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The map label for this gene is mmcV [H]

Identifier: 145296798

GI number: 145296798

Start: 2991734

End: 2992648

Strand: Reverse

Name: mmcV [H]

Synonym: cgR_2701

Alternate gene names: 145296798

Gene position: 2992648-2991734 (Counterclockwise)

Preceding gene: 145296799

Following gene: 145296797

Centisome position: 90.3

GC content: 60.33

Gene sequence:

>915_bases
ATGACCACAACCGTTGCATCAGACCTATCCCCACACCTTAAAGATCTTGAAAATGAATCCATCCACATCCTCCGCGAGGT
AGCTGGCCAGTTTGATAAGGTCGGCCTGCTGTTTTCCGGCGGTAAGGATTCCGTCGTGGTGTACGAGCTTGCGCGCCGCG
CTTTCGCTCCAGCTACCGTGCCTTTTGAATTGCTGCACGTGGACACCGGCCACAACTTCCCAGAGGTTTTGGAATTCCGT
GACAACCTGGTGGAGCGCACCGGCGCCCGCCTGCGCGTAGCTAAAGTCCAGGACTGGATCGACCGCGGTGACCTGCAGGA
ACGCCCAGACGGCACCCGCAACCCACTGCAGACTGTCCCTTTGGTGGAGACCATCGCTGAGCAGGGCTACGACGCCGTGC
TTGGTGGCGCTCGCCGCGATGAGGAGCGTGCCCGCGCCAAGGAGCGTGTGTTCTCTGTGCGTGACTCCTTCGGTGGTTGG
GATCCACGCCGTCAGCGCCCAGAGCTGTGGACCCTCTACAACGGTGGCCACCTGCCAGGCGAAAACATCCGTGTTTTCCC
AATCTCCAACTGGACTGAAGCTGACATTTGGGAGTACATCGGCGCCCGCGGCATCGAACTTCCACCGATCTACTTCTCCC
ACGACCGCGAAGTTTTCGAGCGCGACGGCATGTGGCTGACCGCAGGCGAGTGGGGTGGACCAAAGAAGGGCGAGGAAATC
GTCACCAAGACTGTCCGCTACCGCACCGTCGGCGATATGTCCTGCACCGGTGCTGTGCTCTCCGAAGCCCGCACCATTGA
CGATGTGATCGAAGAGATCGCCACCTCCACCCTTACCGAACGTGGCGCAACCCGCGCCGATGACCGCCTCAGCGAATCCG
CAATGGAAGACCGCAAGAAGGAAGGCTACTTCTGA

Upstream 100 bases:

>100_bases
CAATTGGATGCGAAACCTGCACCCTTCCCGTTGCTGAAGGACAAGACCCTAGGGCCGGCCGTTGGGCTGGAAACGCCAAG
ACAGAATGCGGACTTCACTC

Downstream 100 bases:

>100_bases
TGACTGCTCCAACCTTGAATAAAGCATCCGAAAAGATTGCATCACGCGAGACCCTTCGTCTGTGCACCGCAGGTTCCGTA
GATGATGGCAAGTCCACCTT

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Mitomycin biosynthesis protein V; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MTTTVASDLSPHLKDLENESIHILREVAGQFDKVGLLFSGGKDSVVVYELARRAFAPATVPFELLHVDTGHNFPEVLEFR
DNLVERTGARLRVAKVQDWIDRGDLQERPDGTRNPLQTVPLVETIAEQGYDAVLGGARRDEERARAKERVFSVRDSFGGW
DPRRQRPELWTLYNGGHLPGENIRVFPISNWTEADIWEYIGARGIELPPIYFSHDREVFERDGMWLTAGEWGGPKKGEEI
VTKTVRYRTVGDMSCTGAVLSEARTIDDVIEEIATSTLTERGATRADDRLSESAMEDRKKEGYF

Sequences:

>Translated_304_residues
MTTTVASDLSPHLKDLENESIHILREVAGQFDKVGLLFSGGKDSVVVYELARRAFAPATVPFELLHVDTGHNFPEVLEFR
DNLVERTGARLRVAKVQDWIDRGDLQERPDGTRNPLQTVPLVETIAEQGYDAVLGGARRDEERARAKERVFSVRDSFGGW
DPRRQRPELWTLYNGGHLPGENIRVFPISNWTEADIWEYIGARGIELPPIYFSHDREVFERDGMWLTAGEWGGPKKGEEI
VTKTVRYRTVGDMSCTGAVLSEARTIDDVIEEIATSTLTERGATRADDRLSESAMEDRKKEGYF
>Mature_303_residues
TTTVASDLSPHLKDLENESIHILREVAGQFDKVGLLFSGGKDSVVVYELARRAFAPATVPFELLHVDTGHNFPEVLEFRD
NLVERTGARLRVAKVQDWIDRGDLQERPDGTRNPLQTVPLVETIAEQGYDAVLGGARRDEERARAKERVFSVRDSFGGWD
PRRQRPELWTLYNGGHLPGENIRVFPISNWTEADIWEYIGARGIELPPIYFSHDREVFERDGMWLTAGEWGGPKKGEEIV
TKTVRYRTVGDMSCTGAVLSEARTIDDVIEEIATSTLTERGATRADDRLSESAMEDRKKEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=297, Percent_Identity=47.4747474747475, Blast_Score=258, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 34351; Mature: 34220

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTVASDLSPHLKDLENESIHILREVAGQFDKVGLLFSGGKDSVVVYELARRAFAPATV
CCCCCCCCCCHHHHHCCCCHHHHHHHHHCCHHCEEEEEECCCCCCHHHHHHHHHCCCCCC
PFELLHVDTGHNFPEVLEFRDNLVERTGARLRVAKVQDWIDRGDLQERPDGTRNPLQTVP
CEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCHHHH
LVETIAEQGYDAVLGGARRDEERARAKERVFSVRDSFGGWDPRRQRPELWTLYNGGHLPG
HHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCC
ENIRVFPISNWTEADIWEYIGARGIELPPIYFSHDREVFERDGMWLTAGEWGGPKKGEEI
CCEEEEECCCCCHHHHHHHHCCCCCCCCCEEECCCHHHHHHCCCEEEECCCCCCCCCHHH
VTKTVRYRTVGDMSCTGAVLSEARTIDDVIEEIATSTLTERGATRADDRLSESAMEDRKK
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EGYF
CCCC
>Mature Secondary Structure 
TTTVASDLSPHLKDLENESIHILREVAGQFDKVGLLFSGGKDSVVVYELARRAFAPATV
CCCCCCCCCHHHHHCCCCHHHHHHHHHCCHHCEEEEEECCCCCCHHHHHHHHHCCCCCC
PFELLHVDTGHNFPEVLEFRDNLVERTGARLRVAKVQDWIDRGDLQERPDGTRNPLQTVP
CEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCHHHH
LVETIAEQGYDAVLGGARRDEERARAKERVFSVRDSFGGWDPRRQRPELWTLYNGGHLPG
HHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCC
ENIRVFPISNWTEADIWEYIGARGIELPPIYFSHDREVFERDGMWLTAGEWGGPKKGEEI
CCEEEEECCCCCHHHHHHHHCCCCCCCCCEEECCCHHHHHHCCCEEEECCCCCCCCCHHH
VTKTVRYRTVGDMSCTGAVLSEARTIDDVIEEIATSTLTERGATRADDRLSESAMEDRKK
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EGYF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10099135 [H]