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The map label for this gene is nadB [H]

Identifier: 145300368

GI number: 145300368

Start: 3784716

End: 3786323

Strand: Direct

Name: nadB [H]

Synonym: ASA_3493

Alternate gene names: 145300368

Gene position: 3784716-3786323 (Clockwise)

Preceding gene: 145300357

Following gene: 145300371

Centisome position: 80.48

GC content: 62.25

Gene sequence:

>1608_bases
ATGAAAGCAAGTGTTGAATACCTTTGCGATGTACTGATCATTGGCAGCGGTGCCGCTGGTCTGTCCCTCGCGTTGCGGTT
GGCAGACCAGGCCAAAGTCCTGGTTCTGAGCAAGGGGCCCATCAGTGAGGGGGCCACTCTCTATGCGCAAGGAGGCATCG
CTGCCGTTTTCGACGAAACCGACAGCATCGAATCCCACGTGAGCGACACGCTCAACGCCGGGGCAGGGTTGTGTGACCCT
GCCGTGGTGGAGTTCACCGCCCGCAACGCCCGCGAATCCATCCAGTGGCTGATCAAGCAGGGGGTTCCCTTCGATCAGGA
GGAGGACGCCGAGGGCGAGTCCCACTATCACCTGACCCGTGAAGGGGGCCACAGCCATCGCCGCATCTTCCATGCGGCCG
ATGCCACCGGCAAGGCGGTGCAGCTCACCCTGATCGATCAGGTGCGCTCCCACCCCAATATCCAGCTGATGGAACGCTTC
AACGCGGTCGACCTCATCACCACCCGCAAGCTGAACCTGCCGGGCAACAAGGTGCTGGGCGCCTACGTCTGGAACCGTAA
CGCCGAGCAGGTCGAGGTGATCCGCGCCCGCTTCGTGGCGCTGGCCACCGGCGGTGCCTCCAAGGTGTATCAGTACACCT
CCAACCCGGACGTGAGCTCGGGTGACGGCATCGCCATGGCGTGGCGCGCCGGCTGCCGGGTAGCGAACCTGGAGTTCAAC
CAGTTCCACCCCACCAGCCTGTTCCACCCCGATGACCCCAACTTCCTGCTGACCGAAGCCCTGCGCGGGGAAGGTGCCTA
TCTGCGCCGTCCCGACGGCAGCCGCTTCATGCTGGAGTTCGATGAACGGGCCGAGCTGGCCCCACGGGATATAGTCGCCC
GCGCCATCGACCACGAGATGAAACGCCTCGGTGCCGACTGCATGTATCTGGACATCAGCCACAAGCCGGCGGATTTCATC
ATCAAGCACTTCCCGACCATTCACGAGCGCTGCATGGCTGTCGGCATCGACATCACCAGGGAGCCGATGCCCATAGTCCC
GGCGGCCCACTACACCTGTGGTGGCGTGATGATCGACAACAATGGCCAGACCGACATTCCCGGTCTCTATGCCATTGGCG
AAGTGACCTACACGGGTCTGCACGGTGCCAACCGCATGGCCTCCAACTCCCTGCTCGAGTGCGTGGTCTATGCCCATCAG
GCGGGAGCCGACATCATGGCCAAGTTGCCCATGAGTGTGGAACCGCCCCATCTGCCCGCCTGGGATGAGAGCAAGGTGGA
CGACTCGGACGAACAGGTCGTTATCCAGCACAACTGGCATGAGCTGCGGCTGATGATGTGGGACTATGTGGGGATAGTGC
GTACCAACAAACGCCTGGCCCGCGCCAAGCGCCGTATCGACCTGCTCAAGCAGGAAGTGCAGGAGTACTACGCCAACTTC
CGCGTTTCCAACAACCTGCTGGAGCTGCGTAACCTGCTGCAGGTGGCCGAGCTCATCGTCAACTGCGCACTGCAGCGCAA
GGAGTCGCGCGGCCTGCACTACAACCTGGATTATCCGGACATGCAACCAGACTCCAAACCCACTGTGCTGACCCCGGAGC
GGGAGTAA

Upstream 100 bases:

>100_bases
GGAAAGTTCGGTTGCATTGAAAAAAATGTGACGCAGGCTGCATCACCGCCCTCGCTTGGGCTACCATGGTGCCAGTTCTT
AATCCAGGCCAATGATACTT

Downstream 100 bases:

>100_bases
TCCTGCACCAGTGATGAGCCAAGGGAGATACGCAAGCTGCACCCCACATCCCGGACTACCCGGACATACAGCCAGACTCC
ACACCCACGGTGCTGACGCC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MKASVEYLCDVLIIGSGAAGLSLALRLADQAKVLVLSKGPISEGATLYAQGGIAAVFDETDSIESHVSDTLNAGAGLCDP
AVVEFTARNARESIQWLIKQGVPFDQEEDAEGESHYHLTREGGHSHRRIFHAADATGKAVQLTLIDQVRSHPNIQLMERF
NAVDLITTRKLNLPGNKVLGAYVWNRNAEQVEVIRARFVALATGGASKVYQYTSNPDVSSGDGIAMAWRAGCRVANLEFN
QFHPTSLFHPDDPNFLLTEALRGEGAYLRRPDGSRFMLEFDERAELAPRDIVARAIDHEMKRLGADCMYLDISHKPADFI
IKHFPTIHERCMAVGIDITREPMPIVPAAHYTCGGVMIDNNGQTDIPGLYAIGEVTYTGLHGANRMASNSLLECVVYAHQ
AGADIMAKLPMSVEPPHLPAWDESKVDDSDEQVVIQHNWHELRLMMWDYVGIVRTNKRLARAKRRIDLLKQEVQEYYANF
RVSNNLLELRNLLQVAELIVNCALQRKESRGLHYNLDYPDMQPDSKPTVLTPERE

Sequences:

>Translated_535_residues
MKASVEYLCDVLIIGSGAAGLSLALRLADQAKVLVLSKGPISEGATLYAQGGIAAVFDETDSIESHVSDTLNAGAGLCDP
AVVEFTARNARESIQWLIKQGVPFDQEEDAEGESHYHLTREGGHSHRRIFHAADATGKAVQLTLIDQVRSHPNIQLMERF
NAVDLITTRKLNLPGNKVLGAYVWNRNAEQVEVIRARFVALATGGASKVYQYTSNPDVSSGDGIAMAWRAGCRVANLEFN
QFHPTSLFHPDDPNFLLTEALRGEGAYLRRPDGSRFMLEFDERAELAPRDIVARAIDHEMKRLGADCMYLDISHKPADFI
IKHFPTIHERCMAVGIDITREPMPIVPAAHYTCGGVMIDNNGQTDIPGLYAIGEVTYTGLHGANRMASNSLLECVVYAHQ
AGADIMAKLPMSVEPPHLPAWDESKVDDSDEQVVIQHNWHELRLMMWDYVGIVRTNKRLARAKRRIDLLKQEVQEYYANF
RVSNNLLELRNLLQVAELIVNCALQRKESRGLHYNLDYPDMQPDSKPTVLTPERE
>Mature_535_residues
MKASVEYLCDVLIIGSGAAGLSLALRLADQAKVLVLSKGPISEGATLYAQGGIAAVFDETDSIESHVSDTLNAGAGLCDP
AVVEFTARNARESIQWLIKQGVPFDQEEDAEGESHYHLTREGGHSHRRIFHAADATGKAVQLTLIDQVRSHPNIQLMERF
NAVDLITTRKLNLPGNKVLGAYVWNRNAEQVEVIRARFVALATGGASKVYQYTSNPDVSSGDGIAMAWRAGCRVANLEFN
QFHPTSLFHPDDPNFLLTEALRGEGAYLRRPDGSRFMLEFDERAELAPRDIVARAIDHEMKRLGADCMYLDISHKPADFI
IKHFPTIHERCMAVGIDITREPMPIVPAAHYTCGGVMIDNNGQTDIPGLYAIGEVTYTGLHGANRMASNSLLECVVYAHQ
AGADIMAKLPMSVEPPHLPAWDESKVDDSDEQVVIQHNWHELRLMMWDYVGIVRTNKRLARAKRRIDLLKQEVQEYYANF
RVSNNLLELRNLLQVAELIVNCALQRKESRGLHYNLDYPDMQPDSKPTVLTPERE

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=511, Percent_Identity=31.3111545988258, Blast_Score=195, Evalue=8e-50,
Organism=Escherichia coli, GI1788928, Length=533, Percent_Identity=72.2326454033771, Blast_Score=818, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=523, Percent_Identity=32.5047801147228, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1786942, Length=560, Percent_Identity=30.5357142857143, Blast_Score=194, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17505833, Length=534, Percent_Identity=31.4606741573034, Blast_Score=209, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17550100, Length=536, Percent_Identity=31.1567164179104, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI71986328, Length=448, Percent_Identity=29.0178571428571, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322701, Length=557, Percent_Identity=32.4955116696589, Blast_Score=217, Evalue=5e-57,
Organism=Saccharomyces cerevisiae, GI6322416, Length=531, Percent_Identity=31.4500941619586, Blast_Score=207, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6322511, Length=482, Percent_Identity=26.1410788381743, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6320788, Length=497, Percent_Identity=25.7545271629779, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17137288, Length=537, Percent_Identity=32.2160148975791, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24655642, Length=537, Percent_Identity=32.2160148975791, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24655647, Length=537, Percent_Identity=32.2160148975791, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24663005, Length=556, Percent_Identity=28.2374100719424, Blast_Score=186, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 59573; Mature: 59573

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASVEYLCDVLIIGSGAAGLSLALRLADQAKVLVLSKGPISEGATLYAQGGIAAVFDET
CCCCHHHHEEEEEECCCCCCHHHHEEECCCCEEEEEECCCCCCCCEEEECCCEEEEECCC
DSIESHVSDTLNAGAGLCDPAVVEFTARNARESIQWLIKQGVPFDQEEDAEGESHYHLTR
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEC
EGGHSHRRIFHAADATGKAVQLTLIDQVRSHPNIQLMERFNAVDLITTRKLNLPGNKVLG
CCCCCHHEEEEECCCCCCEEEEEEEHHHHCCCCHHHHHHCCCEEEEEEEEECCCCCCEEE
AYVWNRNAEQVEVIRARFVALATGGASKVYQYTSNPDVSSGDGIAMAWRAGCRVANLEFN
EEEECCCCHHHHHHHHHHHEEECCCCHHEEEECCCCCCCCCCCEEEEECCCCEEEEEECC
QFHPTSLFHPDDPNFLLTEALRGEGAYLRRPDGSRFMLEFDERAELAPRDIVARAIDHEM
CCCCCCCCCCCCCCEEEHHHHCCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
KRLGADCMYLDISHKPADFIIKHFPTIHERCMAVGIDITREPMPIVPAAHYTCGGVMIDN
HHCCCCEEEEEECCCCHHHHHHHCCHHHHHHHHCCCEECCCCCCCCCCCCEEECCEEECC
NGQTDIPGLYAIGEVTYTGLHGANRMASNSLLECVVYAHQAGADIMAKLPMSVEPPHLPA
CCCCCCCCEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCC
WDESKVDDSDEQVVIQHNWHELRLMMWDYVGIVRTNKRLARAKRRIDLLKQEVQEYYANF
CCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHCC
RVSNNLLELRNLLQVAELIVNCALQRKESRGLHYNLDYPDMQPDSKPTVLTPERE
EECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEECCCCC
>Mature Secondary Structure
MKASVEYLCDVLIIGSGAAGLSLALRLADQAKVLVLSKGPISEGATLYAQGGIAAVFDET
CCCCHHHHEEEEEECCCCCCHHHHEEECCCCEEEEEECCCCCCCCEEEECCCEEEEECCC
DSIESHVSDTLNAGAGLCDPAVVEFTARNARESIQWLIKQGVPFDQEEDAEGESHYHLTR
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEC
EGGHSHRRIFHAADATGKAVQLTLIDQVRSHPNIQLMERFNAVDLITTRKLNLPGNKVLG
CCCCCHHEEEEECCCCCCEEEEEEEHHHHCCCCHHHHHHCCCEEEEEEEEECCCCCCEEE
AYVWNRNAEQVEVIRARFVALATGGASKVYQYTSNPDVSSGDGIAMAWRAGCRVANLEFN
EEEECCCCHHHHHHHHHHHEEECCCCHHEEEECCCCCCCCCCCEEEEECCCCEEEEEECC
QFHPTSLFHPDDPNFLLTEALRGEGAYLRRPDGSRFMLEFDERAELAPRDIVARAIDHEM
CCCCCCCCCCCCCCEEEHHHHCCCCCEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
KRLGADCMYLDISHKPADFIIKHFPTIHERCMAVGIDITREPMPIVPAAHYTCGGVMIDN
HHCCCCEEEEEECCCCHHHHHHHCCHHHHHHHHCCCEECCCCCCCCCCCCEEECCEEECC
NGQTDIPGLYAIGEVTYTGLHGANRMASNSLLECVVYAHQAGADIMAKLPMSVEPPHLPA
CCCCCCCCEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCC
WDESKVDDSDEQVVIQHNWHELRLMMWDYVGIVRTNKRLARAKRRIDLLKQEVQEYYANF
CCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHCC
RVSNNLLELRNLLQVAELIVNCALQRKESRGLHYNLDYPDMQPDSKPTVLTPERE
EECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]