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The map label for this gene is btuF [H]

Identifier: 145299584

GI number: 145299584

Start: 2840337

End: 2841143

Strand: Direct

Name: btuF [H]

Synonym: ASA_2655

Alternate gene names: 145299584

Gene position: 2840337-2841143 (Clockwise)

Preceding gene: 145299583

Following gene: 145299591

Centisome position: 60.4

GC content: 58.24

Gene sequence:

>807_bases
ATGGCTAACTATTTCGCCCTGCTCTGCTGTTCGCTGACGCTGCAGGCTGCAACACCGATGCGCATCGTCAGTCTGACCCC
CCATTTGACCGAGCTGTTGTTCCTCGTCGGGGCAGGCGATCGCATCGTCGCCACCGATGATGCATCGGATTTTCCGCCAG
AAGTGAACGATCGCCCGCACGTTGCCAACTATCGCAGCATCAACCTTGAAGCCCTGCTGGCCCAGAAACCGGATCTGGTG
GTGGCCTGGCGCTCTGCCCAATCCCGTATGCTGGCACCTGTCGAACAGCTGGGGATCCCTGTGTTCTACTCTGAGCCAAC
TGATTTTGCCTCCCTGGCCACCGAAATTCGGGCGCTGGGAGCACTGCTGGACCTGGAGCAGACAGCGAACACTCAGGCCG
AGGCCTACCTCGCCAGATTGGCGGCGCTCAAGCAACGTTATGGTCAGCCCAACCATATCAAGGTGTTTTATCAGCTCTGG
TATCCGCCCCTGACCAGCGTCAGCGGCTCGGCGTGGCCAGCCCAGGCCATCGAACTGTGCGGTGCAGAGAACCTGATGGC
TGGCGCCAGAACCCCTTACCCTCAGGTCAATTTGGAGCAGGTGATCAAATCTGACCCTGCGCTCATTCTGGCTGGTAGCC
AGGATCCTGACGCTTTACGTCACTGGCAACAATGGCCCATGGTCGATGCGGTCAAACATCAACGATTGCAACTCATCAAT
TCGGATGAACTGCACCGCTTCACCCCGAGGGCGCTCAATGCCGTGGAGCAGATATGCAATGCCATCGCTCGCGTTCAAGC
AAAATAG

Upstream 100 bases:

>100_bases
CCGGCAGCCGCCACTGCGCTGCGCGTACTCAACCGGTTGCAACTGATTGGCTGGGGTTGGCTCGCACTGGCCGCACTGCT
CACAGTGCTGGAGCAGCTCC

Downstream 100 bases:

>100_bases
CCACTGGCAATAACGCAACGATATTGAAAACAGAGACGCCGGCATCCGCCGGCGTCTTGTTGTGTCTGAAAACTATCTCT
TCACTGAAAGGCATTACTTG

Product: cobalamin binding protein BtuF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MANYFALLCCSLTLQAATPMRIVSLTPHLTELLFLVGAGDRIVATDDASDFPPEVNDRPHVANYRSINLEALLAQKPDLV
VAWRSAQSRMLAPVEQLGIPVFYSEPTDFASLATEIRALGALLDLEQTANTQAEAYLARLAALKQRYGQPNHIKVFYQLW
YPPLTSVSGSAWPAQAIELCGAENLMAGARTPYPQVNLEQVIKSDPALILAGSQDPDALRHWQQWPMVDAVKHQRLQLIN
SDELHRFTPRALNAVEQICNAIARVQAK

Sequences:

>Translated_268_residues
MANYFALLCCSLTLQAATPMRIVSLTPHLTELLFLVGAGDRIVATDDASDFPPEVNDRPHVANYRSINLEALLAQKPDLV
VAWRSAQSRMLAPVEQLGIPVFYSEPTDFASLATEIRALGALLDLEQTANTQAEAYLARLAALKQRYGQPNHIKVFYQLW
YPPLTSVSGSAWPAQAIELCGAENLMAGARTPYPQVNLEQVIKSDPALILAGSQDPDALRHWQQWPMVDAVKHQRLQLIN
SDELHRFTPRALNAVEQICNAIARVQAK
>Mature_267_residues
ANYFALLCCSLTLQAATPMRIVSLTPHLTELLFLVGAGDRIVATDDASDFPPEVNDRPHVANYRSINLEALLAQKPDLVV
AWRSAQSRMLAPVEQLGIPVFYSEPTDFASLATEIRALGALLDLEQTANTQAEAYLARLAALKQRYGQPNHIKVFYQLWY
PPLTSVSGSAWPAQAIELCGAENLMAGARTPYPQVNLEQVIKSDPALILAGSQDPDALRHWQQWPMVDAVKHQRLQLINS
DELHRFTPRALNAVEQICNAIARVQAK

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=250, Percent_Identity=32.8, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 29648; Mature: 29517

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANYFALLCCSLTLQAATPMRIVSLTPHLTELLFLVGAGDRIVATDDASDFPPEVNDRPH
CCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCC
VANYRSINLEALLAQKPDLVVAWRSAQSRMLAPVEQLGIPVFYSEPTDFASLATEIRALG
CCCCCCCCCEEEECCCCCEEEEECCCCCHHHCCHHHHCCCEEECCCCHHHHHHHHHHHHH
ALLDLEQTANTQAEAYLARLAALKQRYGQPNHIKVFYQLWYPPLTSVSGSAWPAQAIELC
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHH
GAENLMAGARTPYPQVNLEQVIKSDPALILAGSQDPDALRHWQQWPMVDAVKHQRLQLIN
CCHHHHCCCCCCCCCCCHHHHHCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCC
SDELHRFTPRALNAVEQICNAIARVQAK
CCCHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ANYFALLCCSLTLQAATPMRIVSLTPHLTELLFLVGAGDRIVATDDASDFPPEVNDRPH
CHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCC
VANYRSINLEALLAQKPDLVVAWRSAQSRMLAPVEQLGIPVFYSEPTDFASLATEIRALG
CCCCCCCCCEEEECCCCCEEEEECCCCCHHHCCHHHHCCCEEECCCCHHHHHHHHHHHHH
ALLDLEQTANTQAEAYLARLAALKQRYGQPNHIKVFYQLWYPPLTSVSGSAWPAQAIELC
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHH
GAENLMAGARTPYPQVNLEQVIKSDPALILAGSQDPDALRHWQQWPMVDAVKHQRLQLIN
CCHHHHCCCCCCCCCCCHHHHHCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCC
SDELHRFTPRALNAVEQICNAIARVQAK
CCCHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]