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The map label for this gene is phoE [H]

Identifier: 145300742

GI number: 145300742

Start: 4167443

End: 4168504

Strand: Direct

Name: phoE [H]

Synonym: ASA_3881

Alternate gene names: 145300742

Gene position: 4167443-4168504 (Clockwise)

Preceding gene: 145300739

Following gene: 145300743

Centisome position: 88.62

GC content: 57.25

Gene sequence:

>1062_bases
ATGAAAAAGCTGACTGTAGTGGCACTGGCTCTCACCGCGGCCTTCCAGGCTCAGGCCGTTGAAGTGTACAAAAACGACAC
CACGATGGTGGATTTGTATGGACGTATCTACGCTGGCCATTTCTTCGGTGACAAGAAGGAAGGGGTCGACACTGCAAACC
CGAATGGCACTCATGACTACAGTGCCAAGCAGGGTGCAAACCAGTTTGTCCGCTTCGGCGCCAAGGTAGATAGCCAGATA
AACAGCCAACTGAAGGCGCTGGCCCAGTACGAAGTGCAGATGTACATCAATGACAGCGAGAAAACCATCAGCGACAACTC
GGACAACCTGCGTACCCGCCTGGCGTTCGCCGGTGTGGGCGCAGACTGGGGCAACATCACCTTCGGTCGCCAGAAGGGAG
CCCCCGGCTTCCTGGCCGACTGGACTGACGTCTCCCTCTCCGATGGCTATGGCAACGAAGCCCTCGGCGCCAAGACCGAC
ACCTTCGCCACCAACCGTGCCGGTTCAGTGCTGAAGTACTCGGGTCTGTTCAATGGCTTCCAGATAGACACCAGCTACAA
GTTTGATGGCGGTGATACCGAAGAGACCACCAGCAAGGACAGCGATGCCGCCTACGGTGCCGCCGTGGCTTACACCTTCC
CGTTCGACCTGGCACTGGGTACCGCCTACAACGTGGGGATCCGTGACAAGAGCGGTGAAGAAGATGCCAAGCTGTGGCTC
ATCTCCGCCAAATACGACAACAAGGCAGTCTATGCCGCCCTGAGCTATGCCGACGGCACCGACTTCCTGGCCACTGGCAC
CGACCACACAGGTTGGGAAGCTGCGCTCGGTTACAACTTCGAGAACGGTTTCGGCCTGATGGCACTGTGGAACAAGCAGG
AACAGGACAAGGCGGGTACCAAGAAAGACAATCTGGATTACTACACCCTGGGCGCCCAGTACAAGTTCAACAAGAACCTG
CGCGTCATCGGCGAGTACCGCATCAACAACCTGGACAGTGTTGCCGGTAAAGCAACCGCCAACAAGGATGACTTCCAGCT
GGCGGCCCGTTACGACTTCTAA

Upstream 100 bases:

>100_bases
AACCGCCATCGACACTTAACGGGATTGTCACCCCGCCGTCATTCCCCCTGCCTAGCATGGCCCCGTCTTTTAAAACAAAA
GCTTGCAACAAGGGATCAAG

Downstream 100 bases:

>100_bases
GTCGGGTTTTCAGCAAGAAAAACAACGCGCTGGCTTATGCCGGCGCGTTTTTTTGTGGCCCCCTGTCAACCCGGCATCCA
GCCATGACCTAGGCTCAACA

Product: outer membrane porin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKKLTVVALALTAAFQAQAVEVYKNDTTMVDLYGRIYAGHFFGDKKEGVDTANPNGTHDYSAKQGANQFVRFGAKVDSQI
NSQLKALAQYEVQMYINDSEKTISDNSDNLRTRLAFAGVGADWGNITFGRQKGAPGFLADWTDVSLSDGYGNEALGAKTD
TFATNRAGSVLKYSGLFNGFQIDTSYKFDGGDTEETTSKDSDAAYGAAVAYTFPFDLALGTAYNVGIRDKSGEEDAKLWL
ISAKYDNKAVYAALSYADGTDFLATGTDHTGWEAALGYNFENGFGLMALWNKQEQDKAGTKKDNLDYYTLGAQYKFNKNL
RVIGEYRINNLDSVAGKATANKDDFQLAARYDF

Sequences:

>Translated_353_residues
MKKLTVVALALTAAFQAQAVEVYKNDTTMVDLYGRIYAGHFFGDKKEGVDTANPNGTHDYSAKQGANQFVRFGAKVDSQI
NSQLKALAQYEVQMYINDSEKTISDNSDNLRTRLAFAGVGADWGNITFGRQKGAPGFLADWTDVSLSDGYGNEALGAKTD
TFATNRAGSVLKYSGLFNGFQIDTSYKFDGGDTEETTSKDSDAAYGAAVAYTFPFDLALGTAYNVGIRDKSGEEDAKLWL
ISAKYDNKAVYAALSYADGTDFLATGTDHTGWEAALGYNFENGFGLMALWNKQEQDKAGTKKDNLDYYTLGAQYKFNKNL
RVIGEYRINNLDSVAGKATANKDDFQLAARYDF
>Mature_353_residues
MKKLTVVALALTAAFQAQAVEVYKNDTTMVDLYGRIYAGHFFGDKKEGVDTANPNGTHDYSAKQGANQFVRFGAKVDSQI
NSQLKALAQYEVQMYINDSEKTISDNSDNLRTRLAFAGVGADWGNITFGRQKGAPGFLADWTDVSLSDGYGNEALGAKTD
TFATNRAGSVLKYSGLFNGFQIDTSYKFDGGDTEETTSKDSDAAYGAAVAYTFPFDLALGTAYNVGIRDKSGEEDAKLWL
ISAKYDNKAVYAALSYADGTDFLATGTDHTGWEAALGYNFENGFGLMALWNKQEQDKAGTKKDNLDYYTLGAQYKFNKNL
RVIGEYRINNLDSVAGKATANKDDFQLAARYDF

Specific function: Uptake of inorganic phosphate, phosphorylated compounds, and some other negatively charged solutes [H]

COG id: COG3203

COG function: function code M; Outer membrane protein (porin)

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Gram-negative porin family [H]

Homologues:

Organism=Escherichia coli, GI1786436, Length=369, Percent_Identity=28.7262872628726, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787160, Length=383, Percent_Identity=28.7206266318538, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1787641, Length=354, Percent_Identity=28.2485875706215, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788544, Length=368, Percent_Identity=29.0760869565217, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: 794 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001897
- InterPro:   IPR013793
- InterPro:   IPR001702 [H]

Pfam domain/function: PF00267 Porin_1 [H]

EC number: NA

Molecular weight: Translated: 38584; Mature: 38584

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTVVALALTAAFQAQAVEVYKNDTTMVDLYGRIYAGHFFGDKKEGVDTANPNGTHDY
CCCEEEEHHHHHHHHHHHEEEEECCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCC
SAKQGANQFVRFGAKVDSQINSQLKALAQYEVQMYINDSEKTISDNSDNLRTRLAFAGVG
CHHHCHHHHHHHCCHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCEEEEEEEEECC
ADWGNITFGRQKGAPGFLADWTDVSLSDGYGNEALGAKTDTFATNRAGSVLKYSGLFNGF
CCCCCEEECCCCCCCCCEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCE
QIDTSYKFDGGDTEETTSKDSDAAYGAAVAYTFPFDLALGTAYNVGIRDKSGEEDAKLWL
EEECEEEECCCCCCCCCCCCCCCCCCEEEEEEECCHHEECCEEEECCCCCCCCCCEEEEE
ISAKYDNKAVYAALSYADGTDFLATGTDHTGWEAALGYNFENGFGLMALWNKQEQDKAGT
EEECCCCCEEEEEEEECCCCCEEEECCCCCCCEEEEECCCCCCEEEEEEECCCCHHHCCC
KKDNLDYYTLGAQYKFNKNLRVIGEYRINNLDSVAGKATANKDDFQLAARYDF
CCCCCCEEEECCEEEECCCEEEEEEEECCCCHHHCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MKKLTVVALALTAAFQAQAVEVYKNDTTMVDLYGRIYAGHFFGDKKEGVDTANPNGTHDY
CCCEEEEHHHHHHHHHHHEEEEECCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCC
SAKQGANQFVRFGAKVDSQINSQLKALAQYEVQMYINDSEKTISDNSDNLRTRLAFAGVG
CHHHCHHHHHHHCCHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCEEEEEEEEECC
ADWGNITFGRQKGAPGFLADWTDVSLSDGYGNEALGAKTDTFATNRAGSVLKYSGLFNGF
CCCCCEEECCCCCCCCCEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCE
QIDTSYKFDGGDTEETTSKDSDAAYGAAVAYTFPFDLALGTAYNVGIRDKSGEEDAKLWL
EEECEEEECCCCCCCCCCCCCCCCCCEEEEEEECCHHEECCEEEECCCCCCCCCCEEEEE
ISAKYDNKAVYAALSYADGTDFLATGTDHTGWEAALGYNFENGFGLMALWNKQEQDKAGT
EEECCCCCEEEEEEEECCCCCEEEECCCCCCCEEEEECCCCCCEEEEEEECCCCHHHCCC
KKDNLDYYTLGAQYKFNKNLRVIGEYRINNLDSVAGKATANKDDFQLAARYDF
CCCCCCEEEECCEEEECCCEEEEEEEECCCCHHHCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1337052 [H]