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The map label for this gene is thiP [H]

Identifier: 145300298

GI number: 145300298

Start: 3697019

End: 3698626

Strand: Direct

Name: thiP [H]

Synonym: ASA_3416

Alternate gene names: 145300298

Gene position: 3697019-3698626 (Clockwise)

Preceding gene: 145300297

Following gene: 145300299

Centisome position: 78.62

GC content: 65.73

Gene sequence:

>1608_bases
GTGAGCACCAGCGTTCGTCCCCTCTGGTGGCTGCCGGGTAGCGCAGCCACCCTGTTGATCCTGCTGTTGTCCCTGGGGCC
GCTCGTCGCCCTGCTGTGGCAGGCCGGTTCGCTGGCCCCCCGCACCCTGCTGGCCGATCCCTATCTGCGCCATGTGCTGG
GCTTCAGCCTGGGGCAGGCGCTGCTCTCCACCCTGCTGAGCTTAGGTGTAGCCATTCCGGTGGCAAGAGCGCTGGCGCGG
CGCCGCTTCATTGGTCGACTCCTGCTGCTCAAGCTGTTTGGGCTCTCCCTGGTGCTGCCAGTCATCATCGCCATCTTCGG
CATAGTGGCGGTTCACGGCAAACAAGGCTGGCTATCGCAGCTGCTGCGCGGCCTGGGGCTGGATCCCGGCAACTACCTGT
ACGGCCTGTTTGGCATACTGCTGGCCCACGTCTTCTTCAACATGCCGCTGGCGGCGCGCCTCATGCTGCAGAGCATAGAG
AGCATCCCCGAATCGAGCTGGCGTCTCGCCAGTCAGTTGGGCATGCGCTCCTCCCACATCTTCCGGCTGCTGGAGTGGCC
GCTCATTCGCGGCATACTGCCGGGGCTTGCCAGCCTCATTTTCATGCTCTGCTTCACCAGCTTCACCACTGTGCTGGCGC
TGGGGGGCGGACCCAGGTCCACCACGCTGGAGGTGGCCGTCTATCAGGCGCTGCGCTTTGATTTCGACCTCGCTACCGCC
GGCGGCCTGGCGCTGGTGCAGTTGATCCTGACCGCCACCCTGCTCCTGCTGCAACACAAGCTGCAGACCACACCGGCCAG
CCGGATCAGCAGCCATCGCCCCTGTCTTCGACCGGATCGCCATCAACCGGGGACCGTGCTGGTGGATGCCGTGGCGCTCT
GCACCGGGCTCGCCATCTTCCTGCCGCCTCTGCTGGCAATCCTGGTGGCCGGTTTCAATCCCGGCCTGCTGACGGCGCTG
GCCTCGGCGCAGCTGTGGCAGGCCAGCGGCCAGTCGCTGCTGATCGCGCTGGCGGCGGGCACCCTGGCCACCCTGCTCGG
CAGCGGCCTGCTGCTCACCAGCCGTCACCTCAAGGTACGCACCCGTCAGCGGCGTGCCGCAGCCCTGTGGGAAGCCAGTG
GATCCATGATCCTGATGATACCGGCGGTGGTGCTCTCCACTGGCCTCTTCATCATGTTCATGCCCTTTACCGATGTCTTT
GCCCTCGGCCCCTGGCTAGTGGTACTGGTCAACGCCCTGATGGCGCTGCCCTATGTGCTGCGCACCCTCTCAGCCCCCAT
GCAGCTGGTGGTGCGCCAGTACGATAGACTGGCCGACAGCCTGGGGGTGCGCGGTCTGCATCGCCTGCGGCTGGTGGAGT
GGCCCCTGCTGCGCCGCCCGCTGGCACTGGCGATGGCGCTCTCGATGATACTGTCGCTCGGGGATCTCGGCGCCATCGCC
ATGTTTGGCAGCCAGGAGCTCACCACCCTGCCCTGGCTGCTCTATCAGCAGCTCGGCAGTTACCGGCTGACCGAGGCGGC
CGCCACCGCACTGCTGCTGCTCACGCTCTGTTTTTCCCTGTTCTGGCTGGTAGAACGCGGGCTTGGAGGAAAAAATGCTG
AACATTGA

Upstream 100 bases:

>100_bases
AGCAGATGATCACAGTCAGCAAGCCGCTGAGTTTCACCAGCGATGAGGTGGCCGCCAATCGCAAGAACTGGATCCGCGAG
TGGCTGCAAGCCGTCACCCA

Downstream 100 bases:

>100_bases
TGGGCTGGTCACCAGCTACCCCGATTGGCGAGTCAGCTTCAGCGCGACCCTGCCCAAGGGAGAGATCACCGCGCTGATAG
GGCCAAGCGGGGCGGGCAAG

Product: ABC-type thiamine transporter, permease protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MSTSVRPLWWLPGSAATLLILLLSLGPLVALLWQAGSLAPRTLLADPYLRHVLGFSLGQALLSTLLSLGVAIPVARALAR
RRFIGRLLLLKLFGLSLVLPVIIAIFGIVAVHGKQGWLSQLLRGLGLDPGNYLYGLFGILLAHVFFNMPLAARLMLQSIE
SIPESSWRLASQLGMRSSHIFRLLEWPLIRGILPGLASLIFMLCFTSFTTVLALGGGPRSTTLEVAVYQALRFDFDLATA
GGLALVQLILTATLLLLQHKLQTTPASRISSHRPCLRPDRHQPGTVLVDAVALCTGLAIFLPPLLAILVAGFNPGLLTAL
ASAQLWQASGQSLLIALAAGTLATLLGSGLLLTSRHLKVRTRQRRAAALWEASGSMILMIPAVVLSTGLFIMFMPFTDVF
ALGPWLVVLVNALMALPYVLRTLSAPMQLVVRQYDRLADSLGVRGLHRLRLVEWPLLRRPLALAMALSMILSLGDLGAIA
MFGSQELTTLPWLLYQQLGSYRLTEAAATALLLLTLCFSLFWLVERGLGGKNAEH

Sequences:

>Translated_535_residues
MSTSVRPLWWLPGSAATLLILLLSLGPLVALLWQAGSLAPRTLLADPYLRHVLGFSLGQALLSTLLSLGVAIPVARALAR
RRFIGRLLLLKLFGLSLVLPVIIAIFGIVAVHGKQGWLSQLLRGLGLDPGNYLYGLFGILLAHVFFNMPLAARLMLQSIE
SIPESSWRLASQLGMRSSHIFRLLEWPLIRGILPGLASLIFMLCFTSFTTVLALGGGPRSTTLEVAVYQALRFDFDLATA
GGLALVQLILTATLLLLQHKLQTTPASRISSHRPCLRPDRHQPGTVLVDAVALCTGLAIFLPPLLAILVAGFNPGLLTAL
ASAQLWQASGQSLLIALAAGTLATLLGSGLLLTSRHLKVRTRQRRAAALWEASGSMILMIPAVVLSTGLFIMFMPFTDVF
ALGPWLVVLVNALMALPYVLRTLSAPMQLVVRQYDRLADSLGVRGLHRLRLVEWPLLRRPLALAMALSMILSLGDLGAIA
MFGSQELTTLPWLLYQQLGSYRLTEAAATALLLLTLCFSLFWLVERGLGGKNAEH
>Mature_534_residues
STSVRPLWWLPGSAATLLILLLSLGPLVALLWQAGSLAPRTLLADPYLRHVLGFSLGQALLSTLLSLGVAIPVARALARR
RFIGRLLLLKLFGLSLVLPVIIAIFGIVAVHGKQGWLSQLLRGLGLDPGNYLYGLFGILLAHVFFNMPLAARLMLQSIES
IPESSWRLASQLGMRSSHIFRLLEWPLIRGILPGLASLIFMLCFTSFTTVLALGGGPRSTTLEVAVYQALRFDFDLATAG
GLALVQLILTATLLLLQHKLQTTPASRISSHRPCLRPDRHQPGTVLVDAVALCTGLAIFLPPLLAILVAGFNPGLLTALA
SAQLWQASGQSLLIALAAGTLATLLGSGLLLTSRHLKVRTRQRRAAALWEASGSMILMIPAVVLSTGLFIMFMPFTDVFA
LGPWLVVLVNALMALPYVLRTLSAPMQLVVRQYDRLADSLGVRGLHRLRLVEWPLLRRPLALAMALSMILSLGDLGAIAM
FGSQELTTLPWLLYQQLGSYRLTEAAATALLLLTLCFSLFWLVERGLGGKNAEH

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=537, Percent_Identity=46.927374301676, Blast_Score=386, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 57925; Mature: 57793

Theoretical pI: Translated: 11.50; Mature: 11.50

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSVRPLWWLPGSAATLLILLLSLGPLVALLWQAGSLAPRTLLADPYLRHVLGFSLGQA
CCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHH
LLSTLLSLGVAIPVARALARRRFIGRLLLLKLFGLSLVLPVIIAIFGIVAVHGKQGWLSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LLRGLGLDPGNYLYGLFGILLAHVFFNMPLAARLMLQSIESIPESSWRLASQLGMRSSHI
HHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
FRLLEWPLIRGILPGLASLIFMLCFTSFTTVLALGGGPRSTTLEVAVYQALRFDFDLATA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHCC
GGLALVQLILTATLLLLQHKLQTTPASRISSHRPCLRPDRHQPGTVLVDAVALCTGLAIF
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LPPLLAILVAGFNPGLLTALASAQLWQASGQSLLIALAAGTLATLLGSGLLLTSRHLKVR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCHHEEHHHHHHH
TRQRRAAALWEASGSMILMIPAVVLSTGLFIMFMPFTDVFALGPWLVVLVNALMALPYVL
HHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTLSAPMQLVVRQYDRLADSLGVRGLHRLRLVEWPLLRRPLALAMALSMILSLGDLGAIA
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
MFGSQELTTLPWLLYQQLGSYRLTEAAATALLLLTLCFSLFWLVERGLGGKNAEH
HCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
STSVRPLWWLPGSAATLLILLLSLGPLVALLWQAGSLAPRTLLADPYLRHVLGFSLGQA
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHH
LLSTLLSLGVAIPVARALARRRFIGRLLLLKLFGLSLVLPVIIAIFGIVAVHGKQGWLSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LLRGLGLDPGNYLYGLFGILLAHVFFNMPLAARLMLQSIESIPESSWRLASQLGMRSSHI
HHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
FRLLEWPLIRGILPGLASLIFMLCFTSFTTVLALGGGPRSTTLEVAVYQALRFDFDLATA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHCC
GGLALVQLILTATLLLLQHKLQTTPASRISSHRPCLRPDRHQPGTVLVDAVALCTGLAIF
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LPPLLAILVAGFNPGLLTALASAQLWQASGQSLLIALAAGTLATLLGSGLLLTSRHLKVR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCHHEEHHHHHHH
TRQRRAAALWEASGSMILMIPAVVLSTGLFIMFMPFTDVFALGPWLVVLVNALMALPYVL
HHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTLSAPMQLVVRQYDRLADSLGVRGLHRLRLVEWPLLRRPLALAMALSMILSLGDLGAIA
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
MFGSQELTTLPWLLYQQLGSYRLTEAAATALLLLTLCFSLFWLVERGLGGKNAEH
HCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]