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The map label for this gene is otsB [H]

Identifier: 145296625

GI number: 145296625

Start: 2799026

End: 2799796

Strand: Direct

Name: otsB [H]

Synonym: cgR_2533

Alternate gene names: 145296625

Gene position: 2799026-2799796 (Clockwise)

Preceding gene: 145296624

Following gene: 145296627

Centisome position: 84.46

GC content: 50.97

Gene sequence:

>771_bases
ATGACTTTGACTATTGAGGAAATCGCCAAGACCAAAAAGCTTTTGGTTGTGTCCGATTTTGATGGAACCATCGCAGGATT
TAGCAAGGACGCTTACAACGTTCCTATCAACCAGAAATCCCTCAAGGCGGTTAAAGACCTCTCCCAACAAGCAGACACTG
ATGTTGTCATTTTGTCGGGACGCCACCTGGAGGGATTGAAAACGGTTCTTGATCTTGGTCAGTACGACATCACCATGGTG
GGTTCACACGGTTCTGAGGATTCCTCCCGCCCGCGTACCCTCACTCCTGAAGAGGTAGCTCGCCTCGCCAAGATTGAAGC
AGATCTGGAAAAGATCGTCGACGGCATCGAAGGCGCATTCGTGGAGATCAAGCCTTTCCACCGCGTGCTGCACTTCATCC
GTGTTTCCGACAAGAACAAAGTCCAAGAAATCCTCGACCAAGCAGCACACGTAGACTCTTCCGGCTTGAAGGTAACCAAC
GGCAAGAGCATCATCGAATACTCCATCAGCTCCACCACCAAGGGAACCTGGCTTAAGGAATACGTTGACCGCACAGAGCC
CACTGGTGTGATTTTCCTCGGCGATGACACCACCGATGAGCACGGTTTCAAAGCTTTAGAAAACGATGATCGTGCCCTAA
CCGTCAAGGTTGGCGAAGGAGACACTGCAGCCAAAACCCGCGTCGACGATGTGGATAATGTGGGAATTTTCCTAGAGAAA
CTCGCCTACCACCGCATGCAGTATGCGGAAAGCGTGCGATTGGGGATTTAA

Upstream 100 bases:

>100_bases
TCGGCCAGCAATCCGCTTGGTGTCCTGGATCGCGCCGACATCTTAAGGCGCCAGGGCTTTAAAGTGCCAGGGGTTCTGTG
GGATCCGTACACTGGTTCCC

Downstream 100 bases:

>100_bases
GAGAGCCTAAACGCACGAAAAGTGCCACAAGCCTAGATTGGCGCAACCGTGGAACCTGGGTGGAAGAAAGTTTCCAATTC
CACTTCTGGTTCCACGTATT

Product: hypothetical protein

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTLTIEEIAKTKKLLVVSDFDGTIAGFSKDAYNVPINQKSLKAVKDLSQQADTDVVILSGRHLEGLKTVLDLGQYDITMV
GSHGSEDSSRPRTLTPEEVARLAKIEADLEKIVDGIEGAFVEIKPFHRVLHFIRVSDKNKVQEILDQAAHVDSSGLKVTN
GKSIIEYSISSTTKGTWLKEYVDRTEPTGVIFLGDDTTDEHGFKALENDDRALTVKVGEGDTAAKTRVDDVDNVGIFLEK
LAYHRMQYAESVRLGI

Sequences:

>Translated_256_residues
MTLTIEEIAKTKKLLVVSDFDGTIAGFSKDAYNVPINQKSLKAVKDLSQQADTDVVILSGRHLEGLKTVLDLGQYDITMV
GSHGSEDSSRPRTLTPEEVARLAKIEADLEKIVDGIEGAFVEIKPFHRVLHFIRVSDKNKVQEILDQAAHVDSSGLKVTN
GKSIIEYSISSTTKGTWLKEYVDRTEPTGVIFLGDDTTDEHGFKALENDDRALTVKVGEGDTAAKTRVDDVDNVGIFLEK
LAYHRMQYAESVRLGI
>Mature_255_residues
TLTIEEIAKTKKLLVVSDFDGTIAGFSKDAYNVPINQKSLKAVKDLSQQADTDVVILSGRHLEGLKTVLDLGQYDITMVG
SHGSEDSSRPRTLTPEEVARLAKIEADLEKIVDGIEGAFVEIKPFHRVLHFIRVSDKNKVQEILDQAAHVDSSGLKVTNG
KSIIEYSISSTTKGTWLKEYVDRTEPTGVIFLGDDTTDEHGFKALENDDRALTVKVGEGDTAAKTRVDDVDNVGIFLEKL
AYHRMQYAESVRLGI

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=241, Percent_Identity=26.5560165975104, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 28220; Mature: 28089

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTIEEIAKTKKLLVVSDFDGTIAGFSKDAYNVPINQKSLKAVKDLSQQADTDVVILSG
CEEEHHHHHCCCEEEEEECCCCCEECCCCCCEECCCCHHHHHHHHHHHHHCCCCEEEEEC
RHLEGLKTVLDLGQYDITMVGSHGSEDSSRPRTLTPEEVARLAKIEADLEKIVDGIEGAF
CCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
VEIKPFHRVLHFIRVSDKNKVQEILDQAAHVDSSGLKVTNGKSIIEYSISSTTKGTWLKE
EEEHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCEEEEEECCCCCCCHHHHH
YVDRTEPTGVIFLGDDTTDEHGFKALENDDRALTVKVGEGDTAAKTRVDDVDNVGIFLEK
HHHCCCCCEEEEECCCCCCCCCCHHHCCCCCEEEEEECCCCCHHHCCCCCCCHHHHHHHH
LAYHRMQYAESVRLGI
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLTIEEIAKTKKLLVVSDFDGTIAGFSKDAYNVPINQKSLKAVKDLSQQADTDVVILSG
EEEHHHHHCCCEEEEEECCCCCEECCCCCCEECCCCHHHHHHHHHHHHHCCCCEEEEEC
RHLEGLKTVLDLGQYDITMVGSHGSEDSSRPRTLTPEEVARLAKIEADLEKIVDGIEGAF
CCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
VEIKPFHRVLHFIRVSDKNKVQEILDQAAHVDSSGLKVTNGKSIIEYSISSTTKGTWLKE
EEEHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCEEEEEECCCCCCCHHHHH
YVDRTEPTGVIFLGDDTTDEHGFKALENDDRALTVKVGEGDTAAKTRVDDVDNVGIFLEK
HHHCCCCCEEEEECCCCCCCCCCHHHCCCCCEEEEEECCCCCHHHCCCCCCCHHHHHHHH
LAYHRMQYAESVRLGI
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]