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The map label for this gene is hisJ [H]

Identifier: 145299355

GI number: 145299355

Start: 2564562

End: 2565338

Strand: Direct

Name: hisJ [H]

Synonym: ASA_2403

Alternate gene names: 145299355

Gene position: 2564562-2565338 (Clockwise)

Preceding gene: 145299354

Following gene: 145299356

Centisome position: 54.54

GC content: 61.65

Gene sequence:

>777_bases
ATGACCATGAAACTCAATACCGCAATGATGCTGGTGGCCCTGGCCACCGCCGGCCAGGCCGCCGCCAAACCCCTGGTGAT
CGCCACCGACGCCACCTATCCCCCCTTCGAGATGGTCGATAGCAGCGGCAAGCTCGGCGGCTTCGAAGTGGATCTGGCCT
ATGCCCTGTGCGCGGCCATGAAGACGGAGTGCGAGGTGATCAACCAGCCCTGGGATGCCCTGCTCCCCGGCCTGCAGGTG
CGCAAGTACGACGCCATCATGTCCTCCATGAACATCACGGACGAGCGGCGTCAGAAGGTCGCCTTCAGCCAGGTCTACTA
CATGATGCAGAACCGCTTCGTGGGACGCAGCGACAAGGCGGCCGACTTTGCCGACACCCCTGAGTACTTCAAGGGCAAGG
TGATAGCGGTGCAGGAAGGGACACCCCAGGACAACTTCATCACCGCCCACTACGGTGACGTGGCCAAGATCAAGCGCTAC
GTCAACGCCCAGTCCCCCATGCTGGATCTGCAGTCCAGCCGCGCCGACTACACCTTCGGCAACATGGTGCAGTTGAAGGT
CGGCTTCCTCGACAAGGAGATGGGCAAGCAGTTCGCCTTCGTCGGTCCCCAGTTCAACGGCACCCAGGACAAGATCCTGG
GAGAAGGGGTGGCGGTGGCCCTGCGCAAAAACGATAACAAGCTCAAGGCCCGCTTCGATGCCGCCATCGAGAGCGTCAAG
GCCGACGGCACCTTCGACACCCTGCTCAAGAAGTACCAGCTCGAAGACCTGCTCTGA

Upstream 100 bases:

>100_bases
GCTGCCGGCCCTCAGCCATCGCAAGATATCCGTCGGCCCCGCAAGCCGACACAACTCATGACTATCGGCATGACGCCACA
GACGCCACAACTGCAAGGGA

Downstream 100 bases:

>100_bases
TCCCGACGCCGGGCATCCGTCAGGCCATACCTTGCATGCCAGTCGACACCGACTGGCATTTTTTTACCCGGGTTCTGCCG
GCAACGCGGCAAACTGCCCT

Product: ABC-type amino acid transporter, periplasmic binding protein

Products: ADP; phosphate; L-histidine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MTMKLNTAMMLVALATAGQAAAKPLVIATDATYPPFEMVDSSGKLGGFEVDLAYALCAAMKTECEVINQPWDALLPGLQV
RKYDAIMSSMNITDERRQKVAFSQVYYMMQNRFVGRSDKAADFADTPEYFKGKVIAVQEGTPQDNFITAHYGDVAKIKRY
VNAQSPMLDLQSSRADYTFGNMVQLKVGFLDKEMGKQFAFVGPQFNGTQDKILGEGVAVALRKNDNKLKARFDAAIESVK
ADGTFDTLLKKYQLEDLL

Sequences:

>Translated_258_residues
MTMKLNTAMMLVALATAGQAAAKPLVIATDATYPPFEMVDSSGKLGGFEVDLAYALCAAMKTECEVINQPWDALLPGLQV
RKYDAIMSSMNITDERRQKVAFSQVYYMMQNRFVGRSDKAADFADTPEYFKGKVIAVQEGTPQDNFITAHYGDVAKIKRY
VNAQSPMLDLQSSRADYTFGNMVQLKVGFLDKEMGKQFAFVGPQFNGTQDKILGEGVAVALRKNDNKLKARFDAAIESVK
ADGTFDTLLKKYQLEDLL
>Mature_257_residues
TMKLNTAMMLVALATAGQAAAKPLVIATDATYPPFEMVDSSGKLGGFEVDLAYALCAAMKTECEVINQPWDALLPGLQVR
KYDAIMSSMNITDERRQKVAFSQVYYMMQNRFVGRSDKAADFADTPEYFKGKVIAVQEGTPQDNFITAHYGDVAKIKRYV
NAQSPMLDLQSSRADYTFGNMVQLKVGFLDKEMGKQFAFVGPQFNGTQDKILGEGVAVALRKNDNKLKARFDAAIESVKA
DGTFDTLLKKYQLEDLL

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788648, Length=229, Percent_Identity=38.8646288209607, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI1788649, Length=247, Percent_Identity=35.6275303643725, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1787088, Length=242, Percent_Identity=34.297520661157, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1787085, Length=247, Percent_Identity=32.3886639676113, Blast_Score=143, Evalue=9e-36,
Organism=Escherichia coli, GI1787031, Length=250, Percent_Identity=25.6, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788228, Length=228, Percent_Identity=26.7543859649123, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: 3400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28553; Mature: 28422

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMKLNTAMMLVALATAGQAAAKPLVIATDATYPPFEMVDSSGKLGGFEVDLAYALCAAM
CEEEEHHHHHHHHHHHCCHHHCCCEEEEECCCCCCHHHHCCCCCCCCEEHHHHHHHHHHH
KTECEVINQPWDALLPGLQVRKYDAIMSSMNITDERRQKVAFSQVYYMMQNRFVGRSDKA
HHHHHHHCCCHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
ADFADTPEYFKGKVIAVQEGTPQDNFITAHYGDVAKIKRYVNAQSPMLDLQSSRADYTFG
CCCCCCHHHHCCEEEEEECCCCCCCEEEEECCCHHHHHHHHCCCCCCEEHHCCCCCCCCC
NMVQLKVGFLDKEMGKQFAFVGPQFNGTQDKILGEGVAVALRKNDNKLKARFDAAIESVK
CEEEEEECCCCHHHCCEEEEECCCCCCCHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHC
ADGTFDTLLKKYQLEDLL
CCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TMKLNTAMMLVALATAGQAAAKPLVIATDATYPPFEMVDSSGKLGGFEVDLAYALCAAM
EEEEHHHHHHHHHHHCCHHHCCCEEEEECCCCCCHHHHCCCCCCCCEEHHHHHHHHHHH
KTECEVINQPWDALLPGLQVRKYDAIMSSMNITDERRQKVAFSQVYYMMQNRFVGRSDKA
HHHHHHHCCCHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
ADFADTPEYFKGKVIAVQEGTPQDNFITAHYGDVAKIKRYVNAQSPMLDLQSSRADYTFG
CCCCCCHHHHCCEEEEEECCCCCCCEEEEECCCHHHHHHHHCCCCCCEEHHCCCCCCCCC
NMVQLKVGFLDKEMGKQFAFVGPQFNGTQDKILGEGVAVALRKNDNKLKARFDAAIESVK
CEEEEEECCCCHHHCCEEEEECCCCCCCHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHC
ADGTFDTLLKKYQLEDLL
CCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]