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The map label for this gene is mdeA [H]

Identifier: 145299303

GI number: 145299303

Start: 2493091

End: 2494254

Strand: Direct

Name: mdeA [H]

Synonym: ASA_2347

Alternate gene names: 145299303

Gene position: 2493091-2494254 (Clockwise)

Preceding gene: 145299302

Following gene: 145299306

Centisome position: 53.02

GC content: 63.83

Gene sequence:

>1164_bases
ATGAAATTCAAGACAATGAGCGTGCACAGCGGCCAACGGATCGATCCCCAGACCGGCGCCCTCACCACCCCCCTCTACCA
GAGCAGCACCTTCAGCTACTTCAATGCCCAGGCGGGCAAGGAGCGTTTCGCGGGTCAGGCCCCCGGCTTCATCTACAGCC
GCTTTGGCAATCCCACCACAGCCGAGCTGGAGCAAAAACTGGCGGCCCTTGAGGGCGCCGACGAGGCGTTGGTGGTGGCA
AGCGGCATGGCGGCGGTGAGCGCCATCATGTATGCCCTGGCCGACAGCGGCGATGAGGTGGCCTACATAGGTCCGCTCTA
CGGCGGCACAGATGCCTTCCTGAAACAGACCTTCAGCCGTGCCGGCATCAAGGTCACCGCCTATGAGAGCGATCAGGATC
TGCTGGCCAACATTCGTCCCGCCACCAAGGTGGTACTGTTCGAAACCCTGACCAACCCCACCCTCAAGGTGGTGGATCCG
CGGGTGGTAGTGGAAGCGGCCCGCAAGGTGGGTGCCATCAGCGTGTGTGACAACACCTTCCTCACCCCCTATCTGCTGCG
CCCACTCGAGCTTGGCATCGACATAGTGATGCACAGCGGCACCAAATATCTAGGCGGCCACGGCGACATCATCGCCGGGG
TAGTGGCCGGCTCCCACGCGCTGATGAAATCCATCCGCACCGTGGCGCTCAAGCACATTGGATCCCCCATAGGCCCGCAG
GAGGCTTACCTGCTGCAGCGCGGAGTCAAGACCCTACCCCTGCGCATGGATGCCCACCTGCACAACGCTCAGAAAGTGGC
CGACTTCCTGGCCGCTCACCCGGCGGTGAAGAAGGTGATCTACCCGGGCCTCGTCAGCCACCCGGGCCACGATGCCCTCG
GCGCCTTCGCCAGCGGCTTTGGCGGCATGATCAGTATCGAGCTGGAAGGGGGCTTTGCGCGCTGCGCCACCCTGCTCGAC
AACCTGCAACTGTTCGTGCAGGCGGTGAGCTTCGGCGATCTCGAGAGCCTGGCCTGCCATCCGGCCAGCACCACCCACGC
CGCCATGGATGAAGCGAGCCGTCTCGCCGCCGGGGTGAATGACGATCTCATCCGCTTCAGCATTGGCGTGGAGGACGCCG
ACGATCTGATCGCCGATCTGGCAGCGGCACTGGCCCTCGTCTGA

Upstream 100 bases:

>100_bases
CTGACTCTGCCATACCTATTAAACGGGGGATGCCGTGGCGTCCCGCCACCCGGATGACACAAGCAGTTAAGCCACAGCCT
GGCAATGACGGAGTAATAGA

Downstream 100 bases:

>100_bases
CGGACAAAGGCTAACCTTTACGGCTGACAGACAATCTGACGTACAAAGAGCCCGCCCCATCGCTGTGATGGGGCGGGCTC
TTTTATGGCTACTTGACTGC

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MKFKTMSVHSGQRIDPQTGALTTPLYQSSTFSYFNAQAGKERFAGQAPGFIYSRFGNPTTAELEQKLAALEGADEALVVA
SGMAAVSAIMYALADSGDEVAYIGPLYGGTDAFLKQTFSRAGIKVTAYESDQDLLANIRPATKVVLFETLTNPTLKVVDP
RVVVEAARKVGAISVCDNTFLTPYLLRPLELGIDIVMHSGTKYLGGHGDIIAGVVAGSHALMKSIRTVALKHIGSPIGPQ
EAYLLQRGVKTLPLRMDAHLHNAQKVADFLAAHPAVKKVIYPGLVSHPGHDALGAFASGFGGMISIELEGGFARCATLLD
NLQLFVQAVSFGDLESLACHPASTTHAAMDEASRLAAGVNDDLIRFSIGVEDADDLIADLAAALALV

Sequences:

>Translated_387_residues
MKFKTMSVHSGQRIDPQTGALTTPLYQSSTFSYFNAQAGKERFAGQAPGFIYSRFGNPTTAELEQKLAALEGADEALVVA
SGMAAVSAIMYALADSGDEVAYIGPLYGGTDAFLKQTFSRAGIKVTAYESDQDLLANIRPATKVVLFETLTNPTLKVVDP
RVVVEAARKVGAISVCDNTFLTPYLLRPLELGIDIVMHSGTKYLGGHGDIIAGVVAGSHALMKSIRTVALKHIGSPIGPQ
EAYLLQRGVKTLPLRMDAHLHNAQKVADFLAAHPAVKKVIYPGLVSHPGHDALGAFASGFGGMISIELEGGFARCATLLD
NLQLFVQAVSFGDLESLACHPASTTHAAMDEASRLAAGVNDDLIRFSIGVEDADDLIADLAAALALV
>Mature_387_residues
MKFKTMSVHSGQRIDPQTGALTTPLYQSSTFSYFNAQAGKERFAGQAPGFIYSRFGNPTTAELEQKLAALEGADEALVVA
SGMAAVSAIMYALADSGDEVAYIGPLYGGTDAFLKQTFSRAGIKVTAYESDQDLLANIRPATKVVLFETLTNPTLKVVDP
RVVVEAARKVGAISVCDNTFLTPYLLRPLELGIDIVMHSGTKYLGGHGDIIAGVVAGSHALMKSIRTVALKHIGSPIGPQ
EAYLLQRGVKTLPLRMDAHLHNAQKVADFLAAHPAVKKVIYPGLVSHPGHDALGAFASGFGGMISIELEGGFARCATLLD
NLQLFVQAVSFGDLESLACHPASTTHAAMDEASRLAAGVNDDLIRFSIGVEDADDLIADLAAALALV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=374, Percent_Identity=40.9090909090909, Blast_Score=258, Evalue=1e-68,
Organism=Homo sapiens, GI299473759, Length=374, Percent_Identity=37.4331550802139, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI25453487, Length=371, Percent_Identity=35.5795148247978, Blast_Score=207, Evalue=1e-53,
Organism=Escherichia coli, GI1790375, Length=383, Percent_Identity=38.3812010443864, Blast_Score=242, Evalue=2e-65,
Organism=Escherichia coli, GI1789383, Length=393, Percent_Identity=30.7888040712468, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71980708, Length=395, Percent_Identity=37.9746835443038, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17560056, Length=390, Percent_Identity=40, Blast_Score=243, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI17538055, Length=393, Percent_Identity=39.1857506361323, Blast_Score=238, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6319307, Length=398, Percent_Identity=38.4422110552764, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=34.4036697247706, Blast_Score=210, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6321254, Length=415, Percent_Identity=31.566265060241, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321170, Length=329, Percent_Identity=28.5714285714286, Blast_Score=139, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6321904, Length=396, Percent_Identity=29.040404040404, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19922554, Length=390, Percent_Identity=39.2307692307692, Blast_Score=254, Evalue=7e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 40855; Mature: 40855

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKTMSVHSGQRIDPQTGALTTPLYQSSTFSYFNAQAGKERFAGQAPGFIYSRFGNPTT
CCEEEEECCCCCCCCCCCCCEECCCCCCCCCHHHHHHCCHHHHCCCCCCCEEECCCCCCH
AELEQKLAALEGADEALVVASGMAAVSAIMYALADSGDEVAYIGPLYGGTDAFLKQTFSR
HHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
AGIKVTAYESDQDLLANIRPATKVVLFETLTNPTLKVVDPRVVVEAARKVGAISVCDNTF
CCEEEEEECCCHHHHHCCCCHHEEEEEECCCCCEEEEECHHHHHHHHHHCCEEEECCCCC
LTPYLLRPLELGIDIVMHSGTKYLGGHGDIIAGVVAGSHALMKSIRTVALKHIGSPIGPQ
CHHHHHHHHHHCEEEEEECCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHCCCCCCCH
EAYLLQRGVKTLPLRMDAHLHNAQKVADFLAAHPAVKKVIYPGLVSHPGHDALGAFASGF
HHHHHHCCCHHCCEEECHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCHHHHHHHCCC
GGMISIELEGGFARCATLLDNLQLFVQAVSFGDLESLACHPASTTHAAMDEASRLAAGVN
CCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCC
DDLIRFSIGVEDADDLIADLAAALALV
CCEEEEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFKTMSVHSGQRIDPQTGALTTPLYQSSTFSYFNAQAGKERFAGQAPGFIYSRFGNPTT
CCEEEEECCCCCCCCCCCCCEECCCCCCCCCHHHHHHCCHHHHCCCCCCCEEECCCCCCH
AELEQKLAALEGADEALVVASGMAAVSAIMYALADSGDEVAYIGPLYGGTDAFLKQTFSR
HHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
AGIKVTAYESDQDLLANIRPATKVVLFETLTNPTLKVVDPRVVVEAARKVGAISVCDNTF
CCEEEEEECCCHHHHHCCCCHHEEEEEECCCCCEEEEECHHHHHHHHHHCCEEEECCCCC
LTPYLLRPLELGIDIVMHSGTKYLGGHGDIIAGVVAGSHALMKSIRTVALKHIGSPIGPQ
CHHHHHHHHHHCEEEEEECCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHCCCCCCCH
EAYLLQRGVKTLPLRMDAHLHNAQKVADFLAAHPAVKKVIYPGLVSHPGHDALGAFASGF
HHHHHHCCCHHCCEEECHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCHHHHHHHCCC
GGMISIELEGGFARCATLLDNLQLFVQAVSFGDLESLACHPASTTHAAMDEASRLAAGVN
CCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCC
DDLIRFSIGVEDADDLIADLAAALALV
CCEEEEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]