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The map label for this gene is metB [H]

Identifier: 145296434

GI number: 145296434

Start: 2584309

End: 2585469

Strand: Reverse

Name: metB [H]

Synonym: cgR_2348

Alternate gene names: 145296434

Gene position: 2585469-2584309 (Counterclockwise)

Preceding gene: 145296435

Following gene: 145296432

Centisome position: 78.01

GC content: 56.85

Gene sequence:

>1161_bases
TTGTCTTTTGACCCACACACCCAGGGTTTCTCCACCGCATCCATTCACGCTGGGTATGAGCCAGACGACTACTACGGTTC
GATCAACATGCCGATCTACGCCTCCACCACCTTCGCACAGAACGCTCCCAACGATCTGCGCCGCGGCTTTGAATACACTC
GCGTGGGCAACCCCACTATCGTGTCCTTGGAAAAGGCCGTCGCAGCGTTGGAAGGTGCAGAGTATGGTCGTGCATTCTCC
TCCGGCATGGCTGCCACTGACATCCTCTTCCGCGTTCTACTCAAGCCAGGTGACCATATTGTGCTCGGAAACGACGCATA
CGGCGGAACCTTCCGCTTGATCGATACCGTGTTCACCGCGTGGGGAATCACCTACACCGTGGTCGACACCTCTGTGGTTG
ACGAAGTCAAGAATGCCATCCAAGACAATACCAAGCTGATCTGGGTGGAAACCCCCACCAACCCAGCACTGGGGATCACC
GATATCGACGCTGTAGCTAAGCTCACTGAAGGCACCAACGCGAAGCTCGTCGTGGACAACACCTTCGCCTCCCCATACCT
GCAGCAGCCACTGGCTCTCGGCGCACATGCTGTCCTGCACTCCACCACCAAGTACATCGGTGGCCACTCTGACGTCGTAG
GTGGACTCGTAGTCACCAACGATCCGGCTCTCGACGAAGAACTGCTGTTCATGCAGGGCGGCGTTGGCCCAATTCCATCT
GTCTTCGATGCTTACCTCACTGCTCGTGGCTTAAAGACCCTGGCTGTGCGCATGGATCGCCACTGTGACAACGCAGAAAA
GGTGGCAGAATTCTTAGATTCCCGTGACGAGGTTTCCGTAGTTCTCTATCCAGGACTGAAGAACCACCCAGGTCACGAGG
TTGCAGCGAAGCAGATGAAGCGCTTCGGCGGCATGATCTCCGTCCGTTTCGCAGGCGGCGAAGAAGCAGCTAAGAAGTTC
TGTACCTCTACCAAGCTGATCTGTCTGGCCGAGTCCCTCGGTGGCGTGGAATCCCTCCTGGAGCACCCAGCAACCATGAC
CCACCAGTCAGCTGCCGGCTCTCAGCTCGAGGTTCCCCGCGACCTCGTGCGCATCTCCATTGGTATTGAAGACATTGAAG
ACCTGCTCGCAGATGTCGAGCAGGCCCTCAATAACCTTTAG

Upstream 100 bases:

>100_bases
AAGGTGATAGATTTGGGCAAAAATGGACAGCTTGGTCTATCATTGCGGAAAAGACCTGTTTATACAGGTGTCGGAAATGA
GTATCAGGAGCTGATCTACC

Downstream 100 bases:

>100_bases
AAACTATTTGGCGGCAAGCAGCTTTTCAATATAAGCAATGCGAGCCTCCACCATGTAGCCGAAGAGTTCGTCAGAAGTTG
AGACGGACTCTTCGACTGCT

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSFDPHTQGFSTASIHAGYEPDDYYGSINMPIYASTTFAQNAPNDLRRGFEYTRVGNPTIVSLEKAVAALEGAEYGRAFS
SGMAATDILFRVLLKPGDHIVLGNDAYGGTFRLIDTVFTAWGITYTVVDTSVVDEVKNAIQDNTKLIWVETPTNPALGIT
DIDAVAKLTEGTNAKLVVDNTFASPYLQQPLALGAHAVLHSTTKYIGGHSDVVGGLVVTNDPALDEELLFMQGGVGPIPS
VFDAYLTARGLKTLAVRMDRHCDNAEKVAEFLDSRDEVSVVLYPGLKNHPGHEVAAKQMKRFGGMISVRFAGGEEAAKKF
CTSTKLICLAESLGGVESLLEHPATMTHQSAAGSQLEVPRDLVRISIGIEDIEDLLADVEQALNNL

Sequences:

>Translated_386_residues
MSFDPHTQGFSTASIHAGYEPDDYYGSINMPIYASTTFAQNAPNDLRRGFEYTRVGNPTIVSLEKAVAALEGAEYGRAFS
SGMAATDILFRVLLKPGDHIVLGNDAYGGTFRLIDTVFTAWGITYTVVDTSVVDEVKNAIQDNTKLIWVETPTNPALGIT
DIDAVAKLTEGTNAKLVVDNTFASPYLQQPLALGAHAVLHSTTKYIGGHSDVVGGLVVTNDPALDEELLFMQGGVGPIPS
VFDAYLTARGLKTLAVRMDRHCDNAEKVAEFLDSRDEVSVVLYPGLKNHPGHEVAAKQMKRFGGMISVRFAGGEEAAKKF
CTSTKLICLAESLGGVESLLEHPATMTHQSAAGSQLEVPRDLVRISIGIEDIEDLLADVEQALNNL
>Mature_385_residues
SFDPHTQGFSTASIHAGYEPDDYYGSINMPIYASTTFAQNAPNDLRRGFEYTRVGNPTIVSLEKAVAALEGAEYGRAFSS
GMAATDILFRVLLKPGDHIVLGNDAYGGTFRLIDTVFTAWGITYTVVDTSVVDEVKNAIQDNTKLIWVETPTNPALGITD
IDAVAKLTEGTNAKLVVDNTFASPYLQQPLALGAHAVLHSTTKYIGGHSDVVGGLVVTNDPALDEELLFMQGGVGPIPSV
FDAYLTARGLKTLAVRMDRHCDNAEKVAEFLDSRDEVSVVLYPGLKNHPGHEVAAKQMKRFGGMISVRFAGGEEAAKKFC
TSTKLICLAESLGGVESLLEHPATMTHQSAAGSQLEVPRDLVRISIGIEDIEDLLADVEQALNNL

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=387, Percent_Identity=47.8036175710594, Blast_Score=320, Evalue=1e-87,
Organism=Homo sapiens, GI299473759, Length=387, Percent_Identity=43.6692506459948, Blast_Score=275, Evalue=4e-74,
Organism=Homo sapiens, GI25453487, Length=386, Percent_Identity=42.2279792746114, Blast_Score=254, Evalue=9e-68,
Organism=Escherichia coli, GI1790375, Length=385, Percent_Identity=40.7792207792208, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI1789383, Length=338, Percent_Identity=29.585798816568, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17538055, Length=391, Percent_Identity=46.5473145780051, Blast_Score=320, Evalue=6e-88,
Organism=Caenorhabditis elegans, GI17560056, Length=390, Percent_Identity=45.3846153846154, Blast_Score=317, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI71980708, Length=369, Percent_Identity=39.0243902439024, Blast_Score=248, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6319307, Length=384, Percent_Identity=51.0416666666667, Blast_Score=345, Evalue=9e-96,
Organism=Saccharomyces cerevisiae, GI6321254, Length=403, Percent_Identity=32.0099255583127, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6323333, Length=431, Percent_Identity=31.322505800464, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6321170, Length=331, Percent_Identity=30.2114803625378, Blast_Score=153, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6321904, Length=394, Percent_Identity=28.4263959390863, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19922554, Length=389, Percent_Identity=46.0154241645244, Blast_Score=338, Evalue=3e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41491; Mature: 41360

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFDPHTQGFSTASIHAGYEPDDYYGSINMPIYASTTFAQNAPNDLRRGFEYTRVGNPTI
CCCCCCCCCCCCEEEECCCCCCCCEEEECCCEEEECCCCCCCCHHHHCCCCEEECCCCCE
VSLEKAVAALEGAEYGRAFSSGMAATDILFRVLLKPGDHIVLGNDAYGGTFRLIDTVFTA
EHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHH
WGITYTVVDTSVVDEVKNAIQDNTKLIWVETPTNPALGITDIDAVAKLTEGTNAKLVVDN
HCCEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCCEEEEEC
TFASPYLQQPLALGAHAVLHSTTKYIGGHSDVVGGLVVTNDPALDEELLFMQGGVGPIPS
CCCCHHHHCHHHHHHHHHHHHHHHHCCCCCHHHCCEEEECCCCCCHHHHHCCCCCCCCHH
VFDAYLTARGLKTLAVRMDRHCDNAEKVAEFLDSRDEVSVVLYPGLKNHPGHEVAAKQMK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHH
RFGGMISVRFAGGEEAAKKFCTSTKLICLAESLGGVESLLEHPATMTHQSAAGSQLEVPR
HHCCEEEEEECCCHHHHHHHHCCCHHEEEHHHHCHHHHHHHCCCHHHHCCCCCCCCCCCH
DLVRISIGIEDIEDLLADVEQALNNL
HHHEEECCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SFDPHTQGFSTASIHAGYEPDDYYGSINMPIYASTTFAQNAPNDLRRGFEYTRVGNPTI
CCCCCCCCCCCEEEECCCCCCCCEEEECCCEEEECCCCCCCCHHHHCCCCEEECCCCCE
VSLEKAVAALEGAEYGRAFSSGMAATDILFRVLLKPGDHIVLGNDAYGGTFRLIDTVFTA
EHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHH
WGITYTVVDTSVVDEVKNAIQDNTKLIWVETPTNPALGITDIDAVAKLTEGTNAKLVVDN
HCCEEEEECHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCCEEEEEC
TFASPYLQQPLALGAHAVLHSTTKYIGGHSDVVGGLVVTNDPALDEELLFMQGGVGPIPS
CCCCHHHHCHHHHHHHHHHHHHHHHCCCCCHHHCCEEEECCCCCCHHHHHCCCCCCCCHH
VFDAYLTARGLKTLAVRMDRHCDNAEKVAEFLDSRDEVSVVLYPGLKNHPGHEVAAKQMK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHH
RFGGMISVRFAGGEEAAKKFCTSTKLICLAESLGGVESLLEHPATMTHQSAAGSQLEVPR
HHCCEEEEEECCCHHHHHHHHCCCHHEEEHHHHCHHHHHHHCCCHHHHCCCCCCCCCCCH
DLVRISIGIEDIEDLLADVEQALNNL
HHHEEECCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]