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The map label for this gene is shiA [H]

Identifier: 145296634

GI number: 145296634

Start: 2807548

End: 2808879

Strand: Reverse

Name: shiA [H]

Synonym: cgR_2542

Alternate gene names: 145296634

Gene position: 2808879-2807548 (Counterclockwise)

Preceding gene: 145296635

Following gene: 145296632

Centisome position: 84.75

GC content: 55.56

Gene sequence:

>1332_bases
ATGGAAACCGTGAGGACCGCAACCGCCGCTCCTGAAACTGCATCTTTGAAGCTGCGTGAGGCAGAAAGCCCAGCAAAGTC
CCCAAAGAAAGCCGCCTTGGCGTCACTTTTGGGTTCGACTCTGGAGTACTACGACTTTGTCATTTACGGCACCGCCTCCG
CGCTGCTGTTCAATCACCTCTTCTTCCCACAGGGCGACCCAGTGGTCGCGACGATCGGCTCTCTCGCCTCATTCGGTGTT
GCGTACATTGCGCGCCCCATCGGTGGTCTGGTGATGGGACATGTTGGCGATAAGATCAGCCGCAAAACCGCCCTCATGGT
GACGTTGATGATCATGGGTATCGCCTCCATTTCCATCGGACTTCTGCCCACCTACGGACAGATCGGCATTTGGGCGACCG
TGCTGTTGATGATCGCCCGCATCGCACAGGGATTCTCTGCAGGCGCAGAAGCCGCAGGTGCATCCACACTGACCATGGAA
CATTCTCCTGAAGGCAAGCGTGGATTCTTCACCTCATCGGTGATGGCGGGTTGCTCAGTTGGAAACGTCCTGGCTGGCTT
GGTATTTATCCCGTTCTTGATGCTGCCGGAAGAACATCTCATGTCATGGGGCTGGCGCGTACCTTTCCTGCTTTCCGCAC
TGGTTTTAGTTGTCGCATACTTCGTGCGCACCCGACTGGAGGAAGCACCAACTGAGAAGGCCGAAGAGGACGCAGGCGCT
CCGGCTTTGGCTGTGTTGCGCACCCAGGGTATTGATGTCGCACGAGTTTTCCTGATCACCTTCTTCGCCGTCGTTCAGAC
CACTTTCAACGTTTACGCACTGGCATACGCCACCAACGAAGTTGGCATCGATCGTTCCTTCATGGTGATGGTGAACACCA
TCGCGCTGGGGCTTTCCATCGGAACGATTCCTTTGGCCGCGTGGGTCTCTGACCGCATTGGCCGCAAGCCAGTCTTGCTG
TTCGGGGCCATCACCTGTGCAATCACCACTTACTTCTACTTCCAGGCAATCTCTGAAGCTGACCTTGTGCTGATCTTCGC
ACTATGCCTGGTTAACCAAGGTTTGTTCTACTCCTGCTGGAACGGCGTGTGGACCATCTTCTTCCCAGAAATGTTCGCAT
CTTCCGTGCGCTACACCGGCATGGCTATGGGCAACCAGCTCGGTCTGATCATCGTTGGTTTCGCACCAACCATCGCCACC
GCCCTGTACGCATGGAACGGTTGGGAAGCTGTTGCGGGATTCATCATCGGCGCAATCGCACTGTCCGCTGCAGTTATTTT
GACCACCAAGGAAACCGCCTTCACCAAGCTTGAAGATCTAGGGAAGAAATAA

Upstream 100 bases:

>100_bases
CCATTGAATGGGAAGAACTTGGTTGTGTGTTTGTGACACCTATTCTAAAGAACATTAAACGTGATTAAGTTCATGATTCT
TAATGAGAAAGGGTGATCAC

Downstream 100 bases:

>100_bases
TGTCCGACAAGATCTGGAAAGTCGGCATTATCGGTTGCGGTGCAATCAGCCGAAACCACATCGAAGCAGTTCAAGCAATC
CCCGGCGCGGAAGTCAGCGC

Product: hypothetical protein

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
METVRTATAAPETASLKLREAESPAKSPKKAALASLLGSTLEYYDFVIYGTASALLFNHLFFPQGDPVVATIGSLASFGV
AYIARPIGGLVMGHVGDKISRKTALMVTLMIMGIASISIGLLPTYGQIGIWATVLLMIARIAQGFSAGAEAAGASTLTME
HSPEGKRGFFTSSVMAGCSVGNVLAGLVFIPFLMLPEEHLMSWGWRVPFLLSALVLVVAYFVRTRLEEAPTEKAEEDAGA
PALAVLRTQGIDVARVFLITFFAVVQTTFNVYALAYATNEVGIDRSFMVMVNTIALGLSIGTIPLAAWVSDRIGRKPVLL
FGAITCAITTYFYFQAISEADLVLIFALCLVNQGLFYSCWNGVWTIFFPEMFASSVRYTGMAMGNQLGLIIVGFAPTIAT
ALYAWNGWEAVAGFIIGAIALSAAVILTTKETAFTKLEDLGKK

Sequences:

>Translated_443_residues
METVRTATAAPETASLKLREAESPAKSPKKAALASLLGSTLEYYDFVIYGTASALLFNHLFFPQGDPVVATIGSLASFGV
AYIARPIGGLVMGHVGDKISRKTALMVTLMIMGIASISIGLLPTYGQIGIWATVLLMIARIAQGFSAGAEAAGASTLTME
HSPEGKRGFFTSSVMAGCSVGNVLAGLVFIPFLMLPEEHLMSWGWRVPFLLSALVLVVAYFVRTRLEEAPTEKAEEDAGA
PALAVLRTQGIDVARVFLITFFAVVQTTFNVYALAYATNEVGIDRSFMVMVNTIALGLSIGTIPLAAWVSDRIGRKPVLL
FGAITCAITTYFYFQAISEADLVLIFALCLVNQGLFYSCWNGVWTIFFPEMFASSVRYTGMAMGNQLGLIIVGFAPTIAT
ALYAWNGWEAVAGFIIGAIALSAAVILTTKETAFTKLEDLGKK
>Mature_443_residues
METVRTATAAPETASLKLREAESPAKSPKKAALASLLGSTLEYYDFVIYGTASALLFNHLFFPQGDPVVATIGSLASFGV
AYIARPIGGLVMGHVGDKISRKTALMVTLMIMGIASISIGLLPTYGQIGIWATVLLMIARIAQGFSAGAEAAGASTLTME
HSPEGKRGFFTSSVMAGCSVGNVLAGLVFIPFLMLPEEHLMSWGWRVPFLLSALVLVVAYFVRTRLEEAPTEKAEEDAGA
PALAVLRTQGIDVARVFLITFFAVVQTTFNVYALAYATNEVGIDRSFMVMVNTIALGLSIGTIPLAAWVSDRIGRKPVLL
FGAITCAITTYFYFQAISEADLVLIFALCLVNQGLFYSCWNGVWTIFFPEMFASSVRYTGMAMGNQLGLIIVGFAPTIAT
ALYAWNGWEAVAGFIIGAIALSAAVILTTKETAFTKLEDLGKK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=435, Percent_Identity=34.7126436781609, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1789941, Length=438, Percent_Identity=33.7899543378995, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1790550, Length=429, Percent_Identity=31.9347319347319, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788942, Length=367, Percent_Identity=28.8828337874659, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI87082404, Length=419, Percent_Identity=26.491646778043, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI87082231, Length=216, Percent_Identity=26.8518518518519, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47336; Mature: 47336

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METVRTATAAPETASLKLREAESPAKSPKKAALASLLGSTLEYYDFVIYGTASALLFNHL
CCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHH
FFPQGDPVVATIGSLASFGVAYIARPIGGLVMGHVGDKISRKTALMVTLMIMGIASISIG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
LLPTYGQIGIWATVLLMIARIAQGFSAGAEAAGASTLTMEHSPEGKRGFFTSSVMAGCSV
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHCCCH
GNVLAGLVFIPFLMLPEEHLMSWGWRVPFLLSALVLVVAYFVRTRLEEAPTEKAEEDAGA
HHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
PALAVLRTQGIDVARVFLITFFAVVQTTFNVYALAYATNEVGIDRSFMVMVNTIALGLSI
CCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHH
GTIPLAAWVSDRIGRKPVLLFGAITCAITTYFYFQAISEADLVLIFALCLVNQGLFYSCW
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHH
NGVWTIFFPEMFASSVRYTGMAMGNQLGLIIVGFAPTIATALYAWNGWEAVAGFIIGAIA
CCCCEEEEHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHCCCHHHHHHHHHHHHH
LSAAVILTTKETAFTKLEDLGKK
HHHHHEEEECHHHHHHHHHHCCC
>Mature Secondary Structure
METVRTATAAPETASLKLREAESPAKSPKKAALASLLGSTLEYYDFVIYGTASALLFNHL
CCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHH
FFPQGDPVVATIGSLASFGVAYIARPIGGLVMGHVGDKISRKTALMVTLMIMGIASISIG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
LLPTYGQIGIWATVLLMIARIAQGFSAGAEAAGASTLTMEHSPEGKRGFFTSSVMAGCSV
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHCCCH
GNVLAGLVFIPFLMLPEEHLMSWGWRVPFLLSALVLVVAYFVRTRLEEAPTEKAEEDAGA
HHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
PALAVLRTQGIDVARVFLITFFAVVQTTFNVYALAYATNEVGIDRSFMVMVNTIALGLSI
CCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHH
GTIPLAAWVSDRIGRKPVLLFGAITCAITTYFYFQAISEADLVLIFALCLVNQGLFYSCW
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHH
NGVWTIFFPEMFASSVRYTGMAMGNQLGLIIVGFAPTIATALYAWNGWEAVAGFIIGAIA
CCCCEEEEHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHCCCHHHHHHHHHHHHH
LSAAVILTTKETAFTKLEDLGKK
HHHHHEEEECHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]