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The map label for this gene is tcpI [H]

Identifier: 145300142

GI number: 145300142

Start: 3509598

End: 3511541

Strand: Direct

Name: tcpI [H]

Synonym: ASA_3245

Alternate gene names: 145300142

Gene position: 3509598-3511541 (Clockwise)

Preceding gene: 145300141

Following gene: 145300143

Centisome position: 74.63

GC content: 64.81

Gene sequence:

>1944_bases
GTGAAAATTGCCCACTATGCCTCCCTCTCCTCCCTGGTACTGCTGCTGGTCGCTGGCGTACAGGTCGGCGCCCTGTTTCA
TGGCTGGCAACAGCTGGATCAGGCCGAGCAAGCCCAGCATCAACATGAACGGCTGCAACAACGGCTGACCGATACCCTGC
AAGGCACCTTGCGCGACTACCTGAGCAGCGGCGATCCCCTGCGCCTGGCCGAAGCCCAGACGGTGCGTCAGCTGGCGCTC
GGCCAACTGGCACAGCAGGATGAACAGCTGACAGCCCCGCTGCGCGCCCTGCTCGAGAAGATGGGAGAGCGTATCGATGG
CGATTATCTGGCGGCGGGCAAGCTCTCTGGCAACAGCCAGTGGCTGTTGCAAAATGCCGAGAACGAACTGACCACCCAGG
CCAGGGCGCTGCTGCGCTACGGCCAGCAGGGAGCCATGGTTCCGGGTACGGATCAGGCCAGTGCCGAGGCTTACCGCAAA
GGGGCGGCCGATCTGCTGGCTGCCCTGCCGGGGCTGGGCCACCTGCGCCAGAACTACATGGAGCAGGCCAGCCCCAAACT
GCTGGAGGGGCTGCAATTCGAGCTCGCCGCCTTGCAAAAGCAGGCTGAGCAACTGGCCGCCCTGCCGCCGCTTAGCCTGT
TTGAGGCCCCCCCCGCCGATGAATTCACCCTGGGCGAACCGGTGCGCAAAGAGCTCGGCGCCCAGCCGCGCAGCGAACTG
CTCTCCCTGCTCAAGCGCTATCCGCAAGAGCTGGAAAACAGCCGCAAGGCGCTGCAACAACAACAGGCGGCTCACCAGGC
AGTGCAACAGGACATCACTCGCATGCTGCAAGCAAGCGGGGAGATGGGGGCGCAGCTCACCGCCGGGCGGCAGGCCGTCA
ACCAGGAGCTGGCTCTCATCCTCGGCAGTCTGGCGCTCTGTCTGGTGCTGGTCGCCCTGCTGTTTGCACTGGTGCAGCGT
CGCTGGCTGGTCAAACCGCTGCTGCGGCTGCGCGGTGCTTTTCTGCAACTTGACGAAACAGGCCAGGCCGAAACCCTGCC
CCCGGGGCGCGAGCGCAACGAACTCAGCGACATAGTCGAGAGCTACAACCGGCTCATCGTTCGCCTGCAACTGGATCAGC
AGCAGAAAGAGGGCCAACTGTCGGCGGTCTCATTGAGCCTGCAGGGCATGGTCAATCAGGTCGAAGAGATCCACCACAGC
ACCCGCACGACCGAGCAGGCGGTGGACGAGAGCGACTTGATGATGAGCGAGTTGAACCAGCTGGCCGGCGAGGTCCATCA
GGTGGCCGCCGAGATAGCGCAGCATGCCCAGCACAACGAACACTCCATGAGCCAGAGCGAGCTGCTGGTAGGGGGCATGT
TGCAGGCCACCGCCCAGACCGGTCTTGCCATCGATGAAAGCGACGTCGCCCTGACCCAGCTCAAGCGTTCGGTGGATGAC
GTCACCGCCATCGTCGACGTGATAGGCCACATCGCCCAGCAAACCAACCTGCTGGCGCTCAATGCGGCCATCGAAGCGGC
CAGGGCCGGCGAGCAGGGGCGCGGTTTTGCGGTGGTGGCCGACGAGGTGCGCCACCTCTCCGCCGATACCCAGCGCTCCC
TTGGCCAGATCACCGAGATCCTGGTGCGACTCACCCAATCCGGCGATCAACTCGGCACTGTGCTGGCGCGGATCACCACA
GAAGCAGCCGCCCAGCGCCAGCAGGCGGAGCAGTTGCGCCAGACCACCCAGGCGGTGAGAGAGATGGCGCGCAGCACAGC
CGTCATCGCCCTGCAAGGGGCGGACAACGCCAAGAGCCAGGAGCACAAGCTGGCCAGCTTCGCCGACCTGATCGCTCGCA
TCAGCCAGCACGCCCGCCAGGGCAGCCGGCTCTCGGTTCAGGTGTCTGAACACATCCATCATCAAGCCAGCCAGATCCCC
CGCATTCTCGGCCATCAGGCCTGA

Upstream 100 bases:

>100_bases
CATGACTTCGCAGTCATGAACCGGATGTCATAAAAACGCCATCTTTCCTGCTTAAGCTCACGGCGACTTCCAGTCCCCTG
GCTCAAGCAGGAATCCTGAC

Downstream 100 bases:

>100_bases
ATCAGCCGCTGGACTCCCCTCCCCAACGCCCTGCCCCCGCGTCCCGACGCGGGGGCATATCTTTTTTTGTGCCACAAAAT
TATCTGTTGCATGCAGGTTA

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: TCP pilus biosynthesis protein tcpI [H]

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MKIAHYASLSSLVLLLVAGVQVGALFHGWQQLDQAEQAQHQHERLQQRLTDTLQGTLRDYLSSGDPLRLAEAQTVRQLAL
GQLAQQDEQLTAPLRALLEKMGERIDGDYLAAGKLSGNSQWLLQNAENELTTQARALLRYGQQGAMVPGTDQASAEAYRK
GAADLLAALPGLGHLRQNYMEQASPKLLEGLQFELAALQKQAEQLAALPPLSLFEAPPADEFTLGEPVRKELGAQPRSEL
LSLLKRYPQELENSRKALQQQQAAHQAVQQDITRMLQASGEMGAQLTAGRQAVNQELALILGSLALCLVLVALLFALVQR
RWLVKPLLRLRGAFLQLDETGQAETLPPGRERNELSDIVESYNRLIVRLQLDQQQKEGQLSAVSLSLQGMVNQVEEIHHS
TRTTEQAVDESDLMMSELNQLAGEVHQVAAEIAQHAQHNEHSMSQSELLVGGMLQATAQTGLAIDESDVALTQLKRSVDD
VTAIVDVIGHIAQQTNLLALNAAIEAARAGEQGRGFAVVADEVRHLSADTQRSLGQITEILVRLTQSGDQLGTVLARITT
EAAAQRQQAEQLRQTTQAVREMARSTAVIALQGADNAKSQEHKLASFADLIARISQHARQGSRLSVQVSEHIHHQASQIP
RILGHQA

Sequences:

>Translated_647_residues
MKIAHYASLSSLVLLLVAGVQVGALFHGWQQLDQAEQAQHQHERLQQRLTDTLQGTLRDYLSSGDPLRLAEAQTVRQLAL
GQLAQQDEQLTAPLRALLEKMGERIDGDYLAAGKLSGNSQWLLQNAENELTTQARALLRYGQQGAMVPGTDQASAEAYRK
GAADLLAALPGLGHLRQNYMEQASPKLLEGLQFELAALQKQAEQLAALPPLSLFEAPPADEFTLGEPVRKELGAQPRSEL
LSLLKRYPQELENSRKALQQQQAAHQAVQQDITRMLQASGEMGAQLTAGRQAVNQELALILGSLALCLVLVALLFALVQR
RWLVKPLLRLRGAFLQLDETGQAETLPPGRERNELSDIVESYNRLIVRLQLDQQQKEGQLSAVSLSLQGMVNQVEEIHHS
TRTTEQAVDESDLMMSELNQLAGEVHQVAAEIAQHAQHNEHSMSQSELLVGGMLQATAQTGLAIDESDVALTQLKRSVDD
VTAIVDVIGHIAQQTNLLALNAAIEAARAGEQGRGFAVVADEVRHLSADTQRSLGQITEILVRLTQSGDQLGTVLARITT
EAAAQRQQAEQLRQTTQAVREMARSTAVIALQGADNAKSQEHKLASFADLIARISQHARQGSRLSVQVSEHIHHQASQIP
RILGHQA
>Mature_647_residues
MKIAHYASLSSLVLLLVAGVQVGALFHGWQQLDQAEQAQHQHERLQQRLTDTLQGTLRDYLSSGDPLRLAEAQTVRQLAL
GQLAQQDEQLTAPLRALLEKMGERIDGDYLAAGKLSGNSQWLLQNAENELTTQARALLRYGQQGAMVPGTDQASAEAYRK
GAADLLAALPGLGHLRQNYMEQASPKLLEGLQFELAALQKQAEQLAALPPLSLFEAPPADEFTLGEPVRKELGAQPRSEL
LSLLKRYPQELENSRKALQQQQAAHQAVQQDITRMLQASGEMGAQLTAGRQAVNQELALILGSLALCLVLVALLFALVQR
RWLVKPLLRLRGAFLQLDETGQAETLPPGRERNELSDIVESYNRLIVRLQLDQQQKEGQLSAVSLSLQGMVNQVEEIHHS
TRTTEQAVDESDLMMSELNQLAGEVHQVAAEIAQHAQHNEHSMSQSELLVGGMLQATAQTGLAIDESDVALTQLKRSVDD
VTAIVDVIGHIAQQTNLLALNAAIEAARAGEQGRGFAVVADEVRHLSADTQRSLGQITEILVRLTQSGDQLGTVLARITT
EAAAQRQQAEQLRQTTQAVREMARSTAVIALQGADNAKSQEHKLASFADLIARISQHARQGSRLSVQVSEHIHHQASQIP
RILGHQA

Specific function: May function as an environmental regulator of TCP biogenesis. Negatively regulates the synthesis of the major pilin subunit of TCP (tcpA) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1787690, Length=328, Percent_Identity=30.1829268292683, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789453, Length=338, Percent_Identity=28.4023668639053, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788194, Length=258, Percent_Identity=32.1705426356589, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1788195, Length=299, Percent_Identity=29.7658862876254, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI2367378, Length=210, Percent_Identity=35.2380952380952, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004089 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 70653; Mature: 70653

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAHYASLSSLVLLLVAGVQVGALFHGWQQLDQAEQAQHQHERLQQRLTDTLQGTLRDY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSGDPLRLAEAQTVRQLALGQLAQQDEQLTAPLRALLEKMGERIDGDYLAAGKLSGNSQ
HCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCH
WLLQNAENELTTQARALLRYGQQGAMVPGTDQASAEAYRKGAADLLAALPGLGHLRQNYM
HHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
EQASPKLLEGLQFELAALQKQAEQLAALPPLSLFEAPPADEFTLGEPVRKELGAQPRSEL
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCHHHHHHCCCCHHHH
LSLLKRYPQELENSRKALQQQQAAHQAVQQDITRMLQASGEMGAQLTAGRQAVNQELALI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LGSLALCLVLVALLFALVQRRWLVKPLLRLRGAFLQLDETGQAETLPPGRERNELSDIVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHH
SYNRLIVRLQLDQQQKEGQLSAVSLSLQGMVNQVEEIHHSTRTTEQAVDESDLMMSELNQ
HHHHHEEEEECCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LAGEVHQVAAEIAQHAQHNEHSMSQSELLVGGMLQATAQTGLAIDESDVALTQLKRSVDD
HHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VTAIVDVIGHIAQQTNLLALNAAIEAARAGEQGRGFAVVADEVRHLSADTQRSLGQITEI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHH
LVRLTQSGDQLGTVLARITTEAAAQRQQAEQLRQTTQAVREMARSTAVIALQGADNAKSQ
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHH
EHKLASFADLIARISQHARQGSRLSVQVSEHIHHQASQIPRILGHQA
HHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIAHYASLSSLVLLLVAGVQVGALFHGWQQLDQAEQAQHQHERLQQRLTDTLQGTLRDY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSGDPLRLAEAQTVRQLALGQLAQQDEQLTAPLRALLEKMGERIDGDYLAAGKLSGNSQ
HCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCH
WLLQNAENELTTQARALLRYGQQGAMVPGTDQASAEAYRKGAADLLAALPGLGHLRQNYM
HHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
EQASPKLLEGLQFELAALQKQAEQLAALPPLSLFEAPPADEFTLGEPVRKELGAQPRSEL
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCHHHHHHCCCCHHHH
LSLLKRYPQELENSRKALQQQQAAHQAVQQDITRMLQASGEMGAQLTAGRQAVNQELALI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LGSLALCLVLVALLFALVQRRWLVKPLLRLRGAFLQLDETGQAETLPPGRERNELSDIVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHH
SYNRLIVRLQLDQQQKEGQLSAVSLSLQGMVNQVEEIHHSTRTTEQAVDESDLMMSELNQ
HHHHHEEEEECCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LAGEVHQVAAEIAQHAQHNEHSMSQSELLVGGMLQATAQTGLAIDESDVALTQLKRSVDD
HHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VTAIVDVIGHIAQQTNLLALNAAIEAARAGEQGRGFAVVADEVRHLSADTQRSLGQITEI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHH
LVRLTQSGDQLGTVLARITTEAAAQRQQAEQLRQTTQAVREMARSTAVIALQGADNAKSQ
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHH
EHKLASFADLIARISQHARQGSRLSVQVSEHIHHQASQIPRILGHQA
HHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8005659 [H]