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The map label for this gene is ygfZ [C]

Identifier: 145300371

GI number: 145300371

Start: 3787298

End: 3788209

Strand: Direct

Name: ygfZ [C]

Synonym: ASA_3496

Alternate gene names: 145300371

Gene position: 3787298-3788209 (Clockwise)

Preceding gene: 145300368

Following gene: 145300373

Centisome position: 80.54

GC content: 60.86

Gene sequence:

>912_bases
GTGAGCCTGCAAACGCCTGTTTTTCCTGCCGAGCCAACCCTTTTCTCCCTGACCGATCTCGCCATCACCAGCCTGACCGG
TGCAGATCGCGTCAAGTATCTGCAAGGCCAGGTCACCTGCGATGTCAATGCACTGCAACCGGGCCAAAGCACGCTGGGCG
GCCACTGCGATGCCAAAGGCAAGCTGTGGAGCGATTTTCGTCTGCTCTACCTGAGCGATCGCCTGCTGATGCTGACCAAG
CCCTCTGTGCTGGCGCGTCAGTTGCCCGAACTCAAGAAGTTTGCGGTGTTTGCCAAGGTCGAGATCGCCGAGAACCATGG
TCAGGCAGTTGGCGTGGCTGGCCAGGGCACGGATGAGTGGATGGAGGCCCGATTTGCCGTGTCACAAACCGGCCTGATCG
ACGATGGCATGGCAGTGCAGATCGAAGCGGATCGCTGGCTGCTGGTGAGCGAGCAACCACTGACCATAGACTTGCCTGCC
GGTAGCGAATCACTCTGGTGGGGACTGGATATCAAGGCTGGCATCCCCCATCTGGAAGCCGTGCATCAGGGGGAGTACAT
ACCCCAGATGTTGAACCTGCAGGCACTGGATGGCATCAGCTTCACCAAGGGCTGCTACATGGGGCAGGAGACTGTCGCCC
GCGCCAAGTATCGCGGGGCCAACAATCGCGCCCTGTTCGTGCTGGCCGGGACTGCCAGCGAGCCCGTCGCCTGTGGCGAT
GCCCTGGAGATCCAGCTCGGTGACAACTGGCGACGCAGCGGCATGGTGCTCAACGTCTGGCAACAGGCAGATCAGGTGTG
GATCACGGCCGTGCTGCCCAAGGATACAGAGGCCGATGCCCATTTTCGTTTCAAGCAGGAGGAGGCCTCCAGCCTTGCTC
TGCAAGCCCTGCCCTACTCGCTGACCGAGTAA

Upstream 100 bases:

>100_bases
CAAAGACCCGAGGATGGTAACAGCTGGATTTTCCGGCTGACAAACATTCCTTACGTCTTTACCATGCAGGTCACATACTT
TCTGTCTCTGGAATACCATC

Downstream 100 bases:

>100_bases
GGTCGGCTAACGAGGCACTCGCGCCAGCAGTGCCTCCTTGAGCCAGCGCCCCGCCTGCCCCAGCGGGCGGGCGCGATACC
ATTGCAGCTCCAGAGCCAGC

Product: aminomethyltransferase related to GcvT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSLQTPVFPAEPTLFSLTDLAITSLTGADRVKYLQGQVTCDVNALQPGQSTLGGHCDAKGKLWSDFRLLYLSDRLLMLTK
PSVLARQLPELKKFAVFAKVEIAENHGQAVGVAGQGTDEWMEARFAVSQTGLIDDGMAVQIEADRWLLVSEQPLTIDLPA
GSESLWWGLDIKAGIPHLEAVHQGEYIPQMLNLQALDGISFTKGCYMGQETVARAKYRGANNRALFVLAGTASEPVACGD
ALEIQLGDNWRRSGMVLNVWQQADQVWITAVLPKDTEADAHFRFKQEEASSLALQALPYSLTE

Sequences:

>Translated_303_residues
MSLQTPVFPAEPTLFSLTDLAITSLTGADRVKYLQGQVTCDVNALQPGQSTLGGHCDAKGKLWSDFRLLYLSDRLLMLTK
PSVLARQLPELKKFAVFAKVEIAENHGQAVGVAGQGTDEWMEARFAVSQTGLIDDGMAVQIEADRWLLVSEQPLTIDLPA
GSESLWWGLDIKAGIPHLEAVHQGEYIPQMLNLQALDGISFTKGCYMGQETVARAKYRGANNRALFVLAGTASEPVACGD
ALEIQLGDNWRRSGMVLNVWQQADQVWITAVLPKDTEADAHFRFKQEEASSLALQALPYSLTE
>Mature_302_residues
SLQTPVFPAEPTLFSLTDLAITSLTGADRVKYLQGQVTCDVNALQPGQSTLGGHCDAKGKLWSDFRLLYLSDRLLMLTKP
SVLARQLPELKKFAVFAKVEIAENHGQAVGVAGQGTDEWMEARFAVSQTGLIDDGMAVQIEADRWLLVSEQPLTIDLPAG
SESLWWGLDIKAGIPHLEAVHQGEYIPQMLNLQALDGISFTKGCYMGQETVARAKYRGANNRALFVLAGTASEPVACGDA
LEIQLGDNWRRSGMVLNVWQQADQVWITAVLPKDTEADAHFRFKQEEASSLALQALPYSLTE

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=330, Percent_Identity=42.1212121212121, Blast_Score=221, Evalue=5e-59,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 33161; Mature: 33030

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQTPVFPAEPTLFSLTDLAITSLTGADRVKYLQGQVTCDVNALQPGQSTLGGHCDAKG
CCCCCCCCCCCCCEEEHHHHHHHHCCCCCCEEEECCEEEEEEECCCCCCHHCCCCCCCCC
KLWSDFRLLYLSDRLLMLTKPSVLARQLPELKKFAVFAKVEIAENHGQAVGVAGQGTDEW
CCCCCEEEEEEECEEEEEECCHHHHHHHHHHHHEEEEEEEEEECCCCCEEEECCCCHHHH
MEARFAVSQTGLIDDGMAVQIEADRWLLVSEQPLTIDLPAGSESLWWGLDIKAGIPHLEA
HHHHHHHHCCCCCCCCEEEEEECCEEEEEECCCEEEECCCCCCCEEEEEEECCCCCHHHH
VHQGEYIPQMLNLQALDGISFTKGCYMGQETVARAKYRGANNRALFVLAGTASEPVACGD
HHCCCCCHHHHCEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCC
ALEIQLGDNWRRSGMVLNVWQQADQVWITAVLPKDTEADAHFRFKQEEASSLALQALPYS
EEEEEECCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCEEEECHHHHHHEEEECCCCC
LTE
CCC
>Mature Secondary Structure 
SLQTPVFPAEPTLFSLTDLAITSLTGADRVKYLQGQVTCDVNALQPGQSTLGGHCDAKG
CCCCCCCCCCCCEEEHHHHHHHHCCCCCCEEEECCEEEEEEECCCCCCHHCCCCCCCCC
KLWSDFRLLYLSDRLLMLTKPSVLARQLPELKKFAVFAKVEIAENHGQAVGVAGQGTDEW
CCCCCEEEEEEECEEEEEECCHHHHHHHHHHHHEEEEEEEEEECCCCCEEEECCCCHHHH
MEARFAVSQTGLIDDGMAVQIEADRWLLVSEQPLTIDLPAGSESLWWGLDIKAGIPHLEA
HHHHHHHHCCCCCCCCEEEEEECCEEEEEECCCEEEECCCCCCCEEEEEEECCCCCHHHH
VHQGEYIPQMLNLQALDGISFTKGCYMGQETVARAKYRGANNRALFVLAGTASEPVACGD
HHCCCCCHHHHCEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCC
ALEIQLGDNWRRSGMVLNVWQQADQVWITAVLPKDTEADAHFRFKQEEASSLALQALPYS
EEEEEECCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCEEEECHHHHHHEEEECCCCC
LTE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA