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The map label for this gene is rnt [H]

Identifier: 145299434

GI number: 145299434

Start: 2658347

End: 2659018

Strand: Direct

Name: rnt [H]

Synonym: ASA_2495

Alternate gene names: 145299434

Gene position: 2658347-2659018 (Clockwise)

Preceding gene: 145299431

Following gene: 145299435

Centisome position: 56.53

GC content: 59.97

Gene sequence:

>672_bases
ATGACCGACGCTGTACACACTCTCAAGGGCCGTTTTCGTGGCTTTTACCCCGTCGTCATCGATGTTGAAACCGCCGGGTT
CAATGCCAGGACAGACGCCTTGCTGGAGATAGCCGCCTATACCCTCAAGATGGATGAGGATGGCTGGCTCGCTCCCGATC
AGGTCTTTCATTTTCACGTCGAGCCGTTCGAAGGCGCCAACCTGGAAAAGGCGGCGCTGGAGTTCACCGGCATAGACCCC
TTCAATCCGCTGCGCGGCGCCGTCAGCGAGAAAGAGGCGCTGACCGAGATTTTCAAGGGCATTCGCAAAGGGATGAAGGA
GAGCGACTGCAACCGCGCCATCATAGTGGCCCACAACGCCACCTTCGATCATGGCTTCGTCATGGCTGCTGCCGAGCGGG
CCGGCCTCAAGCGCAATCCGTTCCATCCGTTTGCCACCTTCGATACTGCCGCCCTCTCCGGCCTGGCCCTGGGTCAGACA
GTGCTAGCCAAGGCGTGCCAGAGCGCTCGCATCCCGTTTGACAATAAAGAGGCGCACTCGGCCCTCTATGACACAGAACG
CACCACAGAGCTGTTCTGTCATATTGTCAACCGCTGGAAGAGCCTGGGTGGCTGGCCGCCGGCCCACCCGGATGATGCCC
CCGTCGATGACGCGAACGAACAAGCGGGATAA

Upstream 100 bases:

>100_bases
CCGAGAATAGAATCTGACGTCCGTCTCATGGCTGCTGATGAGGGACAAAGGCTGGCTTTTTTGCCATAATGCCGCCTCAT
TTCACGCCGACCGAGATACC

Downstream 100 bases:

>100_bases
GCAACGAGCAAGAAGGCTGCCATGGCAGCCTTCTTCTATTGGGATACGGCATCAAAGTGGTTTGACCAGAATGGCAGACA
GACCCGGACAGCACAGTTTT

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MTDAVHTLKGRFRGFYPVVIDVETAGFNARTDALLEIAAYTLKMDEDGWLAPDQVFHFHVEPFEGANLEKAALEFTGIDP
FNPLRGAVSEKEALTEIFKGIRKGMKESDCNRAIIVAHNATFDHGFVMAAAERAGLKRNPFHPFATFDTAALSGLALGQT
VLAKACQSARIPFDNKEAHSALYDTERTTELFCHIVNRWKSLGGWPPAHPDDAPVDDANEQAG

Sequences:

>Translated_223_residues
MTDAVHTLKGRFRGFYPVVIDVETAGFNARTDALLEIAAYTLKMDEDGWLAPDQVFHFHVEPFEGANLEKAALEFTGIDP
FNPLRGAVSEKEALTEIFKGIRKGMKESDCNRAIIVAHNATFDHGFVMAAAERAGLKRNPFHPFATFDTAALSGLALGQT
VLAKACQSARIPFDNKEAHSALYDTERTTELFCHIVNRWKSLGGWPPAHPDDAPVDDANEQAG
>Mature_222_residues
TDAVHTLKGRFRGFYPVVIDVETAGFNARTDALLEIAAYTLKMDEDGWLAPDQVFHFHVEPFEGANLEKAALEFTGIDPF
NPLRGAVSEKEALTEIFKGIRKGMKESDCNRAIIVAHNATFDHGFVMAAAERAGLKRNPFHPFATFDTAALSGLALGQTV
LAKACQSARIPFDNKEAHSALYDTERTTELFCHIVNRWKSLGGWPPAHPDDAPVDDANEQAG

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=199, Percent_Identity=78.391959798995, Blast_Score=323, Evalue=5e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 24487; Mature: 24356

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAVHTLKGRFRGFYPVVIDVETAGFNARTDALLEIAAYTLKMDEDGWLAPDQVFHFHV
CCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHEEECCCCCCCCCCEEEEEC
EPFEGANLEKAALEFTGIDPFNPLRGAVSEKEALTEIFKGIRKGMKESDCNRAIIVAHNA
CCCCCCCCHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
TFDHGFVMAAAERAGLKRNPFHPFATFDTAALSGLALGQTVLAKACQSARIPFDNKEAHS
CCCCCCEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
ALYDTERTTELFCHIVNRWKSLGGWPPAHPDDAPVDDANEQAG
HHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDAVHTLKGRFRGFYPVVIDVETAGFNARTDALLEIAAYTLKMDEDGWLAPDQVFHFHV
CCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHEEECCCCCCCCCCEEEEEC
EPFEGANLEKAALEFTGIDPFNPLRGAVSEKEALTEIFKGIRKGMKESDCNRAIIVAHNA
CCCCCCCCHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
TFDHGFVMAAAERAGLKRNPFHPFATFDTAALSGLALGQTVLAKACQSARIPFDNKEAHS
CCCCCCEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
ALYDTERTTELFCHIVNRWKSLGGWPPAHPDDAPVDDANEQAG
HHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]