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The map label for this gene is dus [H]

Identifier: 145296565

GI number: 145296565

Start: 2730422

End: 2731513

Strand: Direct

Name: dus [H]

Synonym: cgR_2473

Alternate gene names: 145296565

Gene position: 2730422-2731513 (Clockwise)

Preceding gene: 145296558

Following gene: 145296566

Centisome position: 82.39

GC content: 56.5

Gene sequence:

>1092_bases
ATGGCTGGTGTAACCAACGTTGCTTTCCGCACGCTGTGCCGTGAACAGGAAATGCAACGCACGGGAACAATCTCGGGGCT
GTACGTCTGTGAAATGGTGACTGCACGTGCTCTTGTTGAGCGCAATGAGAAAACCATGCACATGACCACCTTCGCGCCGG
ATGAAAATCCCCGAAGCTTGCAGCTGTACACGGTTGACCCGAAGTATACCTACGAAGCGGCGAAGATGATCGTTGATGAA
AACTTGGCGGATCATATTGATATGAACTTTGGCTGCCCGGTTCCAAAGGTCACGCGCCGGGGTGGCGGTTCTGCAATTCC
TTACAAGCGCCGTTTGTTTGAAAACATCGTTTCCGCGGCTGTGAAGGCTACGGAAGGCACGGACATTCCGGTGACGGTGA
AATTCCGCGTTGGTATTGATGATGAGCACCATACTCACTTGGATGCTGGACGCATTGCTGTCGACGCCGGCGCGAAGTCC
GTAGCGCTTCACGCCCGCACCGCAGCGCAGCGTTATTCCGGTGAGGCTGACTGGAATGAGATCGCGCGCCTGAAGGAGCA
TTTGGCAGATACCGGCATCCCAGTTTTGGGCAATGGCGATATTTTCGCGGCATCCGATGCAACGCGCATGATGGAGCAAA
CTGGCTGCGATGGCGTCGTGGTTGGGCGTGGTTGCCTGGGCAGGCCTTGGCTCTTTGCTGAGCTGTCTGCTGCTGTTCGT
GGAGAAGAAATCCCAGAGGAGCCTACCTTCGGCGAGGTTACTCAGATCATCCTTCGCCATGCTGAGCTTTTGATGCAGCA
TGATGGCGAAACCAAGGGGCTGCGCGATCTGCGCAAGCACATGGGTTGGTACCTGCGCGGTTTCCCTGTTGGCGGCGAAT
TCCGCTCCAATCTGGCCAAGGTTTCCACCTATGTGGAGCTTGAGGATCTACTAGCACCATGGGCTGACTCCACCGCCAAG
GCAGAGGACGCGGAAGGTGCACGAGGTCGGCAGGGTGCTCCAGCAAAGGTGGCACTTCCAGACGGCTGGTTGGACGATCC
TGAGGATGCCACTGTTCCTAAAGGCGCAGAAATGGAAAACTCCGGAGGGTAG

Upstream 100 bases:

>100_bases
GCTTCAAATATTTTGCCCAACCTGATTCACGGGGGACAATAGTTAGGTGACTTTAAAAATCGGCCCCTTTGACCTTGCCT
CCCCTGTGGTTCTAGCCCCC

Downstream 100 bases:

>100_bases
TTAATTTAATACTTACCCCCGCCAATAGTTGGCTATCCCCCATTATTAGGTGATTCAATCTTAAAGGGGTGTTTTAGGTC
TGCAGAAATACCATTATCGC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MAGVTNVAFRTLCREQEMQRTGTISGLYVCEMVTARALVERNEKTMHMTTFAPDENPRSLQLYTVDPKYTYEAAKMIVDE
NLADHIDMNFGCPVPKVTRRGGGSAIPYKRRLFENIVSAAVKATEGTDIPVTVKFRVGIDDEHHTHLDAGRIAVDAGAKS
VALHARTAAQRYSGEADWNEIARLKEHLADTGIPVLGNGDIFAASDATRMMEQTGCDGVVVGRGCLGRPWLFAELSAAVR
GEEIPEEPTFGEVTQIILRHAELLMQHDGETKGLRDLRKHMGWYLRGFPVGGEFRSNLAKVSTYVELEDLLAPWADSTAK
AEDAEGARGRQGAPAKVALPDGWLDDPEDATVPKGAEMENSGG

Sequences:

>Translated_363_residues
MAGVTNVAFRTLCREQEMQRTGTISGLYVCEMVTARALVERNEKTMHMTTFAPDENPRSLQLYTVDPKYTYEAAKMIVDE
NLADHIDMNFGCPVPKVTRRGGGSAIPYKRRLFENIVSAAVKATEGTDIPVTVKFRVGIDDEHHTHLDAGRIAVDAGAKS
VALHARTAAQRYSGEADWNEIARLKEHLADTGIPVLGNGDIFAASDATRMMEQTGCDGVVVGRGCLGRPWLFAELSAAVR
GEEIPEEPTFGEVTQIILRHAELLMQHDGETKGLRDLRKHMGWYLRGFPVGGEFRSNLAKVSTYVELEDLLAPWADSTAK
AEDAEGARGRQGAPAKVALPDGWLDDPEDATVPKGAEMENSGG
>Mature_362_residues
AGVTNVAFRTLCREQEMQRTGTISGLYVCEMVTARALVERNEKTMHMTTFAPDENPRSLQLYTVDPKYTYEAAKMIVDEN
LADHIDMNFGCPVPKVTRRGGGSAIPYKRRLFENIVSAAVKATEGTDIPVTVKFRVGIDDEHHTHLDAGRIAVDAGAKSV
ALHARTAAQRYSGEADWNEIARLKEHLADTGIPVLGNGDIFAASDATRMMEQTGCDGVVVGRGCLGRPWLFAELSAAVRG
EEIPEEPTFGEVTQIILRHAELLMQHDGETKGLRDLRKHMGWYLRGFPVGGEFRSNLAKVSTYVELEDLLAPWADSTAKA
EDAEGARGRQGAPAKVALPDGWLDDPEDATVPKGAEMENSGG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=294, Percent_Identity=29.5918367346939, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI239788462, Length=294, Percent_Identity=29.2517006802721, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI40807366, Length=369, Percent_Identity=26.8292682926829, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI31742496, Length=233, Percent_Identity=28.3261802575107, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1789660, Length=320, Percent_Identity=32.8125, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1788462, Length=289, Percent_Identity=28.3737024221453, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI145693211, Length=285, Percent_Identity=24.5614035087719, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17543114, Length=242, Percent_Identity=28.5123966942149, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=233, Percent_Identity=29.1845493562232, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17507177, Length=242, Percent_Identity=30.1652892561983, Blast_Score=78, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323560, Length=269, Percent_Identity=27.1375464684015, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=237, Percent_Identity=29.1139240506329, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323433, Length=227, Percent_Identity=26.431718061674, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24585320, Length=286, Percent_Identity=28.3216783216783, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24580595, Length=241, Percent_Identity=29.045643153527, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19920448, Length=241, Percent_Identity=29.045643153527, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19921524, Length=234, Percent_Identity=28.2051282051282, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 39624; Mature: 39493

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGVTNVAFRTLCREQEMQRTGTISGLYVCEMVTARALVERNEKTMHMTTFAPDENPRSL
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEE
QLYTVDPKYTYEAAKMIVDENLADHIDMNFGCPVPKVTRRGGGSAIPYKRRLFENIVSAA
EEEECCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHH
VKATEGTDIPVTVKFRVGIDDEHHTHLDAGRIAVDAGAKSVALHARTAAQRYSGEADWNE
HHHCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEECCCCCEEHHHHHHHHHCCCCCCHHH
IARLKEHLADTGIPVLGNGDIFAASDATRMMEQTGCDGVVVGRGCLGRPWLFAELSAAVR
HHHHHHHHHHCCCCEECCCCEEECCHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHC
GEEIPEEPTFGEVTQIILRHAELLMQHDGETKGLRDLRKHMGWYLRGFPVGGEFRSNLAK
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHH
VSTYVELEDLLAPWADSTAKAEDAEGARGRQGAPAKVALPDGWLDDPEDATVPKGAEMEN
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
SGG
CCC
>Mature Secondary Structure 
AGVTNVAFRTLCREQEMQRTGTISGLYVCEMVTARALVERNEKTMHMTTFAPDENPRSL
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEE
QLYTVDPKYTYEAAKMIVDENLADHIDMNFGCPVPKVTRRGGGSAIPYKRRLFENIVSAA
EEEECCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHH
VKATEGTDIPVTVKFRVGIDDEHHTHLDAGRIAVDAGAKSVALHARTAAQRYSGEADWNE
HHHCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEECCCCCEEHHHHHHHHHCCCCCCHHH
IARLKEHLADTGIPVLGNGDIFAASDATRMMEQTGCDGVVVGRGCLGRPWLFAELSAAVR
HHHHHHHHHHCCCCEECCCCEEECCHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHC
GEEIPEEPTFGEVTQIILRHAELLMQHDGETKGLRDLRKHMGWYLRGFPVGGEFRSNLAK
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHH
VSTYVELEDLLAPWADSTAKAEDAEGARGRQGAPAKVALPDGWLDDPEDATVPKGAEMEN
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
SGG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]