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The map label for this gene is ampD [H]

Identifier: 145300357

GI number: 145300357

Start: 3774916

End: 3775737

Strand: Direct

Name: ampD [H]

Synonym: ASA_3482

Alternate gene names: 145300357

Gene position: 3774916-3775737 (Clockwise)

Preceding gene: 145300356

Following gene: 145300368

Centisome position: 80.28

GC content: 66.42

Gene sequence:

>822_bases
ATGCAGCTGCGATTATGGTCACTCTGCCTCATACTGCTGCTGAGCGCCTGCCAGCCTGCCCCCTATCAGCTCTCTTCCCG
TTACCCCAGCGCCAGCCAGAATGAACGCATCGCTTTCCTGATCCTGCACTACACGGACGAGGACGACGCCCACTCACTGC
GCCTGCTGACCGAACCGGCCCATCAGGTGAGCGCTCACTACCTGATCCCCCGCGACACCGACCAGACACCGCTGCCCGTC
TACCAACTGGTGCCCGACAGCCAGCGCGCCTGGCATGCGGGCCGCAGTCGCTGGCACCAGTATGCGGGGCTCAACGCCAG
CTCCATCGGCATCGAAATCGTCAACCTGGGCTATCCGCCGGAAGATGCGCTGCTACCCGCCCACCAGCGGCGCTGGCAGC
CCTACACCCAGGCGCAGATCGCCGCCATCGGCGCCCTTACTCAGAAGCTGGTGCAACGCTACCGGATTCCGCCAACCCAG
GTGCTGGCCCACAGCGATGTCGCCCCCGAGCGCAAGCAGGATCCCGGCCCCCACTTCCCCTGGCGCGAGCTCGCCCTGCA
CTACGGGGTCGGCGCCTGGCCTGATGAAGCCAGGGTCACAGCCCTTCGCCAGCAGCCAGCACCCGCCTGGAATGCCCTCA
CCTGGCAACAGCAACTCGCCCGTTACGGTTATGGGGTCGCCCTCAGCAGCACCTGGGATGAACAGAGCCGCGCCGCCCTG
CGCGCCTTCCAGCTCCATTTCCGCCCAGCCCTGATCAGCGGCGAGCCCGACGAGCAGAGCCAGGCCATCCTCACCGCCCT
GCTGGAACGTTACTTCCCCTAA

Upstream 100 bases:

>100_bases
AGTCATGGGCCAGCGTATCTGCTCACTGTCAGCCCGTGTCCCGCTGGCCAGTGGGCGGCGCTGGCCGTCACGCCTTCCTG
AACCAGGCTCAAACGGAGCC

Downstream 100 bases:

>100_bases
GCGCCAGAGATGAAAAGGACGCCGCGGCGTCCCTTTTCATCTCTCATCAACCCAGGTCGGTCAGATCCCCTGACGGACTT
CCTGCATCAGCAGGCGCATG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MQLRLWSLCLILLLSACQPAPYQLSSRYPSASQNERIAFLILHYTDEDDAHSLRLLTEPAHQVSAHYLIPRDTDQTPLPV
YQLVPDSQRAWHAGRSRWHQYAGLNASSIGIEIVNLGYPPEDALLPAHQRRWQPYTQAQIAAIGALTQKLVQRYRIPPTQ
VLAHSDVAPERKQDPGPHFPWRELALHYGVGAWPDEARVTALRQQPAPAWNALTWQQQLARYGYGVALSSTWDEQSRAAL
RAFQLHFRPALISGEPDEQSQAILTALLERYFP

Sequences:

>Translated_273_residues
MQLRLWSLCLILLLSACQPAPYQLSSRYPSASQNERIAFLILHYTDEDDAHSLRLLTEPAHQVSAHYLIPRDTDQTPLPV
YQLVPDSQRAWHAGRSRWHQYAGLNASSIGIEIVNLGYPPEDALLPAHQRRWQPYTQAQIAAIGALTQKLVQRYRIPPTQ
VLAHSDVAPERKQDPGPHFPWRELALHYGVGAWPDEARVTALRQQPAPAWNALTWQQQLARYGYGVALSSTWDEQSRAAL
RAFQLHFRPALISGEPDEQSQAILTALLERYFP
>Mature_273_residues
MQLRLWSLCLILLLSACQPAPYQLSSRYPSASQNERIAFLILHYTDEDDAHSLRLLTEPAHQVSAHYLIPRDTDQTPLPV
YQLVPDSQRAWHAGRSRWHQYAGLNASSIGIEIVNLGYPPEDALLPAHQRRWQPYTQAQIAAIGALTQKLVQRYRIPPTQ
VLAHSDVAPERKQDPGPHFPWRELALHYGVGAWPDEARVTALRQQPAPAWNALTWQQQLARYGYGVALSSTWDEQSRAAL
RAFQLHFRPALISGEPDEQSQAILTALLERYFP

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=255, Percent_Identity=43.5294117647059, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1786300, Length=139, Percent_Identity=36.6906474820144, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 31016; Mature: 31016

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLRLWSLCLILLLSACQPAPYQLSSRYPSASQNERIAFLILHYTDEDDAHSLRLLTEPA
CCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCH
HQVSAHYLIPRDTDQTPLPVYQLVPDSQRAWHAGRSRWHQYAGLNASSIGIEIVNLGYPP
HHHHEEEECCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCC
EDALLPAHQRRWQPYTQAQIAAIGALTQKLVQRYRIPPTQVLAHSDVAPERKQDPGPHFP
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCC
WRELALHYGVGAWPDEARVTALRQQPAPAWNALTWQQQLARYGYGVALSSTWDEQSRAAL
HHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHH
RAFQLHFRPALISGEPDEQSQAILTALLERYFP
HHHHHHHCEEEECCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQLRLWSLCLILLLSACQPAPYQLSSRYPSASQNERIAFLILHYTDEDDAHSLRLLTEPA
CCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCH
HQVSAHYLIPRDTDQTPLPVYQLVPDSQRAWHAGRSRWHQYAGLNASSIGIEIVNLGYPP
HHHHEEEECCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCC
EDALLPAHQRRWQPYTQAQIAAIGALTQKLVQRYRIPPTQVLAHSDVAPERKQDPGPHFP
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCC
WRELALHYGVGAWPDEARVTALRQQPAPAWNALTWQQQLARYGYGVALSSTWDEQSRAAL
HHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHH
RAFQLHFRPALISGEPDEQSQAILTALLERYFP
HHHHHHHCEEEECCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]