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The map label for this gene is mltD [H]

Identifier: 146282603

GI number: 146282603

Start: 2457911

End: 2459464

Strand: Direct

Name: mltD [H]

Synonym: PST_2255

Alternate gene names: 146282603

Gene position: 2457911-2459464 (Clockwise)

Preceding gene: 146282602

Following gene: 146282605

Centisome position: 53.81

GC content: 63.32

Gene sequence:

>1554_bases
ATGCCGCCAGCTCCCAAGAAACGCCACGAAACGGACGCCTTGGCAGCCTCTGGTCGTGCGTTGGCCCTCGCCATTTGCGT
AGCCCTAGCCGGCTGTCAGAGCAACGCCGTGCGTGACGATGCTCTCGAACAGACAAGGCGCGCAGTGGTGCCTGCGGCAC
AGGAGCCGATCTGGCTGAGCGACACCGCCACCGAGGTCGAGCACCAGGATATCTGGGAGCGAATCCGCGACGGCTACAAG
CTGCAGGAGCATCTGGACAGCAACCCCCGCATCGAACAGCAGCGTCTCTTCTTCTCCAGCCGCCCCCGATCCATAGAGAT
CGCCAGCGAGCGCGGCAGCCCCTACATCCATTACATCGTCGAGCGGCTCGAAGAGCGCGACATGCCGCTGGAGCTCGCGT
TGCTTCCGATCATCGAAAGCTCCTATGACCCCTTCGCCTACTCGCCGGCGCACGCGGTCGGGCTCTGGCAGTTCATTCCA
TCCACCGGCCGGCACTTCAACCTGCGCCAGACCAGCTGGTACGACGGCCGCCGCGACATCACCGCCTCGACCAATGCGGC
CTTGCGCTATCTGAGCTATCTGCATGGGCTGTTCAACGGTGACTGGCTGCTGGCACTGGCGGCCTACAACGCAGGCGAAG
GCACCGTCAGCCGCGCCATCGAGCGCAACCAGAAGCTGGGCCTGCCCACCGATTACTGGAACCTGCCGCTGCCGCAGGAA
ACCCGCGACTATGTGCCCAAGCTGCTGGCGCTGTCGCAACTGATCCAGGCACCGGAAGCCTATGGCATCAATCTCAGCCC
GATCGCCAACGAACCCTATTTCGAAGTCATCGCACTCAAGCAGCGCATGGACCTGGCACGCGTCGCCAAACTCGCCGACC
TCGACGAGGACGAGCTCTACCAGCTGAACCCGGCGTTCACCCGGCGGATCACCCTGGACGGCCCGCAGCAGCTGCTCGTA
CCCATGGAGAAAGCCGAGATGCTGGCGGCCAACCTCGCCCTGATGAAGCCCCAGGAGCTGGTCGACTGGCAGCAGTATCA
GGTTCGCGCCGGCGATACGCTGGGGACGATCGCCAACCGTCACCACCTCACCGTCAATATCATTCGCGACGTCAATCGAC
TCAAGAGCGACTCGCTGCGCGTCGGTCAGGTACTCAGCCTTCCCACCAGTGGCGATGCCCAGGCGTCGCGCGAGCTGTTG
CATGCCGTTGCACGTCAGCCGGCCGCGACTCCGCGTAGCTATAGGGTGAAGCCCGGCGACAACCTCTGGGACATCGCCAA
GGCCCAGCGCGTATCGGTTCGGGACCTGCAGCGCTGGAACAAGCTGTCCGGCAGCCAGCTCAAGGTCGGCCAGGCGCTGT
TCCTGCAGGGCCCGGCAGCAAGCGTTGCGCAAAAGAAAGGCAATGCCCCTACCTATTACAAGGTGCAGAAAGGCGATTCG
CTGTACCAGATCGCCAAACGTTTCAACGTTCAGTTGACCAACCTCAAGACCTGGAACAAGGGCGCCGACGTGCTGCGCCC
TGGCCAACTGCTGACGCTGTATCTCCCCAACTGA

Upstream 100 bases:

>100_bases
CAAGGTCGACAGGTTCCCGAAACGGGGCCTGCAAACTTGACCCCACCTGGGTGCATTCCTAGAATCCCCCGAATTTTGTT
TCCGGGGAAGATTCCAGCCA

Downstream 100 bases:

>100_bases
AGGATTCAGCCGCCAGGGCGCTTGCCCAGTGACTCCAGGCAGCGTCCGGTCAGCTGGGTGAAGCGCTGGAACGCAACGAA
CTGCTCGTGCTTGAGCGCCC

Product: membrane-bound lytic murein transglycosylase D precursor

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MPPAPKKRHETDALAASGRALALAICVALAGCQSNAVRDDALEQTRRAVVPAAQEPIWLSDTATEVEHQDIWERIRDGYK
LQEHLDSNPRIEQQRLFFSSRPRSIEIASERGSPYIHYIVERLEERDMPLELALLPIIESSYDPFAYSPAHAVGLWQFIP
STGRHFNLRQTSWYDGRRDITASTNAALRYLSYLHGLFNGDWLLALAAYNAGEGTVSRAIERNQKLGLPTDYWNLPLPQE
TRDYVPKLLALSQLIQAPEAYGINLSPIANEPYFEVIALKQRMDLARVAKLADLDEDELYQLNPAFTRRITLDGPQQLLV
PMEKAEMLAANLALMKPQELVDWQQYQVRAGDTLGTIANRHHLTVNIIRDVNRLKSDSLRVGQVLSLPTSGDAQASRELL
HAVARQPAATPRSYRVKPGDNLWDIAKAQRVSVRDLQRWNKLSGSQLKVGQALFLQGPAASVAQKKGNAPTYYKVQKGDS
LYQIAKRFNVQLTNLKTWNKGADVLRPGQLLTLYLPN

Sequences:

>Translated_517_residues
MPPAPKKRHETDALAASGRALALAICVALAGCQSNAVRDDALEQTRRAVVPAAQEPIWLSDTATEVEHQDIWERIRDGYK
LQEHLDSNPRIEQQRLFFSSRPRSIEIASERGSPYIHYIVERLEERDMPLELALLPIIESSYDPFAYSPAHAVGLWQFIP
STGRHFNLRQTSWYDGRRDITASTNAALRYLSYLHGLFNGDWLLALAAYNAGEGTVSRAIERNQKLGLPTDYWNLPLPQE
TRDYVPKLLALSQLIQAPEAYGINLSPIANEPYFEVIALKQRMDLARVAKLADLDEDELYQLNPAFTRRITLDGPQQLLV
PMEKAEMLAANLALMKPQELVDWQQYQVRAGDTLGTIANRHHLTVNIIRDVNRLKSDSLRVGQVLSLPTSGDAQASRELL
HAVARQPAATPRSYRVKPGDNLWDIAKAQRVSVRDLQRWNKLSGSQLKVGQALFLQGPAASVAQKKGNAPTYYKVQKGDS
LYQIAKRFNVQLTNLKTWNKGADVLRPGQLLTLYLPN
>Mature_516_residues
PPAPKKRHETDALAASGRALALAICVALAGCQSNAVRDDALEQTRRAVVPAAQEPIWLSDTATEVEHQDIWERIRDGYKL
QEHLDSNPRIEQQRLFFSSRPRSIEIASERGSPYIHYIVERLEERDMPLELALLPIIESSYDPFAYSPAHAVGLWQFIPS
TGRHFNLRQTSWYDGRRDITASTNAALRYLSYLHGLFNGDWLLALAAYNAGEGTVSRAIERNQKLGLPTDYWNLPLPQET
RDYVPKLLALSQLIQAPEAYGINLSPIANEPYFEVIALKQRMDLARVAKLADLDEDELYQLNPAFTRRITLDGPQQLLVP
MEKAEMLAANLALMKPQELVDWQQYQVRAGDTLGTIANRHHLTVNIIRDVNRLKSDSLRVGQVLSLPTSGDAQASRELLH
AVARQPAATPRSYRVKPGDNLWDIAKAQRVSVRDLQRWNKLSGSQLKVGQALFLQGPAASVAQKKGNAPTYYKVQKGDSL
YQIAKRFNVQLTNLKTWNKGADVLRPGQLLTLYLPN

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=511, Percent_Identity=35.2250489236791, Blast_Score=286, Evalue=3e-78,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 58135; Mature: 58004

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPAPKKRHETDALAASGRALALAICVALAGCQSNAVRDDALEQTRRAVVPAAQEPIWLS
CCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEC
DTATEVEHQDIWERIRDGYKLQEHLDSNPRIEQQRLFFSSRPRSIEIASERGSPYIHYIV
CCHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
ERLEERDMPLELALLPIIESSYDPFAYSPAHAVGLWQFIPSTGRHFNLRQTSWYDGRRDI
HHHHHCCCCEEEEEEHHHHCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCC
TASTNAALRYLSYLHGLFNGDWLLALAAYNAGEGTVSRAIERNQKLGLPTDYWNLPLPQE
CCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHH
TRDYVPKLLALSQLIQAPEAYGINLSPIANEPYFEVIALKQRMDLARVAKLADLDEDELY
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHEE
QLNPAFTRRITLDGPQQLLVPMEKAEMLAANLALMKPQELVDWQQYQVRAGDTLGTIANR
EECHHHEEEEECCCHHHHHCCHHHHHHHHHHHHHCCCHHHHCHHHHHEECCCHHHHHCCC
HHLTVNIIRDVNRLKSDSLRVGQVLSLPTSGDAQASRELLHAVARQPAATPRSYRVKPGD
CEEEEHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCC
NLWDIAKAQRVSVRDLQRWNKLSGSQLKVGQALFLQGPAASVAQKKGNAPTYYKVQKGDS
CHHHHHHHHHCHHHHHHHHHCCCCCEEECCCEEEECCCHHHHHHHCCCCCCEEEEECCCH
LYQIAKRFNVQLTNLKTWNKGADVLRPGQLLTLYLPN
HHHHHHHHCEEEEEEECCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
PPAPKKRHETDALAASGRALALAICVALAGCQSNAVRDDALEQTRRAVVPAAQEPIWLS
CCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEC
DTATEVEHQDIWERIRDGYKLQEHLDSNPRIEQQRLFFSSRPRSIEIASERGSPYIHYIV
CCHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
ERLEERDMPLELALLPIIESSYDPFAYSPAHAVGLWQFIPSTGRHFNLRQTSWYDGRRDI
HHHHHCCCCEEEEEEHHHHCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCC
TASTNAALRYLSYLHGLFNGDWLLALAAYNAGEGTVSRAIERNQKLGLPTDYWNLPLPQE
CCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHH
TRDYVPKLLALSQLIQAPEAYGINLSPIANEPYFEVIALKQRMDLARVAKLADLDEDELY
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHEE
QLNPAFTRRITLDGPQQLLVPMEKAEMLAANLALMKPQELVDWQQYQVRAGDTLGTIANR
EECHHHEEEEECCCHHHHHCCHHHHHHHHHHHHHCCCHHHHCHHHHHEECCCHHHHHCCC
HHLTVNIIRDVNRLKSDSLRVGQVLSLPTSGDAQASRELLHAVARQPAATPRSYRVKPGD
CEEEEHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCC
NLWDIAKAQRVSVRDLQRWNKLSGSQLKVGQALFLQGPAASVAQKKGNAPTYYKVQKGDS
CHHHHHHHHHCHHHHHHHHHCCCCCEEECCCEEEECCCHHHHHHHCCCCCCEEEEECCCH
LYQIAKRFNVQLTNLKTWNKGADVLRPGQLLTLYLPN
HHHHHHHHCEEEEEEECCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]