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The map label for this gene is rsmB [H]

Identifier: 145300988

GI number: 145300988

Start: 4432016

End: 4433308

Strand: Direct

Name: rsmB [H]

Synonym: ASA_4142

Alternate gene names: 145300988

Gene position: 4432016-4433308 (Clockwise)

Preceding gene: 145300987

Following gene: 145300989

Centisome position: 94.25

GC content: 63.42

Gene sequence:

>1293_bases
ATGAAAACACGTGCACAGGCCGCTCTCGTTATTCAACAGGTGCTGGATCAGGGCCAATCCCTCTCCGCTGTTCTGCCTGC
TGCCCAGGAGAAGGTTGCGCCCCGCGATCGCGCCCTCCTGCAAGAACTCTGCTATGGCACCCTGCGCTGGCTGCCCCGCC
TCGACGCTGCGGTGGGCGAGATGATGGACAAGCCGCTCAAGAACAAGAGCCGCATCTTCCACTATCTGATCCTGGTCGGC
CTCTATCAGCTCATCTACACCCGCATTCCGGCCCACGCTGCCGTGGCCGAGACAGTCAACGCCGTCAAGCTGCTCAAGGG
TACCTCCTTGCGCGGCCTCATCAACGGTGTGCTGCGCAACTTCCAGCGCAGCGCCGAAGTCATATTGCTGCGCATCGACC
GAGTCCCCAGTATTCGCCTCGGTCACCCGGAGTGGCTCACCAAGCGCCTGCGTCAGGCCTATCCCGAAGATTGGGAATTC
ATCATGGAGGCCAACAATCAGCGCCCCCCCATGTGGATCCGCAACAATAGCCAGCGCCAGGGCCGCGAGCAGATGCTGGC
TCGCATGGCCGAGGTCGGCATCAATGCGGTTGCCGGTCAGGAGGGCGAAGATTGCATTCTGCTGGAACGTCCCTGCGACG
TGACCAAGCTGCCCGGTTTCGAGACAGGTGACTGCTCGGTGCAAGACGGTGCTGCCCAACAGGCCGCCCGACTGCTCGAT
CCGCAGCCGGGTGAGTGGGTGCTGGATGCCTGTGCCGCTCCCGGTGGCAAGACGGCCCACCTGCTTGAGCGTCAACCCGC
GCTGGCTGGCGTAGTGGCCGTGGATGCCGACGAGAACCGCCTCAAGCGGGTGCAGGAAAACCTGGATCGTATCGGCCTCC
AGGCCCGGGTGATCCATGGCGATGCCAGCACCCCTGAGCTGTGGTGGCCACAAGGCCAGTTCGACCGCATCCTGCTGGAC
GCCCCCTGCTCCGCCACCGGTGTCATCCGTCGTCACCCCGACATCAAGTGGTTGCGCCGGGATCAGGACATCCGCGAGCT
GGCCGAGTTGCAGCGCCGTATTCTGATCGCCCTCTGGTCGAAACTGAAAAGCGGCGGTACTCTGCTCTACGCTACCTGCT
CAGTGCTGCCGGAAGAGAACCGCGAACAGATCCGCGCCTTCCTGGCCACTACGCCGGATGCAACCCTGGTGCCGCTGCAC
GCGCAGGACACACCAGATTGTCCGGGCCGCCAGTTCCTGCCAGGCGAAGCCGAGATGGATGGCTTCTACTACGCCAAGCT
GATCAAGCGATAG

Upstream 100 bases:

>100_bases
GGATCTGCTCAACTCTCGCCGCGACTGGTTTGAACCCGGCACGCAATTGAATTGAAACAAGCGGGGCCCAGGCCCCGACT
TGCGCATCTAAGGTAACACT

Downstream 100 bases:

>100_bases
GCAGAGCAGTGACCATGATCATGAGGGGTGCTGGCTGACAGCCCCCATTACTGCGCAACATCGAGCACGTTTTCCCTATG
AAAATCACCATGTTGATGAC

Product: ribosomal RNA small subunit methyltransferase B

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKTRAQAALVIQQVLDQGQSLSAVLPAAQEKVAPRDRALLQELCYGTLRWLPRLDAAVGEMMDKPLKNKSRIFHYLILVG
LYQLIYTRIPAHAAVAETVNAVKLLKGTSLRGLINGVLRNFQRSAEVILLRIDRVPSIRLGHPEWLTKRLRQAYPEDWEF
IMEANNQRPPMWIRNNSQRQGREQMLARMAEVGINAVAGQEGEDCILLERPCDVTKLPGFETGDCSVQDGAAQQAARLLD
PQPGEWVLDACAAPGGKTAHLLERQPALAGVVAVDADENRLKRVQENLDRIGLQARVIHGDASTPELWWPQGQFDRILLD
APCSATGVIRRHPDIKWLRRDQDIRELAELQRRILIALWSKLKSGGTLLYATCSVLPEENREQIRAFLATTPDATLVPLH
AQDTPDCPGRQFLPGEAEMDGFYYAKLIKR

Sequences:

>Translated_430_residues
MKTRAQAALVIQQVLDQGQSLSAVLPAAQEKVAPRDRALLQELCYGTLRWLPRLDAAVGEMMDKPLKNKSRIFHYLILVG
LYQLIYTRIPAHAAVAETVNAVKLLKGTSLRGLINGVLRNFQRSAEVILLRIDRVPSIRLGHPEWLTKRLRQAYPEDWEF
IMEANNQRPPMWIRNNSQRQGREQMLARMAEVGINAVAGQEGEDCILLERPCDVTKLPGFETGDCSVQDGAAQQAARLLD
PQPGEWVLDACAAPGGKTAHLLERQPALAGVVAVDADENRLKRVQENLDRIGLQARVIHGDASTPELWWPQGQFDRILLD
APCSATGVIRRHPDIKWLRRDQDIRELAELQRRILIALWSKLKSGGTLLYATCSVLPEENREQIRAFLATTPDATLVPLH
AQDTPDCPGRQFLPGEAEMDGFYYAKLIKR
>Mature_430_residues
MKTRAQAALVIQQVLDQGQSLSAVLPAAQEKVAPRDRALLQELCYGTLRWLPRLDAAVGEMMDKPLKNKSRIFHYLILVG
LYQLIYTRIPAHAAVAETVNAVKLLKGTSLRGLINGVLRNFQRSAEVILLRIDRVPSIRLGHPEWLTKRLRQAYPEDWEF
IMEANNQRPPMWIRNNSQRQGREQMLARMAEVGINAVAGQEGEDCILLERPCDVTKLPGFETGDCSVQDGAAQQAARLLD
PQPGEWVLDACAAPGGKTAHLLERQPALAGVVAVDADENRLKRVQENLDRIGLQARVIHGDASTPELWWPQGQFDRILLD
APCSATGVIRRHPDIKWLRRDQDIRELAELQRRILIALWSKLKSGGTLLYATCSVLPEENREQIRAFLATTPDATLVPLH
AQDTPDCPGRQFLPGEAEMDGFYYAKLIKR

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=347, Percent_Identity=30.5475504322767, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI76150623, Length=347, Percent_Identity=30.5475504322767, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI32698918, Length=189, Percent_Identity=32.8042328042328, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI301336155, Length=212, Percent_Identity=31.1320754716981, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI39995082, Length=212, Percent_Identity=31.1320754716981, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI270288818, Length=193, Percent_Identity=32.6424870466321, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI23199998, Length=193, Percent_Identity=32.6424870466321, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI8922322, Length=193, Percent_Identity=32.6424870466321, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI270288816, Length=193, Percent_Identity=32.6424870466321, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI40316918, Length=216, Percent_Identity=28.2407407407407, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI2367212, Length=427, Percent_Identity=56.9086651053864, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI87081985, Length=208, Percent_Identity=31.25, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17536757, Length=334, Percent_Identity=29.940119760479, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25143471, Length=173, Percent_Identity=34.6820809248555, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI115534021, Length=170, Percent_Identity=31.1764705882353, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71998419, Length=171, Percent_Identity=30.9941520467836, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=313, Percent_Identity=28.7539936102236, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319447, Length=177, Percent_Identity=34.4632768361582, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI22024126, Length=341, Percent_Identity=27.5659824046921, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24668781, Length=198, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21356513, Length=198, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21355201, Length=211, Percent_Identity=33.175355450237, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19920494, Length=191, Percent_Identity=29.8429319371728, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356579, Length=220, Percent_Identity=30, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 48221; Mature: 48221

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRAQAALVIQQVLDQGQSLSAVLPAAQEKVAPRDRALLQELCYGTLRWLPRLDAAVGE
CCCHHHHHHHHHHHHHCCCCCHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MMDKPLKNKSRIFHYLILVGLYQLIYTRIPAHAAVAETVNAVKLLKGTSLRGLINGVLRN
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FQRSAEVILLRIDRVPSIRLGHPEWLTKRLRQAYPEDWEFIMEANNQRPPMWIRNNSQRQ
HHHCCEEEEEEECCCCCEECCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEECCCCHHH
GREQMLARMAEVGINAVAGQEGEDCILLERPCDVTKLPGFETGDCSVQDGAAQQAARLLD
HHHHHHHHHHHHCCHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
PQPGEWVLDACAAPGGKTAHLLERQPALAGVVAVDADENRLKRVQENLDRIGLQARVIHG
CCCCCHHHHHHCCCCCCHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEEC
DASTPELWWPQGQFDRILLDAPCSATGVIRRHPDIKWLRRDQDIRELAELQRRILIALWS
CCCCCCCCCCCCCCCEEEEECCCCCCCHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
KLKSGGTLLYATCSVLPEENREQIRAFLATTPDATLVPLHAQDTPDCPGRQFLPGEAEMD
HHCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
GFYYAKLIKR
HHHHHHHHCC
>Mature Secondary Structure
MKTRAQAALVIQQVLDQGQSLSAVLPAAQEKVAPRDRALLQELCYGTLRWLPRLDAAVGE
CCCHHHHHHHHHHHHHCCCCCHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MMDKPLKNKSRIFHYLILVGLYQLIYTRIPAHAAVAETVNAVKLLKGTSLRGLINGVLRN
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FQRSAEVILLRIDRVPSIRLGHPEWLTKRLRQAYPEDWEFIMEANNQRPPMWIRNNSQRQ
HHHCCEEEEEEECCCCCEECCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEECCCCHHH
GREQMLARMAEVGINAVAGQEGEDCILLERPCDVTKLPGFETGDCSVQDGAAQQAARLLD
HHHHHHHHHHHHCCHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
PQPGEWVLDACAAPGGKTAHLLERQPALAGVVAVDADENRLKRVQENLDRIGLQARVIHG
CCCCCHHHHHHCCCCCCHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEEC
DASTPELWWPQGQFDRILLDAPCSATGVIRRHPDIKWLRRDQDIRELAELQRRILIALWS
CCCCCCCCCCCCCCCEEEEECCCCCCCHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
KLKSGGTLLYATCSVLPEENREQIRAFLATTPDATLVPLHAQDTPDCPGRQFLPGEAEMD
HHCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
GFYYAKLIKR
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]