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The map label for this gene is cysNC [H]

Identifier: 145296797

GI number: 145296797

Start: 2990433

End: 2991734

Strand: Reverse

Name: cysNC [H]

Synonym: cgR_2700

Alternate gene names: 145296797

Gene position: 2991734-2990433 (Counterclockwise)

Preceding gene: 145296798

Following gene: 145296796

Centisome position: 90.27

GC content: 59.37

Gene sequence:

>1302_bases
ATGACTGCTCCAACCTTGAATAAAGCATCCGAAAAGATTGCATCACGCGAGACCCTTCGTCTGTGCACCGCAGGTTCCGT
AGATGATGGCAAGTCCACCTTCGTTGGTCGCCTTCTCCACGACACCAAGTCTGTTCTTGCTGATCAGCTGGCTTCCGTAG
AGCGCACCTCCGCCGACCGTGGCTTCGAAGGCCTCGACCTGTCACTCCTCGTTGACGGCCTTCGCGCCGAGCGTGAGCAG
GGCATCACCATCGACGTTGCCTACCGCTACTTCGCCACCGACAAGCGCACCTTCATCCTGGCTGATACCCCAGGTCACGT
GCAGTACACCCGCAACACCGTCACCGGCGTCTCCACCTCCCAGGTTGTAGTTTTGCTTGTCGACGCCCGCCACGGCGTCG
TCGAGCAGACCCGCCGTCACCTGTCCGTATCGGCCCTGCTGGGCGTGCGCACGGTGATCCTCGCAGTCAACAAAATTGAC
CTTGTTGATTACAGCGAAGAAGTCTTCCGCAACATTGAAAAGGAATTCGTCGGACTGGCATCTGCGCTCGATGTCACTGA
CACCCACGTCGTTCCAATCTCTGCGCTCAAGGGCGACAACGTTGCAGAACCTTCCACCCACATGGATTGGTACACCGGAC
CAACCGTGCTGGAAATTCTGGAAAACGTGGAAGTTTCCCGTGGCCGTGCACACGACCTGGGCTTCCGCTTCCCAATCCAG
TACGTCATCCGTGAGCACGCCTCCGACTACCGTGGCTACGCCGGCACCATCAACGCTGGTTCCGTCTCCGTGGGCGATAC
CGTGTACCTACCTGAAGGCCGCACCACCCAAGTCACCCACATCGATTCCGCTGACGGATCCCTCCAGACCGCATCAGTTG
GAGATGCCGTTGTCCTGCGCCTCGCTCAGGAAATCGACCTCATCCGCGGCGAACTCATCGCTGGCGAAGACCGCCCAGAA
TCCGTTCGCTCCTTCAACGCCACCGTTGTTGGCTTGGCCGATCGCACCATCAAACCAGGTGCAGCAGTCAAGGTCCGCTA
CGGCACCGAGCTGGTCCGCGGACGCGTCGCAGCCATCGAACGAGTCCTCGACATCGACGGCGTCAACGACAACGAAGCAC
CAGAAACCTATGGCCTCAACGACATCGCACACGTGCGCATTGACGTTGCAGGCGAACTCGAAGTTGAAGATTACGCTGCC
CGCGGCGCCATCGGATCCTTCCTCCTCATCGACCAATCCTCCGGCGATACCCTCGCTGCTGGCTTGGTTGGCCACCGCCT
ACGCAATAACTGGTCGATCTAA

Upstream 100 bases:

>100_bases
AGATCGCCACCTCCACCCTTACCGAACGTGGCGCAACCCGCGCCGATGACCGCCTCAGCGAATCCGCAATGGAAGACCGC
AAGAAGGAAGGCTACTTCTG

Downstream 100 bases:

>100_bases
ACCAGTTGCTGTGAAGGCAAGGTGTCGGCTTAGATGCCGGCGCCTAGCCTACATCCAGCTAAGACCCCCTTTAGGACACC
TCATGATTCCCCTGATTACG

Product: hypothetical protein

Products: NA

Alternate protein names: Sulfate adenylyltransferase subunit 1; ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; APS kinase; ATP adenosine-5'-phosphosulfate 3'-phosphotransferase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTAPTLNKASEKIASRETLRLCTAGSVDDGKSTFVGRLLHDTKSVLADQLASVERTSADRGFEGLDLSLLVDGLRAEREQ
GITIDVAYRYFATDKRTFILADTPGHVQYTRNTVTGVSTSQVVVLLVDARHGVVEQTRRHLSVSALLGVRTVILAVNKID
LVDYSEEVFRNIEKEFVGLASALDVTDTHVVPISALKGDNVAEPSTHMDWYTGPTVLEILENVEVSRGRAHDLGFRFPIQ
YVIREHASDYRGYAGTINAGSVSVGDTVYLPEGRTTQVTHIDSADGSLQTASVGDAVVLRLAQEIDLIRGELIAGEDRPE
SVRSFNATVVGLADRTIKPGAAVKVRYGTELVRGRVAAIERVLDIDGVNDNEAPETYGLNDIAHVRIDVAGELEVEDYAA
RGAIGSFLLIDQSSGDTLAAGLVGHRLRNNWSI

Sequences:

>Translated_433_residues
MTAPTLNKASEKIASRETLRLCTAGSVDDGKSTFVGRLLHDTKSVLADQLASVERTSADRGFEGLDLSLLVDGLRAEREQ
GITIDVAYRYFATDKRTFILADTPGHVQYTRNTVTGVSTSQVVVLLVDARHGVVEQTRRHLSVSALLGVRTVILAVNKID
LVDYSEEVFRNIEKEFVGLASALDVTDTHVVPISALKGDNVAEPSTHMDWYTGPTVLEILENVEVSRGRAHDLGFRFPIQ
YVIREHASDYRGYAGTINAGSVSVGDTVYLPEGRTTQVTHIDSADGSLQTASVGDAVVLRLAQEIDLIRGELIAGEDRPE
SVRSFNATVVGLADRTIKPGAAVKVRYGTELVRGRVAAIERVLDIDGVNDNEAPETYGLNDIAHVRIDVAGELEVEDYAA
RGAIGSFLLIDQSSGDTLAAGLVGHRLRNNWSI
>Mature_432_residues
TAPTLNKASEKIASRETLRLCTAGSVDDGKSTFVGRLLHDTKSVLADQLASVERTSADRGFEGLDLSLLVDGLRAEREQG
ITIDVAYRYFATDKRTFILADTPGHVQYTRNTVTGVSTSQVVVLLVDARHGVVEQTRRHLSVSALLGVRTVILAVNKIDL
VDYSEEVFRNIEKEFVGLASALDVTDTHVVPISALKGDNVAEPSTHMDWYTGPTVLEILENVEVSRGRAHDLGFRFPIQY
VIREHASDYRGYAGTINAGSVSVGDTVYLPEGRTTQVTHIDSADGSLQTASVGDAVVLRLAQEIDLIRGELIAGEDRPES
VRSFNATVVGLADRTIKPGAAVKVRYGTELVRGRVAAIERVLDIDGVNDNEAPETYGLNDIAHVRIDVAGELEVEDYAAR
GAIGSFLLIDQSSGDTLAAGLVGHRLRNNWSI

Specific function: APS kinase catalyzes the synthesis of activated sulfate [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the APS kinase family [H]

Homologues:

Organism=Homo sapiens, GI194018520, Length=432, Percent_Identity=27.0833333333333, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI194097354, Length=432, Percent_Identity=27.0833333333333, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI194018522, Length=432, Percent_Identity=27.0833333333333, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI4503475, Length=440, Percent_Identity=27.7272727272727, Blast_Score=141, Evalue=9e-34,
Organism=Homo sapiens, GI223555963, Length=431, Percent_Identity=28.538283062645, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI5729864, Length=431, Percent_Identity=28.538283062645, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI46094014, Length=432, Percent_Identity=26.6203703703704, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI4503471, Length=440, Percent_Identity=28.1818181818182, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI34147630, Length=319, Percent_Identity=28.5266457680251, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI157426893, Length=180, Percent_Identity=32.2222222222222, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789108, Length=413, Percent_Identity=46.9733656174334, Blast_Score=352, Evalue=3e-98,
Organism=Escherichia coli, GI2367247, Length=350, Percent_Identity=24.5714285714286, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790412, Length=147, Percent_Identity=29.2517006802721, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789737, Length=147, Percent_Identity=29.2517006802721, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI32566629, Length=432, Percent_Identity=28.4722222222222, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI115532065, Length=452, Percent_Identity=27.6548672566372, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=452, Percent_Identity=27.6548672566372, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17552884, Length=339, Percent_Identity=30.6784660766962, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17569207, Length=339, Percent_Identity=30.6784660766962, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI32566303, Length=338, Percent_Identity=27.810650887574, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=136, Percent_Identity=33.8235294117647, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25141371, Length=141, Percent_Identity=31.2056737588652, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17556456, Length=153, Percent_Identity=29.4117647058824, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6325337, Length=339, Percent_Identity=31.858407079646, Blast_Score=149, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6319594, Length=339, Percent_Identity=31.858407079646, Blast_Score=149, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6322937, Length=404, Percent_Identity=28.4653465346535, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6320377, Length=435, Percent_Identity=27.3563218390805, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324761, Length=162, Percent_Identity=30.2469135802469, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24652838, Length=440, Percent_Identity=28.8636363636364, Blast_Score=147, Evalue=9e-36,
Organism=Drosophila melanogaster, GI17137572, Length=440, Percent_Identity=28.8636363636364, Blast_Score=147, Evalue=9e-36,
Organism=Drosophila melanogaster, GI45553807, Length=440, Percent_Identity=27.2727272727273, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI45553816, Length=440, Percent_Identity=27.2727272727273, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24651721, Length=440, Percent_Identity=27.2727272727273, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17864154, Length=440, Percent_Identity=27.2727272727273, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI45550900, Length=432, Percent_Identity=27.0833333333333, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI17137380, Length=437, Percent_Identity=26.0869565217391, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI19921738, Length=176, Percent_Identity=30.6818181818182, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281363316, Length=332, Percent_Identity=26.2048192771084, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17864358, Length=332, Percent_Identity=26.2048192771084, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922690, Length=292, Percent_Identity=27.3972602739726, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002891
- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF01583 APS_kinase; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4; =2.7.1.25 [H]

Molecular weight: Translated: 46863; Mature: 46732

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPTLNKASEKIASRETLRLCTAGSVDDGKSTFVGRLLHDTKSVLADQLASVERTSADR
CCCCCCCHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GFEGLDLSLLVDGLRAEREQGITIDVAYRYFATDKRTFILADTPGHVQYTRNTVTGVSTS
CCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCEEEEEECCCCCEEEECCEECCCCCC
QVVVLLVDARHGVVEQTRRHLSVSALLGVRTVILAVNKIDLVDYSEEVFRNIEKEFVGLA
EEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEECCEEEECCHHHHHHHHHHHHHHHH
SALDVTDTHVVPISALKGDNVAEPSTHMDWYTGPTVLEILENVEVSRGRAHDLGFRFPIQ
HHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCHHCCCCCCCCCCCCHHH
YVIREHASDYRGYAGTINAGSVSVGDTVYLPEGRTTQVTHIDSADGSLQTASVGDAVVLR
HHHHHHHHHCCCCEEEECCCCEECCCEEECCCCCEEEEEEECCCCCCEEECCCCHHHHHH
LAQEIDLIRGELIAGEDRPESVRSFNATVVGLADRTIKPGAAVKVRYGTELVRGRVAAIE
HHHHHHHHHCEEECCCCCCHHHHHCCCEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHH
RVLDIDGVNDNEAPETYGLNDIAHVRIDVAGELEVEDYAARGAIGSFLLIDQSSGDTLAA
HHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCEEHHHHHHCCCCCEEEEEECCCCCEEHH
GLVGHRLRNNWSI
HHHHHHHHCCCCC
>Mature Secondary Structure 
TAPTLNKASEKIASRETLRLCTAGSVDDGKSTFVGRLLHDTKSVLADQLASVERTSADR
CCCCCCHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GFEGLDLSLLVDGLRAEREQGITIDVAYRYFATDKRTFILADTPGHVQYTRNTVTGVSTS
CCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCEEEEEECCCCCEEEECCEECCCCCC
QVVVLLVDARHGVVEQTRRHLSVSALLGVRTVILAVNKIDLVDYSEEVFRNIEKEFVGLA
EEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEECCEEEECCHHHHHHHHHHHHHHHH
SALDVTDTHVVPISALKGDNVAEPSTHMDWYTGPTVLEILENVEVSRGRAHDLGFRFPIQ
HHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCHHCCCCCCCCCCCCHHH
YVIREHASDYRGYAGTINAGSVSVGDTVYLPEGRTTQVTHIDSADGSLQTASVGDAVVLR
HHHHHHHHHCCCCEEEECCCCEECCCEEECCCCCEEEEEEECCCCCCEEECCCCHHHHHH
LAQEIDLIRGELIAGEDRPESVRSFNATVVGLADRTIKPGAAVKVRYGTELVRGRVAAIE
HHHHHHHHHCEEECCCCCCHHHHHCCCEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHH
RVLDIDGVNDNEAPETYGLNDIAHVRIDVAGELEVEDYAARGAIGSFLLIDQSSGDTLAA
HHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCEEHHHHHHCCCCCEEEEEECCCCCEEHH
GLVGHRLRNNWSI
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]