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The map label for this gene is fmt [H]

Identifier: 145300987

GI number: 145300987

Start: 4431026

End: 4431970

Strand: Direct

Name: fmt [H]

Synonym: ASA_4141

Alternate gene names: 145300987

Gene position: 4431026-4431970 (Clockwise)

Preceding gene: 145300986

Following gene: 145300988

Centisome position: 94.23

GC content: 60.85

Gene sequence:

>945_bases
TTGAATAAGCTGAAACTGATTTTTGCCGGTACTCCCGACTTCGCCGCCCGTCACCTGGCGGCGTTGTTGTCTTCCGACCA
CGAGGTCGTTGCCGTTTATACCCAGCCGGACAAGCCTGCTGGCCGTGGTCAGAAGCTCACCGCCAGCCCGGTCAAGGAAC
TGGCTCTGACCCACAATCTGCCGGTTTACCAACCAGCTTCCCTGCGCAAAGAAGAGGCGCAAGCCGAACTGGCTTCTCTG
GGCGCCGACTTGATGGTGGTAGTGGCCTACGGCTTGATCCTGCCCAAGGTGGTGCTCGATACCCCGCGTCTGGGCTGTAT
CAACGTGCATGGATCTTTGCTGCCGCGCTGGCGCGGTGCAGCCCCGATCCAGCGCTCCATCTGGGCGGGTGATACCGAAA
CCGGCGTGACCATCATGCAGATGGATGTGGGGCTGGATACTGGCGCCATGATCAGAAAAGTGACCTGTCCCATCGCCGCC
AACGAAACCTCCACCAGCCTGTATGACAAGCTGGCCGAGCTGGGCCCGCAGGCACTGGTCGACACCATCAATGCCATGGC
CGCCGGTGAAACCGCAGCCGAGGAGCAAGACGATGCGCTGGCCAACTATGCCGAGAAGCTCTCCAAAGAGGAGGCTCGCA
TCGACTGGTCGATGGAAGCCGTCGCCATAGATCGCTGCATCCGCGCCTTCAATCCCTGGCCCATCAGCTGGTTTGAGGTG
GCGGGACAGACTATCAAGGTGTGGCAGGCCGAGGTCATCAACAGCGACCATGGCCAACCTACCGGCACCCTGCTCAAGGC
CGACAAGCAGGGGATCGACATCGCTACTGGCCTGGGTGTCCTGCGCCTGCTGACCCTGCAACCACCCGGCAAGAAGGCCA
TGTCCGTTTCGGATCTGCTCAACTCTCGCCGCGACTGGTTTGAACCCGGCACGCAATTGAATTGA

Upstream 100 bases:

>100_bases
GTTTGTGGATTACTTGTCTCCGCTCAAGCGCCAGCGCATTCGCCAGAAGCTGGAAAAGATGGCCCGTGAAGATCGCAAAG
CCCTCTGAGGATCTGCCCCA

Downstream 100 bases:

>100_bases
AACAAGCGGGGCCCAGGCCCCGACTTGCGCATCTAAGGTAACACTATGAAAACACGTGCACAGGCCGCTCTCGTTATTCA
ACAGGTGCTGGATCAGGGCC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MNKLKLIFAGTPDFAARHLAALLSSDHEVVAVYTQPDKPAGRGQKLTASPVKELALTHNLPVYQPASLRKEEAQAELASL
GADLMVVVAYGLILPKVVLDTPRLGCINVHGSLLPRWRGAAPIQRSIWAGDTETGVTIMQMDVGLDTGAMIRKVTCPIAA
NETSTSLYDKLAELGPQALVDTINAMAAGETAAEEQDDALANYAEKLSKEEARIDWSMEAVAIDRCIRAFNPWPISWFEV
AGQTIKVWQAEVINSDHGQPTGTLLKADKQGIDIATGLGVLRLLTLQPPGKKAMSVSDLLNSRRDWFEPGTQLN

Sequences:

>Translated_314_residues
MNKLKLIFAGTPDFAARHLAALLSSDHEVVAVYTQPDKPAGRGQKLTASPVKELALTHNLPVYQPASLRKEEAQAELASL
GADLMVVVAYGLILPKVVLDTPRLGCINVHGSLLPRWRGAAPIQRSIWAGDTETGVTIMQMDVGLDTGAMIRKVTCPIAA
NETSTSLYDKLAELGPQALVDTINAMAAGETAAEEQDDALANYAEKLSKEEARIDWSMEAVAIDRCIRAFNPWPISWFEV
AGQTIKVWQAEVINSDHGQPTGTLLKADKQGIDIATGLGVLRLLTLQPPGKKAMSVSDLLNSRRDWFEPGTQLN
>Mature_314_residues
MNKLKLIFAGTPDFAARHLAALLSSDHEVVAVYTQPDKPAGRGQKLTASPVKELALTHNLPVYQPASLRKEEAQAELASL
GADLMVVVAYGLILPKVVLDTPRLGCINVHGSLLPRWRGAAPIQRSIWAGDTETGVTIMQMDVGLDTGAMIRKVTCPIAA
NETSTSLYDKLAELGPQALVDTINAMAAGETAAEEQDDALANYAEKLSKEEARIDWSMEAVAIDRCIRAFNPWPISWFEV
AGQTIKVWQAEVINSDHGQPTGTLLKADKQGIDIATGLGVLRLLTLQPPGKKAMSVSDLLNSRRDWFEPGTQLN

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI238814322, Length=280, Percent_Identity=30.7142857142857, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI21614513, Length=284, Percent_Identity=29.5774647887324, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI164663775, Length=235, Percent_Identity=31.063829787234, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1789683, Length=310, Percent_Identity=68.7096774193548, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI1788589, Length=298, Percent_Identity=28.1879194630872, Blast_Score=121, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI133930964, Length=291, Percent_Identity=27.8350515463918, Blast_Score=98, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6319458, Length=265, Percent_Identity=27.1698113207547, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550868, Length=302, Percent_Identity=29.8013245033113, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI28571984, Length=224, Percent_Identity=32.1428571428571, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585660, Length=219, Percent_Identity=30.1369863013699, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 33942; Mature: 33942

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLKLIFAGTPDFAARHLAALLSSDHEVVAVYTQPDKPAGRGQKLTASPVKELALTHNL
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCC
PVYQPASLRKEEAQAELASLGADLMVVVAYGLILPKVVLDTPRLGCINVHGSLLPRWRGA
CCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
APIQRSIWAGDTETGVTIMQMDVGLDTGAMIRKVTCPIAANETSTSLYDKLAELGPQALV
CCHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHEECCCCCCCCHHHHHHHHHHHCHHHHH
DTINAMAAGETAAEEQDDALANYAEKLSKEEARIDWSMEAVAIDRCIRAFNPWPISWFEV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHH
AGQTIKVWQAEVINSDHGQPTGTLLKADKQGIDIATGLGVLRLLTLQPPGKKAMSVSDLL
CCCCEEEHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHEECCCCCCHHHHHHHHH
NSRRDWFEPGTQLN
HHHHHHCCCCCCCC
>Mature Secondary Structure
MNKLKLIFAGTPDFAARHLAALLSSDHEVVAVYTQPDKPAGRGQKLTASPVKELALTHNL
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCC
PVYQPASLRKEEAQAELASLGADLMVVVAYGLILPKVVLDTPRLGCINVHGSLLPRWRGA
CCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
APIQRSIWAGDTETGVTIMQMDVGLDTGAMIRKVTCPIAANETSTSLYDKLAELGPQALV
CCHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHEECCCCCCCCHHHHHHHHHHHCHHHHH
DTINAMAAGETAAEEQDDALANYAEKLSKEEARIDWSMEAVAIDRCIRAFNPWPISWFEV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHH
AGQTIKVWQAEVINSDHGQPTGTLLKADKQGIDIATGLGVLRLLTLQPPGKKAMSVSDLL
CCCCEEEHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHEECCCCCCHHHHHHHHH
NSRRDWFEPGTQLN
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA