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The map label for this gene is lutR [H]

Identifier: 145296907

GI number: 145296907

Start: 3117211

End: 3117927

Strand: Direct

Name: lutR [H]

Synonym: cgR_2807

Alternate gene names: 145296907

Gene position: 3117211-3117927 (Clockwise)

Preceding gene: 145296906

Following gene: 145296908

Centisome position: 94.06

GC content: 52.86

Gene sequence:

>717_bases
ATGATGTTCACCATGAACCTGTCTGACAGCTGGACACCCCGACAGCCGGTGCTCTCTCGCACTAGCGCTGCGGAGGAAGT
CTTCAATGCAATTCGTCAAGGAATCGAATCTGGGGAAATCCCCCTTGGTTCCAAACTCAGCTCCGAAGCGGCGTTAGCTG
CATCCTTTGGGGTAAGTCGCTCGGTGATTCGAGAAGCACTTCGATCTTCAGCAACTTTGGGACTAACCAAGACTGAAACG
GGTCGCGGCACTTTTGTTATTTCGGCATCCCCCACCAACGATCTTGTGTTGGGTAATTTTTCTTCCACCGATCTTTATGA
GGCCCGACCTCATATTGAAGTTCCTGCTGCTGGCCTCGCTGCAACCAGGCGATCGAAGGATGATTTGCAGCAGCTTTTTA
ACATCATCGAGCAGATGGAAGCCGAAGATGATCACGTAGCTTGGGTGGAATTGGACACGGAATTTCACGCTGCCATCGCT
AACGCCAGTGGAAATTCGGTGTTCGCCAAGATCGTCGGCGATATTCGTGAATCCTTGGCCAGGCAGTCTGAGACCGTCAA
CGTGGTGTCTGGTCGCAGGGAGCCATCCGATGCAGAACACCGCGCGATCGTGGAAGCAATCGCCGCGCAAGATAAGGATG
CTGCTGAAGCTGCCATGGCATTCCACCTTTCCGCTGTTCAGGTGGCTGTTGATGACATCGTGAGCAAACAATCATGA

Upstream 100 bases:

>100_bases
GAGCCTGTTCCCTTTTCGGGGCAGGTTCTTTTTATTGCCATAACCTGCACAAAGAGAATCACTTAACAAGAAGTCCTTGA
CGTAAGGTGCATCACAATGC

Downstream 100 bases:

>100_bases
TCCCAGCGCACGCCCCACTCGTTGGCCAAACCCGTGGCGATCACATTGAATCCATCCACTACGGATCAGCTGTGCTGCTT
GATGTTGATGGAAATATCGA

Product: hypothetical protein

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MMFTMNLSDSWTPRQPVLSRTSAAEEVFNAIRQGIESGEIPLGSKLSSEAALAASFGVSRSVIREALRSSATLGLTKTET
GRGTFVISASPTNDLVLGNFSSTDLYEARPHIEVPAAGLAATRRSKDDLQQLFNIIEQMEAEDDHVAWVELDTEFHAAIA
NASGNSVFAKIVGDIRESLARQSETVNVVSGRREPSDAEHRAIVEAIAAQDKDAAEAAMAFHLSAVQVAVDDIVSKQS

Sequences:

>Translated_238_residues
MMFTMNLSDSWTPRQPVLSRTSAAEEVFNAIRQGIESGEIPLGSKLSSEAALAASFGVSRSVIREALRSSATLGLTKTET
GRGTFVISASPTNDLVLGNFSSTDLYEARPHIEVPAAGLAATRRSKDDLQQLFNIIEQMEAEDDHVAWVELDTEFHAAIA
NASGNSVFAKIVGDIRESLARQSETVNVVSGRREPSDAEHRAIVEAIAAQDKDAAEAAMAFHLSAVQVAVDDIVSKQS
>Mature_238_residues
MMFTMNLSDSWTPRQPVLSRTSAAEEVFNAIRQGIESGEIPLGSKLSSEAALAASFGVSRSVIREALRSSATLGLTKTET
GRGTFVISASPTNDLVLGNFSSTDLYEARPHIEVPAAGLAATRRSKDDLQQLFNIIEQMEAEDDHVAWVELDTEFHAAIA
NASGNSVFAKIVGDIRESLARQSETVNVVSGRREPSDAEHRAIVEAIAAQDKDAAEAAMAFHLSAVQVAVDDIVSKQS

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082208, Length=214, Percent_Identity=29.9065420560748, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1790032, Length=222, Percent_Identity=31.0810810810811, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789621, Length=250, Percent_Identity=27.6, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI48994955, Length=201, Percent_Identity=31.8407960199005, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786303, Length=237, Percent_Identity=29.957805907173, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 25501; Mature: 25501

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMFTMNLSDSWTPRQPVLSRTSAAEEVFNAIRQGIESGEIPLGSKLSSEAALAASFGVSR
CEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCH
SVIREALRSSATLGLTKTETGRGTFVISASPTNDLVLGNFSSTDLYEARPHIEVPAAGLA
HHHHHHHHCCCCCCEEECCCCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCHHH
ATRRSKDDLQQLFNIIEQMEAEDDHVAWVELDTEFHAAIANASGNSVFAKIVGDIRESLA
HHCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHEECCCCCHHHHHHHHHHHHHHH
RQSETVNVVSGRREPSDAEHRAIVEAIAAQDKDAAEAAMAFHLSAVQVAVDDIVSKQS
HHHCCEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMFTMNLSDSWTPRQPVLSRTSAAEEVFNAIRQGIESGEIPLGSKLSSEAALAASFGVSR
CEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCH
SVIREALRSSATLGLTKTETGRGTFVISASPTNDLVLGNFSSTDLYEARPHIEVPAAGLA
HHHHHHHHCCCCCCEEECCCCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCHHH
ATRRSKDDLQQLFNIIEQMEAEDDHVAWVELDTEFHAAIANASGNSVFAKIVGDIRESLA
HHCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHEECCCCCHHHHHHHHHHHHHHH
RQSETVNVVSGRREPSDAEHRAIVEAIAAQDKDAAEAAMAFHLSAVQVAVDDIVSKQS
HHHCCEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]