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The map label for this gene is lepA [H]

Identifier: 145296308

GI number: 145296308

Start: 2454256

End: 2454576

Strand: Reverse

Name: lepA [H]

Synonym: cgR_2223

Alternate gene names: 145296308

Gene position: 2454576-2454256 (Counterclockwise)

Preceding gene: 145296312

Following gene: 145296307

Centisome position: 74.06

GC content: 52.65

Gene sequence:

>321_bases
ATGGATGCATTCTCTGCGATCGTGCACCGCGATAATGCGCAGTGGTACGGAAACAAGATGACTGTGAAGCTGAAGGAACT
GATCCCTCGCCAGCAGTTCGAAGTTCCTGTGCAGGCAGCCATTGGTTCCAAGGTTATCGCTCGTGAAAACATTCGTGCAC
TGCGCAAGGACGTGTTGGCGAAGTGTTACGGTGGCGATATTTCCCGTAAGCGCAAGCTTCTGGAAAAGCAGAAGGCTGGT
AAGAAGCGCATGAAGAACATCGGTTCGGTCGAGGTTCCTCAGGAGGCATTCGTAGCAGCACTGTCTACCGACGAGGCATA
A

Upstream 100 bases:

>100_bases
TCTCGCACCAAGGGTTACGCCTCCCTGAACTACGAGGAAGCCGGCGAGCAGACTGCCGACCTGGTCAAGGTAGATATCTT
GCTCCAAGGTGAACCTGTGG

Downstream 100 bases:

>100_bases
AAAACTTTAGCCTCTTTTAACGCCCCGCTGAGCTTGCAGCTCAGCGGGGCGTTGTCGTGTTTCGGTGTGGGGGAGCGTCG
AAAAGCGAAAGTATCTAAGG

Product: hypothetical protein

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 106; Mature: 106

Protein sequence:

>106_residues
MDAFSAIVHRDNAQWYGNKMTVKLKELIPRQQFEVPVQAAIGSKVIARENIRALRKDVLAKCYGGDISRKRKLLEKQKAG
KKRMKNIGSVEVPQEAFVAALSTDEA

Sequences:

>Translated_106_residues
MDAFSAIVHRDNAQWYGNKMTVKLKELIPRQQFEVPVQAAIGSKVIARENIRALRKDVLAKCYGGDISRKRKLLEKQKAG
KKRMKNIGSVEVPQEAFVAALSTDEA
>Mature_106_residues
MDAFSAIVHRDNAQWYGNKMTVKLKELIPRQQFEVPVQAAIGSKVIARENIRALRKDVLAKCYGGDISRKRKLLEKQKAG
KKRMKNIGSVEVPQEAFVAALSTDEA

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=101, Percent_Identity=54.4554455445545, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1788922, Length=101, Percent_Identity=64.3564356435644, Blast_Score=126, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17557151, Length=104, Percent_Identity=38.4615384615385, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=101, Percent_Identity=45.5445544554455, Blast_Score=94, Evalue=5e-21,
Organism=Drosophila melanogaster, GI78706572, Length=95, Percent_Identity=49.4736842105263, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 11898; Mature: 11898

Theoretical pI: Translated: 10.64; Mature: 10.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAFSAIVHRDNAQWYGNKMTVKLKELIPRQQFEVPVQAAIGSKVIARENIRALRKDVLA
CCHHHHHHHCCCCCCCCCCCEEEHHHHCCHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHH
KCYGGDISRKRKLLEKQKAGKKRMKNIGSVEVPQEAFVAALSTDEA
HHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MDAFSAIVHRDNAQWYGNKMTVKLKELIPRQQFEVPVQAAIGSKVIARENIRALRKDVLA
CCHHHHHHHCCCCCCCCCCCEEEHHHHCCHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHH
KCYGGDISRKRKLLEKQKAGKKRMKNIGSVEVPQEAFVAALSTDEA
HHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 14602910 [H]