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The map label for this gene is brnQ [H]

Identifier: 145296265

GI number: 145296265

Start: 2407445

End: 2408692

Strand: Direct

Name: brnQ [H]

Synonym: cgR_2182

Alternate gene names: 145296265

Gene position: 2407445-2408692 (Clockwise)

Preceding gene: 145296264

Following gene: 145296266

Centisome position: 72.64

GC content: 53.21

Gene sequence:

>1248_bases
ATGCTGTTTTCCATGTTCTTTGGAGCTGGAAACCTCATCTTCCCGCCGATGCTTGGATTGTCGGCAGGAACCAACTATCT
ACCAGCTATCTTAGGATTTCTAGCAACGAGTGTTCTGCTCCCGGTGCTGGCGATTATCGCGGTGGTGTTGTCGGGAGAGA
ATGTCAAGGACATGGCTTCTCGTGGCGGTAAGATCTTTGGCCTGGTGTTTCCTATTGCTGCCTATTTGTCCATCGGCGCG
TTTTACGCGCTGCCGAGGACTGGGGCGGTGAGCTATTCGACGGCGGTTGGCGTCGATAATGCGCTTTATTCGGGCTTGTT
TAACTTTGTGTTTTTTGCGGTGGCACTGGCGTTGTCGTGGAATCCGAATGGCATTGCAGACAAATTGGGTAAGTGGCTCA
CGCCAGCGTTGCTCACATTGATTGTGGTGCTGGTGGTGTTGTCGGTAGCCAAGTTGGATGGCGCGCCGGGCGAGCCGAGC
AGTGCGTATGCGCAGCAACCTGCGGGGGCGGGTTTGCTTGAGGGCTACATGACGATGGATGCGATTGCTGCGTTGGCGTT
TGGCATCGTGGTGATTTCTGCGTTCAAGTACCAAAAGGTTAACAAGGTCCGCACGGCAACTGTCGTGTCGGCGTTCATTG
CCGGAATTTTGTTGGCGCTGGTTTATCTTGGTTTGGGCTCAATCGGTCAAGTAGTAAACGGTGAGTTCGCTGATGGCACC
GCAATTTTGAACTACGCTGCACTGTCCACGATGGGTCAGGCTGGTCGCATCATGTTCGTGGCTATTTTGATCCTTGCATG
TATGACCACCGCAGTTGGTCTGATCAGTGCAACGTCGGAGTTTTTCAATTCGTTGCTGCCAGGTGTCAAGTACCACGTCT
GGGCCACTGTTTTCGCGCTGATTTCCTTTGGCGTTGCCACCATGGGCTTGGATACGGTGTTGGCCGTTGCGGCTCCAGTG
ATTAGTTTCATTTACCCATCGGCCATCACCTTGGTGTTCTTGACGCTGATCGAGCCGCTGCTGTTCCGTCTCAAGTGGAC
CTACCTATTCGGCATTTGGACTGCAGTTGTGTGGGCGCTGTTCATGTCTATCCCTGCGCTGAATCCATTCATCGATTGGG
CGCCGCTGCACAGCATGTCTTTGGGTTGGGTTGTCCCAGTTCTCGTGGCCTCTGCCATCGGTTTGGCTATTGATTGGAAC
AAGAAAGGTACCCAGTCTGTTGCAAAGGAGGAATCCATTTCCGTCTAA

Upstream 100 bases:

>100_bases
TAATTTTGCGTGTTCCAGAGAGACCCTTGAGGAGGGGCTGCGCCGTATCGCCAGCGTGTTGTAAATAATGAGTAAAAAGT
CTGTCCTGATTACTTCTTTG

Downstream 100 bases:

>100_bases
TCGCTAATTGCGAGGAGTCTTTGCATGTCTATCCCACTTTCACTGATTGATTTTGCCACCATTTTTGAGGGCGAAAGGCC
TGGTGACAGCTTCAAACGAT

Product: hypothetical protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MLFSMFFGAGNLIFPPMLGLSAGTNYLPAILGFLATSVLLPVLAIIAVVLSGENVKDMASRGGKIFGLVFPIAAYLSIGA
FYALPRTGAVSYSTAVGVDNALYSGLFNFVFFAVALALSWNPNGIADKLGKWLTPALLTLIVVLVVLSVAKLDGAPGEPS
SAYAQQPAGAGLLEGYMTMDAIAALAFGIVVISAFKYQKVNKVRTATVVSAFIAGILLALVYLGLGSIGQVVNGEFADGT
AILNYAALSTMGQAGRIMFVAILILACMTTAVGLISATSEFFNSLLPGVKYHVWATVFALISFGVATMGLDTVLAVAAPV
ISFIYPSAITLVFLTLIEPLLFRLKWTYLFGIWTAVVWALFMSIPALNPFIDWAPLHSMSLGWVVPVLVASAIGLAIDWN
KKGTQSVAKEESISV

Sequences:

>Translated_415_residues
MLFSMFFGAGNLIFPPMLGLSAGTNYLPAILGFLATSVLLPVLAIIAVVLSGENVKDMASRGGKIFGLVFPIAAYLSIGA
FYALPRTGAVSYSTAVGVDNALYSGLFNFVFFAVALALSWNPNGIADKLGKWLTPALLTLIVVLVVLSVAKLDGAPGEPS
SAYAQQPAGAGLLEGYMTMDAIAALAFGIVVISAFKYQKVNKVRTATVVSAFIAGILLALVYLGLGSIGQVVNGEFADGT
AILNYAALSTMGQAGRIMFVAILILACMTTAVGLISATSEFFNSLLPGVKYHVWATVFALISFGVATMGLDTVLAVAAPV
ISFIYPSAITLVFLTLIEPLLFRLKWTYLFGIWTAVVWALFMSIPALNPFIDWAPLHSMSLGWVVPVLVASAIGLAIDWN
KKGTQSVAKEESISV
>Mature_415_residues
MLFSMFFGAGNLIFPPMLGLSAGTNYLPAILGFLATSVLLPVLAIIAVVLSGENVKDMASRGGKIFGLVFPIAAYLSIGA
FYALPRTGAVSYSTAVGVDNALYSGLFNFVFFAVALALSWNPNGIADKLGKWLTPALLTLIVVLVVLSVAKLDGAPGEPS
SAYAQQPAGAGLLEGYMTMDAIAALAFGIVVISAFKYQKVNKVRTATVVSAFIAGILLALVYLGLGSIGQVVNGEFADGT
AILNYAALSTMGQAGRIMFVAILILACMTTAVGLISATSEFFNSLLPGVKYHVWATVFALISFGVATMGLDTVLAVAAPV
ISFIYPSAITLVFLTLIEPLLFRLKWTYLFGIWTAVVWALFMSIPALNPFIDWAPLHSMSLGWVVPVLVASAIGLAIDWN
KKGTQSVAKEESISV

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=433, Percent_Identity=33.256351039261, Blast_Score=201, Evalue=8e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 43645; Mature: 43645

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFSMFFGAGNLIFPPMLGLSAGTNYLPAILGFLATSVLLPVLAIIAVVLSGENVKDMAS
CEEEEEECCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
RGGKIFGLVFPIAAYLSIGAFYALPRTGAVSYSTAVGVDNALYSGLFNFVFFAVALALSW
CCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
NPNGIADKLGKWLTPALLTLIVVLVVLSVAKLDGAPGEPSSAYAQQPAGAGLLEGYMTMD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHH
AIAALAFGIVVISAFKYQKVNKVRTATVVSAFIAGILLALVYLGLGSIGQVVNGEFADGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCH
AILNYAALSTMGQAGRIMFVAILILACMTTAVGLISATSEFFNSLLPGVKYHVWATVFAL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ISFGVATMGLDTVLAVAAPVISFIYPSAITLVFLTLIEPLLFRLKWTYLFGIWTAVVWAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FMSIPALNPFIDWAPLHSMSLGWVVPVLVASAIGLAIDWNKKGTQSVAKEESISV
HHHCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MLFSMFFGAGNLIFPPMLGLSAGTNYLPAILGFLATSVLLPVLAIIAVVLSGENVKDMAS
CEEEEEECCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
RGGKIFGLVFPIAAYLSIGAFYALPRTGAVSYSTAVGVDNALYSGLFNFVFFAVALALSW
CCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
NPNGIADKLGKWLTPALLTLIVVLVVLSVAKLDGAPGEPSSAYAQQPAGAGLLEGYMTMD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHH
AIAALAFGIVVISAFKYQKVNKVRTATVVSAFIAGILLALVYLGLGSIGQVVNGEFADGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCH
AILNYAALSTMGQAGRIMFVAILILACMTTAVGLISATSEFFNSLLPGVKYHVWATVFAL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ISFGVATMGLDTVLAVAAPVISFIYPSAITLVFLTLIEPLLFRLKWTYLFGIWTAVVWAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FMSIPALNPFIDWAPLHSMSLGWVVPVLVASAIGLAIDWNKKGTQSVAKEESISV
HHHCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]