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The map label for this gene is kgtP [H]

Identifier: 145296412

GI number: 145296412

Start: 2558662

End: 2560053

Strand: Direct

Name: kgtP [H]

Synonym: cgR_2326

Alternate gene names: 145296412

Gene position: 2558662-2560053 (Clockwise)

Preceding gene: 145296408

Following gene: 145296413

Centisome position: 77.2

GC content: 53.81

Gene sequence:

>1392_bases
ATGACAACCTCTACTGCTGACATCACCCCCGCACTCTCATCATCGCCTGCTTCTAATGCCACTCGCCGCCAATCCCTGCT
CGCCACCGGAGTGGGTAACGTCCTGGAATGGTTTGACTGGGCGGTCTATGGAGTGTTCTCCACTTACATCGCAATGGCCA
TGTTTAATCAGGACAACCCCGTCTCCGCATTGTTGTCCACTCTCGCGGTATTTGCTGTCGGTTTCGTCATGCGCCCCCTT
GGTGGCTTTGTGTTCGGAAGGATTGCTGACCGCAAGGGGCGTAAATGGGTTCTTCTGGTCACCATGCTCATGATGGCCAG
TGGTTCATTACTCATCGGCATCATCCCCAGCTATGAAACTATCGGCGGGTTTGCATCTTTCCTGCTCCTGCTGGCTAGAT
TGATCCAGGGATTCGCCCATGGTGGGGAAGCAACCGCCTCGAATGTTTACCTGCCTGAAATTGCTCCTCGCCACCGCCGT
GCTCTCTATGGATCTACCATCGGCTTTGCCATGGGATTGGGCACGATGATCGCTATCTTGTTCGGTTCTGTTTTAACTAA
TATCTTTGACTCAGCCGTCATGAATGAGTGGGGCTGGCGTATCCCGTTCATCTTTGGTGGGCTGCTAGCAGTGGTCGTCC
TGTGGCTCCGCCGTAACATGATGGAGTCCGAGGTTCATGAGGCCCATGTCGAAATCGCCCAGATGGCTAATGACGTGGAA
GAAAATCCATCTCTCGCTCAGGTCAAAACTGCAGCAACTGACGAGGCCAGTAAAGTCAATGTTGTAGTTGAATGGCCTAA
TCGGAAGATTTTTGTCAAAGCAGTCGAAGTCTTCTTTTACATGGCTGGCACCACACTGCCGTATTACATCTGGTCCTCTT
TCGCGGCGACCTATGCCATCACTCAAAAAGATATGGATCCAGCGGGTGCCTTTACTGCATCCTTAGGAGCCATGGCAGTC
AACTTGGCACTCGTGCCAATCATGGGTCTGATTGCGGACAAGATCGGACGACGCATTCCAGTCCTAGTTTACGCGCTTGC
TACCGCTGCATTGACTGTTCCCGTCTTCGCGATGATCAACGACAATCCCTGGACCCTATTTATCGCCCAGGCTCTTATGA
TGGGACTTAGCGCCTGTATCGGTGGAACACAGCCAGCCATGCTTGCTGAGCAAATCCCCACTCGCTACCGCACCCTCATC
ATGGGAACCGCCATGCCCCTGGCCGTCGCCCTCTTCGGTGGCACTGCCCCCTACATCAACACCTGGCTGGCTTCCATCGA
TATGTTCTGGCTCTTCGACTCCTACATCATCGCCATGACGCTGGGCACCGCTATTGTCGTGGGCTTCCGCTGGAAGGAAA
CCAAGGGAATCCACCTCGTCGATGTCCGCTAA

Upstream 100 bases:

>100_bases
TAAAAAGTCCATTAAATGAACTTTTAATTGTCGTTATCATCCTCGTGTTGTCGGGTAAATATATGCCCGTCCCCCCCCCT
CCGCTGAACAAAGGAACAGC

Downstream 100 bases:

>100_bases
CCGCACACCTAAAAAGCAGGAGTATTTATGCTTACCCAGGATCTAATGATTCCGCGCACCACCACTATTAACGATGTAGA
AATCACATATTACGACTCTG

Product: hypothetical protein

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MTTSTADITPALSSSPASNATRRQSLLATGVGNVLEWFDWAVYGVFSTYIAMAMFNQDNPVSALLSTLAVFAVGFVMRPL
GGFVFGRIADRKGRKWVLLVTMLMMASGSLLIGIIPSYETIGGFASFLLLLARLIQGFAHGGEATASNVYLPEIAPRHRR
ALYGSTIGFAMGLGTMIAILFGSVLTNIFDSAVMNEWGWRIPFIFGGLLAVVVLWLRRNMMESEVHEAHVEIAQMANDVE
ENPSLAQVKTAATDEASKVNVVVEWPNRKIFVKAVEVFFYMAGTTLPYYIWSSFAATYAITQKDMDPAGAFTASLGAMAV
NLALVPIMGLIADKIGRRIPVLVYALATAALTVPVFAMINDNPWTLFIAQALMMGLSACIGGTQPAMLAEQIPTRYRTLI
MGTAMPLAVALFGGTAPYINTWLASIDMFWLFDSYIIAMTLGTAIVVGFRWKETKGIHLVDVR

Sequences:

>Translated_463_residues
MTTSTADITPALSSSPASNATRRQSLLATGVGNVLEWFDWAVYGVFSTYIAMAMFNQDNPVSALLSTLAVFAVGFVMRPL
GGFVFGRIADRKGRKWVLLVTMLMMASGSLLIGIIPSYETIGGFASFLLLLARLIQGFAHGGEATASNVYLPEIAPRHRR
ALYGSTIGFAMGLGTMIAILFGSVLTNIFDSAVMNEWGWRIPFIFGGLLAVVVLWLRRNMMESEVHEAHVEIAQMANDVE
ENPSLAQVKTAATDEASKVNVVVEWPNRKIFVKAVEVFFYMAGTTLPYYIWSSFAATYAITQKDMDPAGAFTASLGAMAV
NLALVPIMGLIADKIGRRIPVLVYALATAALTVPVFAMINDNPWTLFIAQALMMGLSACIGGTQPAMLAEQIPTRYRTLI
MGTAMPLAVALFGGTAPYINTWLASIDMFWLFDSYIIAMTLGTAIVVGFRWKETKGIHLVDVR
>Mature_462_residues
TTSTADITPALSSSPASNATRRQSLLATGVGNVLEWFDWAVYGVFSTYIAMAMFNQDNPVSALLSTLAVFAVGFVMRPLG
GFVFGRIADRKGRKWVLLVTMLMMASGSLLIGIIPSYETIGGFASFLLLLARLIQGFAHGGEATASNVYLPEIAPRHRRA
LYGSTIGFAMGLGTMIAILFGSVLTNIFDSAVMNEWGWRIPFIFGGLLAVVVLWLRRNMMESEVHEAHVEIAQMANDVEE
NPSLAQVKTAATDEASKVNVVVEWPNRKIFVKAVEVFFYMAGTTLPYYIWSSFAATYAITQKDMDPAGAFTASLGAMAVN
LALVPIMGLIADKIGRRIPVLVYALATAALTVPVFAMINDNPWTLFIAQALMMGLSACIGGTQPAMLAEQIPTRYRTLIM
GTAMPLAVALFGGTAPYINTWLASIDMFWLFDSYIIAMTLGTAIVVGFRWKETKGIHLVDVR

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=461, Percent_Identity=34.70715835141, Blast_Score=224, Evalue=7e-60,
Organism=Escherichia coli, GI1790550, Length=446, Percent_Identity=29.1479820627803, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1788292, Length=402, Percent_Identity=29.3532338308458, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1789941, Length=420, Percent_Identity=30.952380952381, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87082231, Length=484, Percent_Identity=24.3801652892562, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI87082404, Length=419, Percent_Identity=22.4343675417661, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 50282; Mature: 50151

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSTADITPALSSSPASNATRRQSLLATGVGNVLEWFDWAVYGVFSTYIAMAMFNQDNP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
VSALLSTLAVFAVGFVMRPLGGFVFGRIADRKGRKWVLLVTMLMMASGSLLIGIIPSYET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCHHH
IGGFASFLLLLARLIQGFAHGGEATASNVYLPEIAPRHRRALYGSTIGFAMGLGTMIAIL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHH
FGSVLTNIFDSAVMNEWGWRIPFIFGGLLAVVVLWLRRNMMESEVHEAHVEIAQMANDVE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENPSLAQVKTAATDEASKVNVVVEWPNRKIFVKAVEVFFYMAGTTLPYYIWSSFAATYAI
CCCCHHHEEHHCCCCCCEEEEEEECCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHEE
TQKDMDPAGAFTASLGAMAVNLALVPIMGLIADKIGRRIPVLVYALATAALTVPVFAMIN
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEEC
DNPWTLFIAQALMMGLSACIGGTQPAMLAEQIPTRYRTLIMGTAMPLAVALFGGTAPYIN
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
TWLASIDMFWLFDSYIIAMTLGTAIVVGFRWKETKGIHLVDVR
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEEC
>Mature Secondary Structure 
TTSTADITPALSSSPASNATRRQSLLATGVGNVLEWFDWAVYGVFSTYIAMAMFNQDNP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
VSALLSTLAVFAVGFVMRPLGGFVFGRIADRKGRKWVLLVTMLMMASGSLLIGIIPSYET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCHHH
IGGFASFLLLLARLIQGFAHGGEATASNVYLPEIAPRHRRALYGSTIGFAMGLGTMIAIL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHH
FGSVLTNIFDSAVMNEWGWRIPFIFGGLLAVVVLWLRRNMMESEVHEAHVEIAQMANDVE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENPSLAQVKTAATDEASKVNVVVEWPNRKIFVKAVEVFFYMAGTTLPYYIWSSFAATYAI
CCCCHHHEEHHCCCCCCEEEEEEECCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHEE
TQKDMDPAGAFTASLGAMAVNLALVPIMGLIADKIGRRIPVLVYALATAALTVPVFAMIN
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEEC
DNPWTLFIAQALMMGLSACIGGTQPAMLAEQIPTRYRTLIMGTAMPLAVALFGGTAPYIN
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
TWLASIDMFWLFDSYIIAMTLGTAIVVGFRWKETKGIHLVDVR
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]