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The map label for this gene is livM [H]

Identifier: 145301112

GI number: 145301112

Start: 4580817

End: 4582085

Strand: Direct

Name: livM [H]

Synonym: ASA_4281

Alternate gene names: 145301112

Gene position: 4580817-4582085 (Clockwise)

Preceding gene: 145301111

Following gene: 145301113

Centisome position: 97.41

GC content: 59.26

Gene sequence:

>1269_bases
ATGAAACATCAATTTATTCATGCCTGCATCGCCAGTCTGGTGCTGATGGTGCTCTCCTTCTGGTTGCTTGGTTTTCGGCT
CGAGACGGAAGGCAGCCAGTTGCTGGTCGTGAGCCGCCTCGATATATCGCTGGCCTGGCTGGTGGGTGGCGCGGCACTGG
TGTTCTGGTTCCAGCTGTTACGTGGCCAGATCGGCAGATTGTTTCAGGCCTCCATCAGCGGCTTCTCCACCGGTTTCACC
AAGCTGGTGCTGCCAAGCCCGGAAGAGGCGCCACGGCTCTACAATGTCACCGCCATTCTGGTGCTGCTGTTTGCGGTGAG
CTGGCCCTTCATGGCCTCTCGCGGTGCCATCGATCTGGCAACCCTGGCGCTGATTTACATCATGCTGGGGCTGGGCCTCA
ACGTGGTGGTCGGTCTGGCTGGCCTGCTGGATCTGGGCTATGTGGGCTTTTACGCAGTCGGCGCTTACAGCTATGCCTTG
CTCAACACCTATTTCGGCCTGAGCTTCTGGGAGTGCCTGCCCATAGCGGGACTGATGGCCGCCACCTTCGGCTTTTTGCT
CGGCTTTCCGGTATTGCGGCTACGGGGGGACTATCTGGCCATCGTCACCCTGGGGTTTGGCGAGATCATCCGCATCCTGC
TCAACAACATGACCAGCCTCACCGGCGGCCCGAACGGCATCTCCGGCATTCCCAAGCCGACCCTGGCTGGACTGGAATTC
AACCGTACCGTCAAAGATGGGGGCTTTGGTACCTTCCACGACTTCTTTGGCATCGCCTACAACGCCAACCACAAGGTGAT
CTTCCTCTATCTGATGGCACTGGTGCTGGTTGTCGCCACCCTGTTCGTCATCAATCGCCTGCTGCGCATGCCGCTGGGTC
GAGCCTGGGAAGCGCTGCGGGAAGACGAAATCGCCTGCAAGTCCCTGGGGCTGAACCCCACCATCATCAAGCTGACCGCC
TTCACCATAGGTGCCACCTTTGCGGGGTTCGCCGGCAGTTTCTTCGCCTCCCGTCAGGGGTTTATCAGCCCTGAATCCTT
CGTCTTCATCGAGTCGGCCATAGTGCTCGCCATCGTCGTGCTGGGCGGCATGGGCTCCCAGATAGGGGTGGTGCTGGCGG
CCATCGTCATGACTGTGCTGCCGGAGCTTGCCCGTGAATTCAACGAGTACCGCATGCTGATGTTCGGCCTCTTGATGGTG
TTCATGATGATCTGGCGGCCACAAGGACTGCTGCCGATGACCAGACCTCACCTGGAGCTACAAAAATGA

Upstream 100 bases:

>100_bases
TCAGCACCGAATACAAGGATGTGATGGCCTTCGTCCTGCTGATCGGTGTCTTGCTGTTCATGCCCACCGGCATTCTGGGT
CGTCCGGAGGTCGAAAAAGT

Downstream 100 bases:

>100_bases
GCGCGAATCAGAAGAAGTTATTGGAAGTCTCTGATCTGTCGATGCGCTTTGGTGGACTGCTCGCAGTCAACGGCGTCAAG
CTGGATGTCGACAAAGGGGA

Product: leucine/isoleucine/valine transporter permease subunit

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKHQFIHACIASLVLMVLSFWLLGFRLETEGSQLLVVSRLDISLAWLVGGAALVFWFQLLRGQIGRLFQASISGFSTGFT
KLVLPSPEEAPRLYNVTAILVLLFAVSWPFMASRGAIDLATLALIYIMLGLGLNVVVGLAGLLDLGYVGFYAVGAYSYAL
LNTYFGLSFWECLPIAGLMAATFGFLLGFPVLRLRGDYLAIVTLGFGEIIRILLNNMTSLTGGPNGISGIPKPTLAGLEF
NRTVKDGGFGTFHDFFGIAYNANHKVIFLYLMALVLVVATLFVINRLLRMPLGRAWEALREDEIACKSLGLNPTIIKLTA
FTIGATFAGFAGSFFASRQGFISPESFVFIESAIVLAIVVLGGMGSQIGVVLAAIVMTVLPELAREFNEYRMLMFGLLMV
FMMIWRPQGLLPMTRPHLELQK

Sequences:

>Translated_422_residues
MKHQFIHACIASLVLMVLSFWLLGFRLETEGSQLLVVSRLDISLAWLVGGAALVFWFQLLRGQIGRLFQASISGFSTGFT
KLVLPSPEEAPRLYNVTAILVLLFAVSWPFMASRGAIDLATLALIYIMLGLGLNVVVGLAGLLDLGYVGFYAVGAYSYAL
LNTYFGLSFWECLPIAGLMAATFGFLLGFPVLRLRGDYLAIVTLGFGEIIRILLNNMTSLTGGPNGISGIPKPTLAGLEF
NRTVKDGGFGTFHDFFGIAYNANHKVIFLYLMALVLVVATLFVINRLLRMPLGRAWEALREDEIACKSLGLNPTIIKLTA
FTIGATFAGFAGSFFASRQGFISPESFVFIESAIVLAIVVLGGMGSQIGVVLAAIVMTVLPELAREFNEYRMLMFGLLMV
FMMIWRPQGLLPMTRPHLELQK
>Mature_422_residues
MKHQFIHACIASLVLMVLSFWLLGFRLETEGSQLLVVSRLDISLAWLVGGAALVFWFQLLRGQIGRLFQASISGFSTGFT
KLVLPSPEEAPRLYNVTAILVLLFAVSWPFMASRGAIDLATLALIYIMLGLGLNVVVGLAGLLDLGYVGFYAVGAYSYAL
LNTYFGLSFWECLPIAGLMAATFGFLLGFPVLRLRGDYLAIVTLGFGEIIRILLNNMTSLTGGPNGISGIPKPTLAGLEF
NRTVKDGGFGTFHDFFGIAYNANHKVIFLYLMALVLVVATLFVINRLLRMPLGRAWEALREDEIACKSLGLNPTIIKLTA
FTIGATFAGFAGSFFASRQGFISPESFVFIESAIVLAIVVLGGMGSQIGVVLAAIVMTVLPELAREFNEYRMLMFGLLMV
FMMIWRPQGLLPMTRPHLELQK

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=415, Percent_Identity=64.578313253012, Blast_Score=489, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 46125; Mature: 46125

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHQFIHACIASLVLMVLSFWLLGFRLETEGSQLLVVSRLDISLAWLVGGAALVFWFQLL
CCCHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHH
RGQIGRLFQASISGFSTGFTKLVLPSPEEAPRLYNVTAILVLLFAVSWPFMASRGAIDLA
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCHHHH
TLALIYIMLGLGLNVVVGLAGLLDLGYVGFYAVGAYSYALLNTYFGLSFWECLPIAGLMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATFGFLLGFPVLRLRGDYLAIVTLGFGEIIRILLNNMTSLTGGPNGISGIPKPTLAGLEF
HHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
NRTVKDGGFGTFHDFFGIAYNANHKVIFLYLMALVLVVATLFVINRLLRMPLGRAWEALR
CCEECCCCCCHHHHHHCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EDEIACKSLGLNPTIIKLTAFTIGATFAGFAGSFFASRQGFISPESFVFIESAIVLAIVV
HHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LGGMGSQIGVVLAAIVMTVLPELAREFNEYRMLMFGLLMVFMMIWRPQGLLPMTRPHLEL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
QK
CC
>Mature Secondary Structure
MKHQFIHACIASLVLMVLSFWLLGFRLETEGSQLLVVSRLDISLAWLVGGAALVFWFQLL
CCCHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHH
RGQIGRLFQASISGFSTGFTKLVLPSPEEAPRLYNVTAILVLLFAVSWPFMASRGAIDLA
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCHHHH
TLALIYIMLGLGLNVVVGLAGLLDLGYVGFYAVGAYSYALLNTYFGLSFWECLPIAGLMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATFGFLLGFPVLRLRGDYLAIVTLGFGEIIRILLNNMTSLTGGPNGISGIPKPTLAGLEF
HHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
NRTVKDGGFGTFHDFFGIAYNANHKVIFLYLMALVLVVATLFVINRLLRMPLGRAWEALR
CCEECCCCCCHHHHHHCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EDEIACKSLGLNPTIIKLTAFTIGATFAGFAGSFFASRQGFISPESFVFIESAIVLAIVV
HHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LGGMGSQIGVVLAAIVMTVLPELAREFNEYRMLMFGLLMVFMMIWRPQGLLPMTRPHLEL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
QK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]