRLDIERKTNPFLRSEVEAVRQAASQHCGSPLATNVAVFAALRAWKDRF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA