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The map label for this gene is yafV [C]

Identifier: 145299354

GI number: 145299354

Start: 2563668

End: 2564522

Strand: Direct

Name: yafV [C]

Synonym: ASA_2402

Alternate gene names: 145299354

Gene position: 2563668-2564522 (Clockwise)

Preceding gene: 145299352

Following gene: 145299355

Centisome position: 54.52

GC content: 67.6

Gene sequence:

>855_bases
GTGCGGCGTCTAGGAGGCAACATGGATTCAGTGCGCGTTGCCGTCCTGCAGATGGTCTCCGGGGATGACCTTGACCACAA
CCTGACCCAGGCCGAGGCCCTGCTGCGTCAGGCCGCGGCCGAGGGGGCCGAGTTTGCCCTGCTGCCCGAATACTTCTACC
TGATGCCTGCCGACGAGCGGGCACGGGTGGCCCTGGCCGCCCCCGTGAGCGATCACCCTCTGCTGGCATGGGCGCAAGGG
CTGGCCCGGGAGCTCGGGATCTGGCTGCTGGCGGGCACCCTGCCCCTTGAAAGTGATGAGCCCGGCAAGATGCACAACAG
CAGCCTGCTCATCGACCCGCAAGGGGCCCTGGCCAGCCGCTACGACAAGCTGCACCTGTTCGGCTTTTGCACCGGGCAAG
AGCAGTATGACGAGGCCGCAACCATGAGCCCGGGCCGGGAGGTGGTGAGCCATCCCCTGCCCTGGGGGATGCTGCGCTTC
GGGATCTGCTACGACCTGCGCTTCCCCGAGCTGTTTCGACTCGATCCTGCGCCCGATTTCATCGCCCTGCCCGCCGCCTT
CACCCACACCACCGGGCTGGCCCATTGGGAGCTGCTGCTGCGGGCCAGAGCGGTGGAGAACCTGGCCTTCGTGCTGGCCT
CCGCCCAGGGGGGCCATCACCCGGGTGGACGCCGCACCTTCGGCCACAGCATGATCATCGATCCCTGGGGCCAAGTGTTG
GCCCGGGTGGAAGAGGGTCATGGCATGGCCATCGCCACCCTGGATCTGGCCGCCCAGCGCAAGGTACGCAGCCAGCTGCC
GGCCCTCAGCCATCGCAAGATATCCGTCGGCCCCGCAAGCCGACACAACTCATGA

Upstream 100 bases:

>100_bases
TTCCGGTCTCCGCCTTCTATCACGACAGCACGGATCAGCGGGTCATCCGCCTCTCCTTCTCCAAGGATGACGATACCCTG
CTGGCGGGCGCCGAGCGCCT

Downstream 100 bases:

>100_bases
CTATCGGCATGACGCCACAGACGCCACAACTGCAAGGGAATGACCATGAAACTCAATACCGCAATGATGCTGGTGGCCCT
GGCCACCGCCGGCCAGGCCG

Product: amidohydrolase family protein

Products: carboxylate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MRRLGGNMDSVRVAVLQMVSGDDLDHNLTQAEALLRQAAAEGAEFALLPEYFYLMPADERARVALAAPVSDHPLLAWAQG
LARELGIWLLAGTLPLESDEPGKMHNSSLLIDPQGALASRYDKLHLFGFCTGQEQYDEAATMSPGREVVSHPLPWGMLRF
GICYDLRFPELFRLDPAPDFIALPAAFTHTTGLAHWELLLRARAVENLAFVLASAQGGHHPGGRRTFGHSMIIDPWGQVL
ARVEEGHGMAIATLDLAAQRKVRSQLPALSHRKISVGPASRHNS

Sequences:

>Translated_284_residues
MRRLGGNMDSVRVAVLQMVSGDDLDHNLTQAEALLRQAAAEGAEFALLPEYFYLMPADERARVALAAPVSDHPLLAWAQG
LARELGIWLLAGTLPLESDEPGKMHNSSLLIDPQGALASRYDKLHLFGFCTGQEQYDEAATMSPGREVVSHPLPWGMLRF
GICYDLRFPELFRLDPAPDFIALPAAFTHTTGLAHWELLLRARAVENLAFVLASAQGGHHPGGRRTFGHSMIIDPWGQVL
ARVEEGHGMAIATLDLAAQRKVRSQLPALSHRKISVGPASRHNS
>Mature_284_residues
MRRLGGNMDSVRVAVLQMVSGDDLDHNLTQAEALLRQAAAEGAEFALLPEYFYLMPADERARVALAAPVSDHPLLAWAQG
LARELGIWLLAGTLPLESDEPGKMHNSSLLIDPQGALASRYDKLHLFGFCTGQEQYDEAATMSPGREVVSHPLPWGMLRF
GICYDLRFPELFRLDPAPDFIALPAAFTHTTGLAHWELLLRARAVENLAFVLASAQGGHHPGGRRTFGHSMIIDPWGQVL
ARVEEGHGMAIATLDLAAQRKVRSQLPALSHRKISVGPASRHNS

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632348, Length=268, Percent_Identity=38.0597014925373, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI297632350, Length=269, Percent_Identity=38.2899628252788, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI5031947, Length=268, Percent_Identity=38.0597014925373, Blast_Score=174, Evalue=7e-44,
Organism=Homo sapiens, GI9910460, Length=269, Percent_Identity=31.5985130111524, Blast_Score=157, Evalue=9e-39,
Organism=Homo sapiens, GI297632346, Length=192, Percent_Identity=33.8541666666667, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1786412, Length=228, Percent_Identity=31.140350877193, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17556280, Length=270, Percent_Identity=37.7777777777778, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6323383, Length=275, Percent_Identity=29.8181818181818, Blast_Score=132, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6322335, Length=299, Percent_Identity=31.438127090301, Blast_Score=123, Evalue=3e-29,
Organism=Drosophila melanogaster, GI17933642, Length=279, Percent_Identity=34.7670250896057, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI21355835, Length=275, Percent_Identity=29.8181818181818, Blast_Score=137, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.5.1

Molecular weight: Translated: 31060; Mature: 31060

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLGGNMDSVRVAVLQMVSGDDLDHNLTQAEALLRQAAAEGAEFALLPEYFYLMPADER
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCEEEEECCCCC
ARVALAAPVSDHPLLAWAQGLARELGIWLLAGTLPLESDEPGKMHNSSLLIDPQGALASR
CEEEEECCCCCCCHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHHH
YDKLHLFGFCTGQEQYDEAATMSPGREVVSHPLPWGMLRFGICYDLRFPELFRLDPAPDF
CCEEEEEEEECCCHHHHHHHCCCCCHHHHCCCCCHHHHHHHHEEECCCCHHEECCCCCCE
IALPAAFTHTTGLAHWELLLRARAVENLAFVLASAQGGHHPGGRRTFGHSMIIDPWGQVL
EECCCHHHHHCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEECCHHHHH
ARVEEGHGMAIATLDLAAQRKVRSQLPALSHRKISVGPASRHNS
HHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure
MRRLGGNMDSVRVAVLQMVSGDDLDHNLTQAEALLRQAAAEGAEFALLPEYFYLMPADER
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCEEEEECCCCC
ARVALAAPVSDHPLLAWAQGLARELGIWLLAGTLPLESDEPGKMHNSSLLIDPQGALASR
CEEEEECCCCCCCHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHHH
YDKLHLFGFCTGQEQYDEAATMSPGREVVSHPLPWGMLRFGICYDLRFPELFRLDPAPDF
CCEEEEEEEECCCHHHHHHHCCCCCHHHHCCCCCHHHHHHHHEEECCCCHHEECCCCCCE
IALPAAFTHTTGLAHWELLLRARAVENLAFVLASAQGGHHPGGRRTFGHSMIIDPWGQVL
EECCCHHHHHCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEECCHHHHH
ARVEEGHGMAIATLDLAAQRKVRSQLPALSHRKISVGPASRHNS
HHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrile; H2O

Specific reaction: A nitrile + H2O = a carboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]