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The map label for this gene is leuD [H]

Identifier: 145300294

GI number: 145300294

Start: 3693656

End: 3694255

Strand: Direct

Name: leuD [H]

Synonym: ASA_3412

Alternate gene names: 145300294

Gene position: 3693656-3694255 (Clockwise)

Preceding gene: 145300293

Following gene: 145300295

Centisome position: 78.55

GC content: 57.67

Gene sequence:

>600_bases
ATGACAGGATTCAAACAACATAAAGGGATCGCCGTTCCCCTCGACAGCGCCAACGTCGACACCGACGCCATCATTCCCAA
GCAGTTTTTGCAGAAGGTCAACCGCATCGGTTTTGGCAAGCACCTGTTCCACGACTGGCGCTTTCTCGATGATGCGGGTC
AGCAGCCCAATCCCGAGTTCGTGCTCAACCAGCCGCGCTATGCCGGTGCCAGCATCTTGCTGGCCCGGGAAAACTTTGGC
TGCGGTTCGAGCCGTGAGCACGCCCCCTGGGCACTGGCGGATTACGGCTTCAAAACCCTGATCGCCTCAAGCTTTGCCGA
TATCTTCTATGGCAACGCCATCAACAACGGCCTGGTACCGGTGCGGTTGAAAGAAGAGGAGGTGGATCTCCTGTTCCAGC
TGGTTGCCACACAGCCGGGCATCGAAATCGAGGTGGATCTGGAAGCCAATCAGGTGCGTGCCGGCGAGCTCAGTTTTGGT
TTCGAGATCGACGAGTTTCGTCGCTATTGCCTGCTCAACGGACTGGATGCCATCGGTCTGACCCTGCAGCACGAGGCCAC
CATCAGCGCCTTCGAAGCAAAGCAGCCAAGCTGGATCTGA

Upstream 100 bases:

>100_bases
CCGTGCCGGCCGCACCCATCTGGTGAGCCCGGCCATGGCCGCCGCCGCCGCCGTCACCGGTCGCTTTGCCGACATTCGCG
CACTGTAAGAGGAAATAGCG

Downstream 100 bases:

>100_bases
ACTGATCGCATCCCCTTGTAACAAGACGCGGCCATCAAAAATAAAGCGAGTAGAATCAACAGGGAGCCTTGGCTCCCTGT
TTTATTATCCCGCCGCCATG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MTGFKQHKGIAVPLDSANVDTDAIIPKQFLQKVNRIGFGKHLFHDWRFLDDAGQQPNPEFVLNQPRYAGASILLARENFG
CGSSREHAPWALADYGFKTLIASSFADIFYGNAINNGLVPVRLKEEEVDLLFQLVATQPGIEIEVDLEANQVRAGELSFG
FEIDEFRRYCLLNGLDAIGLTLQHEATISAFEAKQPSWI

Sequences:

>Translated_199_residues
MTGFKQHKGIAVPLDSANVDTDAIIPKQFLQKVNRIGFGKHLFHDWRFLDDAGQQPNPEFVLNQPRYAGASILLARENFG
CGSSREHAPWALADYGFKTLIASSFADIFYGNAINNGLVPVRLKEEEVDLLFQLVATQPGIEIEVDLEANQVRAGELSFG
FEIDEFRRYCLLNGLDAIGLTLQHEATISAFEAKQPSWI
>Mature_198_residues
TGFKQHKGIAVPLDSANVDTDAIIPKQFLQKVNRIGFGKHLFHDWRFLDDAGQQPNPEFVLNQPRYAGASILLARENFGC
GSSREHAPWALADYGFKTLIASSFADIFYGNAINNGLVPVRLKEEEVDLLFQLVATQPGIEIEVDLEANQVRAGELSFGF
EIDEFRRYCLLNGLDAIGLTLQHEATISAFEAKQPSWI

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=196, Percent_Identity=70.9183673469388, Blast_Score=303, Evalue=4e-84,
Organism=Saccharomyces cerevisiae, GI6321429, Length=198, Percent_Identity=49.4949494949495, Blast_Score=185, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22181; Mature: 22050

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGFKQHKGIAVPLDSANVDTDAIIPKQFLQKVNRIGFGKHLFHDWRFLDDAGQQPNPEF
CCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCE
VLNQPRYAGASILLARENFGCGSSREHAPWALADYGFKTLIASSFADIFYGNAINNGLVP
EECCCCCCCEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
VRLKEEEVDLLFQLVATQPGIEIEVDLEANQVRAGELSFGFEIDEFRRYCLLNGLDAIGL
EEECHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCEECCCCHHHHHHHHHHCCCCHHCE
TLQHEATISAFEAKQPSWI
EEECCCHHHHHHCCCCCCC
>Mature Secondary Structure 
TGFKQHKGIAVPLDSANVDTDAIIPKQFLQKVNRIGFGKHLFHDWRFLDDAGQQPNPEF
CCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCE
VLNQPRYAGASILLARENFGCGSSREHAPWALADYGFKTLIASSFADIFYGNAINNGLVP
EECCCCCCCEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
VRLKEEEVDLLFQLVATQPGIEIEVDLEANQVRAGELSFGFEIDEFRRYCLLNGLDAIGL
EEECHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCEECCCCHHHHHHHHHHCCCCHHCE
TLQHEATISAFEAKQPSWI
EEECCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA