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The map label for this gene is lipA

Identifier: 145296170

GI number: 145296170

Start: 2306320

End: 2307366

Strand: Direct

Name: lipA

Synonym: cgR_2089

Alternate gene names: 145296170

Gene position: 2306320-2307366 (Clockwise)

Preceding gene: 145296169

Following gene: 145296171

Centisome position: 69.59

GC content: 55.4

Gene sequence:

>1047_bases
GTGACTATCGCACCTGAAGGACGACGACTGCTACGCGTCGAAGCTCGAAACTCAGAAACCCCGATTGAGACGAAGCCTCG
ATGGATTAGAAACCAGGTCAAAAACGGACCTGAGTATCAGGATATGAAGGAACGTGTCGCTGGCGCATCACTACACACGG
TGTGCCAGGAGGCTGGCTGTCCTAATATCCATGAGTGTTGGGAATCCCGTGAGGCAACCTTCCTCATTGGTGGCGCCAAC
TGCTCTCGCCGCTGTGATTTCTGCATGATCAACTCGGCTCGCCCTGAGCCACTCGACCGCGGTGAGCCACTGCGTGTCGC
TGAGTCTGTTCGTGAGATGCAGCTGAATTACTCCACCATCACCGGTGTTACTCGTGATGATCTGGATGATGAAGGCGCAT
GGCTGTACTCAGAAGTGGTTCGTAAGATCCACGAGCTGAACCCACACACCGGTGTGGAAAACCTGGTGCCTGATTTCTCC
GGCAAGAAGGATCTGCTGCAGGAAGTTTTTGAATCCCGCCCAGAGGTTTTCGCTCACAACGTGGAAACTGTGCCACGTAT
TTTCAAGCGCATTCGCCCAGCATTCCGCTACGAGCGTTCACTTGATGTGATCCGTCAGGCTCGCGATTTCGGTCTGGTGA
CCAAGTCCAACCTGATTTTGGGCATGGGTGAAACCAAGGAAGAAATCACCGAGGCGCTGCAGGATCTGCACGACGCTGGC
TGTGACATCATCACCATCACCCAGTACCTGCGTCCTGGTCCTTTGTTCCACCCCATCGAGCGTTGGGTGAAGCCTGAGGA
GTTCCTCGAGCACGCTGATGCTGCAAAGGAAATGGGCTTCGCTGCTGTTATGTCCGGCCCATTGGTTCGTTCCTCTTACC
GTGCAGGCCGTCTGTACGCGCAGGCCATGGAGTTCCGTGGCGAGGAAATCCCAGCACACCTCGCGCACCTGAAGGATACT
TCCGGAGGATCCACCGCCCAGGAAGCATCTACACTTCTGGAGCGTTACGGTGCTTCCGAAGACACCCCAGTGGTGTCCTT
CAACTAA

Upstream 100 bases:

>100_bases
CGAAGTAGAGCCGATTGCAGAATCGGCGGAATGAGACGTCGAAAAGCGTTTAAGCTTTCCCTAAAAATATCACTAACTCG
AAAGATGTAAGGTTGCATTT

Downstream 100 bases:

>100_bases
GCCCGAAGTTTTCTTAACCGCCGCATTCGATCACCAAATGTGGCGGTTTTGCGTCGAAAAGCCTGCTCTTTCTACACCTC
TTTGAGGTTCATTTTCGCGG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MTIAPEGRRLLRVEARNSETPIETKPRWIRNQVKNGPEYQDMKERVAGASLHTVCQEAGCPNIHECWESREATFLIGGAN
CSRRCDFCMINSARPEPLDRGEPLRVAESVREMQLNYSTITGVTRDDLDDEGAWLYSEVVRKIHELNPHTGVENLVPDFS
GKKDLLQEVFESRPEVFAHNVETVPRIFKRIRPAFRYERSLDVIRQARDFGLVTKSNLILGMGETKEEITEALQDLHDAG
CDIITITQYLRPGPLFHPIERWVKPEEFLEHADAAKEMGFAAVMSGPLVRSSYRAGRLYAQAMEFRGEEIPAHLAHLKDT
SGGSTAQEASTLLERYGASEDTPVVSFN

Sequences:

>Translated_348_residues
MTIAPEGRRLLRVEARNSETPIETKPRWIRNQVKNGPEYQDMKERVAGASLHTVCQEAGCPNIHECWESREATFLIGGAN
CSRRCDFCMINSARPEPLDRGEPLRVAESVREMQLNYSTITGVTRDDLDDEGAWLYSEVVRKIHELNPHTGVENLVPDFS
GKKDLLQEVFESRPEVFAHNVETVPRIFKRIRPAFRYERSLDVIRQARDFGLVTKSNLILGMGETKEEITEALQDLHDAG
CDIITITQYLRPGPLFHPIERWVKPEEFLEHADAAKEMGFAAVMSGPLVRSSYRAGRLYAQAMEFRGEEIPAHLAHLKDT
SGGSTAQEASTLLERYGASEDTPVVSFN
>Mature_347_residues
TIAPEGRRLLRVEARNSETPIETKPRWIRNQVKNGPEYQDMKERVAGASLHTVCQEAGCPNIHECWESREATFLIGGANC
SRRCDFCMINSARPEPLDRGEPLRVAESVREMQLNYSTITGVTRDDLDDEGAWLYSEVVRKIHELNPHTGVENLVPDFSG
KKDLLQEVFESRPEVFAHNVETVPRIFKRIRPAFRYERSLDVIRQARDFGLVTKSNLILGMGETKEEITEALQDLHDAGC
DIITITQYLRPGPLFHPIERWVKPEEFLEHADAAKEMGFAAVMSGPLVRSSYRAGRLYAQAMEFRGEEIPAHLAHLKDTS
GGSTAQEASTLLERYGASEDTPVVSFN

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=287, Percent_Identity=37.2822299651568, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI37577164, Length=236, Percent_Identity=37.7118644067797, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1786846, Length=286, Percent_Identity=40.5594405594406, Blast_Score=228, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI32564533, Length=258, Percent_Identity=40.3100775193798, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6324770, Length=297, Percent_Identity=39.0572390572391, Blast_Score=212, Evalue=9e-56,
Organism=Drosophila melanogaster, GI221513272, Length=287, Percent_Identity=39.3728222996516, Blast_Score=214, Evalue=5e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_CORGB (A4QFS3)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138991.1
- ProteinModelPortal:   A4QFS3
- STRING:   A4QFS3
- GeneID:   4992088
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_2089
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 39312; Mature: 39181

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAPEGRRLLRVEARNSETPIETKPRWIRNQVKNGPEYQDMKERVAGASLHTVCQEAGC
CCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHCCC
PNIHECWESREATFLIGGANCSRRCDFCMINSARPEPLDRGEPLRVAESVREMQLNYSTI
CCHHHHHCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCHHHH
TGVTRDDLDDEGAWLYSEVVRKIHELNPHTGVENLVPDFSGKKDLLQEVFESRPEVFAHN
CCCHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHCCHHHHHCC
VETVPRIFKRIRPAFRYERSLDVIRQARDFGLVTKSNLILGMGETKEEITEALQDLHDAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCEEEECCCCHHHHHHHHHHHHHCC
CDIITITQYLRPGPLFHPIERWVKPEEFLEHADAAKEMGFAAVMSGPLVRSSYRAGRLYA
CCEEEHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
QAMEFRGEEIPAHLAHLKDTSGGSTAQEASTLLERYGASEDTPVVSFN
HHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEECC
>Mature Secondary Structure 
TIAPEGRRLLRVEARNSETPIETKPRWIRNQVKNGPEYQDMKERVAGASLHTVCQEAGC
CCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHCCC
PNIHECWESREATFLIGGANCSRRCDFCMINSARPEPLDRGEPLRVAESVREMQLNYSTI
CCHHHHHCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCHHHH
TGVTRDDLDDEGAWLYSEVVRKIHELNPHTGVENLVPDFSGKKDLLQEVFESRPEVFAHN
CCCHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHCCHHHHHCC
VETVPRIFKRIRPAFRYERSLDVIRQARDFGLVTKSNLILGMGETKEEITEALQDLHDAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCEEEECCCCHHHHHHHHHHHHHCC
CDIITITQYLRPGPLFHPIERWVKPEEFLEHADAAKEMGFAAVMSGPLVRSSYRAGRLYA
CCEEEHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
QAMEFRGEEIPAHLAHLKDTSGGSTAQEASTLLERYGASEDTPVVSFN
HHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA