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The map label for this gene is afr [H]

Identifier: 145296632

GI number: 145296632

Start: 2806484

End: 2807278

Strand: Reverse

Name: afr [H]

Synonym: cgR_2540

Alternate gene names: 145296632

Gene position: 2807278-2806484 (Counterclockwise)

Preceding gene: 145296634

Following gene: 145296631

Centisome position: 84.71

GC content: 56.98

Gene sequence:

>795_bases
GTGCACGTGCTTTGTGAGAAGCCAATCGCCATCGAACTCGATTCTGCGCAACGCATGATCGACGCCTGCGAGTCCGCAGG
CGTCCAACTTGGCGTGCTCTTCCAGCGCCGCTTCTGGCCTGCGGCTCAAAAAATGAAAAAGGAGCTTGTCGACGGCTCCC
TTGGCCAACCCGTCATGGGCCAATGCACGGTGGCGCTTTACCGCGAGCATTCCTATTACACAGCAACCCCGTGGAGGGGA
ACCTGGGCAGCCGACGGCGGTGGAGTGCTCATGACCCAAGCCATCCACTACATCGATCTCATGTGCTGGTTGTTGGGCGA
ACCCGCGGAAGTTTTCGGCTACACCAACTCCTTCAAACACGGCGACAACATCGAAGTCGAAGACAGTGCCGTTGCCACTG
TGCGTTTTGAATCGGGAGCGTTGGCCACAATTTCAGCCACCACCGCCGCCGAACCAGCACTTGGCGCACAAGTGCAGGTG
ATGGGAACAAAGGGTGCCACCATGACGATCTTGGAATTCCCCGAAGGTACTGACGGCAGGCTCATTGTCCGCAGTGAGAA
CGACACCGTGGAAACCACCCCATCCCACCCTGCGGATCTCTATCCCAATGCCGATCTTTCCACCATCAACGGTGCTTTGA
TCCCTTATCACACAGCCCAGATCGCAGACTTTATCGATGCGCTCAATGAAGACCGCCCACCACTGATCACCGGCCGCGAT
GCCACCAGAGCTCTGAAAGTTCTCCTTGGTGTCTATGAATCAGCAACCACCCACCAGCCGGTCTCTTTGATCTAA

Upstream 100 bases:

>100_bases
GTCTGTCGATGAGATCCTCGCCTCCGGGGTGGACATCGTCGCAGTATGCACCCCACATCCAACCCACGAAACAGTGGTCC
TCGCCGCTGCTGCCGCCGGA

Downstream 100 bases:

>100_bases
CGGAAGCTTTTAAAATGTCACACCAAATAGGTCTTGCACCTTTATCCTCCCTGTCCACACCACCTGATCAACTAGTGCAC
CTCGCAGCTGCCGCTGGTTT

Product: hypothetical protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MHVLCEKPIAIELDSAQRMIDACESAGVQLGVLFQRRFWPAAQKMKKELVDGSLGQPVMGQCTVALYREHSYYTATPWRG
TWAADGGGVLMTQAIHYIDLMCWLLGEPAEVFGYTNSFKHGDNIEVEDSAVATVRFESGALATISATTAAEPALGAQVQV
MGTKGATMTILEFPEGTDGRLIVRSENDTVETTPSHPADLYPNADLSTINGALIPYHTAQIADFIDALNEDRPPLITGRD
ATRALKVLLGVYESATTHQPVSLI

Sequences:

>Translated_264_residues
MHVLCEKPIAIELDSAQRMIDACESAGVQLGVLFQRRFWPAAQKMKKELVDGSLGQPVMGQCTVALYREHSYYTATPWRG
TWAADGGGVLMTQAIHYIDLMCWLLGEPAEVFGYTNSFKHGDNIEVEDSAVATVRFESGALATISATTAAEPALGAQVQV
MGTKGATMTILEFPEGTDGRLIVRSENDTVETTPSHPADLYPNADLSTINGALIPYHTAQIADFIDALNEDRPPLITGRD
ATRALKVLLGVYESATTHQPVSLI
>Mature_264_residues
MHVLCEKPIAIELDSAQRMIDACESAGVQLGVLFQRRFWPAAQKMKKELVDGSLGQPVMGQCTVALYREHSYYTATPWRG
TWAADGGGVLMTQAIHYIDLMCWLLGEPAEVFGYTNSFKHGDNIEVEDSAVATVRFESGALATISATTAAEPALGAQVQV
MGTKGATMTILEFPEGTDGRLIVRSENDTVETTPSHPADLYPNADLSTINGALIPYHTAQIADFIDALNEDRPPLITGRD
ATRALKVLLGVYESATTHQPVSLI

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 28568; Mature: 28568

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVLCEKPIAIELDSAQRMIDACESAGVQLGVLFQRRFWPAAQKMKKELVDGSLGQPVMG
CCEEECCCCEEEECHHHHHHHHHHHCCCEEEEEHHHHCCHHHHHHHHHHHCCCCCCCCHH
QCTVALYREHSYYTATPWRGTWAADGGGVLMTQAIHYIDLMCWLLGEPAEVFGYTNSFKH
HEEEEEEECCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCC
GDNIEVEDSAVATVRFESGALATISATTAAEPALGAQVQVMGTKGATMTILEFPEGTDGR
CCCCEECCCEEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCE
LIVRSENDTVETTPSHPADLYPNADLSTINGALIPYHTAQIADFIDALNEDRPPLITGRD
EEEECCCCCEEECCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCH
ATRALKVLLGVYESATTHQPVSLI
HHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MHVLCEKPIAIELDSAQRMIDACESAGVQLGVLFQRRFWPAAQKMKKELVDGSLGQPVMG
CCEEECCCCEEEECHHHHHHHHHHHCCCEEEEEHHHHCCHHHHHHHHHHHCCCCCCCCHH
QCTVALYREHSYYTATPWRGTWAADGGGVLMTQAIHYIDLMCWLLGEPAEVFGYTNSFKH
HEEEEEEECCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCC
GDNIEVEDSAVATVRFESGALATISATTAAEPALGAQVQVMGTKGATMTILEFPEGTDGR
CCCCEECCCEEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCE
LIVRSENDTVETTPSHPADLYPNADLSTINGALIPYHTAQIADFIDALNEDRPPLITGRD
EEEECCCCCEEECCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCH
ATRALKVLLGVYESATTHQPVSLI
HHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA