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The map label for this gene is cbiB [H]

Identifier: 145299583

GI number: 145299583

Start: 2839421

End: 2840344

Strand: Direct

Name: cbiB [H]

Synonym: ASA_2654

Alternate gene names: 145299583

Gene position: 2839421-2840344 (Clockwise)

Preceding gene: 145299582

Following gene: 145299584

Centisome position: 60.38

GC content: 65.69

Gene sequence:

>924_bases
ATGGCCGCCACTCCGGCGGCCATTTTGCTCGGGGCCGTGCTGCTGGAAGCCATACTGCCCTGGCCTGCCCAGTTCAGACT
CTCTGCCCTGGTACCCCTGTTGACCCGCCTGGGCCACAAGGTTCACCGCCCCGATGGCTCCCCTCAGCAACAGTTTCGGG
CCGGCCTGCTGGCCCTGCTGGTCGTCTGGCTGCCATGCGCAGCCGGACTCTGGGCGGTGCGCAATCTCTCCCTCTCCGAG
CCGCTGTTTGACCTGTTGTTTCTGTTGCTGATGATAGAGTCCCGTCCGTTGCGTGAGCTGGCCAAGGCAACCCGTTCCCT
GGCAAGCCAGGAGACGCTGGTCGTTGCCCGCCTGCAGGCAACCCCCTGGCTCAGGCGCGATACCGGCAAGCTCTCCGTGA
TGGGGATTGCCAAGGCCGTCAGCGAGACCTGTGCACTGCGCCTGGCAGGACAATGGGCGGGTCCCTTGCTGGGGTTTGCA
CTGGCAGGCGTTCAGGGCGCCCTGCTGTGGCGACTCGCCCAATTGATGGATCAGGGCTGGAGCCCCAAGCAGGTGGCATT
CAGCCACTTCGGCCGCGCCGCCGCCGTGCTTTATCAGGCCCTCTGCACCCCTGCCATTCTGCTGCTGGCACTGCCCCTGT
GCCTGACCCAAGTGCGCCAGCTGGGCGCCAGGATGCGCACTGCCATTCAGTGGCCCTACCCCGCCATGGGCATCTTGCTC
GCCCAGCTATCCAGCTCGCTGGGCATCCGGCTGGGTGGCCCCCGCTATTATCAGGACACCCTGTTCCGCTTCCCGGTATT
TGATGCACCCAGCGACCCGGCAGCCGCCACTGCGCTGCGCGTACTCAACCGGTTGCAACTGATTGGCTGGGGTTGGCTCG
CACTGGCCGCACTGCTCACAGTGCTGGAGCAGCTCCATGGCTAA

Upstream 100 bases:

>100_bases
AATCCTTCGATGCTTTCATCGAGGTGGCGGGCCAGCATTCTGCCGAGATGATCATCAAGATGCTCGGCAAACTGTGATCG
ACCTCGCCACACTGGAACAC

Downstream 100 bases:

>100_bases
CTATTTCGCCCTGCTCTGCTGTTCGCTGACGCTGCAGGCTGCAACACCGATGCGCATCGTCAGTCTGACCCCCCATTTGA
CCGAGCTGTTGTTCCTCGTC

Product: cobalamin biosynthesis protein CbiB

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MAATPAAILLGAVLLEAILPWPAQFRLSALVPLLTRLGHKVHRPDGSPQQQFRAGLLALLVVWLPCAAGLWAVRNLSLSE
PLFDLLFLLLMIESRPLRELAKATRSLASQETLVVARLQATPWLRRDTGKLSVMGIAKAVSETCALRLAGQWAGPLLGFA
LAGVQGALLWRLAQLMDQGWSPKQVAFSHFGRAAAVLYQALCTPAILLLALPLCLTQVRQLGARMRTAIQWPYPAMGILL
AQLSSSLGIRLGGPRYYQDTLFRFPVFDAPSDPAAATALRVLNRLQLIGWGWLALAALLTVLEQLHG

Sequences:

>Translated_307_residues
MAATPAAILLGAVLLEAILPWPAQFRLSALVPLLTRLGHKVHRPDGSPQQQFRAGLLALLVVWLPCAAGLWAVRNLSLSE
PLFDLLFLLLMIESRPLRELAKATRSLASQETLVVARLQATPWLRRDTGKLSVMGIAKAVSETCALRLAGQWAGPLLGFA
LAGVQGALLWRLAQLMDQGWSPKQVAFSHFGRAAAVLYQALCTPAILLLALPLCLTQVRQLGARMRTAIQWPYPAMGILL
AQLSSSLGIRLGGPRYYQDTLFRFPVFDAPSDPAAATALRVLNRLQLIGWGWLALAALLTVLEQLHG
>Mature_306_residues
AATPAAILLGAVLLEAILPWPAQFRLSALVPLLTRLGHKVHRPDGSPQQQFRAGLLALLVVWLPCAAGLWAVRNLSLSEP
LFDLLFLLLMIESRPLRELAKATRSLASQETLVVARLQATPWLRRDTGKLSVMGIAKAVSETCALRLAGQWAGPLLGFAL
AGVQGALLWRLAQLMDQGWSPKQVAFSHFGRAAAVLYQALCTPAILLLALPLCLTQVRQLGARMRTAIQWPYPAMGILLA
QLSSSLGIRLGGPRYYQDTLFRFPVFDAPSDPAAATALRVLNRLQLIGWGWLALAALLTVLEQLHG

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 33349; Mature: 33218

Theoretical pI: Translated: 11.14; Mature: 11.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATPAAILLGAVLLEAILPWPAQFRLSALVPLLTRLGHKVHRPDGSPQQQFRAGLLALL
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHHHHH
VVWLPCAAGLWAVRNLSLSEPLFDLLFLLLMIESRPLRELAKATRSLASQETLVVARLQA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
TPWLRRDTGKLSVMGIAKAVSETCALRLAGQWAGPLLGFALAGVQGALLWRLAQLMDQGW
CCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SPKQVAFSHFGRAAAVLYQALCTPAILLLALPLCLTQVRQLGARMRTAIQWPYPAMGILL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AQLSSSLGIRLGGPRYYQDTLFRFPVFDAPSDPAAATALRVLNRLQLIGWGWLALAALLT
HHHHHHCCEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLEQLHG
HHHHHCC
>Mature Secondary Structure 
AATPAAILLGAVLLEAILPWPAQFRLSALVPLLTRLGHKVHRPDGSPQQQFRAGLLALL
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHHHHH
VVWLPCAAGLWAVRNLSLSEPLFDLLFLLLMIESRPLRELAKATRSLASQETLVVARLQA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
TPWLRRDTGKLSVMGIAKAVSETCALRLAGQWAGPLLGFALAGVQGALLWRLAQLMDQGW
CCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SPKQVAFSHFGRAAAVLYQALCTPAILLLALPLCLTQVRQLGARMRTAIQWPYPAMGILL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AQLSSSLGIRLGGPRYYQDTLFRFPVFDAPSDPAAATALRVLNRLQLIGWGWLALAALLT
HHHHHHCCEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLEQLHG
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]