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The map label for this gene is def

Identifier: 145300986

GI number: 145300986

Start: 4430501

End: 4431013

Strand: Direct

Name: def

Synonym: ASA_4140

Alternate gene names: 145300986

Gene position: 4430501-4431013 (Clockwise)

Preceding gene: 145300976

Following gene: 145300987

Centisome position: 94.22

GC content: 55.36

Gene sequence:

>513_bases
ATGGCCATTCTAGATGTATTGCGTTTTCCCGATGAACGTCTGCGCACAGTTGCAGCCCCTGTCGAGACCTTTACACCCGA
GTTACAACACATTGTAGATGATATGTTCGAAACCATGTACGCGGAGGAAGGCATAGGCCTGGCCGCCACTCAGGTCGACA
TTCATCAGCGCATCATAGTGATCGATGTCTCTGAGAACCGTGAAGATCCTCTGGTATTGATCAACCCCGAGATCCTCGAG
CAAGCGGGCAGCACAGGCATCGAAGAGGGCTGCCTCTCGGTGCCCGACCACAGAGCTCTGGTGCCACGCGCCGAGTGGGT
CAAGGTACGTGCACTGGATCGCAACGGCCAGCCGTTCGAGCTGGAAGCGGACGATCTGCTCGCTATCTGCATACAGCATG
AGATGGATCATCTGATCGGCAAGCTGTTTGTGGATTACTTGTCTCCGCTCAAGCGCCAGCGCATTCGCCAGAAGCTGGAA
AAGATGGCCCGTGAAGATCGCAAAGCCCTCTGA

Upstream 100 bases:

>100_bases
CTGTTACTTCTGCGCGCAAAGTGTTGCTGTTTTTTGTCGGCGTAAATGACTAGAATTACGCCAATATTGACTGCATTTAT
ACGAAACTAAAGAAAAAACT

Downstream 100 bases:

>100_bases
GGATCTGCCCCATTGAATAAGCTGAAACTGATTTTTGCCGGTACTCCCGACTTCGCCGCCCGTCACCTGGCGGCGTTGTT
GTCTTCCGACCACGAGGTCG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MAILDVLRFPDERLRTVAAPVETFTPELQHIVDDMFETMYAEEGIGLAATQVDIHQRIIVIDVSENREDPLVLINPEILE
QAGSTGIEEGCLSVPDHRALVPRAEWVKVRALDRNGQPFELEADDLLAICIQHEMDHLIGKLFVDYLSPLKRQRIRQKLE
KMAREDRKAL

Sequences:

>Translated_170_residues
MAILDVLRFPDERLRTVAAPVETFTPELQHIVDDMFETMYAEEGIGLAATQVDIHQRIIVIDVSENREDPLVLINPEILE
QAGSTGIEEGCLSVPDHRALVPRAEWVKVRALDRNGQPFELEADDLLAICIQHEMDHLIGKLFVDYLSPLKRQRIRQKLE
KMAREDRKAL
>Mature_169_residues
AILDVLRFPDERLRTVAAPVETFTPELQHIVDDMFETMYAEEGIGLAATQVDIHQRIIVIDVSENREDPLVLINPEILEQ
AGSTGIEEGCLSVPDHRALVPRAEWVKVRALDRNGQPFELEADDLLAICIQHEMDHLIGKLFVDYLSPLKRQRIRQKLEK
MAREDRKAL

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=163, Percent_Identity=34.3558282208589, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1789682, Length=164, Percent_Identity=79.2682926829268, Blast_Score=271, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24645728, Length=138, Percent_Identity=32.6086956521739, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24645726, Length=141, Percent_Identity=34.0425531914894, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_AERS4 (A4ST57)

Other databases:

- EMBL:   CP000644
- RefSeq:   YP_001143827.1
- ProteinModelPortal:   A4ST57
- SMR:   A4ST57
- STRING:   A4ST57
- GeneID:   4997273
- GenomeReviews:   CP000644_GR
- KEGG:   asa:ASA_4140
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   MVIKKFT
- PhylomeDB:   A4ST57
- ProtClustDB:   PRK00150
- BioCyc:   ASAL382245:ASA_4140-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19433; Mature: 19301

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILDVLRFPDERLRTVAAPVETFTPELQHIVDDMFETMYAEEGIGLAATQVDIHQRIIV
CCHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHCEEEE
IDVSENREDPLVLINPEILEQAGSTGIEEGCLSVPDHRALVPRAEWVKVRALDRNGQPFE
EEECCCCCCCEEEECHHHHHHCCCCCCHHCCCCCCCCCCCCCCHHHEEEEEECCCCCCCE
LEADDLLAICIQHEMDHLIGKLFVDYLSPLKRQRIRQKLEKMAREDRKAL
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AILDVLRFPDERLRTVAAPVETFTPELQHIVDDMFETMYAEEGIGLAATQVDIHQRIIV
CHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHCEEEE
IDVSENREDPLVLINPEILEQAGSTGIEEGCLSVPDHRALVPRAEWVKVRALDRNGQPFE
EEECCCCCCCEEEECHHHHHHCCCCCCHHCCCCCCCCCCCCCCHHHEEEEEECCCCCCCE
LEADDLLAICIQHEMDHLIGKLFVDYLSPLKRQRIRQKLEKMAREDRKAL
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA