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The map label for this gene is nagB

Identifier: 145296641

GI number: 145296641

Start: 2815716

End: 2816477

Strand: Reverse

Name: nagB

Synonym: cgR_2549

Alternate gene names: 145296641

Gene position: 2816477-2815716 (Counterclockwise)

Preceding gene: 145296642

Following gene: 145296640

Centisome position: 84.98

GC content: 52.1

Gene sequence:

>762_bases
ATGGACATCATCATCTGCAAAGACGAGCAAGAAGTCGGCAAAGCAGCGGCAGCCCTGATCGCACCCTTCGCAACTAAGGG
CGGAACCTTGGGGCTTGCAACAGGATCGTCACCTTTGAGCACCTACCAAGAGCTCATTCGCATGTATGAAGCTGGGGAAG
TGTCATTCAAGAACTGCAAGGCATTCTTGTTGGATGAATACGTGGGATTAACGCGCGACGATGAAAACAGCTACTTCAAA
ACCATTCGTAAAGAGTTCACTGACCACATCGACATCGTTGATGAAGAGGTCTACAGCCCAGATGGTGCAAACCCTGATCC
ATACGAAGCAGCTGCAGAGTATGAGGCAAAGATCGCTGCAGAATCCGTTGATGTTCAAATCCTTGGCATCGGCGGAAACG
GCCACATCGCTTTCAATGAGCCATCATCTTCTCTGTCAGGACTGACAAAGGTCCAGGCGCTGCACCCTAAAACTGTGGAG
GACAACGCTCGATTCTTCAACACCATCGAAGAGGTCCCAACCCACGCCCTCACCCAGGGTTTGGGCACTTTGTCCCGCGC
GCAAAACATCGTGTTGGTGGCAACTGGTGAAGGAAAAGCCGACGCCATCCGCGGAACTGTGGAAGGCCCAGTGACTGCTT
CTTGCCCAGGTTCCATCCTGCAGATGCACAACAATGCCACCATCATCGTTGATGAAGCAGCAGCATCCAAGCTGGAAAAC
GCTGATCACTACCGTCTCATGGAGCAATTAAAGCTGCGCTAG

Upstream 100 bases:

>100_bases
GTGGTCTTTGACTCAAACGGTCAGTTACAACAGGTCCATTTAGACCATCAAGTAATTTAAATACGAGCAAAACTTTCCTG
ATAATAAAAGGAGTCCGACC

Downstream 100 bases:

>100_bases
AAACAAAAAGGAAAGTACTGTGTGGGGCTATGCACACAGAACTTTCCAGTTTGCGCCCTGCGTACCATGTGACTCCTCCG
CAGGGCAGGCTCAATGATCC

Product: hypothetical protein

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MDIIICKDEQEVGKAAAALIAPFATKGGTLGLATGSSPLSTYQELIRMYEAGEVSFKNCKAFLLDEYVGLTRDDENSYFK
TIRKEFTDHIDIVDEEVYSPDGANPDPYEAAAEYEAKIAAESVDVQILGIGGNGHIAFNEPSSSLSGLTKVQALHPKTVE
DNARFFNTIEEVPTHALTQGLGTLSRAQNIVLVATGEGKADAIRGTVEGPVTASCPGSILQMHNNATIIVDEAAASKLEN
ADHYRLMEQLKLR

Sequences:

>Translated_253_residues
MDIIICKDEQEVGKAAAALIAPFATKGGTLGLATGSSPLSTYQELIRMYEAGEVSFKNCKAFLLDEYVGLTRDDENSYFK
TIRKEFTDHIDIVDEEVYSPDGANPDPYEAAAEYEAKIAAESVDVQILGIGGNGHIAFNEPSSSLSGLTKVQALHPKTVE
DNARFFNTIEEVPTHALTQGLGTLSRAQNIVLVATGEGKADAIRGTVEGPVTASCPGSILQMHNNATIIVDEAAASKLEN
ADHYRLMEQLKLR
>Mature_253_residues
MDIIICKDEQEVGKAAAALIAPFATKGGTLGLATGSSPLSTYQELIRMYEAGEVSFKNCKAFLLDEYVGLTRDDENSYFK
TIRKEFTDHIDIVDEEVYSPDGANPDPYEAAAEYEAKIAAESVDVQILGIGGNGHIAFNEPSSSLSGLTKVQALHPKTVE
DNARFFNTIEEVPTHALTQGLGTLSRAQNIVLVATGEGKADAIRGTVEGPVTASCPGSILQMHNNATIIVDEAAASKLEN
ADHYRLMEQLKLR

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily

Homologues:

Organism=Homo sapiens, GI13027378, Length=248, Percent_Identity=39.5161290322581, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI19923881, Length=213, Percent_Identity=39.4366197183099, Blast_Score=143, Evalue=1e-34,
Organism=Escherichia coli, GI1786893, Length=248, Percent_Identity=38.3064516129032, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI48994958, Length=243, Percent_Identity=26.7489711934156, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789530, Length=219, Percent_Identity=25.1141552511416, Blast_Score=80, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17554876, Length=247, Percent_Identity=40.4858299595142, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24581960, Length=248, Percent_Identity=40.3225806451613, Blast_Score=164, Evalue=4e-41,
Organism=Drosophila melanogaster, GI19920764, Length=248, Percent_Identity=40.3225806451613, Blast_Score=164, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAGB_CORGB (A4QH44)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001139462.1
- ProteinModelPortal:   A4QH44
- SMR:   A4QH44
- STRING:   A4QH44
- GeneID:   4991968
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2549
- eggNOG:   COG0363
- HOGENOM:   HBG725991
- OMA:   HIDIVDE
- ProtClustDB:   CLSK633897
- HAMAP:   MF_01241
- InterPro:   IPR006148
- InterPro:   IPR004547
- PANTHER:   PTHR11280
- TIGRFAMs:   TIGR00502

Pfam domain/function: PF01182 Glucosamine_iso

EC number: =3.5.99.6

Molecular weight: Translated: 27220; Mature: 27220

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: ACT_SITE 65-65 ACT_SITE 133-133 ACT_SITE 135-135 ACT_SITE 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIIICKDEQEVGKAAAALIAPFATKGGTLGLATGSSPLSTYQELIRMYEAGEVSFKNCK
CEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHH
AFLLDEYVGLTRDDENSYFKTIRKEFTDHIDIVDEEVYSPDGANPDPYEAAAEYEAKIAA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEE
ESVDVQILGIGGNGHIAFNEPSSSLSGLTKVQALHPKTVEDNARFFNTIEEVPTHALTQG
CEEEEEEEEECCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
LGTLSRAQNIVLVATGEGKADAIRGTVEGPVTASCPGSILQMHNNATIIVDEAAASKLEN
HHHHHHCCCEEEEEECCCCCHHEECCCCCCEECCCCHHHHEECCCCEEEEECHHHHHHCC
ADHYRLMEQLKLR
CHHHHHHHHHCCC
>Mature Secondary Structure
MDIIICKDEQEVGKAAAALIAPFATKGGTLGLATGSSPLSTYQELIRMYEAGEVSFKNCK
CEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHH
AFLLDEYVGLTRDDENSYFKTIRKEFTDHIDIVDEEVYSPDGANPDPYEAAAEYEAKIAA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEE
ESVDVQILGIGGNGHIAFNEPSSSLSGLTKVQALHPKTVEDNARFFNTIEEVPTHALTQG
CEEEEEEEEECCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
LGTLSRAQNIVLVATGEGKADAIRGTVEGPVTASCPGSILQMHNNATIIVDEAAASKLEN
HHHHHHCCCEEEEEECCCCCHHEECCCCCCEECCCCHHHHEECCCCEEEEECHHHHHHCC
ADHYRLMEQLKLR
CHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA