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The map label for this gene is narQ [H]

Identifier: 145299700

GI number: 145299700

Start: 2971287

End: 2972999

Strand: Direct

Name: narQ [H]

Synonym: ASA_2775

Alternate gene names: 145299700

Gene position: 2971287-2972999 (Clockwise)

Preceding gene: 145299679

Following gene: 145299701

Centisome position: 63.19

GC content: 57.85

Gene sequence:

>1713_bases
ATGGGAAAATGGTTCAAAGTACATTCGGTCAGCAGCGCCATCGGGCGTTCCATGGTGCTCATTCTGTTCATGGCGAGCCT
GATCGCCGTCGTGGCCATGGTGACCCTGTTCTACTCGGTGCCCGATGCCAAGGCGATCAACCTGTCGGGTTCGCTGCGGA
TGCAGGCCTATCGCATGGCCTATGAAATAGAGCAGGGCCAGCCAGTGCTGGGGCGGCTCGATCAGTTCGAACAGACCCTG
CATGCACGCGAGCTGCAAGAGACACAGCGTTGGATTACTCCAGCCTCCCTGCGCCGAACCTATGGCGAGGTATTGCTGCA
ATGGCAGATGATGCGCGAGCACATCGAGAACGCCACCCCCAAACGCTACACCGACAATGCGGAACAGTTTGTCGCGGCCA
TCGATGATTTCGTCAATCAGATGCAGTACCACGTGGAGTTCAAGGTGCGCATGCTGGCATTGGCGGAGGGGCTGGGCCTG
CTCTCCATCATCACCATCGCCTGGTTTACCGTACGCTTCACCCGTCAGCAGGTGGTGGCACCGCTCAACCAGCTGGTCTA
TTGCGCCCGCCAGATCCAGCGCCAGGAGTTCGATCTCACCCTGCCTCCCCATTGCGAGAATGAACTGGGAGAATTGTCGG
CTGCCTTCGTCACCATGGCAGATGAGCTCGGCAAGCTCTATCGGGAGCTGGAAAGCAAGGTAGAAGAGAAAACGGCCAAG
CTGCAACAGGCCAACGACACCCTCTCCTTTCTCTATTCCACCGCCCAGAAGCTGCATGCGGCGCCGCTGAGTCGGCGCAC
TCTGCTCAAACTGCTGGAACGGGCCGCGGCCCATCAGCACATAGACCATATTCGGCTGACCCGCTTTGAACAGAACGCCA
TGCCGGACTACATCACAGGCCGCAAGGGCTGGCCAGGCGATCTCGACTCAGTGGTGAGCTTTCATCTGCAGATGGATGAG
CAGGAGTACGGCCGGCTCGACATGATAAGCACTCACCCCATCGACGAGCGGCTGATGAAAAACTTCTCCATGCTGCTGGC
CCAGGTGCTGCACAAGGATCAGACCCTGCTGCAACATCAGCGGCTGTTGCTGATGGAAGAGAGAGCCGTCATCGCCCGCG
AGCTGCACGATTCGCTAGCCCAGGCGCTCTCCTACCTCAAAATCCAGTCCACTCTGCTCAAACGCTCTTTTGCCAAGGGC
GATAACGCAAAGGCAGAGGCGGCCATGCAGCAGATAGACGAGGGACTCAGCAGCGCCTACACCCAGTTGCGCGAGCTGCT
GGGCACTTTCCGCCTCAGCATCGGCGATGCCAATCTGGGGGAAGCTATCAAGGTGATGCTGGAGCAGCTCAAGCCTCAGA
CCCAGGCCGAGATCAGGCTCCATTACGGTCTGGCTGATACGGATCTTGAAGCGGGTCAGCACATTCATATTCTGCAACTG
ATCCGTGAAGCAGTGCTCAATGCCATCAAACATGCCAGCGCGCAAGTGATTGATGTTAGCTGTGAGACCCTCGCCGATGG
TAACATTCAGGTTCAGATTTCAGATGACGGTGTTGGAATAGGGATCGCAAGCTCGGCGGTCAACCACTATGGCCTCAGCA
TCATGAATGAGCGTGCCAGCAAGCTGCATGGTCTGCTGACCATCAGCGAACAGCCGCCCAGAGGCACCTGCGTACATCTG
ACCTTTCCCACCAGCCTAACAAGAGACGCCTGA

Upstream 100 bases:

>100_bases
AGCTCGGAGTTCATCTAGTTTTTCGATGCTAGCACAAGTTGGAGTACACTCCCACGCAACTTTATGGGGTTATTGCCAGA
GGTCGGGGACGCCAGCCAGC

Downstream 100 bases:

>100_bases
TGGACGAGATGAAATATAGTGTTCTGGTCGTGGATGACCATCCCCTCATGCGCAAAGGGATTGTGCAACTGCTGGCCCTG
GAAGAGAACATAGAGGTGAT

Product: nitrate/nitrite sensor protein NarQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MGKWFKVHSVSSAIGRSMVLILFMASLIAVVAMVTLFYSVPDAKAINLSGSLRMQAYRMAYEIEQGQPVLGRLDQFEQTL
HARELQETQRWITPASLRRTYGEVLLQWQMMREHIENATPKRYTDNAEQFVAAIDDFVNQMQYHVEFKVRMLALAEGLGL
LSIITIAWFTVRFTRQQVVAPLNQLVYCARQIQRQEFDLTLPPHCENELGELSAAFVTMADELGKLYRELESKVEEKTAK
LQQANDTLSFLYSTAQKLHAAPLSRRTLLKLLERAAAHQHIDHIRLTRFEQNAMPDYITGRKGWPGDLDSVVSFHLQMDE
QEYGRLDMISTHPIDERLMKNFSMLLAQVLHKDQTLLQHQRLLLMEERAVIARELHDSLAQALSYLKIQSTLLKRSFAKG
DNAKAEAAMQQIDEGLSSAYTQLRELLGTFRLSIGDANLGEAIKVMLEQLKPQTQAEIRLHYGLADTDLEAGQHIHILQL
IREAVLNAIKHASAQVIDVSCETLADGNIQVQISDDGVGIGIASSAVNHYGLSIMNERASKLHGLLTISEQPPRGTCVHL
TFPTSLTRDA

Sequences:

>Translated_570_residues
MGKWFKVHSVSSAIGRSMVLILFMASLIAVVAMVTLFYSVPDAKAINLSGSLRMQAYRMAYEIEQGQPVLGRLDQFEQTL
HARELQETQRWITPASLRRTYGEVLLQWQMMREHIENATPKRYTDNAEQFVAAIDDFVNQMQYHVEFKVRMLALAEGLGL
LSIITIAWFTVRFTRQQVVAPLNQLVYCARQIQRQEFDLTLPPHCENELGELSAAFVTMADELGKLYRELESKVEEKTAK
LQQANDTLSFLYSTAQKLHAAPLSRRTLLKLLERAAAHQHIDHIRLTRFEQNAMPDYITGRKGWPGDLDSVVSFHLQMDE
QEYGRLDMISTHPIDERLMKNFSMLLAQVLHKDQTLLQHQRLLLMEERAVIARELHDSLAQALSYLKIQSTLLKRSFAKG
DNAKAEAAMQQIDEGLSSAYTQLRELLGTFRLSIGDANLGEAIKVMLEQLKPQTQAEIRLHYGLADTDLEAGQHIHILQL
IREAVLNAIKHASAQVIDVSCETLADGNIQVQISDDGVGIGIASSAVNHYGLSIMNERASKLHGLLTISEQPPRGTCVHL
TFPTSLTRDA
>Mature_569_residues
GKWFKVHSVSSAIGRSMVLILFMASLIAVVAMVTLFYSVPDAKAINLSGSLRMQAYRMAYEIEQGQPVLGRLDQFEQTLH
ARELQETQRWITPASLRRTYGEVLLQWQMMREHIENATPKRYTDNAEQFVAAIDDFVNQMQYHVEFKVRMLALAEGLGLL
SIITIAWFTVRFTRQQVVAPLNQLVYCARQIQRQEFDLTLPPHCENELGELSAAFVTMADELGKLYRELESKVEEKTAKL
QQANDTLSFLYSTAQKLHAAPLSRRTLLKLLERAAAHQHIDHIRLTRFEQNAMPDYITGRKGWPGDLDSVVSFHLQMDEQ
EYGRLDMISTHPIDERLMKNFSMLLAQVLHKDQTLLQHQRLLLMEERAVIARELHDSLAQALSYLKIQSTLLKRSFAKGD
NAKAEAAMQQIDEGLSSAYTQLRELLGTFRLSIGDANLGEAIKVMLEQLKPQTQAEIRLHYGLADTDLEAGQHIHILQLI
REAVLNAIKHASAQVIDVSCETLADGNIQVQISDDGVGIGIASSAVNHYGLSIMNERASKLHGLLTISEQPPRGTCVHLT
FPTSLTRDA

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate/nitrite availability to the narL/narP proteins. NarQ probably activates narL and narP by phosphorylation. NarQ probably negatively regulates the narL protein by dephosphorylation [H]

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=561, Percent_Identity=40.6417112299465, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1787474, Length=589, Percent_Identity=30.2207130730051, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI87082311, Length=224, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 64351; Mature: 64220

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50885 HAMP ; PS00041 HTH_ARAC_FAMILY_1 ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKWFKVHSVSSAIGRSMVLILFMASLIAVVAMVTLFYSVPDAKAINLSGSLRMQAYRMA
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHH
YEIEQGQPVLGRLDQFEQTLHARELQETQRWITPASLRRTYGEVLLQWQMMREHIENATP
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
KRYTDNAEQFVAAIDDFVNQMQYHVEFKVRMLALAEGLGLLSIITIAWFTVRFTRQQVVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
PLNQLVYCARQIQRQEFDLTLPPHCENELGELSAAFVTMADELGKLYRELESKVEEKTAK
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQQANDTLSFLYSTAQKLHAAPLSRRTLLKLLERAAAHQHIDHIRLTRFEQNAMPDYITG
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RKGWPGDLDSVVSFHLQMDEQEYGRLDMISTHPIDERLMKNFSMLLAQVLHKDQTLLQHQ
CCCCCCCHHHHHHHHHCCCHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLLMEERAVIARELHDSLAQALSYLKIQSTLLKRSFAKGDNAKAEAAMQQIDEGLSSAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
TQLRELLGTFRLSIGDANLGEAIKVMLEQLKPQTQAEIRLHYGLADTDLEAGQHIHILQL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCCHHHHHH
IREAVLNAIKHASAQVIDVSCETLADGNIQVQISDDGVGIGIASSAVNHYGLSIMNERAS
HHHHHHHHHHHCCCEEEEEEHHHCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHH
KLHGLLTISEQPPRGTCVHLTFPTSLTRDA
HHHHEEEECCCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
GKWFKVHSVSSAIGRSMVLILFMASLIAVVAMVTLFYSVPDAKAINLSGSLRMQAYRMA
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHH
YEIEQGQPVLGRLDQFEQTLHARELQETQRWITPASLRRTYGEVLLQWQMMREHIENATP
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
KRYTDNAEQFVAAIDDFVNQMQYHVEFKVRMLALAEGLGLLSIITIAWFTVRFTRQQVVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
PLNQLVYCARQIQRQEFDLTLPPHCENELGELSAAFVTMADELGKLYRELESKVEEKTAK
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQQANDTLSFLYSTAQKLHAAPLSRRTLLKLLERAAAHQHIDHIRLTRFEQNAMPDYITG
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RKGWPGDLDSVVSFHLQMDEQEYGRLDMISTHPIDERLMKNFSMLLAQVLHKDQTLLQHQ
CCCCCCCHHHHHHHHHCCCHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLLMEERAVIARELHDSLAQALSYLKIQSTLLKRSFAKGDNAKAEAAMQQIDEGLSSAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
TQLRELLGTFRLSIGDANLGEAIKVMLEQLKPQTQAEIRLHYGLADTDLEAGQHIHILQL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCCHHHHHH
IREAVLNAIKHASAQVIDVSCETLADGNIQVQISDDGVGIGIASSAVNHYGLSIMNERAS
HHHHHHHHHHHCCCEEEEEEHHHCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHH
KLHGLLTISEQPPRGTCVHLTFPTSLTRDA
HHHHEEEECCCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1528845; 1508040; 9205837; 9278503 [H]