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The map label for this gene is radA [H]

Identifier: 145296662

GI number: 145296662

Start: 2835233

End: 2836627

Strand: Direct

Name: radA [H]

Synonym: cgR_2569

Alternate gene names: 145296662

Gene position: 2835233-2836627 (Clockwise)

Preceding gene: 145296661

Following gene: 145296663

Centisome position: 85.55

GC content: 58.06

Gene sequence:

>1395_bases
ATGGCAAAAAAGGTCCGCAGCGTACACGAGTGTTCTGAATGTGGTTATTCCAGCCCAAAGTGGTTGGGGCGCTGCCCTGA
ATGTGGTTCTTGGGGTTCCATGGAAGAGCGCACAGTCGGTGAGTCCGGTGGTGCGGCTCGTGCTGCTGTGTCGGGTGCTG
CGCCGGCAGGCTTGACCCCAACTTCCCCGGCAGTGCCCATCGGTAAGATCGTTGCTTCCCAGGCCACAAGCATTTCCACT
GGTATTGGAGAGTTAGATCGCGTACTCGGTAACGGCATCGTGCCAGGATCCGTCGTACTCATGGCCGGTGAGCCCGGTGT
TGGTAAATCCACTTTGCTGCTTGAGGTCGCAAGCCGTTGGGCTTCAATGAAAGAGGGCGACGGCACCCGCACCGCACTGT
ATGTCACTGCGGAGGAATCAGCAGGTCAGGTGCGTCTTCGGGCTGAGCGCACCGCAGCTGTCAAAGACACCCTTTATCTT
GCTGCTGAATCCAATCTGGATGTGGTTTTCGGCCACGTTAACCAAGTGAAACCGAGCCTGCTTATCGTAGACTCCGTGCA
AACTATGCATGCCGCTGGCGTTGAAGGCGTTGCCGGCGGTGTCGCGCAATCCCGCGCTGTCACAGCTGCGCTCACCACGC
TTGCGAAAACCTCCGGAATCCCAATCCTGCTGGTCGGCCACGTCACAAAGGACGGCAATGTTGCAGGTCCACGCGTCCTC
GAACACCTCGTTGACGTCGTTTTAAACTTCGAAGGTGACCGCCACTCCTCCCTAAGAATGCTGCGCGGCATCAAAAACCG
CTTCGGCGCCACCGATGAAGTTGGCTGCTTCGAACAACAATCCGACGGTATCAAAGAAGTCCCCGACCCCTCCGGTCTCT
TCCTCTCCCACCGCGGCTCAACCCCCGACGGCACCGCTGTCACCGTCGCCATGGATGGCGTGCGCCCGCTGTTAGCGGAG
GTCCAAAGCCTGCTTGTCGACGCCCCCTCCAAGAATCCACGCAGGGTCGTCACGGGCCTCGACGCCAATCGGGTCCCCAT
GGTCCTTGCGGTATTATCTGCCCGTGCGGGTCGCCAAACCCAAGGAAAGGATGCCTACGTGGCAACAGTCGGAGGAATGA
AAGTGGGTGAACCTGCAACAGACCTCGCTGTCGCGCTCGCTACTGCCTCTGCTTTAGCCAAGAAACCACTACCAGATAAA
ACAGTAGTGCTCGGTGAAGTCGGCTTGGCCGGAGAAATCCGCCGCGTCCCCAACGTTGATCGCAGACTAGCCGAGGCGGA
ACGTTTAGGTTATGAAAAAGCAGTCATCCCCGGAGGCTCAGGCATCAAACAAACAAGCCTGAGAGTCATCGAAGCTTCAA
CGCTTGCCGAAGCACTTGCAGCAGTAAGCTTATAA

Upstream 100 bases:

>100_bases
CCGCCCACTTCCAATGAATTGTTGGAGGTGGGCGGTTATTTTTCACCCCTTTTCGAACACCGTTTCCCTTAATGTCCACC
CCACCGTTTAAGGTAGGGGC

Downstream 100 bases:

>100_bases
ACCATGACACCAACAACCACTCCTGTATCAAACCCAGATGCCCTTGCCCCTGGAACTCAGGATGTGCACACCCTCAAAGG
AACGCTACAGCGCCTCGCCC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MAKKVRSVHECSECGYSSPKWLGRCPECGSWGSMEERTVGESGGAARAAVSGAAPAGLTPTSPAVPIGKIVASQATSIST
GIGELDRVLGNGIVPGSVVLMAGEPGVGKSTLLLEVASRWASMKEGDGTRTALYVTAEESAGQVRLRAERTAAVKDTLYL
AAESNLDVVFGHVNQVKPSLLIVDSVQTMHAAGVEGVAGGVAQSRAVTAALTTLAKTSGIPILLVGHVTKDGNVAGPRVL
EHLVDVVLNFEGDRHSSLRMLRGIKNRFGATDEVGCFEQQSDGIKEVPDPSGLFLSHRGSTPDGTAVTVAMDGVRPLLAE
VQSLLVDAPSKNPRRVVTGLDANRVPMVLAVLSARAGRQTQGKDAYVATVGGMKVGEPATDLAVALATASALAKKPLPDK
TVVLGEVGLAGEIRRVPNVDRRLAEAERLGYEKAVIPGGSGIKQTSLRVIEASTLAEALAAVSL

Sequences:

>Translated_464_residues
MAKKVRSVHECSECGYSSPKWLGRCPECGSWGSMEERTVGESGGAARAAVSGAAPAGLTPTSPAVPIGKIVASQATSIST
GIGELDRVLGNGIVPGSVVLMAGEPGVGKSTLLLEVASRWASMKEGDGTRTALYVTAEESAGQVRLRAERTAAVKDTLYL
AAESNLDVVFGHVNQVKPSLLIVDSVQTMHAAGVEGVAGGVAQSRAVTAALTTLAKTSGIPILLVGHVTKDGNVAGPRVL
EHLVDVVLNFEGDRHSSLRMLRGIKNRFGATDEVGCFEQQSDGIKEVPDPSGLFLSHRGSTPDGTAVTVAMDGVRPLLAE
VQSLLVDAPSKNPRRVVTGLDANRVPMVLAVLSARAGRQTQGKDAYVATVGGMKVGEPATDLAVALATASALAKKPLPDK
TVVLGEVGLAGEIRRVPNVDRRLAEAERLGYEKAVIPGGSGIKQTSLRVIEASTLAEALAAVSL
>Mature_463_residues
AKKVRSVHECSECGYSSPKWLGRCPECGSWGSMEERTVGESGGAARAAVSGAAPAGLTPTSPAVPIGKIVASQATSISTG
IGELDRVLGNGIVPGSVVLMAGEPGVGKSTLLLEVASRWASMKEGDGTRTALYVTAEESAGQVRLRAERTAAVKDTLYLA
AESNLDVVFGHVNQVKPSLLIVDSVQTMHAAGVEGVAGGVAQSRAVTAALTTLAKTSGIPILLVGHVTKDGNVAGPRVLE
HLVDVVLNFEGDRHSSLRMLRGIKNRFGATDEVGCFEQQSDGIKEVPDPSGLFLSHRGSTPDGTAVTVAMDGVRPLLAEV
QSLLVDAPSKNPRRVVTGLDANRVPMVLAVLSARAGRQTQGKDAYVATVGGMKVGEPATDLAVALATASALAKKPLPDKT
VVLGEVGLAGEIRRVPNVDRRLAEAERLGYEKAVIPGGSGIKQTSLRVIEASTLAEALAAVSL

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=463, Percent_Identity=41.4686825053996, Blast_Score=357, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 47863; Mature: 47732

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKVRSVHECSECGYSSPKWLGRCPECGSWGSMEERTVGESGGAARAAVSGAAPAGLTP
CCHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCHHHHCCCCCCCHHHEECCCCCCCCCC
TSPAVPIGKIVASQATSISTGIGELDRVLGNGIVPGSVVLMAGEPGVGKSTLLLEVASRW
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
ASMKEGDGTRTALYVTAEESAGQVRLRAERTAAVKDTLYLAAESNLDVVFGHVNQVKPSL
HHCCCCCCCEEEEEEEECCCCCEEEEEEHHHHHHHHEEEEEECCCCEEEEECHHHCCCCE
LIVDSVQTMHAAGVEGVAGGVAQSRAVTAALTTLAKTSGIPILLVGHVTKDGNVAGPRVL
EEEECHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHH
EHLVDVVLNFEGDRHSSLRMLRGIKNRFGATDEVGCFEQQSDGIKEVPDPSGLFLSHRGS
HHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCEEEEECCC
TPDGTAVTVAMDGVRPLLAEVQSLLVDAPSKNPRRVVTGLDANRVPMVLAVLSARAGRQT
CCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCC
QGKDAYVATVGGMKVGEPATDLAVALATASALAKKPLPDKTVVLGEVGLAGEIRRVPNVD
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHCCCHH
RRLAEAERLGYEKAVIPGGSGIKQTSLRVIEASTLAEALAAVSL
HHHHHHHHCCCCEEECCCCCCCCHHHEEEEHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKKVRSVHECSECGYSSPKWLGRCPECGSWGSMEERTVGESGGAARAAVSGAAPAGLTP
CHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCHHHHCCCCCCCHHHEECCCCCCCCCC
TSPAVPIGKIVASQATSISTGIGELDRVLGNGIVPGSVVLMAGEPGVGKSTLLLEVASRW
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
ASMKEGDGTRTALYVTAEESAGQVRLRAERTAAVKDTLYLAAESNLDVVFGHVNQVKPSL
HHCCCCCCCEEEEEEEECCCCCEEEEEEHHHHHHHHEEEEEECCCCEEEEECHHHCCCCE
LIVDSVQTMHAAGVEGVAGGVAQSRAVTAALTTLAKTSGIPILLVGHVTKDGNVAGPRVL
EEEECHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHH
EHLVDVVLNFEGDRHSSLRMLRGIKNRFGATDEVGCFEQQSDGIKEVPDPSGLFLSHRGS
HHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCEEEEECCC
TPDGTAVTVAMDGVRPLLAEVQSLLVDAPSKNPRRVVTGLDANRVPMVLAVLSARAGRQT
CCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCC
QGKDAYVATVGGMKVGEPATDLAVALATASALAKKPLPDKTVVLGEVGLAGEIRRVPNVD
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHCCCHH
RRLAEAERLGYEKAVIPGGSGIKQTSLRVIEASTLAEALAAVSL
HHHHHHHHCCCCEEECCCCCCCCHHHEEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]