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The map label for this gene is gdhA [H]

Identifier: 145299657

GI number: 145299657

Start: 2928087

End: 2929433

Strand: Direct

Name: gdhA [H]

Synonym: ASA_2731

Alternate gene names: 145299657

Gene position: 2928087-2929433 (Clockwise)

Preceding gene: 145299655

Following gene: 145299658

Centisome position: 62.27

GC content: 60.95

Gene sequence:

>1347_bases
ATGAGTCATTCCCTATCCCAGGCGGATTTTCTGGCCCAGGTGGCCAGCCGCAATCCCCATCAACCGGAATTTATTCAGGC
AGTGAGCGAGGTGATCGCCTCCATCTGGCCTTACGTGGAACGTCACCCCCGCTACCTGCAACAGGGCCTGCTGGAGCGGC
TGGTGGAGCCGGAGCGACTGATCCAGTTTCGGATAAGCTGGGTCGATGACAAGGGTAAGGTGCAAGTCAATCGCGGCTAT
CGCATCCAGCACAGCTCGGCTATCGGCCCCTTCAAGGGGGGCATGCGTTTTCATCCCTCAGTGGATCTCTCTGTGCTCAA
GTTCCTCGCCTTCGAGCAGACCTTCAAGAACGCCCTCACTACCCTGCCCATGGGTGGCGGCAAGGGCGGCAGCGATTTTG
ACCCCAAGGGCAAGAGCGAGGGGGAAGTGATGCGCTTCTGTCAGGCGCTGATGCTGGAGCTCTACCGTCATCTGGGGGCC
AATACCGATGTGCCTGCCGGCGATATCGGGGTGGGCGGGCGCGAGGTGAGTTACATGGCAGGCATGATGAAGAAGCTCAC
CAACAGCGCCGAATGTATCTTCACCGGCAAGGGGCTCTCATTTGGGGGCAGCCTCATTCGTCCCGAGGCCACCGGCTACG
GCCTGCTCTATTTTGTCGAGGCCATGCTGGCCAACCGTGACCAGTCGCTGGCGGGCATGACTGTCTCTGTGTCGGGATCC
GGCAACGTGGCGCAATATGCCATCGAGAAAGCGATGGCGCTGGGTGCCAAGGTGATCACTGCCTCCGACTCGGGTGGCAC
AGTCCATGATCCTGACGGTTTCACCCCCGACAAGCTCGCCAGATTGATGGAACTCAAGAACGAGCAACGCCAGCGTATCG
CCGATTATGCCCATGATCAGGGGCTGGAGTATTTCAGTGGCCGCACGCCCTGGCACCTGCCAACCCAGATAGCGCTGCCC
TGCGCCACCCAGAACGAATTGGGGGAAGAGGACGCCAGACAACTGGTGGCCAACGGCGTACAACTGGTGGCCGAGGGCGC
CAACATGCCGAGCTGCGCCGCCGCCATCAAAGTCTTTCATGAGGCCAAGGTGCTGTTTGCCCCAGGCAAGGCGGCCAATG
CAGGGGGCGTCGCCACCTCTGGGCTTGAGATGAGCCAGAATGCCCAGCGCCTCTCCTGGACACGGGAGGAGGTGGATGCC
CGCCTCAAGGGGATCATGACCAGCATACACGCCACCTGTGTGCGTTACGGTGAAGAGGAGCAGGGCCACGTGAACTATGA
GAGCGGCGCCAATATCGCCGGTTTCGTCAAGGTGGCCGATGCCATTCTGGCACAGGGAGTCTGCTAG

Upstream 100 bases:

>100_bases
TGTCCGCATTCATCCCCAACACAAATCATAAATTTGGATGCACGGCATGCGCTTTTCTCTGGCAAGAGGCCAATTTCATC
ATTCACTACAGGAGTTACAG

Downstream 100 bases:

>100_bases
TCCCGGCAAGGATGTGCCATCAGCAAGCATTGACCACGACGTGCAACACCAGACGGCAGCCCATGGGCTGCCGTCTTTTC
ATGGTCTTGACGAGCGCCCA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSHSLSQADFLAQVASRNPHQPEFIQAVSEVIASIWPYVERHPRYLQQGLLERLVEPERLIQFRISWVDDKGKVQVNRGY
RIQHSSAIGPFKGGMRFHPSVDLSVLKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMLELYRHLGA
NTDVPAGDIGVGGREVSYMAGMMKKLTNSAECIFTGKGLSFGGSLIRPEATGYGLLYFVEAMLANRDQSLAGMTVSVSGS
GNVAQYAIEKAMALGAKVITASDSGGTVHDPDGFTPDKLARLMELKNEQRQRIADYAHDQGLEYFSGRTPWHLPTQIALP
CATQNELGEEDARQLVANGVQLVAEGANMPSCAAAIKVFHEAKVLFAPGKAANAGGVATSGLEMSQNAQRLSWTREEVDA
RLKGIMTSIHATCVRYGEEEQGHVNYESGANIAGFVKVADAILAQGVC

Sequences:

>Translated_448_residues
MSHSLSQADFLAQVASRNPHQPEFIQAVSEVIASIWPYVERHPRYLQQGLLERLVEPERLIQFRISWVDDKGKVQVNRGY
RIQHSSAIGPFKGGMRFHPSVDLSVLKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMLELYRHLGA
NTDVPAGDIGVGGREVSYMAGMMKKLTNSAECIFTGKGLSFGGSLIRPEATGYGLLYFVEAMLANRDQSLAGMTVSVSGS
GNVAQYAIEKAMALGAKVITASDSGGTVHDPDGFTPDKLARLMELKNEQRQRIADYAHDQGLEYFSGRTPWHLPTQIALP
CATQNELGEEDARQLVANGVQLVAEGANMPSCAAAIKVFHEAKVLFAPGKAANAGGVATSGLEMSQNAQRLSWTREEVDA
RLKGIMTSIHATCVRYGEEEQGHVNYESGANIAGFVKVADAILAQGVC
>Mature_447_residues
SHSLSQADFLAQVASRNPHQPEFIQAVSEVIASIWPYVERHPRYLQQGLLERLVEPERLIQFRISWVDDKGKVQVNRGYR
IQHSSAIGPFKGGMRFHPSVDLSVLKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMLELYRHLGAN
TDVPAGDIGVGGREVSYMAGMMKKLTNSAECIFTGKGLSFGGSLIRPEATGYGLLYFVEAMLANRDQSLAGMTVSVSGSG
NVAQYAIEKAMALGAKVITASDSGGTVHDPDGFTPDKLARLMELKNEQRQRIADYAHDQGLEYFSGRTPWHLPTQIALPC
ATQNELGEEDARQLVANGVQLVAEGANMPSCAAAIKVFHEAKVLFAPGKAANAGGVATSGLEMSQNAQRLSWTREEVDAR
LKGIMTSIHATCVRYGEEEQGHVNYESGANIAGFVKVADAILAQGVC

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=372, Percent_Identity=31.1827956989247, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4885281, Length=372, Percent_Identity=30.3763440860215, Blast_Score=149, Evalue=4e-36,
Organism=Escherichia coli, GI1788059, Length=447, Percent_Identity=70.4697986577181, Blast_Score=654, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544676, Length=371, Percent_Identity=27.7628032345013, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324951, Length=451, Percent_Identity=49.6674057649667, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6319256, Length=453, Percent_Identity=49.2273730684327, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45549226, Length=362, Percent_Identity=30.939226519337, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI45553475, Length=362, Percent_Identity=30.939226519337, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24649283, Length=372, Percent_Identity=29.8387096774194, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24649460, Length=296, Percent_Identity=31.0810810810811, Blast_Score=120, Evalue=3e-27,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 48458; Mature: 48326

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSLSQADFLAQVASRNPHQPEFIQAVSEVIASIWPYVERHPRYLQQGLLERLVEPERL
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHH
IQFRISWVDDKGKVQVNRGYRIQHSSAIGPFKGGMRFHPSVDLSVLKFLAFEQTFKNALT
HHHHHEEECCCCCEEECCCEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHH
TLPMGGGKGGSDFDPKGKSEGEVMRFCQALMLELYRHLGANTDVPAGDIGVGGREVSYMA
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
GMMKKLTNSAECIFTGKGLSFGGSLIRPEATGYGLLYFVEAMLANRDQSLAGMTVSVSGS
HHHHHHCCCCCEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCC
GNVAQYAIEKAMALGAKVITASDSGGTVHDPDGFTPDKLARLMELKNEQRQRIADYAHDQ
CCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GLEYFSGRTPWHLPTQIALPCATQNELGEEDARQLVANGVQLVAEGANMPSCAAAIKVFH
CCHHHCCCCCCCCCCCEEECCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
EAKVLFAPGKAANAGGVATSGLEMSQNAQRLSWTREEVDARLKGIMTSIHATCVRYGEEE
HCEEEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QGHVNYESGANIAGFVKVADAILAQGVC
CCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SHSLSQADFLAQVASRNPHQPEFIQAVSEVIASIWPYVERHPRYLQQGLLERLVEPERL
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHH
IQFRISWVDDKGKVQVNRGYRIQHSSAIGPFKGGMRFHPSVDLSVLKFLAFEQTFKNALT
HHHHHEEECCCCCEEECCCEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHH
TLPMGGGKGGSDFDPKGKSEGEVMRFCQALMLELYRHLGANTDVPAGDIGVGGREVSYMA
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
GMMKKLTNSAECIFTGKGLSFGGSLIRPEATGYGLLYFVEAMLANRDQSLAGMTVSVSGS
HHHHHHCCCCCEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCC
GNVAQYAIEKAMALGAKVITASDSGGTVHDPDGFTPDKLARLMELKNEQRQRIADYAHDQ
CCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GLEYFSGRTPWHLPTQIALPCATQNELGEEDARQLVANGVQLVAEGANMPSCAAAIKVFH
CCHHHCCCCCCCCCCCEEECCCCCHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
EAKVLFAPGKAANAGGVATSGLEMSQNAQRLSWTREEVDARLKGIMTSIHATCVRYGEEE
HCEEEECCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QGHVNYESGANIAGFVKVADAILAQGVC
CCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308576; 6373501; 9097039; 9278503; 9298646 [H]