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The map label for this gene is nudE [H]

Identifier: 145300657

GI number: 145300657

Start: 4082347

End: 4082955

Strand: Direct

Name: nudE [H]

Synonym: ASA_3789

Alternate gene names: 145300657

Gene position: 4082347-4082955 (Clockwise)

Preceding gene: 145300655

Following gene: 145300658

Centisome position: 86.81

GC content: 61.41

Gene sequence:

>609_bases
ATGAGTGACCACCACAAGAGCAAGCCCGAGCATATCTGTGCCGAGACCCTGGATCTCGACAAGAACAAGCCGGAGATCCT
CAAGGCCGAGATAGTGGCCGAGAGCCGGCTGTTCAAGGTGGAATCCCTGCACCTCAAATTCTCCAATGGCGAGGAGCGAG
TCTATGAGCGGATGCGGGGTGGCAACCGGGGCGCCGTCATGGTGGTGCCTATGTATGACGCTCACACCCTGCTGCTGGTG
CGCGAGTATGCGGCTGGTACTCACTCTTACGAGCTGGGCTTTCCCAAGGGGCTGATCGACCCGGGTGAGAACGCCTTCGA
GGCGGGCAACCGGGAGCTGATGGAGGAGGCCGGTTTTGGCGCCAACAGCCTGATCCCCCTCAAGCAGGTGTCACTGGCGC
CGGGTTACTTCTCCAGCCGGATGGACATACTGCTGGCGAGGGATCTCTTCCCCGAGCAGCGAGTCGGCGATGAACCCGAG
CCTCTGGAGGTGATCCCCTGGCCGCTCAACCGGATTGATGAATTGCTGGCGCGTTCCGACTTTACCGAGTCGCGCAGCAT
CAGTGCCCTGCTGCTGGCCCAGAAGTGGCTGGCGGAGCAGGAAAACTAG

Upstream 100 bases:

>100_bases
ACGGATTTTTCCATCCGTTTTCCCGCCCTTGCCCCGTGGTGTTCGGTGTGGGAATCGTTTACTCTTGAAGCACTATTTTT
TAACGAGCAGCCAACGCCCG

Downstream 100 bases:

>100_bases
CCCGAACGTGGGCAAAGGCAATAAAAACAGAGGATAACAAGATGCAGGAGTTGTTGGCCTGGGTTCCAGGGGTCAAGGAG
ATCGCCCGCGAAGCGGGACG

Product: ADP-ribose diphosphatase NudE

Products: AMP; D-Ribose 5- Phosphate. [C]

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MSDHHKSKPEHICAETLDLDKNKPEILKAEIVAESRLFKVESLHLKFSNGEERVYERMRGGNRGAVMVVPMYDAHTLLLV
REYAAGTHSYELGFPKGLIDPGENAFEAGNRELMEEAGFGANSLIPLKQVSLAPGYFSSRMDILLARDLFPEQRVGDEPE
PLEVIPWPLNRIDELLARSDFTESRSISALLLAQKWLAEQEN

Sequences:

>Translated_202_residues
MSDHHKSKPEHICAETLDLDKNKPEILKAEIVAESRLFKVESLHLKFSNGEERVYERMRGGNRGAVMVVPMYDAHTLLLV
REYAAGTHSYELGFPKGLIDPGENAFEAGNRELMEEAGFGANSLIPLKQVSLAPGYFSSRMDILLARDLFPEQRVGDEPE
PLEVIPWPLNRIDELLARSDFTESRSISALLLAQKWLAEQEN
>Mature_201_residues
SDHHKSKPEHICAETLDLDKNKPEILKAEIVAESRLFKVESLHLKFSNGEERVYERMRGGNRGAVMVVPMYDAHTLLLVR
EYAAGTHSYELGFPKGLIDPGENAFEAGNRELMEEAGFGANSLIPLKQVSLAPGYFSSRMDILLARDLFPEQRVGDEPEP
LEVIPWPLNRIDELLARSDFTESRSISALLLAQKWLAEQEN

Specific function: Active on adenosine(5')triphospho(5')adenosine (Ap3A), ADP-ribose, NADH, adenosine(5')diphospho(5')adenosine (Ap2A) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=179, Percent_Identity=61.4525139664804, Blast_Score=223, Evalue=9e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 22775; Mature: 22644

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHHKSKPEHICAETLDLDKNKPEILKAEIVAESRLFKVESLHLKFSNGEERVYERMRG
CCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHEEEEEEEEEECCCHHHHHHHHCC
GNRGAVMVVPMYDAHTLLLVREYAAGTHSYELGFPKGLIDPGENAFEAGNRELMEEAGFG
CCCCEEEEEEECCCCEEEEHHHHHCCCCEEECCCCCCCCCCCCHHHHCCCHHHHHHCCCC
ANSLIPLKQVSLAPGYFSSRMDILLARDLFPEQRVGDEPEPLEVIPWPLNRIDELLARSD
CCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCEEECCCHHHHHHHHHHCC
FTESRSISALLLAQKWLAEQEN
CCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDHHKSKPEHICAETLDLDKNKPEILKAEIVAESRLFKVESLHLKFSNGEERVYERMRG
CCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHEEEEEEEEEECCCHHHHHHHHCC
GNRGAVMVVPMYDAHTLLLVREYAAGTHSYELGFPKGLIDPGENAFEAGNRELMEEAGFG
CCCCEEEEEEECCCCEEEEHHHHHCCCCEEECCCCCCCCCCCCHHHHCCCHHHHHHCCCC
ANSLIPLKQVSLAPGYFSSRMDILLARDLFPEQRVGDEPEPLEVIPWPLNRIDELLARSD
CCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCEEECCCHHHHHHHHHHCC
FTESRSISALLLAQKWLAEQEN
CCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ADP-Ribose; H2O [C]

Specific reaction: ADP-Ribose + H2O = AMP + D-Ribose 5- Phosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9452430 [H]