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The map label for this gene is tig

Identifier: 145296395

GI number: 145296395

Start: 2543233

End: 2544582

Strand: Reverse

Name: tig

Synonym: cgR_2309

Alternate gene names: 145296395

Gene position: 2544582-2543233 (Counterclockwise)

Preceding gene: 145296400

Following gene: 145296394

Centisome position: 76.78

GC content: 55.93

Gene sequence:

>1350_bases
GTGAAGAGTTCTGTCGAGAAGCTGAGCGACACCCGTTCAAAGATCACCGTTGAGGTTCCTTTTTCTGAACTGAAGCCAGA
GATCGACCAGGCATACGCCGCTCTAGCGCAGCAAGTCCAGATCCCTGGTTTCCGTAAGGGCAAGGCGCCGCGTCAGCTTA
TCGACGCACGCTTCGGCCGTGGCGCAGTTCTGGAGCAGGTTGTCAACGACATGCTTCCTAACCGCTACGCACAGGCAATC
GAAGCTGAGGGCATCAAGGCAATCGGTCAGCCTAACGTAGAGGTCACGAAGATCGAAGACAACGAGCTCGTTGAGTTCGT
CGCTGAGGTTGACGTTCGCCCAGAGTTCGAGCTTCCTAAGTTCGAGGACATCACTGTTGAGGTCCCAGCTATCAAGGCTG
ACGAAGAGGCAATCGAAGCAGAGCTCGAGACCCTGCGTGCACGTTTCTCCACCTTGAAGGATCACAACCACAAGCTGAAG
AAGGGTGAGTTCGTCACCATCAACATCACCGCAAGCATTGACGGTGAGAAGATTGAAGAGGCAACCACTGAGGGTCTGTC
CTACGAAATCGGATCTGACGATCTGATCGACGGCCTGGACAAGGCTCTGATCGGCGCTAAGAAGGATGAAACCGTAGAGT
TCACCTCTGAGCTGGCAAACGGCGAGCACAAGGGCAAGGAAGCTCAAATCAGCGTTGAGATCACCGCAACCAAGCAGCGC
GAGCTGCCTGAGCTGGATGATGAGTTCGCACAGCTGGCTTCTGAGTTCGACACCATCGAAGAGCTTCGTGAGTCCACCGT
GTCTGACGTTGAGGCTAAGCAGAAGAACGAGCAGGCTGCTGCAATCCGCGACGAAGTTCTCGCTGCGGCTCTTGGCGAGG
CTGACTTCGCTCTGCCACAGTCCATCGTTGACGAGCAGGCACACTCCCAGCTGCACCAGCTCCTCGGCGAGCTTGCACAC
GACGATGCTGCACTGAACTCCCTCCTTGAGGCTCAGGGCACCACTCGTGAAGAGTTCGACAAGAAGAACGTCGAAGATGC
TGAGAAGGCTGTTCGCACCCAGCTGTTCCTGGACACCCTCTCTGAGGTTGAGGAGCCTGAGGTTTCCCAGCAGGAGCTCA
CCGACCACATCCTGTTCACCGCACAGTCTTACGGCATGGACCCAAACCAGTTCATCGGTCAGCTGCAGCAGTCCGGCCAG
ATCGCGAACCTCTTCTCCGACGTTCGCCGTGGCAAGGCTCTTGCACAGGCTATCTGCCGCGTAAACGTGAAGGACTCCGA
GGGTAACGAGATCGACCCTAAGGAATACTTCGGTGAAGAAGAAGTAGCTGAGACTGAGTCTGAAGCTTAA

Upstream 100 bases:

>100_bases
GTTTCGGACGACGCGAGAAATCGCATTAATCGTCGGAAACCGGGCTTGTTTTTGTAATATCTGAAACTTTCCCTTTCCCG
ATCATCCAGGAGATTTACTC

Downstream 100 bases:

>100_bases
AAACTTTAAAGAAATAACGCTTTTCGACGCGCCACTGGACGTCGAGAAGCGTTATTTTTTTTGCTTTTTTAAACGGTGAA
AACGCTGATAGCGAACAGAG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MKSSVEKLSDTRSKITVEVPFSELKPEIDQAYAALAQQVQIPGFRKGKAPRQLIDARFGRGAVLEQVVNDMLPNRYAQAI
EAEGIKAIGQPNVEVTKIEDNELVEFVAEVDVRPEFELPKFEDITVEVPAIKADEEAIEAELETLRARFSTLKDHNHKLK
KGEFVTINITASIDGEKIEEATTEGLSYEIGSDDLIDGLDKALIGAKKDETVEFTSELANGEHKGKEAQISVEITATKQR
ELPELDDEFAQLASEFDTIEELRESTVSDVEAKQKNEQAAAIRDEVLAAALGEADFALPQSIVDEQAHSQLHQLLGELAH
DDAALNSLLEAQGTTREEFDKKNVEDAEKAVRTQLFLDTLSEVEEPEVSQQELTDHILFTAQSYGMDPNQFIGQLQQSGQ
IANLFSDVRRGKALAQAICRVNVKDSEGNEIDPKEYFGEEEVAETESEA

Sequences:

>Translated_449_residues
MKSSVEKLSDTRSKITVEVPFSELKPEIDQAYAALAQQVQIPGFRKGKAPRQLIDARFGRGAVLEQVVNDMLPNRYAQAI
EAEGIKAIGQPNVEVTKIEDNELVEFVAEVDVRPEFELPKFEDITVEVPAIKADEEAIEAELETLRARFSTLKDHNHKLK
KGEFVTINITASIDGEKIEEATTEGLSYEIGSDDLIDGLDKALIGAKKDETVEFTSELANGEHKGKEAQISVEITATKQR
ELPELDDEFAQLASEFDTIEELRESTVSDVEAKQKNEQAAAIRDEVLAAALGEADFALPQSIVDEQAHSQLHQLLGELAH
DDAALNSLLEAQGTTREEFDKKNVEDAEKAVRTQLFLDTLSEVEEPEVSQQELTDHILFTAQSYGMDPNQFIGQLQQSGQ
IANLFSDVRRGKALAQAICRVNVKDSEGNEIDPKEYFGEEEVAETESEA
>Mature_449_residues
MKSSVEKLSDTRSKITVEVPFSELKPEIDQAYAALAQQVQIPGFRKGKAPRQLIDARFGRGAVLEQVVNDMLPNRYAQAI
EAEGIKAIGQPNVEVTKIEDNELVEFVAEVDVRPEFELPKFEDITVEVPAIKADEEAIEAELETLRARFSTLKDHNHKLK
KGEFVTINITASIDGEKIEEATTEGLSYEIGSDDLIDGLDKALIGAKKDETVEFTSELANGEHKGKEAQISVEITATKQR
ELPELDDEFAQLASEFDTIEELRESTVSDVEAKQKNEQAAAIRDEVLAAALGEADFALPQSIVDEQAHSQLHQLLGELAH
DDAALNSLLEAQGTTREEFDKKNVEDAEKAVRTQLFLDTLSEVEEPEVSQQELTDHILFTAQSYGMDPNQFIGQLQQSGQ
IANLFSDVRRGKALAQAICRVNVKDSEGNEIDPKEYFGEEEVAETESEA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=315, Percent_Identity=27.9365079365079, Blast_Score=119, Evalue=4e-28,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_CORGB (A4QGE8)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001139216.1
- ProteinModelPortal:   A4QGE8
- STRING:   A4QGE8
- GeneID:   4991757
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2309
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 49699; Mature: 49699

Theoretical pI: Translated: 4.12; Mature: 4.12

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSVEKLSDTRSKITVEVPFSELKPEIDQAYAALAQQVQIPGFRKGKAPRQLIDARFGR
CCCHHHHHHHHHHEEEEEECHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCC
GAVLEQVVNDMLPNRYAQAIEAEGIKAIGQPNVEVTKIEDNELVEFVAEVDVRPEFELPK
HHHHHHHHHHHCCHHHHHHHHHCCCHHCCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCC
FEDITVEVPAIKADEEAIEAELETLRARFSTLKDHNHKLKKGEFVTINITASIDGEKIEE
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCHHHHH
ATTEGLSYEIGSDDLIDGLDKALIGAKKDETVEFTSELANGEHKGKEAQISVEITATKQR
HHHCCCEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEECCCC
ELPELDDEFAQLASEFDTIEELRESTVSDVEAKQKNEQAAAIRDEVLAAALGEADFALPQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCH
SIVDEQAHSQLHQLLGELAHDDAALNSLLEAQGTTREEFDKKNVEDAEKAVRTQLFLDTL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHH
SEVEEPEVSQQELTDHILFTAQSYGMDPNQFIGQLQQSGQIANLFSDVRRGKALAQAICR
HHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VNVKDSEGNEIDPKEYFGEEEVAETESEA
CCCCCCCCCCCCHHHHCCCHHHHHCCCCC
>Mature Secondary Structure
MKSSVEKLSDTRSKITVEVPFSELKPEIDQAYAALAQQVQIPGFRKGKAPRQLIDARFGR
CCCHHHHHHHHHHEEEEEECHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCC
GAVLEQVVNDMLPNRYAQAIEAEGIKAIGQPNVEVTKIEDNELVEFVAEVDVRPEFELPK
HHHHHHHHHHHCCHHHHHHHHHCCCHHCCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCC
FEDITVEVPAIKADEEAIEAELETLRARFSTLKDHNHKLKKGEFVTINITASIDGEKIEE
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCHHHHH
ATTEGLSYEIGSDDLIDGLDKALIGAKKDETVEFTSELANGEHKGKEAQISVEITATKQR
HHHCCCEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEECCCC
ELPELDDEFAQLASEFDTIEELRESTVSDVEAKQKNEQAAAIRDEVLAAALGEADFALPQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCH
SIVDEQAHSQLHQLLGELAHDDAALNSLLEAQGTTREEFDKKNVEDAEKAVRTQLFLDTL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHH
SEVEEPEVSQQELTDHILFTAQSYGMDPNQFIGQLQQSGQIANLFSDVRRGKALAQAICR
HHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VNVKDSEGNEIDPKEYFGEEEVAETESEA
CCCCCCCCCCCCHHHHCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA