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The map label for this gene is dapF [H]

Identifier: 145300548

GI number: 145300548

Start: 3967018

End: 3967848

Strand: Direct

Name: dapF [H]

Synonym: ASA_3680

Alternate gene names: 145300548

Gene position: 3967018-3967848 (Clockwise)

Preceding gene: 145300547

Following gene: 145300549

Centisome position: 84.36

GC content: 59.93

Gene sequence:

>831_bases
ATGTTGATAGATTTTTCCAAGATGCATGGGCTGGGTAACGACTTCATGGTGGTGGATGGTGTCACCCAGAAGGTGTTTTT
CAGCAATGACGTCATCAAGAAGCTGGCGGATCGCCACTTCGGGATCGGTTTTGACCAGCTGTTGCTGGTGGAGCCGCCTT
ATGACCCTGAACTCGACTTCCACTATCGCATCTTCAATGCCGATGGCAGCGAGGTGGAGCAGTGCGGCAATGGTGCCCGC
TGTTTCGCCCGTTTCGTGCGCCTCAAGGGGCTGATCAACCGGGATCGCATCGCGGTCAGCACGGCCCGTGGCCGCATTAG
CCTGCAGCTGGAAGGGGAGAATCAGGTCACGGTCAACATGGGGGTTCCCCAGTTTGAGCCGGGCAAGATCCCGTTTCGGG
CTCAGAAGGCGGAAAAAACCTATCTGTTGCGGGCACAAGAGCATACTGTGATGTGTGGTGTGGTCTCGATGGGCAACCCC
CACTGCGTCATTGAAGTTCCGTCGGTGGCTGATGCGCCGGTGGAGACTCTGGGTGCCATCATGGAGCGGCACGAGCGTTT
CCCCGAACGGGTCAACGTCGGCTTCATGGAGATGGTCAACGCCACCGAGATCCGCTTGCGGGTATTCGAGCGCGGTGTGG
GGGAGACCCTGGCCTGTGGTACCGGTGCCTGTGCGGCGGCGGTGATTGGCATCAGTCAGGGCAAGCTCAAGGAGCGGGTC
ACCGTCAGTCTGCCGGGCGGCAAGCTGACCATCGCCTGGAAGGGGCCGGGCCAGCCGGTCTATATGACGGGCCCGGCCGA
GCATGTATTCGACGGCCAGATAGAGTTATAG

Upstream 100 bases:

>100_bases
GTGTTGCGAGCATTGACGGCCCTGATAGCAGGGGAACGCACGATTGAGCGGATCGCTGGTGCGCCGAGCATTTACTGCCT
AAAGGGTACAGGAACGCACT

Downstream 100 bases:

>100_bases
GCATGACGAGGGCGCAAGCCCTCTCTGCTGCGCGATAATGTCAGGGCCCAGTGCCCTTTTTCTGTAATGGACCAACCAAT
TTATGAGCGAACTCAAACGG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MLIDFSKMHGLGNDFMVVDGVTQKVFFSNDVIKKLADRHFGIGFDQLLLVEPPYDPELDFHYRIFNADGSEVEQCGNGAR
CFARFVRLKGLINRDRIAVSTARGRISLQLEGENQVTVNMGVPQFEPGKIPFRAQKAEKTYLLRAQEHTVMCGVVSMGNP
HCVIEVPSVADAPVETLGAIMERHERFPERVNVGFMEMVNATEIRLRVFERGVGETLACGTGACAAAVIGISQGKLKERV
TVSLPGGKLTIAWKGPGQPVYMTGPAEHVFDGQIEL

Sequences:

>Translated_276_residues
MLIDFSKMHGLGNDFMVVDGVTQKVFFSNDVIKKLADRHFGIGFDQLLLVEPPYDPELDFHYRIFNADGSEVEQCGNGAR
CFARFVRLKGLINRDRIAVSTARGRISLQLEGENQVTVNMGVPQFEPGKIPFRAQKAEKTYLLRAQEHTVMCGVVSMGNP
HCVIEVPSVADAPVETLGAIMERHERFPERVNVGFMEMVNATEIRLRVFERGVGETLACGTGACAAAVIGISQGKLKERV
TVSLPGGKLTIAWKGPGQPVYMTGPAEHVFDGQIEL
>Mature_276_residues
MLIDFSKMHGLGNDFMVVDGVTQKVFFSNDVIKKLADRHFGIGFDQLLLVEPPYDPELDFHYRIFNADGSEVEQCGNGAR
CFARFVRLKGLINRDRIAVSTARGRISLQLEGENQVTVNMGVPQFEPGKIPFRAQKAEKTYLLRAQEHTVMCGVVSMGNP
HCVIEVPSVADAPVETLGAIMERHERFPERVNVGFMEMVNATEIRLRVFERGVGETLACGTGACAAAVIGISQGKLKERV
TVSLPGGKLTIAWKGPGQPVYMTGPAEHVFDGQIEL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=70.8029197080292, Blast_Score=416, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30364; Mature: 30364

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIDFSKMHGLGNDFMVVDGVTQKVFFSNDVIKKLADRHFGIGFDQLLLVEPPYDPELDF
CEEECHHHCCCCCCEEEECCCCEEEEECHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCE
HYRIFNADGSEVEQCGNGARCFARFVRLKGLINRDRIAVSTARGRISLQLEGENQVTVNM
EEEEECCCCHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCCEEEEEE
GVPQFEPGKIPFRAQKAEKTYLLRAQEHTVMCGVVSMGNPHCVIEVPSVADAPVETLGAI
CCCCCCCCCCCEEECCCCCEEEEEECCCEEEEEEEECCCCEEEEECCCCCCCCHHHHHHH
MERHERFPERVNVGFMEMVNATEIRLRVFERGVGETLACGTGACAAAVIGISQGKLKERV
HHHHHCCHHHCCCCHHHHCCHHHHEEEEECCCCCCEECCCCCHHHHHEEECCCCCCCCEE
TVSLPGGKLTIAWKGPGQPVYMTGPAEHVFDGQIEL
EEEECCCEEEEEEECCCCEEEEECCCCCCCCCEECC
>Mature Secondary Structure
MLIDFSKMHGLGNDFMVVDGVTQKVFFSNDVIKKLADRHFGIGFDQLLLVEPPYDPELDF
CEEECHHHCCCCCCEEEECCCCEEEEECHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCE
HYRIFNADGSEVEQCGNGARCFARFVRLKGLINRDRIAVSTARGRISLQLEGENQVTVNM
EEEEECCCCHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCCEEEEEE
GVPQFEPGKIPFRAQKAEKTYLLRAQEHTVMCGVVSMGNPHCVIEVPSVADAPVETLGAI
CCCCCCCCCCCEEECCCCCEEEEEECCCEEEEEEEECCCCEEEEECCCCCCCCHHHHHHH
MERHERFPERVNVGFMEMVNATEIRLRVFERGVGETLACGTGACAAAVIGISQGKLKERV
HHHHHCCHHHCCCCHHHHCCHHHHEEEEECCCCCCEECCCCCHHHHHEEECCCCCCCCEE
TVSLPGGKLTIAWKGPGQPVYMTGPAEHVFDGQIEL
EEEECCCEEEEEEECCCCEEEEECCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA