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The map label for this gene is kgtP [H]

Identifier: 145296900

GI number: 145296900

Start: 3108858

End: 3110186

Strand: Direct

Name: kgtP [H]

Synonym: cgR_2800

Alternate gene names: 145296900

Gene position: 3108858-3110186 (Clockwise)

Preceding gene: 145296897

Following gene: 145296901

Centisome position: 93.8

GC content: 52.67

Gene sequence:

>1329_bases
ATGACTGCAATTACGCCTCAGCCACAAAATGTTGAATCTGGGAGTTCCAAAGGCATACGCGAAGAGAAGAACTTCGTTCC
GGGAATCCCGATGGCCCAGCTTCGCTCGGGCGCAATCGGAAATCTCATCGAGTGGTTCGACTGGAATGCCTACGCATTGC
TTTCTGTATATTTCGCTGCACAGTTCTTCCCAGCAGGCACCTCGCCACTTGTTGCACTCCTTGGTTCCTTCGGAATCATG
GCGGTGGGATTCATCGTTCGTCCACTCGCGGGCCTAGCTATCGGTGTAATCGCCGACCGGTTTGGACGTAAAACCGCAAT
GTTGATCACCGTGTACGGCATGGGCATTTCCAGCCTTATGATTGCCCTTTCTCCGACATACGCACAGATCGGCATTTTCG
CACCGTTCATCTTGTTGATCGCACGCATCATTCAAGGTATTTGCATCGGTGGCGAGTACGCCGCACTGACAGCCTTTGCC
ATGGAAATGTCACCAAACGGCAAGCGAGGCCTTGTTGCCGCGATCCTCAATATTGTGGCGAACTTCGGACAAATTCTCGT
CGTCGTCATCATTTTGGCCTTCTCCTGGTCCCTGCCAGACGATGCTATGCACACCTGGGGCTGGCGCCTCGTATTCATCA
TCGGCGCTCTCCTTTCACTGCTCGGCATTTGGATGCGCCGCGACATGAAGGAAACCATTGACGTAGACACCTCACGCGCA
AAGAAGGAAAGTGTTTTTGCAGCGGTCAAACGTTACCCACGTCAAACCATCCAGATCATTGGCCTAACTGCCGGCTTCAC
CGCAATGGTGTACGCATGGGGTGCCTACATGCCAACCTATGCGGCTACTTACAAGGGATTCGATCCTAAGTACAGCATCA
TGTCCATGGGAGCAAGCTTCGCGGTTGCAATGGTTAGTACGTACTTTGCAGGTAAAGCATCTGACCGTTTTGGACGAAAG
CCCACAATGATCATCGCGGGTATCCTCTTGTCTGTTGGTACTGTCCCTGCGTTGACCTTGCTCAACGATTCAGTCTGGAC
CCTCATCATTATCCAATCTGTCGGTCTCAGCATCATCGGCATGCTCCAATCCAGTTCGATGCCAGTATTCGCTGAAATGT
TCCCGTCCGATTTCCGGGCATCCGGGCTTGGCTTCCCTTACGCCCTCACTGTTGGTCTCATCGGCGGCACCGTCCCTCTC
GTCGGTACCCAGCTTGCAAATATGAACCTCACCTCAGTATTCCCCTGGTACCTCACCGCGCTGATTGTGATTTCCACACT
GTTCTACATCGGCATGAAAGAAACGGCATTCAAGCCACTACCGAAGTAA

Upstream 100 bases:

>100_bases
TTGAGTGTGCGACACTTCTAGTGTTCCACACCACAGAAGTTTCCACCAGAGATTTAAAACACTTTTTGAAATACATTGAA
ATTCCACAATTGGAGGAATC

Downstream 100 bases:

>100_bases
GTCACTTCCCCCAAAAGTCACCACACCTATTTAGAAAGAATCTTCAGTGTTTAACACCTTGTCTTTGGAACAGCTCCGTC
AGCGCGGCACCCTCAAATGG

Product: hypothetical protein

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTAITPQPQNVESGSSKGIREEKNFVPGIPMAQLRSGAIGNLIEWFDWNAYALLSVYFAAQFFPAGTSPLVALLGSFGIM
AVGFIVRPLAGLAIGVIADRFGRKTAMLITVYGMGISSLMIALSPTYAQIGIFAPFILLIARIIQGICIGGEYAALTAFA
MEMSPNGKRGLVAAILNIVANFGQILVVVIILAFSWSLPDDAMHTWGWRLVFIIGALLSLLGIWMRRDMKETIDVDTSRA
KKESVFAAVKRYPRQTIQIIGLTAGFTAMVYAWGAYMPTYAATYKGFDPKYSIMSMGASFAVAMVSTYFAGKASDRFGRK
PTMIIAGILLSVGTVPALTLLNDSVWTLIIIQSVGLSIIGMLQSSSMPVFAEMFPSDFRASGLGFPYALTVGLIGGTVPL
VGTQLANMNLTSVFPWYLTALIVISTLFYIGMKETAFKPLPK

Sequences:

>Translated_442_residues
MTAITPQPQNVESGSSKGIREEKNFVPGIPMAQLRSGAIGNLIEWFDWNAYALLSVYFAAQFFPAGTSPLVALLGSFGIM
AVGFIVRPLAGLAIGVIADRFGRKTAMLITVYGMGISSLMIALSPTYAQIGIFAPFILLIARIIQGICIGGEYAALTAFA
MEMSPNGKRGLVAAILNIVANFGQILVVVIILAFSWSLPDDAMHTWGWRLVFIIGALLSLLGIWMRRDMKETIDVDTSRA
KKESVFAAVKRYPRQTIQIIGLTAGFTAMVYAWGAYMPTYAATYKGFDPKYSIMSMGASFAVAMVSTYFAGKASDRFGRK
PTMIIAGILLSVGTVPALTLLNDSVWTLIIIQSVGLSIIGMLQSSSMPVFAEMFPSDFRASGLGFPYALTVGLIGGTVPL
VGTQLANMNLTSVFPWYLTALIVISTLFYIGMKETAFKPLPK
>Mature_441_residues
TAITPQPQNVESGSSKGIREEKNFVPGIPMAQLRSGAIGNLIEWFDWNAYALLSVYFAAQFFPAGTSPLVALLGSFGIMA
VGFIVRPLAGLAIGVIADRFGRKTAMLITVYGMGISSLMIALSPTYAQIGIFAPFILLIARIIQGICIGGEYAALTAFAM
EMSPNGKRGLVAAILNIVANFGQILVVVIILAFSWSLPDDAMHTWGWRLVFIIGALLSLLGIWMRRDMKETIDVDTSRAK
KESVFAAVKRYPRQTIQIIGLTAGFTAMVYAWGAYMPTYAATYKGFDPKYSIMSMGASFAVAMVSTYFAGKASDRFGRKP
TMIIAGILLSVGTVPALTLLNDSVWTLIIIQSVGLSIIGMLQSSSMPVFAEMFPSDFRASGLGFPYALTVGLIGGTVPLV
GTQLANMNLTSVFPWYLTALIVISTLFYIGMKETAFKPLPK

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=390, Percent_Identity=34.3589743589744, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1790550, Length=423, Percent_Identity=30.0236406619385, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1788292, Length=424, Percent_Identity=27.122641509434, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1789941, Length=388, Percent_Identity=28.6082474226804, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87082404, Length=340, Percent_Identity=26.4705882352941, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87082231, Length=407, Percent_Identity=23.5872235872236, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47645; Mature: 47514

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAITPQPQNVESGSSKGIREEKNFVPGIPMAQLRSGAIGNLIEWFDWNAYALLSVYFAA
CCCCCCCCCCCCCCCCCCCCHHCCCCCCCCHHHHCCCCHHHHHHHHCCCHHHHHHHHHHH
QFFPAGTSPLVALLGSFGIMAVGFIVRPLAGLAIGVIADRFGRKTAMLITVYGMGISSLM
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHH
IALSPTYAQIGIFAPFILLIARIIQGICIGGEYAALTAFAMEMSPNGKRGLVAAILNIVA
HHHCCCHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
NFGQILVVVIILAFSWSLPDDAMHTWGWRLVFIIGALLSLLGIWMRRDMKETIDVDTSRA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KKESVFAAVKRYPRQTIQIIGLTAGFTAMVYAWGAYMPTYAATYKGFDPKYSIMSMGASF
HHHHHHHHHHHCCHHHEEEEEEHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHCHHH
AVAMVSTYFAGKASDRFGRKPTMIIAGILLSVGTVPALTLLNDSVWTLIIIQSVGLSIIG
HHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHH
MLQSSSMPVFAEMFPSDFRASGLGFPYALTVGLIGGTVPLVGTQLANMNLTSVFPWYLTA
HHHCCCCCHHHHHCCCHHHHCCCCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHH
LIVISTLFYIGMKETAFKPLPK
HHHHHHHHHHCCHHHCCCCCCC
>Mature Secondary Structure 
TAITPQPQNVESGSSKGIREEKNFVPGIPMAQLRSGAIGNLIEWFDWNAYALLSVYFAA
CCCCCCCCCCCCCCCCCCCHHCCCCCCCCHHHHCCCCHHHHHHHHCCCHHHHHHHHHHH
QFFPAGTSPLVALLGSFGIMAVGFIVRPLAGLAIGVIADRFGRKTAMLITVYGMGISSLM
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHH
IALSPTYAQIGIFAPFILLIARIIQGICIGGEYAALTAFAMEMSPNGKRGLVAAILNIVA
HHHCCCHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
NFGQILVVVIILAFSWSLPDDAMHTWGWRLVFIIGALLSLLGIWMRRDMKETIDVDTSRA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KKESVFAAVKRYPRQTIQIIGLTAGFTAMVYAWGAYMPTYAATYKGFDPKYSIMSMGASF
HHHHHHHHHHHCCHHHEEEEEEHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHCHHH
AVAMVSTYFAGKASDRFGRKPTMIIAGILLSVGTVPALTLLNDSVWTLIIIQSVGLSIIG
HHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHH
MLQSSSMPVFAEMFPSDFRASGLGFPYALTVGLIGGTVPLVGTQLANMNLTSVFPWYLTA
HHHCCCCCHHHHHCCCHHHHCCCCCHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHH
LIVISTLFYIGMKETAFKPLPK
HHHHHHHHHHCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]