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The map label for this gene is aglB [H]

Identifier: 145301108

GI number: 145301108

Start: 4575864

End: 4577285

Strand: Direct

Name: aglB [H]

Synonym: ASA_4277

Alternate gene names: 145301108

Gene position: 4575864-4577285 (Clockwise)

Preceding gene: 145301107

Following gene: 145301110

Centisome position: 97.31

GC content: 60.83

Gene sequence:

>1422_bases
ATGATCATGAAAAAATTCTCTGTAGTCATCGCCGGCGGCGGCAGCACTTTCACACCGGGTATCGTCCTCATGCTGCTCGA
GAACCAGCATCGTTTCCCGCTCAAGGAGATCAAGTTCTACGACAACGACGGTGCCCGCCAGGAAGTCATCGCCGAGGCAT
GCAGGATCCTGCTCAAGGAGAAGGCACCCGAGATTGCCTTCAGCTACGGTACCGATCCCAAGGCCGCATTCAGCGAAGTG
GATTTCGTCATGGCCCACATCCGGGTCGGCAAATACCCCATGCGCGAGCTGGACGAGAAGATCCCCCTGCGTCACGGCGT
GGTCGGTCAGGAGACCTGCGGCCCGGGCGGCATCGCCTACGGCATGCGCTCCATAGGTGGCGTGCTGGAGCTGGTCGACT
ATATGGAGCAGTACTCCCCCAACGCCTGGATGCTGAACTACTCCAACCCGGCCGCCATAGTGGCGGAGGCGACCCGTCGC
CTGCGCCCCAACGCCAAGATCCTGAACATTTGTGATATGCCGATCGGGATCGAGGGCCGCATGGCCCAGATCGCCGGCCT
TAAATCCCGTAAAGAGATGCGGGTGCGCTACTACGGCCTCAACCACTTCGGCTGGTGGACCCGCATCGAGGATCTGGCAG
GCAACGACCTGATGCCCAAGATCAAGGAGCACGTCGCCCGCCACGGCTATGTGCCCGATCACGCCAGCCATGCCGAGGCA
AGCTGGAACGATACCTTCGCCAAGGCCAGGGATGTGTGGGCACTCGATCCGGATACCCTGCCCAACACCTACCTCAAGTA
CTATCTCTTTCCGGACTATGTGGTGGCCCATGCCAACCCGGAACATACCCGCGCCAACGAGGTGATGGAGCACCGTGAGA
AACAGGTGTTCAGCGCCTGCCGCGCCATCGTCAAGGCGGGTCACTCAGAAGCGGGCGAGCTGGAGATCGACGAGCACGCC
TCTTATATAGTGGATCTGGCCACCGCCATCGCCTTCAACACCCAGGAGCGGATGCTGCTGATAGTCCCCAACCGGGGTGC
CATCAACAACTTCGACCCCGAGGCCATGGTCGAAATCCCCTGCCTGGTGGGCAGCAGCGGGCCCGAGCCCTTGCAGGTCG
GCGATATCCCCCTGTTCCAGCAAGGCTTGATGGGCCAGCAGGTGGCCGTCGAGAAGCTGGTGGTGGAAGCCTGGGTCGAG
CGCTCCTACCAGAAGCTGTGGCAGGCGATCACCTTGTCCAAGACGGTGCGAGTGCCTCGGTGGCGAAGGCCATCCTGGAT
GACCTGGTGGCCGCCAACAAGGATTTCTGGCCAGATCTGCAATAAACCGCCCATAAACAAAAACCCCCTCGGTAAAGAGG
GGGTTTTTCGTTGTGCCGACTATGCCGTGCAATACGGCATGGCGGTCAGCGCAGGGATTTGA

Upstream 100 bases:

>100_bases
GCATTCAAATAATCATAGGTCTGCATGTGCCACAGGTACGTGAAAATATGGAATCGATAATGAACAACCCCACTCAATCC
TCTTCTCTCGTAACGGAGTC

Downstream 100 bases:

>100_bases
CCGAGCGACGCACGATAAGCTCGGGCTCTACCGTCATGGACTCGACTTCCAGCGCCTTGTTGCGGATCCGGCTCACCAGA
TGCTTCACCGCCAGCAGGCC

Product: 6-phospho-alpha-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MIMKKFSVVIAGGGSTFTPGIVLMLLENQHRFPLKEIKFYDNDGARQEVIAEACRILLKEKAPEIAFSYGTDPKAAFSEV
DFVMAHIRVGKYPMRELDEKIPLRHGVVGQETCGPGGIAYGMRSIGGVLELVDYMEQYSPNAWMLNYSNPAAIVAEATRR
LRPNAKILNICDMPIGIEGRMAQIAGLKSRKEMRVRYYGLNHFGWWTRIEDLAGNDLMPKIKEHVARHGYVPDHASHAEA
SWNDTFAKARDVWALDPDTLPNTYLKYYLFPDYVVAHANPEHTRANEVMEHREKQVFSACRAIVKAGHSEAGELEIDEHA
SYIVDLATAIAFNTQERMLLIVPNRGAINNFDPEAMVEIPCLVGSSGPEPLQVGDIPLFQQGLMGQQVAVEKLVVEAWVE
RSYQKLWQAITLSKTVRVPRWRRPSWMTWWPPTRISGQICNKPPINKNPLGKEGVFRCADYAVQYGMAVSAGI

Sequences:

>Translated_473_residues
MIMKKFSVVIAGGGSTFTPGIVLMLLENQHRFPLKEIKFYDNDGARQEVIAEACRILLKEKAPEIAFSYGTDPKAAFSEV
DFVMAHIRVGKYPMRELDEKIPLRHGVVGQETCGPGGIAYGMRSIGGVLELVDYMEQYSPNAWMLNYSNPAAIVAEATRR
LRPNAKILNICDMPIGIEGRMAQIAGLKSRKEMRVRYYGLNHFGWWTRIEDLAGNDLMPKIKEHVARHGYVPDHASHAEA
SWNDTFAKARDVWALDPDTLPNTYLKYYLFPDYVVAHANPEHTRANEVMEHREKQVFSACRAIVKAGHSEAGELEIDEHA
SYIVDLATAIAFNTQERMLLIVPNRGAINNFDPEAMVEIPCLVGSSGPEPLQVGDIPLFQQGLMGQQVAVEKLVVEAWVE
RSYQKLWQAITLSKTVRVPRWRRPSWMTWWPPTRISGQICNKPPINKNPLGKEGVFRCADYAVQYGMAVSAGI
>Mature_473_residues
MIMKKFSVVIAGGGSTFTPGIVLMLLENQHRFPLKEIKFYDNDGARQEVIAEACRILLKEKAPEIAFSYGTDPKAAFSEV
DFVMAHIRVGKYPMRELDEKIPLRHGVVGQETCGPGGIAYGMRSIGGVLELVDYMEQYSPNAWMLNYSNPAAIVAEATRR
LRPNAKILNICDMPIGIEGRMAQIAGLKSRKEMRVRYYGLNHFGWWTRIEDLAGNDLMPKIKEHVARHGYVPDHASHAEA
SWNDTFAKARDVWALDPDTLPNTYLKYYLFPDYVVAHANPEHTRANEVMEHREKQVFSACRAIVKAGHSEAGELEIDEHA
SYIVDLATAIAFNTQERMLLIVPNRGAINNFDPEAMVEIPCLVGSSGPEPLQVGDIPLFQQGLMGQQVAVEKLVVEAWVE
RSYQKLWQAITLSKTVRVPRWRRPSWMTWWPPTRISGQICNKPPINKNPLGKEGVFRCADYAVQYGMAVSAGI

Specific function: Is involved in the catabolism of alpha-glycosides accumulated via a phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). Hydrolyzes a wide variety of 6-phospho-alpha-D- glucosides including maltose-6'-phosphate, isomaltose-6'- phosphate, mal

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=377, Percent_Identity=28.3819628647215, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1790560, Length=427, Percent_Identity=22.7166276346604, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.122 [H]

Molecular weight: Translated: 53171; Mature: 53171

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMKKFSVVIAGGGSTFTPGIVLMLLENQHRFPLKEIKFYDNDGARQEVIAEACRILLKE
CCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHEEEECCCCHHHHHHHHHHHHHHHH
KAPEIAFSYGTDPKAAFSEVDFVMAHIRVGKYPMRELDEKIPLRHGVVGQETCGPGGIAY
CCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHCCCCCCCCCCCCHHHH
GMRSIGGVLELVDYMEQYSPNAWMLNYSNPAAIVAEATRRLRPNAKILNICDMPIGIEGR
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
MAQIAGLKSRKEMRVRYYGLNHFGWWTRIEDLAGNDLMPKIKEHVARHGYVPDHASHAEA
HHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCC
SWNDTFAKARDVWALDPDTLPNTYLKYYLFPDYVVAHANPEHTRANEVMEHREKQVFSAC
CCCHHHHHHCCCEECCCCCCCCHHEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
RAIVKAGHSEAGELEIDEHASYIVDLATAIAFNTQERMLLIVPNRGAINNFDPEAMVEIP
HHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEEEE
CLVGSSGPEPLQVGDIPLFQQGLMGQQVAVEKLVVEAWVERSYQKLWQAITLSKTVRVPR
EEECCCCCCCCEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
WRRPSWMTWWPPTRISGQICNKPPINKNPLGKEGVFRCADYAVQYGMAVSAGI
CCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure
MIMKKFSVVIAGGGSTFTPGIVLMLLENQHRFPLKEIKFYDNDGARQEVIAEACRILLKE
CCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHEEEECCCCHHHHHHHHHHHHHHHH
KAPEIAFSYGTDPKAAFSEVDFVMAHIRVGKYPMRELDEKIPLRHGVVGQETCGPGGIAY
CCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHCCCCCCCCCCCCHHHH
GMRSIGGVLELVDYMEQYSPNAWMLNYSNPAAIVAEATRRLRPNAKILNICDMPIGIEGR
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
MAQIAGLKSRKEMRVRYYGLNHFGWWTRIEDLAGNDLMPKIKEHVARHGYVPDHASHAEA
HHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCC
SWNDTFAKARDVWALDPDTLPNTYLKYYLFPDYVVAHANPEHTRANEVMEHREKQVFSAC
CCCHHHHHHCCCEECCCCCCCCHHEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
RAIVKAGHSEAGELEIDEHASYIVDLATAIAFNTQERMLLIVPNRGAINNFDPEAMVEIP
HHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEEEE
CLVGSSGPEPLQVGDIPLFQQGLMGQQVAVEKLVVEAWVERSYQKLWQAITLSKTVRVPR
EEECCCCCCCCEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
WRRPSWMTWWPPTRISGQICNKPPINKNPLGKEGVFRCADYAVQYGMAVSAGI
CCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11473129; 11322729 [H]