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The map label for this gene is nemA [H]

Identifier: 145299443

GI number: 145299443

Start: 2672733

End: 2673854

Strand: Direct

Name: nemA [H]

Synonym: ASA_2505

Alternate gene names: 145299443

Gene position: 2672733-2673854 (Clockwise)

Preceding gene: 145299442

Following gene: 145299445

Centisome position: 56.84

GC content: 63.46

Gene sequence:

>1122_bases
ATGAGCCACGACAACCTGTTCTGCCCGCTGCGTCTGGGCCAGTTGCACCTTAACAACCGCATCGTCATGCCCCCCATGAC
CCGCTCCCGTGCCAGCCAGCCCGGTGACGTGGCCAACGCCATCATGGCCACTTATTACGCCCAGCGCGCCGAGGCCGGCC
TCATCATCAGCGAAGGCACCCAGATAGACCCGCTGGGCAAAGGCTACGCCTGGACCCCCGGGATCCACAGCGCCGAGCAG
GTAGCGGGCTGGAAGGGGGTGACCGATGCAGTCCACGCCAAGGGGGGCACCATCTTTGCCCAGCTCTGGCACGTGGGCCG
CGTCACGCATACTGACAATATCGGCGGGGCCCAGTCCATCTCTTCATCTGCCATCAGAGCCGAAGGGGTGCGGGTATTTG
TCGATAACGGCAGCGATGCTCCCGGTTTTGTCGAGACCACAACCCCGCGCGCCATGACCCAGAGCGACATAGATGCGGTA
GTGGGCCAGTTCCGCCAGGCGGCTCGCAATGCCCTAAACGCCGGCTTTGACGGCATAGAGCTGCACGCTGCCAACGGTTA
CCTCATCAACCAGTTCCTCGACTCCGAGTCCAATACCCGCACCGACAACTACGGCGGCTCGCTGGAAAATCGTCTGCGTT
TCCTCAAAGAGGTGACACAAGCGGTCAGTGCCGAAATCGGGGCGGACAGGGTTGGGGTGCGCCTTGCGCCGCTCACCACC
CTCAACGGCACCGTTGATGCCAACCCGCAAGAGACCTATCTGGCCGCCGCTCGCTTGCTGGGTGAACTGGACGTCACCTA
TCTGCATATCGCCGAAGCGGACTGGGACGATGCGCCCCTGATGGCGGAAAACTTCAAGCAGGGGTTGCGAGATGCCTTCC
CCAACACCCTCATCTATGCGGGCAAGTATGACGGTGAGCGGGCGCGTGCCGCCCTCAAGGCGGGCTGGGCCGACATGATT
GGCTTTGGTCGTCCCTTCGTGGCCAACCCGGATCTGCCGAACCGGTTGCGCCACGGCTACCCCCTCGCGCCCCATGATCC
GGCGACCCTGTTTGGCGGCGGTGAGAAGGGACTGACCGACTACCCGACCTATCAGGCGCTAGCCGAGACGCAATCAGCCT
GA

Upstream 100 bases:

>100_bases
GATTGCTGCATCAAGTGCAATTCTTTTCTGCAATTACCCCTCTTTTTCACAAATAAAAGCAGGACTAGAGTGACCTCAAT
CCCACCATGAGGAACACAAA

Downstream 100 bases:

>100_bases
GACTGGCAAGCCGCCCACCTCGGTCACAAAGCCGCACAGTAAAACGCGCCCGCTATTGATGCGGGCGCGTTTTTTGGGGT
GTGGCGTCAGCGAGCCAGAC

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSHDNLFCPLRLGQLHLNNRIVMPPMTRSRASQPGDVANAIMATYYAQRAEAGLIISEGTQIDPLGKGYAWTPGIHSAEQ
VAGWKGVTDAVHAKGGTIFAQLWHVGRVTHTDNIGGAQSISSSAIRAEGVRVFVDNGSDAPGFVETTTPRAMTQSDIDAV
VGQFRQAARNALNAGFDGIELHAANGYLINQFLDSESNTRTDNYGGSLENRLRFLKEVTQAVSAEIGADRVGVRLAPLTT
LNGTVDANPQETYLAAARLLGELDVTYLHIAEADWDDAPLMAENFKQGLRDAFPNTLIYAGKYDGERARAALKAGWADMI
GFGRPFVANPDLPNRLRHGYPLAPHDPATLFGGGEKGLTDYPTYQALAETQSA

Sequences:

>Translated_373_residues
MSHDNLFCPLRLGQLHLNNRIVMPPMTRSRASQPGDVANAIMATYYAQRAEAGLIISEGTQIDPLGKGYAWTPGIHSAEQ
VAGWKGVTDAVHAKGGTIFAQLWHVGRVTHTDNIGGAQSISSSAIRAEGVRVFVDNGSDAPGFVETTTPRAMTQSDIDAV
VGQFRQAARNALNAGFDGIELHAANGYLINQFLDSESNTRTDNYGGSLENRLRFLKEVTQAVSAEIGADRVGVRLAPLTT
LNGTVDANPQETYLAAARLLGELDVTYLHIAEADWDDAPLMAENFKQGLRDAFPNTLIYAGKYDGERARAALKAGWADMI
GFGRPFVANPDLPNRLRHGYPLAPHDPATLFGGGEKGLTDYPTYQALAETQSA
>Mature_372_residues
SHDNLFCPLRLGQLHLNNRIVMPPMTRSRASQPGDVANAIMATYYAQRAEAGLIISEGTQIDPLGKGYAWTPGIHSAEQV
AGWKGVTDAVHAKGGTIFAQLWHVGRVTHTDNIGGAQSISSSAIRAEGVRVFVDNGSDAPGFVETTTPRAMTQSDIDAVV
GQFRQAARNALNAGFDGIELHAANGYLINQFLDSESNTRTDNYGGSLENRLRFLKEVTQAVSAEIGADRVGVRLAPLTTL
NGTVDANPQETYLAAARLLGELDVTYLHIAEADWDDAPLMAENFKQGLRDAFPNTLIYAGKYDGERARAALKAGWADMIG
FGRPFVANPDLPNRLRHGYPLAPHDPATLFGGGEKGLTDYPTYQALAETQSA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=366, Percent_Identity=45.0819672131148, Blast_Score=311, Evalue=5e-86,
Organism=Escherichia coli, GI1789463, Length=352, Percent_Identity=28.9772727272727, Blast_Score=127, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI72001454, Length=314, Percent_Identity=28.0254777070064, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17559802, Length=238, Percent_Identity=30.2521008403361, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17565138, Length=314, Percent_Identity=28.343949044586, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17559804, Length=231, Percent_Identity=32.4675324675325, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17564188, Length=239, Percent_Identity=28.4518828451883, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17540738, Length=310, Percent_Identity=27.4193548387097, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17566914, Length=316, Percent_Identity=25.6329113924051, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=382, Percent_Identity=36.9109947643979, Blast_Score=229, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6321973, Length=385, Percent_Identity=35.5844155844156, Blast_Score=217, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40135; Mature: 40004

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHDNLFCPLRLGQLHLNNRIVMPPMTRSRASQPGDVANAIMATYYAQRAEAGLIISEGT
CCCCCEEEEEEECEEEECCEEECCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEECCC
QIDPLGKGYAWTPGIHSAEQVAGWKGVTDAVHAKGGTIFAQLWHVGRVTHTDNIGGAQSI
EECCCCCCCEECCCCCCHHHHCCCCCCCHHHHCCCCHHHHHHHHHCCEEECCCCCCHHHH
SSSAIRAEGVRVFVDNGSDAPGFVETTTPRAMTQSDIDAVVGQFRQAARNALNAGFDGIE
HHHHHEECCEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
LHAANGYLINQFLDSESNTRTDNYGGSLENRLRFLKEVTQAVSAEIGADRVGVRLAPLTT
EEECCCEEEHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECEEE
LNGTVDANPQETYLAAARLLGELDVTYLHIAEADWDDAPLMAENFKQGLRDAFPNTLIYA
ECCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEE
GKYDGERARAALKAGWADMIGFGRPFVANPDLPNRLRHGYPLAPHDPATLFGGGEKGLTD
CCCCCHHHHHHHHHCHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCEEECCCCCCCCC
YPTYQALAETQSA
CCHHHHHHHHCCC
>Mature Secondary Structure 
SHDNLFCPLRLGQLHLNNRIVMPPMTRSRASQPGDVANAIMATYYAQRAEAGLIISEGT
CCCCEEEEEEECEEEECCEEECCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEECCC
QIDPLGKGYAWTPGIHSAEQVAGWKGVTDAVHAKGGTIFAQLWHVGRVTHTDNIGGAQSI
EECCCCCCCEECCCCCCHHHHCCCCCCCHHHHCCCCHHHHHHHHHCCEEECCCCCCHHHH
SSSAIRAEGVRVFVDNGSDAPGFVETTTPRAMTQSDIDAVVGQFRQAARNALNAGFDGIE
HHHHHEECCEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
LHAANGYLINQFLDSESNTRTDNYGGSLENRLRFLKEVTQAVSAEIGADRVGVRLAPLTT
EEECCCEEEHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECEEE
LNGTVDANPQETYLAAARLLGELDVTYLHIAEADWDDAPLMAENFKQGLRDAFPNTLIYA
ECCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEE
GKYDGERARAALKAGWADMIGFGRPFVANPDLPNRLRHGYPLAPHDPATLFGGGEKGLTD
CCCCCHHHHHHHHHCHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCEEECCCCCCCCC
YPTYQALAETQSA
CCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]