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The map label for this gene is lmrB [H]

Identifier: 145296681

GI number: 145296681

Start: 2857561

End: 2859006

Strand: Reverse

Name: lmrB [H]

Synonym: cgR_2586

Alternate gene names: 145296681

Gene position: 2859006-2857561 (Counterclockwise)

Preceding gene: 145296682

Following gene: 145296680

Centisome position: 86.27

GC content: 52.07

Gene sequence:

>1446_bases
ATGGATTCCCAAATTAATACTCAGACCTCTCCGGCAACTGCGAAGCTGCCTAGGGAGGTCGTTGTTGTTCTTTCGATCCT
CGTGATTTCTGCGATGATCATGATTCTTAATGAAACCATTCTGTCAGTTGCGTTGCCGTCGATCATGGCGGATTTCCAGG
TTCCAGAAACTACTGCGCAGTGGTTGACCACTGGCTTTATGTTGACGATGGCAGTGGTGATTCCTACTACTGGTTATTTG
CTTGATCGTTTTTCCACTAAGACGATCTTTGTTACTGCGTTGTTGTTCTTTACTGTTGGTACCTTGACTGCGGCGTTGGC
CCCAACGTTTGCTGTGCTGCTTGGTGCTCGTATCATTCAGGCGGTTGGTACTGCGCTGGTGATGCCTTTGCTGATGACAG
TTACGTTGACGGTGGTTCCTGCGGAGCGTCGTGGTTCGATGATGGGCATTATTTCCATCGTGATTTCTGTTGCGCCGGCG
CTTGGTCCTACTTTGTCTGGTGTCATTCTTAACTCTTTGACCTGGCACTGGTTGTTTTGGATGATGCTTCCGATCGTGGC
CGTTGCGCTGGTTATTGGCTTCTTTTTGATCAAAAATATCGGCGAAACCAAGATCACCCCACTGGATGTTCTGTCTGTCA
TTCTTTCAGTGTTTGCCTTTGGCGGTTTGGTGTACGGTTTCAGTTCCTTCGGAGCAATCCTGGAGGGTAAGGGCGTAGTA
GGTATCGTCGCGATCGTCGTTGGTGCCATCGCACTTGTTATCTTTGCTTTACGACAGCACCAACTCGGCAAGCAAGACAA
GGCACTGATGGATCTCCGCGCCTTCAAGGTGAGGAACTTCAGCTTCTCTTTGACCACCATTCTTTTGGCTTTCGGCGCGA
TGCTCGGAACCGTCATGGTTTTGCCCATCTACCTGCAGACTTCCCTCGGAGTTACCGCTTTGGTGACCGGCTTGGTTGTC
ATGCCAGGTGGCCTCCTCCAGGGTCTGATCAGCCCATTCATCGGCCGTTTCTACGACAAGGTCGGTCCACGTCCGTTGCT
GATTCCCGGCGCAATTGCGCTGGCTGTCGCGGCATCGTCGATGACTTTCCTCAATGAGAATTCACCCGTGTGGATGGTTG
TTGTCCTGCACGTTATCTTCAGCATCGGCATGTGTTTGATGATGACCCCGCTCATGACCACCGCTCTCGGCGCACTTCCG
AAGCACCTTTATGGTCACGGCTCCGCAATTTTGAACACGTTCCAGCAGCTCGCAGGCGCAGCAGGAACAGCGATTATGAT
TGCAGCACTTTCCTTCGGCACTTCCATTGCAGCGTCTTCCGGATCTGCACACGCCGAAGCTGTCGCCGCTGGTACCAGGG
TTGCGTTCATCGCAGGCGCAATCATCGCAGTGATCGCTTTGGTTGTTTCCCTCTTCGTCACTCGCGTCGAGGAAGAAACT
CACTAA

Upstream 100 bases:

>100_bases
ATGTACTTGCTTATGGCACTCTTTAGGAGTTTTTTCTCATAGCTCAGTTTCGTAACACTAGAAACTGAGCTTCATGCTGT
GAAAGGCTTTTTCTCCATTC

Downstream 100 bases:

>100_bases
ACACCAAGAATTGGGCAGAAGGTCATTAACTTTCTGCCCAGTTTTTTGCTTTTCGACGCCTACCTCCAGCGCACCTTGCA
GGTTGCTGCGGATCGCTGAA

Product: hypothetical protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MDSQINTQTSPATAKLPREVVVVLSILVISAMIMILNETILSVALPSIMADFQVPETTAQWLTTGFMLTMAVVIPTTGYL
LDRFSTKTIFVTALLFFTVGTLTAALAPTFAVLLGARIIQAVGTALVMPLLMTVTLTVVPAERRGSMMGIISIVISVAPA
LGPTLSGVILNSLTWHWLFWMMLPIVAVALVIGFFLIKNIGETKITPLDVLSVILSVFAFGGLVYGFSSFGAILEGKGVV
GIVAIVVGAIALVIFALRQHQLGKQDKALMDLRAFKVRNFSFSLTTILLAFGAMLGTVMVLPIYLQTSLGVTALVTGLVV
MPGGLLQGLISPFIGRFYDKVGPRPLLIPGAIALAVAASSMTFLNENSPVWMVVVLHVIFSIGMCLMMTPLMTTALGALP
KHLYGHGSAILNTFQQLAGAAGTAIMIAALSFGTSIAASSGSAHAEAVAAGTRVAFIAGAIIAVIALVVSLFVTRVEEET
H

Sequences:

>Translated_481_residues
MDSQINTQTSPATAKLPREVVVVLSILVISAMIMILNETILSVALPSIMADFQVPETTAQWLTTGFMLTMAVVIPTTGYL
LDRFSTKTIFVTALLFFTVGTLTAALAPTFAVLLGARIIQAVGTALVMPLLMTVTLTVVPAERRGSMMGIISIVISVAPA
LGPTLSGVILNSLTWHWLFWMMLPIVAVALVIGFFLIKNIGETKITPLDVLSVILSVFAFGGLVYGFSSFGAILEGKGVV
GIVAIVVGAIALVIFALRQHQLGKQDKALMDLRAFKVRNFSFSLTTILLAFGAMLGTVMVLPIYLQTSLGVTALVTGLVV
MPGGLLQGLISPFIGRFYDKVGPRPLLIPGAIALAVAASSMTFLNENSPVWMVVVLHVIFSIGMCLMMTPLMTTALGALP
KHLYGHGSAILNTFQQLAGAAGTAIMIAALSFGTSIAASSGSAHAEAVAAGTRVAFIAGAIIAVIALVVSLFVTRVEEET
H
>Mature_481_residues
MDSQINTQTSPATAKLPREVVVVLSILVISAMIMILNETILSVALPSIMADFQVPETTAQWLTTGFMLTMAVVIPTTGYL
LDRFSTKTIFVTALLFFTVGTLTAALAPTFAVLLGARIIQAVGTALVMPLLMTVTLTVVPAERRGSMMGIISIVISVAPA
LGPTLSGVILNSLTWHWLFWMMLPIVAVALVIGFFLIKNIGETKITPLDVLSVILSVFAFGGLVYGFSSFGAILEGKGVV
GIVAIVVGAIALVIFALRQHQLGKQDKALMDLRAFKVRNFSFSLTTILLAFGAMLGTVMVLPIYLQTSLGVTALVTGLVV
MPGGLLQGLISPFIGRFYDKVGPRPLLIPGAIALAVAASSMTFLNENSPVWMVVVLHVIFSIGMCLMMTPLMTTALGALP
KHLYGHGSAILNTFQQLAGAAGTAIMIAALSFGTSIAASSGSAHAEAVAAGTRVAFIAGAIIAVIALVVSLFVTRVEEET
H

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=404, Percent_Identity=26.980198019802, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789042, Length=413, Percent_Identity=28.0871670702179, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI87081983, Length=422, Percent_Identity=26.5402843601896, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1790195, Length=401, Percent_Identity=27.6807980049875, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788392, Length=476, Percent_Identity=25.8403361344538, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1788509, Length=190, Percent_Identity=27.8947368421053, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=286, Percent_Identity=25.5244755244755, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=129, Percent_Identity=32.5581395348837, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6322958, Length=288, Percent_Identity=24.6527777777778, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323735, Length=166, Percent_Identity=27.710843373494, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50546; Mature: 50546

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSQINTQTSPATAKLPREVVVVLSILVISAMIMILNETILSVALPSIMADFQVPETTAQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
WLTTGFMLTMAVVIPTTGYLLDRFSTKTIFVTALLFFTVGTLTAALAPTFAVLLGARIIQ
HHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVGTALVMPLLMTVTLTVVPAERRGSMMGIISIVISVAPALGPTLSGVILNSLTWHWLFW
HHHHHHHHHHHHHHHHHEEEHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
MMLPIVAVALVIGFFLIKNIGETKITPLDVLSVILSVFAFGGLVYGFSSFGAILEGKGVV
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
GIVAIVVGAIALVIFALRQHQLGKQDKALMDLRAFKVRNFSFSLTTILLAFGAMLGTVMV
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LPIYLQTSLGVTALVTGLVVMPGGLLQGLISPFIGRFYDKVGPRPLLIPGAIALAVAASS
HHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHH
MTFLNENSPVWMVVVLHVIFSIGMCLMMTPLMTTALGALPKHLYGHGSAILNTFQQLAGA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
AGTAIMIAALSFGTSIAASSGSAHAEAVAAGTRVAFIAGAIIAVIALVVSLFVTRVEEET
CCHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
H
C
>Mature Secondary Structure
MDSQINTQTSPATAKLPREVVVVLSILVISAMIMILNETILSVALPSIMADFQVPETTAQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
WLTTGFMLTMAVVIPTTGYLLDRFSTKTIFVTALLFFTVGTLTAALAPTFAVLLGARIIQ
HHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVGTALVMPLLMTVTLTVVPAERRGSMMGIISIVISVAPALGPTLSGVILNSLTWHWLFW
HHHHHHHHHHHHHHHHHEEEHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
MMLPIVAVALVIGFFLIKNIGETKITPLDVLSVILSVFAFGGLVYGFSSFGAILEGKGVV
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
GIVAIVVGAIALVIFALRQHQLGKQDKALMDLRAFKVRNFSFSLTTILLAFGAMLGTVMV
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LPIYLQTSLGVTALVTGLVVMPGGLLQGLISPFIGRFYDKVGPRPLLIPGAIALAVAASS
HHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHH
MTFLNENSPVWMVVVLHVIFSIGMCLMMTPLMTTALGALPKHLYGHGSAILNTFQQLAGA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
AGTAIMIAALSFGTSIAASSGSAHAEAVAAGTRVAFIAGAIIAVIALVVSLFVTRVEEET
CCHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
H
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]