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The map label for this gene is rsmD [H]

Identifier: 145300821

GI number: 145300821

Start: 4256887

End: 4257516

Strand: Direct

Name: rsmD [H]

Synonym: ASA_3962

Alternate gene names: 145300821

Gene position: 4256887-4257516 (Clockwise)

Preceding gene: 145300810

Following gene: 145300822

Centisome position: 90.53

GC content: 61.43

Gene sequence:

>630_bases
ATGCCCAGAGTGAAACCCAGCCGTAACATCCCGAACAAGAGCCCGGCCCCGCAGGGGAAATCGGGCTTTGTAAGAATCAT
CAGCGGCCAGTGGAAGGGCCGCAAATTGCCGGTCAAGGATGTAGAAGGGCTCAGACCCACCACGGATCGCGTCAAGGAGA
CCATCTTCAACTGGCTGGCTCCCCATGTGCGTGGCGTTCGCTGTCTCGATCTGTTTGCCGGCAGCGGCGGCCTGGGGCTG
GAGGCGCTGTCGCGCTATGCCGAGCAGGTAACTCTGGTCGAGATGGACAAAAGTGCCGCCGACCAGATCAGAAAGAACCT
GACCGCCCTTGGCAGCAGCAAGGGCCTGGTGATCCAGTCCGATGCCGTCAGTTGGCTGCAAGGCCCGGCCACCCCGTTCG
ACCTGGTGTTCCTGGATCCGCCGTTTCGGCGCGAGCTGCTGCCCCGGGTGTGCGATTTGCTGGAACAGCACGGCTGGCTG
GCACCGGATGCTCTGATCTATCTGGAGCGGGAGAAAGAGATGGCCGACCTGGCGTTGCCCGCCAGCTGGCAACTGCTCAA
GGACAAGCAGGCCGGTCAAGTCTGCTATCAACTCTATCTGCGGGACACCAAGAATGACGCTGCGGTTTAA

Upstream 100 bases:

>100_bases
TCACTTCGTTGGCTGACTGCTAAGATAGCCGTCCCTCGGCGGGTCGGCATAAAAATGAACCGCCTTATACTACCACTTTA
TTTTCTGACGACGAAACGCG

Downstream 100 bases:

>100_bases
ATTCCGTGTAGGAAGGGGAACAAGCGTTGGCCCCTCCCCGTCAATTTAGGCAACGACTCAAGGGCAAGCAGGCCGGTCAG
GTCTGCTATCAACTCTATCT

Product: N6-adenine-specific methylase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MPRVKPSRNIPNKSPAPQGKSGFVRIISGQWKGRKLPVKDVEGLRPTTDRVKETIFNWLAPHVRGVRCLDLFAGSGGLGL
EALSRYAEQVTLVEMDKSAADQIRKNLTALGSSKGLVIQSDAVSWLQGPATPFDLVFLDPPFRRELLPRVCDLLEQHGWL
APDALIYLEREKEMADLALPASWQLLKDKQAGQVCYQLYLRDTKNDAAV

Sequences:

>Translated_209_residues
MPRVKPSRNIPNKSPAPQGKSGFVRIISGQWKGRKLPVKDVEGLRPTTDRVKETIFNWLAPHVRGVRCLDLFAGSGGLGL
EALSRYAEQVTLVEMDKSAADQIRKNLTALGSSKGLVIQSDAVSWLQGPATPFDLVFLDPPFRRELLPRVCDLLEQHGWL
APDALIYLEREKEMADLALPASWQLLKDKQAGQVCYQLYLRDTKNDAAV
>Mature_208_residues
PRVKPSRNIPNKSPAPQGKSGFVRIISGQWKGRKLPVKDVEGLRPTTDRVKETIFNWLAPHVRGVRCLDLFAGSGGLGLE
ALSRYAEQVTLVEMDKSAADQIRKNLTALGSSKGLVIQSDAVSWLQGPATPFDLVFLDPPFRRELLPRVCDLLEQHGWLA
PDALIYLEREKEMADLALPASWQLLKDKQAGQVCYQLYLRDTKNDAAV

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=195, Percent_Identity=54.8717948717949, Blast_Score=218, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 23274; Mature: 23143

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRVKPSRNIPNKSPAPQGKSGFVRIISGQWKGRKLPVKDVEGLRPTTDRVKETIFNWLA
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHH
PHVRGVRCLDLFAGSGGLGLEALSRYAEQVTLVEMDKSAADQIRKNLTALGSSKGLVIQS
HHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCEEEEC
DAVSWLQGPATPFDLVFLDPPFRRELLPRVCDLLEQHGWLAPDALIYLEREKEMADLALP
CCHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHCCC
ASWQLLKDKQAGQVCYQLYLRDTKNDAAV
CCHHHHHHCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PRVKPSRNIPNKSPAPQGKSGFVRIISGQWKGRKLPVKDVEGLRPTTDRVKETIFNWLA
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHH
PHVRGVRCLDLFAGSGGLGLEALSRYAEQVTLVEMDKSAADQIRKNLTALGSSKGLVIQS
HHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCEEEEC
DAVSWLQGPATPFDLVFLDPPFRRELLPRVCDLLEQHGWLAPDALIYLEREKEMADLALP
CCHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHCCC
ASWQLLKDKQAGQVCYQLYLRDTKNDAAV
CCHHHHHHCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]