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The map label for this gene is leuC [H]

Identifier: 145300293

GI number: 145300293

Start: 3692246

End: 3693643

Strand: Direct

Name: leuC [H]

Synonym: ASA_3411

Alternate gene names: 145300293

Gene position: 3692246-3693643 (Clockwise)

Preceding gene: 145300292

Following gene: 145300294

Centisome position: 78.52

GC content: 63.88

Gene sequence:

>1398_bases
ATGGCCAAAACCCTTTATCAGAAAGTATTCGACGCCCACGTGGTACGCGAAGTGGACGGCGAAACGCCGCTCATCTACAT
CGACCGCCACCTGGTACACGAAGTGACCAGCCCGCAGGCGTTCGATGGCCTGCGCGCCATGAACCGCCCGCTGCGTCGCC
CGGATCTGACCTGGGCCACCATGGATCACAACGTCTCCACCACCACCAAGGACATCGCCGCCTCCGGCGAGATGGCCCGC
ATCCAGATGGAGACGCTCGCCGACAACTGCAAGGAGTTCGGTGTTCGCCTCTACGATCTCAACCACAAGTATCAGGGGAT
CGTCCATGTGATGGGCCCCGAGCTCGGCATCACATTGCCCGGCACCACCATCGTCTGCGGCGACTCCCACACCGCCACCC
ACGGTGCCTTCGGTTCCCTGGCCTTCGGTATCGGCACCTCCGAAGTGGAACATGTGATGGCCACCCAGACCCTGAAACAG
GGCCGCGCCAAGACAATGCGCATTTCGGTCAACGGCAAGCTGGCGGAGGGCATCAGCGCCAAGGACGTGGTGCTGGCCAT
CATCGGCCAGGTTGGCCATGCCGGTGGTACCGGCTACGTGGTGGAATTCGCTGGCGAGGCCATTGCGGGCCTCTCCATGG
AAGGGCGGATGACGGTGTGCAACATGGCCATCGAGCTGGGTGCCAAGGCGGGCATGATAGCCCCCGACCAGACCACCATC
GACTACATCCGTGGCAAGGAGTTCGCCCCCAAGGGTGAATCACTGGATCAGGCCATCGCCTACTGGCTGAGCCTCAAGAG
CGACCAGGGCGCCCACTTCGATGCCGAAGTGATCCTCGACGCCGCCGATATCGCCCCGCAGGTCACCTGGGGTACCAACC
CGGGCCAGGTGATTGCCGTCAATGAACCGATCCCGGCACCGGAATCCTTCAGCGATCTGATGGAGCAGCAATCCGCCCGC
AAGGCGCTCGCCTACATGGACCTGCAACCGGGCCAGAAGCTCACCGATGTCGCCATCGACAAGGTGTTCATCGGTTCTTG
TACCAACAGCCGCATCGAGGATCTGCGTGCCGCCGCCGCCATCGCCCGTGGCCGCAAAGTCGCCGCTGGCGTACAAGCGC
TGGTGGTACCGGGCTCCGAACAGGTGAAGGCCCAGGCTGAAGCCGAGGGTCTGGACAAGATCTTCCTCGAGGCGGGTTTT
GAATGGCGTCTGCCCGGCTGTTCCATGTGTCTGGCCATGAACAACGACCGGCTGCAACCGGGGGAGCGCTGCGCCTCCAC
CAGCAACCGCAACTTCGAAGGGCGTCAGGGCCGTGCCGGCCGCACCCATCTGGTGAGCCCGGCCATGGCCGCCGCCGCCG
CCGTCACCGGTCGCTTTGCCGACATTCGCGCACTGTAA

Upstream 100 bases:

>100_bases
AGGCAGATGCTCGCCATCCGGTGCAGAGTACCGCCGACATGGGCACCCAGATCGCCGCACGGATCTGAGCCACCCAGCAC
ATAACAAGGAATACAGAGCA

Downstream 100 bases:

>100_bases
GAGGAAATAGCGATGACAGGATTCAAACAACATAAAGGGATCGCCGTTCCCCTCGACAGCGCCAACGTCGACACCGACGC
CATCATTCCCAAGCAGTTTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MAKTLYQKVFDAHVVREVDGETPLIYIDRHLVHEVTSPQAFDGLRAMNRPLRRPDLTWATMDHNVSTTTKDIAASGEMAR
IQMETLADNCKEFGVRLYDLNHKYQGIVHVMGPELGITLPGTTIVCGDSHTATHGAFGSLAFGIGTSEVEHVMATQTLKQ
GRAKTMRISVNGKLAEGISAKDVVLAIIGQVGHAGGTGYVVEFAGEAIAGLSMEGRMTVCNMAIELGAKAGMIAPDQTTI
DYIRGKEFAPKGESLDQAIAYWLSLKSDQGAHFDAEVILDAADIAPQVTWGTNPGQVIAVNEPIPAPESFSDLMEQQSAR
KALAYMDLQPGQKLTDVAIDKVFIGSCTNSRIEDLRAAAAIARGRKVAAGVQALVVPGSEQVKAQAEAEGLDKIFLEAGF
EWRLPGCSMCLAMNNDRLQPGERCASTSNRNFEGRQGRAGRTHLVSPAMAAAAAVTGRFADIRAL

Sequences:

>Translated_465_residues
MAKTLYQKVFDAHVVREVDGETPLIYIDRHLVHEVTSPQAFDGLRAMNRPLRRPDLTWATMDHNVSTTTKDIAASGEMAR
IQMETLADNCKEFGVRLYDLNHKYQGIVHVMGPELGITLPGTTIVCGDSHTATHGAFGSLAFGIGTSEVEHVMATQTLKQ
GRAKTMRISVNGKLAEGISAKDVVLAIIGQVGHAGGTGYVVEFAGEAIAGLSMEGRMTVCNMAIELGAKAGMIAPDQTTI
DYIRGKEFAPKGESLDQAIAYWLSLKSDQGAHFDAEVILDAADIAPQVTWGTNPGQVIAVNEPIPAPESFSDLMEQQSAR
KALAYMDLQPGQKLTDVAIDKVFIGSCTNSRIEDLRAAAAIARGRKVAAGVQALVVPGSEQVKAQAEAEGLDKIFLEAGF
EWRLPGCSMCLAMNNDRLQPGERCASTSNRNFEGRQGRAGRTHLVSPAMAAAAAVTGRFADIRAL
>Mature_464_residues
AKTLYQKVFDAHVVREVDGETPLIYIDRHLVHEVTSPQAFDGLRAMNRPLRRPDLTWATMDHNVSTTTKDIAASGEMARI
QMETLADNCKEFGVRLYDLNHKYQGIVHVMGPELGITLPGTTIVCGDSHTATHGAFGSLAFGIGTSEVEHVMATQTLKQG
RAKTMRISVNGKLAEGISAKDVVLAIIGQVGHAGGTGYVVEFAGEAIAGLSMEGRMTVCNMAIELGAKAGMIAPDQTTID
YIRGKEFAPKGESLDQAIAYWLSLKSDQGAHFDAEVILDAADIAPQVTWGTNPGQVIAVNEPIPAPESFSDLMEQQSARK
ALAYMDLQPGQKLTDVAIDKVFIGSCTNSRIEDLRAAAAIARGRKVAAGVQALVVPGSEQVKAQAEAEGLDKIFLEAGFE
WRLPGCSMCLAMNNDRLQPGERCASTSNRNFEGRQGRAGRTHLVSPAMAAAAAVTGRFADIRAL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=399, Percent_Identity=26.8170426065163, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI8659555, Length=367, Percent_Identity=26.4305177111717, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI41352693, Length=368, Percent_Identity=25.2717391304348, Blast_Score=89, Evalue=8e-18,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=78.7096774193548, Blast_Score=755, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=357, Percent_Identity=28.2913165266106, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI87081781, Length=324, Percent_Identity=25, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI2367097, Length=111, Percent_Identity=35.1351351351351, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=399, Percent_Identity=28.5714285714286, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=399, Percent_Identity=28.5714285714286, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=317, Percent_Identity=29.3375394321767, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=393, Percent_Identity=25.6997455470738, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=59.3617021276596, Blast_Score=572, Evalue=1e-164,
Organism=Saccharomyces cerevisiae, GI6320440, Length=427, Percent_Identity=27.1662763466042, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6323335, Length=366, Percent_Identity=30.6010928961749, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6322261, Length=399, Percent_Identity=27.0676691729323, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281365315, Length=398, Percent_Identity=27.1356783919598, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI17864292, Length=398, Percent_Identity=27.1356783919598, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI161076999, Length=398, Percent_Identity=27.1356783919598, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI28571643, Length=398, Percent_Identity=26.1306532663317, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24645686, Length=371, Percent_Identity=26.6846361185984, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17137564, Length=369, Percent_Identity=26.2872628726287, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 49805; Mature: 49674

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTLYQKVFDAHVVREVDGETPLIYIDRHLVHEVTSPQAFDGLRAMNRPLRRPDLTWAT
CCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEE
MDHNVSTTTKDIAASGEMARIQMETLADNCKEFGVRLYDLNHKYQGIVHVMGPELGITLP
ECCCCCCCHHHHHCCCCEEEEEHHHHHHHHHHHCEEEEECCCCCCEEEEEECCCCEEECC
GTTIVCGDSHTATHGAFGSLAFGIGTSEVEHVMATQTLKQGRAKTMRISVNGKLAEGISA
CCEEEECCCCCCCCCCCHHHEECCCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHCCCCH
KDVVLAIIGQVGHAGGTGYVVEFAGEAIAGLSMEGRMTVCNMAIELGAKAGMIAPDQTTI
HHHHHHHHHHHCCCCCCCEEEEECCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHH
DYIRGKEFAPKGESLDQAIAYWLSLKSDQGAHFDAEVILDAADIAPQVTWGTNPGQVIAV
HHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHEEEEHHHCCCEEEECCCCCEEEEE
NEPIPAPESFSDLMEQQSARKALAYMDLQPGQKLTDVAIDKVFIGSCTNSRIEDLRAAAA
CCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHEEEECCCCHHHHHHHHHHH
IARGRKVAAGVQALVVPGSEQVKAQAEAEGLDKIFLEAGFEWRLPGCSMCLAMNNDRLQP
HHCCCHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEECCCCCCC
GERCASTSNRNFEGRQGRAGRTHLVSPAMAAAAAVTGRFADIRAL
HHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHCCCHHEECC
>Mature Secondary Structure 
AKTLYQKVFDAHVVREVDGETPLIYIDRHLVHEVTSPQAFDGLRAMNRPLRRPDLTWAT
CHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEE
MDHNVSTTTKDIAASGEMARIQMETLADNCKEFGVRLYDLNHKYQGIVHVMGPELGITLP
ECCCCCCCHHHHHCCCCEEEEEHHHHHHHHHHHCEEEEECCCCCCEEEEEECCCCEEECC
GTTIVCGDSHTATHGAFGSLAFGIGTSEVEHVMATQTLKQGRAKTMRISVNGKLAEGISA
CCEEEECCCCCCCCCCCHHHEECCCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHCCCCH
KDVVLAIIGQVGHAGGTGYVVEFAGEAIAGLSMEGRMTVCNMAIELGAKAGMIAPDQTTI
HHHHHHHHHHHCCCCCCCEEEEECCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHH
DYIRGKEFAPKGESLDQAIAYWLSLKSDQGAHFDAEVILDAADIAPQVTWGTNPGQVIAV
HHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHEEEEHHHCCCEEEECCCCCEEEEE
NEPIPAPESFSDLMEQQSARKALAYMDLQPGQKLTDVAIDKVFIGSCTNSRIEDLRAAAA
CCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHEEEECCCCHHHHHHHHHHH
IARGRKVAAGVQALVVPGSEQVKAQAEAEGLDKIFLEAGFEWRLPGCSMCLAMNNDRLQP
HHCCCHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEECCCCCCC
GERCASTSNRNFEGRQGRAGRTHLVSPAMAAAAAVTGRFADIRAL
HHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHCCCHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA