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The map label for this gene is chaA [H]

Identifier: 145300810

GI number: 145300810

Start: 4243609

End: 4244706

Strand: Direct

Name: chaA [H]

Synonym: ASA_3951

Alternate gene names: 145300810

Gene position: 4243609-4244706 (Clockwise)

Preceding gene: 145300807

Following gene: 145300821

Centisome position: 90.24

GC content: 59.65

Gene sequence:

>1098_bases
ATGAAGTCATTTATCCGGCAAGAGATTGCCCTGCCCGTTGGCCTGCTTACCCTGCTGTTCTTCAAGACCATAGGGAGCGA
GCTGCTGACAGAGGCGACTCCGCTCGGGATTTATCTGCCTGTCTCGGCGGCGCTGCTTGCCATCGTCACCTGGGCCATTT
TCTCTGTGGTGCGCCACTCGGATGCCCTCGCCATCAAGCTGGGTGAACCCTTCGGTACCCTTATCCTGACGCTCTCCGTC
ATCTCGCTGGAAGTGGTGATGATCTCCTCCGTCATGCTCACAGGCGACCCCAATCCCGTGATGGCCCGCGATACCATGTT
TGCAGTGGTGATGCTGGTCACCAATGGCCTGGTGGGCTTCACACTGCTGTTTGGCGGCTGGCGCTACCACACCCAGAGCT
TCAATCTGGATGGGGTCAAATCCTATCTGGTCGCCATCATACCGCTGGCCCTGCTCTGTCTGGTGCTGCCCAACTTCACC
CGGGGTGGCACCATTGGCAGCATGTCGAGCCCCATGTCCTGGATGCTGATCATCATCTCCATCCTGCTCTACGGTATCTT
CCTGCTGATCCAGACCCGCAGCCACAGCCACTTCTTCGTCGACAGCGACCACGAGGATTACGAGGAGCACCACGGCCCGC
TGCGCAGCAACGTCTATCACACCATACTGCTGCTCGCCTATCTGGTGGTGGTGATCCTGCTGGCCAAGACCCTGGCCGTG
CCCATCAACTTCGGCGTGAAGATCATGGCGGCGCCTCCTGCGCTGGGTGGCTTTATCGTGGCCTGTATCGTACTGGCCCC
CGAGGCAGTCGGTGCCATCAAGGCAGCCTTCAACAACCAGTTGCAACGGGCCATGAACCTCTATTTCGGCTCCGTGCTGG
CTACCATAGCCCTGACAGTACCGGCCGTGCTGTTCATCGGCGCCATGCTGGGCCAGGAGGTGCGCCTCGGCCTCTCCGCC
GCCGACATGGTGCTGCTCGTCACCACGCTTATGGTCTGCAAGGTCACCTTCAGCAGCGGCCGCACCAATGTGCTGCACGG
CGCTACCCACCTGGTGCTGTTCGTGGTTTATCTGTTCCTGATGTTTGAACACGAATAA

Upstream 100 bases:

>100_bases
ACAGCCTTTATCATTGTCGGGGTAGTCACCTACCGTGACCTTGCATCATTGCCTCCATACCCCGTCGGCTCATGCCGCCC
CGAGATGAGAACCATTGCCT

Downstream 100 bases:

>100_bases
CCCGCGCCAGCCAACACCAGCAACGACAAGACCGCCCGATGGGCGGTCTTTTGCTATGAACCGAATGAGGGCTTAGAGGC
TGGGCAGCACACCGGCCGGG

Product: calcium/proton antiporter

Products: Na (I) [Periplasm]; Ca (II) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKSFIRQEIALPVGLLTLLFFKTIGSELLTEATPLGIYLPVSAALLAIVTWAIFSVVRHSDALAIKLGEPFGTLILTLSV
ISLEVVMISSVMLTGDPNPVMARDTMFAVVMLVTNGLVGFTLLFGGWRYHTQSFNLDGVKSYLVAIIPLALLCLVLPNFT
RGGTIGSMSSPMSWMLIIISILLYGIFLLIQTRSHSHFFVDSDHEDYEEHHGPLRSNVYHTILLLAYLVVVILLAKTLAV
PINFGVKIMAAPPALGGFIVACIVLAPEAVGAIKAAFNNQLQRAMNLYFGSVLATIALTVPAVLFIGAMLGQEVRLGLSA
ADMVLLVTTLMVCKVTFSSGRTNVLHGATHLVLFVVYLFLMFEHE

Sequences:

>Translated_365_residues
MKSFIRQEIALPVGLLTLLFFKTIGSELLTEATPLGIYLPVSAALLAIVTWAIFSVVRHSDALAIKLGEPFGTLILTLSV
ISLEVVMISSVMLTGDPNPVMARDTMFAVVMLVTNGLVGFTLLFGGWRYHTQSFNLDGVKSYLVAIIPLALLCLVLPNFT
RGGTIGSMSSPMSWMLIIISILLYGIFLLIQTRSHSHFFVDSDHEDYEEHHGPLRSNVYHTILLLAYLVVVILLAKTLAV
PINFGVKIMAAPPALGGFIVACIVLAPEAVGAIKAAFNNQLQRAMNLYFGSVLATIALTVPAVLFIGAMLGQEVRLGLSA
ADMVLLVTTLMVCKVTFSSGRTNVLHGATHLVLFVVYLFLMFEHE
>Mature_365_residues
MKSFIRQEIALPVGLLTLLFFKTIGSELLTEATPLGIYLPVSAALLAIVTWAIFSVVRHSDALAIKLGEPFGTLILTLSV
ISLEVVMISSVMLTGDPNPVMARDTMFAVVMLVTNGLVGFTLLFGGWRYHTQSFNLDGVKSYLVAIIPLALLCLVLPNFT
RGGTIGSMSSPMSWMLIIISILLYGIFLLIQTRSHSHFFVDSDHEDYEEHHGPLRSNVYHTILLLAYLVVVILLAKTLAV
PINFGVKIMAAPPALGGFIVACIVLAPEAVGAIKAAFNNQLQRAMNLYFGSVLATIALTVPAVLFIGAMLGQEVRLGLSA
ADMVLLVTTLMVCKVTFSSGRTNVLHGATHLVLFVVYLFLMFEHE

Specific function: According to PubMed:8496184 the natural activity of this protein is as a calcium/proton antiporter, but it can act as a weak sodium/proton antiporter. The presence of a preferred substrate and normal pH cytoplasmic values might prevent significant sodium/

COG id: COG0387

COG function: function code P; Ca2+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium/potassium/calcium exchanger family [H]

Homologues:

Organism=Escherichia coli, GI1787468, Length=331, Percent_Identity=48.3383685800604, Blast_Score=293, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004713
- InterPro:   IPR004837 [H]

Pfam domain/function: PF01699 Na_Ca_ex [H]

EC number: NA

Molecular weight: Translated: 39592; Mature: 39592

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSFIRQEIALPVGLLTLLFFKTIGSELLTEATPLGIYLPVSAALLAIVTWAIFSVVRHS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCC
DALAIKLGEPFGTLILTLSVISLEVVMISSVMLTGDPNPVMARDTMFAVVMLVTNGLVGF
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHCCHHHH
TLLFGGWRYHTQSFNLDGVKSYLVAIIPLALLCLVLPNFTRGGTIGSMSSPMSWMLIIIS
HHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
ILLYGIFLLIQTRSHSHFFVDSDHEDYEEHHGPLRSNVYHTILLLAYLVVVILLAKTLAV
HHHHHHHHHHHCCCCCEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
PINFGVKIMAAPPALGGFIVACIVLAPEAVGAIKAAFNNQLQRAMNLYFGSVLATIALTV
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PAVLFIGAMLGQEVRLGLSAADMVLLVTTLMVCKVTFSSGRTNVLHGATHLVLFVVYLFL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
MFEHE
HHCCC
>Mature Secondary Structure
MKSFIRQEIALPVGLLTLLFFKTIGSELLTEATPLGIYLPVSAALLAIVTWAIFSVVRHS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCC
DALAIKLGEPFGTLILTLSVISLEVVMISSVMLTGDPNPVMARDTMFAVVMLVTNGLVGF
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHCCHHHH
TLLFGGWRYHTQSFNLDGVKSYLVAIIPLALLCLVLPNFTRGGTIGSMSSPMSWMLIIIS
HHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
ILLYGIFLLIQTRSHSHFFVDSDHEDYEEHHGPLRSNVYHTILLLAYLVVVILLAKTLAV
HHHHHHHHHHHCCCCCEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
PINFGVKIMAAPPALGGFIVACIVLAPEAVGAIKAAFNNQLQRAMNLYFGSVLATIALTV
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PAVLFIGAMLGQEVRLGLSAADMVLLVTTLMVCKVTFSSGRTNVLHGATHLVLFVVYLFL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
MFEHE
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm]; Ca (II) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + Na (I) [Periplasm] Ca (II) [Cytoplasm] + Proton [Periplasm] = Ca (II) [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8496184; 8905232; 9278503 [H]