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The map label for this gene is thiB [H]

Identifier: 145300297

GI number: 145300297

Start: 3696042

End: 3697022

Strand: Direct

Name: thiB [H]

Synonym: ASA_3415

Alternate gene names: 145300297

Gene position: 3696042-3697022 (Clockwise)

Preceding gene: 145300295

Following gene: 145300298

Centisome position: 78.6

GC content: 59.73

Gene sequence:

>981_bases
ATGAAAACCCTGCTGCTGGTTGCCACCAGCCTGCTCAGTACCCAGGCCTTCGCGGCTGACACCCTCACCGTTTATACCTA
CAGCTCCTTCACCGCCAAATGGGGACCCGGTCCCAAGATCAAGCAGGCATTCGAGAAAGAGTGCGGTTGCAACCTCAATC
TTGTGGCGCTGGAAGATGGCGTCGCCATCCTCAACCGGCTGCGGCTGGAAGGCAAACACAGCAAGGCCGATCTGGTGCTG
GGGCTTGATGATGCCCTCATCAGCGAAGCCAAGCAGAGCGGCCTGTTCGCCCCGCACACGACCAAGCTCGATGGCATCAA
GGTGCCGGGCGGCTGGCAGGATGACACCTTCGTCCCCTATGACTACGGCTACTTCGCCTTCGTCTACAACAAGGACAAAC
TCAAAGCGCCCCCCAAGAGCCTGAAAGAGCTGGTAGAACGCCCGGATCTCAAGGTGATCTATCAGGATCCCCGCACATCT
ACCCCGGGTCAGGGGCTGATGCTGTGGATGAAGTCCATTTATGGCGAGCAGGCGCCTGCCGCCTGGACCGAGCTTGCCAA
GAAGACGGTCACTGTGACCAAGGGCTGGTCCGAAGCCTATGGCATGTTCCTCGATGGGGAAGCGGACATGGTACTCTCCT
ACACCAGCTCCCCCGCCTATCACCTGATTGCCGAGAACAAGCCCCAGTATCAGGCCGCCGCCTTCGAAGAGGGGCACTAC
CGTCAGGTGGAAGTGGCCGCCAAGCTGAAAAGCGCCAAACAGGGCAAGCTGGCCGACCAGTTTCTGCAGTTCATGATAAG
CCCCGCCTTCCAGCAGGAGATCCCTGCCGGCAACTGGATGTATCCGGTGACAGATGCGCCGCTGCCCAAGGGCTTCGAGC
AGATGATCACAGTCAGCAAGCCGCTGAGTTTCACCAGCGATGAGGTGGCCGCCAATCGCAAGAACTGGATCCGCGAGTGG
CTGCAAGCCGTCACCCAGTGA

Upstream 100 bases:

>100_bases
TTGCGCTCTCATCCGCTGGCACCTCATGACTCATAGCAAGAAGTGCCATTGATGACATTTCCTCAGTTGGCACTTCACTA
CTAACAGGGAGTGCCGCTTG

Downstream 100 bases:

>100_bases
GCACCAGCGTTCGTCCCCTCTGGTGGCTGCCGGGTAGCGCAGCCACCCTGTTGATCCTGCTGTTGTCCCTGGGGCCGCTC
GTCGCCCTGCTGTGGCAGGC

Product: ABC-type thiamine transporter, periplasmic binding protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKTLLLVATSLLSTQAFAADTLTVYTYSSFTAKWGPGPKIKQAFEKECGCNLNLVALEDGVAILNRLRLEGKHSKADLVL
GLDDALISEAKQSGLFAPHTTKLDGIKVPGGWQDDTFVPYDYGYFAFVYNKDKLKAPPKSLKELVERPDLKVIYQDPRTS
TPGQGLMLWMKSIYGEQAPAAWTELAKKTVTVTKGWSEAYGMFLDGEADMVLSYTSSPAYHLIAENKPQYQAAAFEEGHY
RQVEVAAKLKSAKQGKLADQFLQFMISPAFQQEIPAGNWMYPVTDAPLPKGFEQMITVSKPLSFTSDEVAANRKNWIREW
LQAVTQ

Sequences:

>Translated_326_residues
MKTLLLVATSLLSTQAFAADTLTVYTYSSFTAKWGPGPKIKQAFEKECGCNLNLVALEDGVAILNRLRLEGKHSKADLVL
GLDDALISEAKQSGLFAPHTTKLDGIKVPGGWQDDTFVPYDYGYFAFVYNKDKLKAPPKSLKELVERPDLKVIYQDPRTS
TPGQGLMLWMKSIYGEQAPAAWTELAKKTVTVTKGWSEAYGMFLDGEADMVLSYTSSPAYHLIAENKPQYQAAAFEEGHY
RQVEVAAKLKSAKQGKLADQFLQFMISPAFQQEIPAGNWMYPVTDAPLPKGFEQMITVSKPLSFTSDEVAANRKNWIREW
LQAVTQ
>Mature_326_residues
MKTLLLVATSLLSTQAFAADTLTVYTYSSFTAKWGPGPKIKQAFEKECGCNLNLVALEDGVAILNRLRLEGKHSKADLVL
GLDDALISEAKQSGLFAPHTTKLDGIKVPGGWQDDTFVPYDYGYFAFVYNKDKLKAPPKSLKELVERPDLKVIYQDPRTS
TPGQGLMLWMKSIYGEQAPAAWTELAKKTVTVTKGWSEAYGMFLDGEADMVLSYTSSPAYHLIAENKPQYQAAAFEEGHY
RQVEVAAKLKSAKQGKLADQFLQFMISPAFQQEIPAGNWMYPVTDAPLPKGFEQMITVSKPLSFTSDEVAANRKNWIREW
LQAVTQ

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=311, Percent_Identity=64.3086816720257, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36253; Mature: 36253

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLLLVATSLLSTQAFAADTLTVYTYSSFTAKWGPGPKIKQAFEKECGCNLNLVALEDG
CCHHHHHHHHHHHHHHHHCCEEEEEEEECCEECCCCCHHHHHHHHHHHCCCEEEEEECCC
VAILNRLRLEGKHSKADLVLGLDDALISEAKQSGLFAPHTTKLDGIKVPGGWQDDTFVPY
HHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCEE
DYGYFAFVYNKDKLKAPPKSLKELVERPDLKVIYQDPRTSTPGQGLMLWMKSIYGEQAPA
CCCEEEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCH
AWTELAKKTVTVTKGWSEAYGMFLDGEADMVLSYTSSPAYHLIAENKPQYQAAAFEEGHY
HHHHHHHHEEEEECCHHHHCCEEECCCCCEEEEECCCCCEEEEECCCCCCHHHHCCCCCC
RQVEVAAKLKSAKQGKLADQFLQFMISPAFQQEIPAGNWMYPVTDAPLPKGFEQMITVSK
EEHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHCC
PLSFTSDEVAANRKNWIREWLQAVTQ
CCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTLLLVATSLLSTQAFAADTLTVYTYSSFTAKWGPGPKIKQAFEKECGCNLNLVALEDG
CCHHHHHHHHHHHHHHHHCCEEEEEEEECCEECCCCCHHHHHHHHHHHCCCEEEEEECCC
VAILNRLRLEGKHSKADLVLGLDDALISEAKQSGLFAPHTTKLDGIKVPGGWQDDTFVPY
HHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCEE
DYGYFAFVYNKDKLKAPPKSLKELVERPDLKVIYQDPRTSTPGQGLMLWMKSIYGEQAPA
CCCEEEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCH
AWTELAKKTVTVTKGWSEAYGMFLDGEADMVLSYTSSPAYHLIAENKPQYQAAAFEEGHY
HHHHHHHHEEEEECCHHHHCCEEECCCCCEEEEECCCCCEEEEECCCCCCHHHHCCCCCC
RQVEVAAKLKSAKQGKLADQFLQFMISPAFQQEIPAGNWMYPVTDAPLPKGFEQMITVSK
EEHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHCC
PLSFTSDEVAANRKNWIREWLQAVTQ
CCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]